This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
LEAF™ Purified anti-mouse CD28
catalog :
102112
quantity :
500 μg
price :
230 USD
clonality :
monoclonal
host :
golden hamster
conjugate :
nonconjugated
clone name :
37.51
reactivity :
human, mouse
application :
neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
citations: 38
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 1b
Humeniuk P, Geiselhart S, Battin C, Webb T, Steinberger P, Paster W, et al. Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor. Sci Rep. 2019;9:7426 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig 3a
Li B, Wang X, Choi I, Wang Y, Liu S, Pham A, et al. miR-146a modulates autoreactive Th17 cell differentiation and regulates organ-specific autoimmunity. J Clin Invest. 2017;127:3702-3716 pubmed publisher
  • blocking or activating experiments; mouse; fig s2a
Chien C, Yu H, Chen S, Chiang B. Characterization of c-Maf+Foxp3- Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells. Sci Rep. 2017;7:46348 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig 5a
Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
  • blocking or activating experiments; mouse; 2 ug/ml; loading ...; fig 3b
González Pérez G, Lamousé Smith E. Gastrointestinal Microbiome Dysbiosis in Infant Mice Alters Peripheral CD8+ T Cell Receptor Signaling. Front Immunol. 2017;8:265 pubmed publisher
  • blocking or activating experiments; mouse; fig 5c
Klein J, Moses K, Zelinskyy G, Sody S, Buer J, Lang S, et al. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth. Nat Commun. 2017;8:14600 pubmed publisher
  • flow cytometry; mouse; 5 ug/ml; loading ...; fig 4A
Troegeler A, Mercier I, Cougoule C, Pietretti D, Colom A, Duval C, et al. C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells. Proc Natl Acad Sci U S A. 2017;114:E540-E549 pubmed publisher
  • ELISA; mouse; loading ...
Cecchinato V, Bernasconi E, Speck R, Proietti M, Sauermann U, D Agostino G, et al. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization. J Immunol. 2017;198:184-195 pubmed
  • blocking or activating experiments; mouse; 5 ug/ml
Bieber K, Witte M, Sun S, Hundt J, Kalies K, Dräger S, et al. T cells mediate autoantibody-induced cutaneous inflammation and blistering in epidermolysis bullosa acquisita. Sci Rep. 2016;6:38357 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig 5a
Ishikura S, Tsunoda T, Nakabayashi K, Doi K, Koyanagi M, Hayashi K, et al. Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells. Biochim Biophys Acta. 2016;1859:1398-1410 pubmed publisher
  • blocking or activating experiments; mouse; 2 ug/ml; loading ...; fig 2a
Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
  • blocking or activating experiments; mouse; fig 1
Nagashima H, Okuyama Y, Hayashi T, Ishii N, So T. TNFR-Associated Factors 2 and 5 Differentially Regulate the Instructive IL-6 Receptor Signaling Required for Th17 Development. J Immunol. 2016;196:4082-9 pubmed publisher
  • blocking or activating experiments; mouse; 2 ug/ml; fig 2
Du C, Duan Y, Wei W, Cai Y, Chai H, Lv J, et al. Kappa opioid receptor activation alleviates experimental autoimmune encephalomyelitis and promotes oligodendrocyte-mediated remyelination. Nat Commun. 2016;7:11120 pubmed publisher
  • blocking or activating experiments; mouse; fig 3
Apostolidis S, Rodríguez Rodríguez N, Suárez Fueyo A, Dioufa N, Ozcan E, Crispín J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
  • blocking or activating experiments; mouse
Hwang S, Jang S, Kim M, Kim L, Kim B, Kim H, et al. YY1 inhibits differentiation and function of regulatory T cells by blocking Foxp3 expression and activity. Nat Commun. 2016;7:10789 pubmed publisher
  • blocking or activating experiments; mouse; 2 ug/ml
Kimmerling R, Lee Szeto G, Li J, Genshaft A, Kazer S, Payer K, et al. A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages. Nat Commun. 2016;7:10220 pubmed publisher
  • blocking or activating experiments; human; fig 1
McCully M, Collins P, Hughes T, Thomas C, Billen J, O Donnell V, et al. Skin Metabolites Define a New Paradigm in the Localization of Skin Tropic Memory T Cells. J Immunol. 2015;195:96-104 pubmed publisher
  • immunoprecipitation; mouse
Richardson E, Shukla S, Sweet D, Wearsch P, Tsichlis P, Boom W, et al. Toll-like receptor 2-dependent extracellular signal-regulated kinase signaling in Mycobacterium tuberculosis-infected macrophages drives anti-inflammatory responses and inhibits Th1 polarization of responding T cells. Infect Immun. 2015;83:2242-54 pubmed publisher
  • blocking or activating experiments; mouse; 2 ug/ml
Rao E, Zhang Y, Zhu G, Hao J, Persson X, Egilmez N, et al. Deficiency of AMPK in CD8+ T cells suppresses their anti-tumor function by inducing protein phosphatase-mediated cell death. Oncotarget. 2015;6:7944-58 pubmed
  • blocking or activating experiments; mouse; 20 ug/ml; fig 1
Fos C, Bécart S, Canonigo Balancio A, Boehning D, Altman A. Association of the EF-hand and PH domains of the guanine nucleotide exchange factor SLAT with IP₃ receptor 1 promotes Ca²⁺ signaling in T cells. Sci Signal. 2014;7:ra93 pubmed publisher
  • blocking or activating experiments; mouse; 0.5 ug/ml
Koga T, Hedrich C, Mizui M, Yoshida N, Otomo K, Lieberman L, et al. CaMK4-dependent activation of AKT/mTOR and CREM-? underlies autoimmunity-associated Th17 imbalance. J Clin Invest. 2014;124:2234-45 pubmed publisher
  • blocking or activating experiments; mouse
Nancy P, Tagliani E, Tay C, Asp P, Levy D, Erlebacher A. Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface. Science. 2012;336:1317-21 pubmed publisher
Liu B, Salgado O, Singh S, Hippen K, Maynard J, Burlingame A, et al. The lineage stability and suppressive program of regulatory T cells require protein O-GlcNAcylation. Nat Commun. 2019;10:354 pubmed publisher
Ren W, Liao Y, Ding X, Jiang Y, Yan J, Xia Y, et al. Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection. Mucosal Immunol. 2019;12:531-544 pubmed publisher
Sade Feldman M, Yizhak K, Bjorgaard S, Ray J, de Boer C, Jenkins R, et al. Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma. Cell. 2018;175:998-1013.e20 pubmed publisher
Fan M, Low J, Tanimine N, Finn K, Priyadharshini B, Germana S, et al. Differential Roles of IL-2 Signaling in Developing versus Mature Tregs. Cell Rep. 2018;25:1204-1213.e4 pubmed publisher
Torgovnick A, Heger J, Liaki V, Isensee J, Schmitt A, Knittel G, et al. The Cdkn1aSUPER Mouse as a Tool to Study p53-Mediated Tumor Suppression. Cell Rep. 2018;25:1027-1039.e6 pubmed publisher
Tsai S, Clemente Casares X, Zhou A, Lei H, Ahn J, Chan Y, et al. Insulin Receptor-Mediated Stimulation Boosts T Cell Immunity during Inflammation and Infection. Cell Metab. 2018;28:922-934.e4 pubmed publisher
Moore M, Blachere N, Fak J, Park C, Sawicka K, Parveen S, et al. ZFP36 RNA-binding proteins restrain T cell activation and anti-viral immunity. elife. 2018;7: pubmed publisher
Coley W, Zhao Y, Benck C, Liu Y, Hotta Iwamura C, Rahman M, et al. Loss of Zbtb32 in NOD mice does not significantly alter T cell responses. F1000Res. 2018;7:318 pubmed publisher
Smith L, Boukhaled G, Condotta S, Mazouz S, Guthmiller J, Vijay R, et al. Interleukin-10 Directly Inhibits CD8+ T Cell Function by Enhancing N-Glycan Branching to Decrease Antigen Sensitivity. Immunity. 2018;48:299-312.e5 pubmed publisher
Hawila E, Razon H, Wildbaum G, Blattner C, Sapir Y, Shaked Y, et al. CCR5 Directs the Mobilization of CD11b+Gr1+Ly6Clow Polymorphonuclear Myeloid Cells from the Bone Marrow to the Blood to Support Tumor Development. Cell Rep. 2017;21:2212-2222 pubmed publisher
Bradley C, Teng F, Felix K, Sano T, Naskar D, Block K, et al. Segmented Filamentous Bacteria Provoke Lung Autoimmunity by Inducing Gut-Lung Axis Th17 Cells Expressing Dual TCRs. Cell Host Microbe. 2017;22:697-704.e4 pubmed publisher
Klein Geltink R, O Sullivan D, Corrado M, Bremser A, Buck M, Buescher J, et al. Mitochondrial Priming by CD28. Cell. 2017;171:385-397.e11 pubmed publisher
Hrdinka M, Sudan K, Just S, Drobek A, Stepanek O, Schluter D, et al. Normal Development and Function of T Cells in Proline Rich 7 (Prr7) Deficient Mice. PLoS ONE. 2016;11:e0162863 pubmed publisher
Myers R, Dunaway C, Nelson M, Trevor J, Morris A, Steele C. STAT4-dependent and -independent Th2 responses correlate with protective immunity against lung infection with Pneumocystis murina. J Immunol. 2013;190:6287-94 pubmed publisher
Rybakin V, Gascoigne N. Negative selection assay based on stimulation of T cell receptor transgenic thymocytes with peptide-MHC tetramers. PLoS ONE. 2012;7:e43191 pubmed publisher
Chen H, Qin J, Wei P, Zhang J, Li Q, Fu L, et al. Effects of leukotriene B4 and prostaglandin E2 on the differentiation of murine Foxp3+ T regulatory cells and Th17 cells. Prostaglandins Leukot Essent Fatty Acids. 2009;80:195-200 pubmed publisher
product information
Antigen :
CD28
Apps. Abbrev. :
Flow Cytometry, IP, IHC-F, Costim, Block
Cat # :
102112
Clone :
37.51
Item :
LEAF™ Purified anti-mouse CD28
Isotype :
Syrian Hamster IgG
Other Names :
Tp44, T44
Size :
500 μg
Price (USD) :
230 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Syrian Hamster
Conjugate/Tag/Label :
LEAF
Immunogen :
C57BL/6 mouse T-cell lymphoma EL-4
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro costimulation of T and NK cells 1 , in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity 3,4 , in vitro induction of thymocyte differentiation 2,5-9,11 , and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102112). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102116) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg). This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
company information
BioLegend
9727 Pacific Heights Blvd.
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA