product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-mouse CD25
catalog :
102026
quantity :
100 μg
price :
292 USD
clonality :
monoclonal
host :
rat
conjugate :
APC/Cy7
clone name :
PC61
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 25
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:1000; loading ...; fig s2b
Sripada A, Sirohi K, Michalec L, Guo L, McKay J, Yadav S, et al. Sprouty2 positively regulates T cell function and airway inflammation through regulation of CSK and LCK kinases. PLoS Biol. 2021;19:e3001063 pubmed publisher
  • flow cytometry; mouse; fig 3
Pelly V, Kannan Y, Coomes S, Entwistle L, Rückerl D, Seddon B, et al. IL-4-producing ILC2s are required for the differentiation of TH2 cells following Heligmosomoides polygyrus infection. Mucosal Immunol. 2016;9:1407-1417 pubmed publisher
  • flow cytometry; mouse; fig 6
Stack G, Jones E, Marsden M, Stacey M, Snelgrove R, Lacaze P, et al. CD200 receptor restriction of myeloid cell responses antagonizes antiviral immunity and facilitates cytomegalovirus persistence within mucosal tissue. PLoS Pathog. 2015;11:e1004641 pubmed publisher
Duan H, Jing L, Jiang X, Ma Y, Wang D, Xiang J, et al. CD146 bound to LCK promotes T cell receptor signaling and antitumor immune responses in mice. J Clin Invest. 2021;131: pubmed publisher
Jaeger N, McDonough R, Rosen A, Hernandez Leyva A, Wilson N, Lint M, et al. Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell Rep. 2020;33:108331 pubmed publisher
Hering L, Katkeviciute E, Schwarzfischer M, Busenhart P, Gottier C, Mrdjen D, et al. Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance. Front Immunol. 2020;11:1856 pubmed publisher
Lebel M, Coutelier M, Galipeau M, Kleinman C, Moon J, Melichar H. Differential expression of tissue-restricted antigens among mTEC is associated with distinct autoreactive T cell fates. Nat Commun. 2020;11:3734 pubmed publisher
Di Luccia B, Ahern P, Griffin N, Cheng J, Guruge J, Byrne A, et al. Combined Prebiotic and Microbial Intervention Improves Oral Cholera Vaccination Responses in a Mouse Model of Childhood Undernutrition. Cell Host Microbe. 2020;27:899-908.e5 pubmed publisher
Angiari S, Runtsch M, Sutton C, Palsson McDermott E, Kelly B, Rana N, et al. Pharmacological Activation of Pyruvate Kinase M2 Inhibits CD4+ T Cell Pathogenicity and Suppresses Autoimmunity. Cell Metab. 2020;31:391-405.e8 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Yadava K, Medina C, Ishak H, Gurevich I, Kuipers H, Shamskhou E, et al. Natural Tr1-like cells do not confer long-term tolerogenic memory. elife. 2019;8: pubmed publisher
Eremenko E, Mittal K, Berner O, Kamenetsky N, Nemirovsky A, Elyahu Y, et al. BDNF-producing, amyloid β-specific CD4 T cells as targeted drug-delivery vehicles in Alzheimer's disease. EBioMedicine. 2019;43:424-434 pubmed publisher
Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
Isvoranu G, Surcel M, Huică R, Munteanu A, Pirvu I, Ciotaru D, et al. Natural killer cell monitoring in cutaneous melanoma - new dynamic biomarker. Oncol Lett. 2019;17:4197-4206 pubmed publisher
Chan S, Filézac de L Etang A, Rangell L, Caplazi P, Lowe J, Romeo V. A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples. PLoS ONE. 2018;13:e0207619 pubmed publisher
Li C, DiSpirito J, Zemmour D, Spallanzani R, Kuswanto W, Benoist C, et al. TCR Transgenic Mice Reveal Stepwise, Multi-site Acquisition of the Distinctive Fat-Treg Phenotype. Cell. 2018;174:285-299.e12 pubmed publisher
Olguín J, Medina Andrade I, Molina E, Vázquez A, Pacheco Fernández T, Saavedra R, et al. Early and Partial Reduction in CD4+Foxp3+ Regulatory T Cells during Colitis-Associated Colon Cancer Induces CD4+ and CD8+ T Cell Activation Inhibiting Tumorigenesis. J Cancer. 2018;9:239-249 pubmed publisher
Reismann D, Stefanowski J, Gunther R, Rakhymzhan A, Matthys R, Nützi R, et al. Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature. Nat Commun. 2017;8:2153 pubmed publisher
Lechner A, Driver I, Lee J, Conroy C, Nagle A, Locksley R, et al. Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy. Cell Stem Cell. 2017;21:120-134.e7 pubmed publisher
Galle Treger L, Suzuki Y, Patel N, Sankaranarayanan I, Aron J, Maazi H, et al. Nicotinic acetylcholine receptor agonist attenuates ILC2-dependent airway hyperreactivity. Nat Commun. 2016;7:13202 pubmed publisher
Long C, Marshall N, Lukomska E, Kashon M, Meade B, Shane H, et al. A Role for Regulatory T Cells in a Murine Model of Epicutaneous Toluene Diisocyanate Sensitization. Toxicol Sci. 2016;152:85-98 pubmed publisher
Soni C, Wong E, Domeier P, Khan T, Satoh T, Akira S, et al. B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. J Immunol. 2014;193:4400-14 pubmed publisher
Morawski P, Mehra P, Chen C, Bhatti T, Wells A. Foxp3 protein stability is regulated by cyclin-dependent kinase 2. J Biol Chem. 2013;288:24494-502 pubmed publisher
Randall K, Chan S, Ma C, Fung I, Mei Y, Yabas M, et al. DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice. J Exp Med. 2011;208:2305-20 pubmed publisher
del Rio R, Sun Y, Alard P, Tung K, Teuscher C. H2 control of natural T regulatory cell frequency in the lymph node correlates with susceptibility to day 3 thymectomy-induced autoimmune disease. J Immunol. 2011;186:382-9 pubmed publisher
product information
Antigen :
CD25
Apps. Abbrev. :
Flow Cytometry
Cat # :
102026
Clone :
PC61
Item :
APC/Cyanine7 anti-mouse CD25
Isotype :
Rat IgG1, λ
Other Names :
IL-2Rα, Ly-43, p55, Tac
Size :
100 μg
Price (USD) :
292 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
IL-2-dependent cytolytic mouse T-cell clone B6.1
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation 1,2 , in vitro blocking of IL-2 binding to low- and high-affinity receptors 1-4 , growth inhibition of IL-2-dependent T-cell lines 1-4 , in vivo depletion of CD25 + CD4 + Treg cells 5-8,10 , and immunohistochemical staining of acetone-fixed frozen sections 2 . PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 102040) with endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA