product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse CD25
catalog :
102012
quantity :
100 μg
price :
140 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
PC61
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 41
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4c
Manils J, Webb L, Howes A, Janzen J, Boeing S, Bowcock A, et al. CARD14E138A signalling in keratinocytes induces TNF-dependent skin and systemic inflammation. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b, 4c, 4d
Chen C, Chencheng Z, Cuiying L, Xiaokun G. Plasmacytoid Dendritic Cells Protect Against Middle Cerebral Artery Occlusion Induced Brain Injury by Priming Regulatory T Cells. Front Cell Neurosci. 2020;14:8 pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig s1a
Lyons J, Ghazi P, Starchenko A, Tovaglieri A, Baldwin K, Poulin E, et al. The colonic epithelium plays an active role in promoting colitis by shaping the tissue cytokine profile. PLoS Biol. 2018;16:e2002417 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Hu Y, Kim J, He K, Wan Q, Kim J, Flach M, et al. Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion. J Exp Med. 2016;213:2759-2772 pubmed
  • flow cytometry; mouse
Tassi I, Claudio E, Wang H, Tang W, Ha H, Saret S, et al. The NF-κB regulator Bcl-3 governs dendritic cell antigen presentation functions in adaptive immunity. J Immunol. 2014;193:4303-11 pubmed publisher
Kashiwakura Y, Kojima H, Kanno Y, Hashiguchi M, Kobata T. Heparin affects the induction of regulatory T cells independent of anti-coagulant activity and suppresses allogeneic immune responses. Clin Exp Immunol. 2020;202:119-135 pubmed publisher
Jing Y, Dai X, Yang L, Kang D, Jiang P, Li N, et al. STING couples with PI3K to regulate actin reorganization during BCR activation. Sci Adv. 2020;6:eaax9455 pubmed publisher
Kolev M, West E, Kunz N, Chauss D, Moseman E, Rahman J, et al. Diapedesis-Induced Integrin Signaling via LFA-1 Facilitates Tissue Immunity by Inducing Intrinsic Complement C3 Expression in Immune Cells. Immunity. 2020;52:513-527.e8 pubmed publisher
Annu K, Cline C, Yasuda K, Ganguly S, Pesch A, Cooper B, et al. Role of Vitamins A and D in BCR-ABL Arf-/- Acute Lymphoblastic Leukemia. Sci Rep. 2020;10:2359 pubmed publisher
Angelou C, Wells A, Vijayaraghavan J, Dougan C, Lawlor R, Iverson E, et al. Differentiation of Pathogenic Th17 Cells Is Negatively Regulated by Let-7 MicroRNAs in a Mouse Model of Multiple Sclerosis. Front Immunol. 2019;10:3125 pubmed publisher
Kang T, Park J, Shin D, Choi H, Kim J, Lee M. Brown Adipocyte and Splenocyte Co-Culture Maintains Regulatory T Cell Subset in Intermittent Hypobaric Conditions. Tissue Eng Regen Med. 2019;16:539-548 pubmed publisher
Bertino P, Premeaux T, Fujita T, Haun B, Marciel M, Hoffmann F, et al. Targeting the C-terminus of galectin-9 induces mesothelioma apoptosis and M2 macrophage depletion. Oncoimmunology. 2019;8:1601482 pubmed publisher
Schuster M, Plaza Sirvent C, Visekruna A, Huehn J, Schmitz I. Generation of Foxp3+CD25- Regulatory T-Cell Precursors Requires c-Rel and IκBNS. Front Immunol. 2019;10:1583 pubmed publisher
Chen X, Luo Y, Wang M, Sun L, Huang K, Li Y, et al. Wuhu Decoction Regulates Dendritic Cell Autophagy in the Treatment of Respiratory Syncytial Virus (RSV)-Induced Mouse Asthma by AMPK/ULK1 Signaling Pathway. Med Sci Monit. 2019;25:5389-5400 pubmed publisher
Kim J, Lim I, Joo H, Park C, Choi S, Jeong H, et al. Fimasartan reduces neointimal formation and inflammation after carotid arterial injury in apolipoprotein E knockout mice. Mol Med. 2019;25:33 pubmed publisher
Shi L, Sheng J, Wang M, Luo H, Zhu J, Zhang B, et al. Combination Therapy of TGF-β Blockade and Commensal-derived Probiotics Provides Enhanced Antitumor Immune Response and Tumor Suppression. Theranostics. 2019;9:4115-4129 pubmed publisher
Niven J, Madelon N, Page N, Caruso A, Harlé G, Lemeille S, et al. Macroautophagy in Dendritic Cells Controls the Homeostasis and Stability of Regulatory T Cells. Cell Rep. 2019;28:21-29.e6 pubmed publisher
Yue X, Lio C, Samaniego Castruita D, Li X, Rao A. Loss of TET2 and TET3 in regulatory T cells unleashes effector function. Nat Commun. 2019;10:2011 pubmed publisher
Wang B, Lim J, Kajikawa T, Li X, Vallance B, Moutsopoulos N, et al. Macrophage β2-Integrins Regulate IL-22 by ILC3s and Protect from Lethal Citrobacter rodentium-Induced Colitis. Cell Rep. 2019;26:1614-1626.e5 pubmed publisher
Kemp V, van den Wollenberg D, Camps M, van Hall T, Kinderman P, Pronk van Montfoort N, et al. Arming oncolytic reovirus with GM-CSF gene to enhance immunity. Cancer Gene Ther. 2018;: pubmed publisher
Wang T, Fan C, Yao A, Xu X, Zheng G, You Y, et al. The Adaptor Protein CARD9 Protects against Colon Cancer by Restricting Mycobiota-Mediated Expansion of Myeloid-Derived Suppressor Cells. Immunity. 2018;49:504-514.e4 pubmed publisher
Barbet G, Sander L, Geswell M, Leonardi I, Cerutti A, Iliev I, et al. Sensing Microbial Viability through Bacterial RNA Augments T Follicular Helper Cell and Antibody Responses. Immunity. 2018;48:584-598.e5 pubmed publisher
Johnson J, Georgakilas G, Petrovic J, Kurachi M, Cai S, Harly C, et al. Lineage-Determining Transcription Factor TCF-1 Initiates the Epigenetic Identity of T Cells. Immunity. 2018;48:243-257.e10 pubmed publisher
Zimmermann J, Durek P, Kuhl A, Schattenberg F, Maschmeyer P, Siracusa F, et al. The intestinal microbiota determines the colitis-inducing potential of T-bet-deficient Th cells in mice. Eur J Immunol. 2018;48:161-167 pubmed publisher
Pierini A, Iliopoulou B, Peiris H, Pérez Cruz M, Baker J, Hsu K, et al. T cells expressing chimeric antigen receptor promote immune tolerance. JCI Insight. 2017;2: pubmed publisher
Prajeeth C, Kronisch J, Khorooshi R, Knier B, Toft Hansen H, Gudi V, et al. Effectors of Th1 and Th17 cells act on astrocytes and augment their neuroinflammatory properties. J Neuroinflammation. 2017;14:204 pubmed publisher
Hayatsu N, Miyao T, Tachibana M, Murakami R, Kimura A, Kato T, et al. Analyses of a Mutant Foxp3 Allele Reveal BATF as a Critical Transcription Factor in the Differentiation and Accumulation of Tissue Regulatory T Cells. Immunity. 2017;47:268-283.e9 pubmed publisher
Schuster M, Plaza Sirvent C, Matthies A, Heise U, Jeron A, Bruder D, et al. c-REL and I?BNS Govern Common and Independent Steps of Regulatory T Cell Development from Novel CD122-Expressing Pre-Precursors. J Immunol. 2017;199:920-930 pubmed publisher
Pardo E, Cárcamo C, Uribe San Martin R, Ciampi E, Segovia Miranda F, Curkovic Peña C, et al. Galectin-8 as an immunosuppressor in experimental autoimmune encephalomyelitis and a target of human early prognostic antibodies in multiple sclerosis. PLoS ONE. 2017;12:e0177472 pubmed publisher
Nenasheva T, Nikolaev A, Diykanov D, Sukhanova A, Tcyganov E, Panteleev A, et al. The introduction of mesenchymal stromal cells induces different immunological responses in the lungs of healthy and M. tuberculosis infected mice. PLoS ONE. 2017;12:e0178983 pubmed publisher
Long C, Marshall N, Lukomska E, Kashon M, Meade B, Shane H, et al. A Role for Regulatory T Cells in a Murine Model of Epicutaneous Toluene Diisocyanate Sensitization. Toxicol Sci. 2016;152:85-98 pubmed publisher
Tanaka S, Yamamoto K, Yamada K, Furuya K, Uyeno Y. Relationship of Enhanced Butyrate Production by Colonic Butyrate-Producing Bacteria to Immunomodulatory Effects in Normal Mice Fed an Insoluble Fraction of Brassica rapa L. Appl Environ Microbiol. 2016;82:2693-2699 pubmed publisher
Min H, Kim S, Baek S, Woo J, Park J, Cho M, et al. Anthocyanin Extracted from Black Soybean Seed Coats Prevents Autoimmune Arthritis by Suppressing the Development of Th17 Cells and Synthesis of Proinflammatory Cytokines by Such Cells, via Inhibition of NF-κB. PLoS ONE. 2015;10:e0138201 pubmed publisher
Wong E, Soni C, Chan A, Domeier P, Shwetank -, Abraham T, et al. B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance. J Immunol. 2015;194:4130-43 pubmed publisher
Waysbort N, Russ D, Chain B, Friedman N. Coupled IL-2-dependent extracellular feedbacks govern two distinct consecutive phases of CD4 T cell activation. J Immunol. 2013;191:5822-30 pubmed publisher
Horikoshi M, Goto D, Segawa S, Yoshiga Y, Iwanami K, Inoue A, et al. Activation of Invariant NKT cells with glycolipid ligand ?-galactosylceramide ameliorates glucose-6-phosphate isomerase peptide-induced arthritis. PLoS ONE. 2012;7:e51215 pubmed publisher
Oomizu S, Arikawa T, Niki T, Kadowaki T, Ueno M, Nishi N, et al. Galectin-9 suppresses Th17 cell development in an IL-2-dependent but Tim-3-independent manner. Clin Immunol. 2012;143:51-8 pubmed publisher
Kolbus D, Wigren M, Ljungcrantz I, Söderberg I, Alm R, Bjorkbacka H, et al. Immunization with cationized BSA inhibits progression of disease in ApoBec-1/LDL receptor deficient mice with manifest atherosclerosis. Immunobiology. 2011;216:663-9 pubmed publisher
Wigren M, Kolbus D, Duner P, Ljungcrantz I, Söderberg I, Bjorkbacka H, et al. Evidence for a role of regulatory T cells in mediating the atheroprotective effect of apolipoprotein B peptide vaccine. J Intern Med. 2011;269:546-56 pubmed publisher
Ramírez Villegas J, Khoury C, Jarvis A, Debouck D, Guarino L. A gap analysis methodology for collecting crop genepools: a case study with phaseolus beans. PLoS ONE. 2010;5:e13497 pubmed publisher
Noris M, Cassis P, Azzollini N, Cavinato R, Cugini D, Casiraghi F, et al. The Toll-IL-1R member Tir8/SIGIRR negatively regulates adaptive immunity against kidney grafts. J Immunol. 2009;183:4249-60 pubmed publisher
product information
Antigen :
CD25
Apps. Abbrev. :
Flow Cytometry
Cat # :
102012
Clone :
PC61
Item :
APC anti-mouse CD25
Isotype :
Rat IgG1, λ
Other Names :
IL-2Rα, Ly-43, p55, Tac
Size :
100 μg
Price (USD) :
140 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC
Immunogen :
IL-2-dependent cytolytic mouse T-cell clone B6.1
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation 1,2 , in vitro blocking of IL-2 binding to low- and high-affinity receptors 1-4 , growth inhibition of IL-2-dependent T-cell lines 1-4 , in vivo depletion of CD25 + CD4 + Treg cells 5-8,10 , and immunohistochemical staining of acetone-fixed frozen sections 2 . PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA