product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-mouse CD25
catalog :
102017
quantity :
100 μg
price :
215 USD
clonality :
monoclonal
host :
rat
conjugate :
AF488
clone name :
PC61
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 29
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...; fig s7f
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Clemen R, Freund E, Mrochen D, Miebach L, Schmidt A, Rauch B, et al. Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice. Adv Sci (Weinh). 2021;8:2003395 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4e
Reis M, Willis G, Fernandez Gonzalez A, Yeung V, Taglauer E, Magaletta M, et al. Mesenchymal Stromal Cell-Derived Extracellular Vesicles Restore Thymic Architecture and T Cell Function Disrupted by Neonatal Hyperoxia. Front Immunol. 2021;12:640595 pubmed publisher
  • flow cytometry; mouse; 1:200
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; 1:250; loading ...; fig s8b
Torretta S, Scagliola A, Ricci L, Mainini F, Di Marco S, Cuccovillo I, et al. D-mannose suppresses macrophage IL-1β production. Nat Commun. 2020;11:6343 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Manils J, Webb L, Howes A, Janzen J, Boeing S, Bowcock A, et al. CARD14E138A signalling in keratinocytes induces TNF-dependent skin and systemic inflammation. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Bergmann B, Fei Y, Jirholt P, Hu Z, Bergquist M, Ali A, et al. Pre-treatment with IL2 gene therapy alleviates Staphylococcus aureus arthritis in mice. BMC Infect Dis. 2020;20:185 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6a
Sugiura D, Maruhashi T, Okazaki I, Shimizu K, Maeda T, Takemoto T, et al. Restriction of PD-1 function by cis-PD-L1/CD80 interactions is required for optimal T cell responses. Science. 2019;364:558-566 pubmed publisher
  • flow cytometry; mouse; fig 1a
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Qian L, Bajana S, Georgescu C, Peng V, Wang H, Adrianto I, et al. Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors. J Exp Med. 2019;216:884-899 pubmed publisher
  • flow cytometry; mouse; fig 4f
Silva D, Yu S, Ulge U, Spangler J, Jude K, Labao Almeida C, et al. De novo design of potent and selective mimics of IL-2 and IL-15. Nature. 2019;565:186-191 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; fig s13b
Kornberg M, Bhargava P, Kim P, Putluri V, Snowman A, Putluri N, et al. Dimethyl fumarate targets GAPDH and aerobic glycolysis to modulate immunity. Science. 2018;360:449-453 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s4a
Yeh C, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018;9:928 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 8
Kornete M, Marone R, Jeker L. Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells. J Immunol. 2018;200:2489-2501 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1j
Lynch J, Werder R, Loh Z, Sikder M, Curren B, Zhang V, et al. Plasmacytoid dendritic cells protect from viral bronchiolitis and asthma through semaphorin 4a-mediated T reg expansion. J Exp Med. 2018;215:537-557 pubmed publisher
  • flow cytometry; mouse; fig 1a
Taylor S, Huang Y, Mallett G, Stathopoulou C, Felizardo T, Sun M, et al. PD-1 regulates KLRG1+ group 2 innate lymphoid cells. J Exp Med. 2017;214:1663-1678 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
González Pérez G, Lamousé Smith E. Gastrointestinal Microbiome Dysbiosis in Infant Mice Alters Peripheral CD8+ T Cell Receptor Signaling. Front Immunol. 2017;8:265 pubmed publisher
Stump C, Roehle K, Manjarrez Orduno N, Dougan S. Radiation combines with immune checkpoint blockade to enhance T cell priming in a murine model of poorly immunogenic pancreatic cancer. Open Biol. 2021;11:210245 pubmed publisher
Wong H, Park K, Gola A, Baptista A, Miller C, Deep D, et al. A local regulatory T cell feedback circuit maintains immune homeostasis by pruning self-activated T cells. Cell. 2021;184:3981-3997.e22 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Schofield D, Percival Alwyn J, Rytelewski M, Hood J, Rothstein R, Wetzel L, et al. Activity of murine surrogate antibodies for durvalumab and tremelimumab lacking effector function and the ability to deplete regulatory T cells in mouse models of cancer. MAbs. 2021;13:1857100 pubmed publisher
Loh Z, Simpson J, Ullah A, Zhang V, Gan W, Lynch J, et al. HMGB1 amplifies ILC2-induced type-2 inflammation and airway smooth muscle remodelling. PLoS Pathog. 2020;16:e1008651 pubmed publisher
Shakyawar D, Muralikrishna B, Radha V. C3G dynamically associates with nuclear speckles and regulates mRNA splicing. Mol Biol Cell. 2018;: pubmed publisher
Yuan M, Li D, An M, Li Q, Zhang L, Wang G. Rediscovering peritoneal macrophages in a murine endometriosis model. Hum Reprod. 2017;32:94-102 pubmed
Watanabe N, Kaminuma O, Kitamura N, Hiroi T. Induced Treg Cells Augment the Th17-Mediated Intestinal Inflammatory Response in a CTLA4-Dependent Manner. PLoS ONE. 2016;11:e0150244 pubmed publisher
Galitovskiy V, Qian J, Chernyavsky A, Marchenko S, Gindi V, Edwards R, et al. Cytokine-induced alterations of ?7 nicotinic receptor in colonic CD4 T cells mediate dichotomous response to nicotine in murine models of Th1/Th17- versus Th2-mediated colitis. J Immunol. 2011;187:2677-87 pubmed publisher
product information
Antigen :
CD25
Apps. Abbrev. :
Flow Cytometry
Cat # :
102017
Clone :
PC61
Item :
Alexa Fluor® 488 anti-mouse CD25
Isotype :
Rat IgG1, λ
Other Names :
IL-2Rα, Ly-43, p55, Tac
Size :
100 μg
Price (USD) :
215 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A488
Immunogen :
IL-2-dependent cytolytic mouse T-cell clone B6.1
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation 1,2 , in vitro blocking of IL-2 binding to low- and high-affinity receptors 1-4 , growth inhibition of IL-2-dependent T-cell lines 1-4 , in vivo depletion of CD25 + CD4 + Treg cells 5-8,10 , and immunohistochemical staining of acetone-fixed frozen sections 2 . PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 102040) with endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA