PE/Cyanine5 anti-mouse CD25 | BioLegend 102010 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine5 anti-mouse CD25

catalog :

102010

quantity :

100 μg

price :

253 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

PC61

reactivity :

mouse

application :

immunohistochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s5e	Lee A, Pingali S, Pinilla Ibarz J, Atchison M, Koumenis C, Argon Y, et al. Loss of AID exacerbates the malignant progression of CLL. Leukemia. 2022;36:2430-2442 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Costain A, Phythian Adams A, Colombo S, Marley A, Owusu C, Cook P, et al. Dynamics of Host Immune Response Development During Schistosoma mansoni Infection. Front Immunol. 2022;13:906338 pubmed publisher
flow cytometry; mouse; fig 3f	Wu B, Song M, Dong Q, Xiang G, Li J, Ma X, et al. UBR5 promotes tumor immune evasion through enhancing IFN-γ-induced PDL1 transcription in triple negative breast cancer. Theranostics. 2022;12:5086-5102 pubmed publisher
flow cytometry; mouse; 1:100; fig 1g	Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
flow cytometry; mouse; loading ...; fig 7b immunohistochemistry; mouse; fig 7a	Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig e1b	Aslam M, Alemdehy M, Kwesi Maliepaard E, Muhaimin F, Caganova M, Pardieck I, et al. Histone methyltransferase DOT1L controls state-specific identity during B cell differentiation. EMBO Rep. 2021;22:e51184 pubmed publisher
	Marangoni F, Zhakyp A, Corsini M, Geels S, Carrizosa E, Thelen M, et al. Expansion of tumor-associated Treg cells upon disruption of a CTLA-4-dependent feedback loop. Cell. 2021;184:3998-4015.e19 pubmed publisher
	Perrot I, Michaud H, Giraudon Paoli M, Augier S, Docquier A, Gros L, et al. Blocking Antibodies Targeting the CD39/CD73 Immunosuppressive Pathway Unleash Immune Responses in Combination Cancer Therapies. Cell Rep. 2019;27:2411-2425.e9 pubmed publisher
	Zongyi Y, Funian Z, Hao L, Xin W, Ying C, Jialin Z, et al. Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio. PLoS ONE. 2017;12:e0189617 pubmed publisher
	Cippà P, Kraus A, Lindenmeyer M, Chen J, Guimezanes A, Bardwell P, et al. Resistance to ABT-737 in activated T lymphocytes: molecular mechanisms and reversibility by inhibition of the calcineurin-NFAT pathway. Cell Death Dis. 2012;3:e299 pubmed publisher

product information

Antigen :

CD25

Apps. Abbrev. :

Flow Cytometry

Cat # :

102010

Clone :

PC61

Item :

PE/Cyanine5 anti-mouse CD25

Isotype :

Rat IgG1, λ

Other Names :

IL-2Rα, Ly-43, p55, Tac

Size :

100 μg

Price (USD) :

253 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PE_CYANINE5

Immunogen :

IL-2-dependent cytolytic mouse T-cell clone B6.1

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation 1,2 , in vitro blocking of IL-2 binding to low- and high-affinity receptors 1-4 , growth inhibition of IL-2-dependent T-cell lines 1-4 , in vivo depletion of CD25 + CD4 + Treg cells 5-8,10 , and immunohistochemical staining of acetone-fixed frozen sections 2 . PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 102040) with endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered.

more info or order :

BioLegend product webpage