product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse CD25
catalog :
102007
quantity :
50 μg
price :
61 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
PC61
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 64
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Gehlsen U, Stary D, Maass M, Riesner K, Musial G, Stern M, et al. Ocular Graft-versus-Host Disease in a Chemotherapy-Based Minor-Mismatch Mouse Model Features Corneal (Lymph-) Angiogenesis. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; 1:200; fig 2h
Celorrio M, Abellanas M, Rhodes J, Goodwin V, Moritz J, Vadivelu S, et al. Gut microbial dysbiosis after traumatic brain injury modulates the immune response and impairs neurogenesis. Acta Neuropathol Commun. 2021;9:40 pubmed publisher
  • flow cytometry; mouse; 1:200
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6a
Sugiura D, Maruhashi T, Okazaki I, Shimizu K, Maeda T, Takemoto T, et al. Restriction of PD-1 function by cis-PD-L1/CD80 interactions is required for optimal T cell responses. Science. 2019;364:558-566 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6a
van der Weyden L, Arends M, Campbell A, Bald T, Wardle Jones H, Griggs N, et al. Genome-wide in vivo screen identifies novel host regulators of metastatic colonization. Nature. 2017;541:233-236 pubmed publisher
  • flow cytometry; mouse; fig 5c
Keil M, Sonner J, Lanz T, Oezen I, Bunse T, Bittner S, et al. General control non-derepressible 2 (GCN2) in T cells controls disease progression of autoimmune neuroinflammation. J Neuroimmunol. 2016;297:117-26 pubmed publisher
  • flow cytometry; mouse; fig s5
Catarinella M, Monestiroli A, Escobar G, Fiocchi A, Tran N, Aiolfi R, et al. IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment. EMBO Mol Med. 2016;8:155-70 pubmed publisher
  • flow cytometry; mouse
Olguín J, Fernández J, Salinas N, Juárez I, Rodriguez Sosa M, Campuzano J, et al. Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated Th1 immune response. Microbes Infect. 2015;17:586-95 pubmed publisher
  • flow cytometry; mouse
White C, Villarino N, Sloan S, Ganusov V, Schmidt N. Plasmodium suppresses expansion of T cell responses to heterologous infections. J Immunol. 2015;194:697-708 pubmed publisher
  • flow cytometry; mouse
Keswani T, Bhattacharyya A. Differential role of T regulatory and Th17 in Swiss mice infected with Plasmodium berghei ANKA and Plasmodium yoelii. Exp Parasitol. 2014;141:82-92 pubmed publisher
Chen Y, Liu R, Cui Y, Hettinghouse A, Fu W, Zhang L, et al. Penfluridol targets acid sphingomyelinase to inhibit TNF signaling and is therapeutic against inflammatory autoimmune diseases. Arthritis Res Ther. 2022;24:27 pubmed publisher
Zhai X, Liu K, Fang H, Zhang Q, Gao X, Liu F, et al. Mitochondrial C1qbp promotes differentiation of effector CD8+ T cells via metabolic-epigenetic reprogramming. Sci Adv. 2021;7:eabk0490 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Sun Y, Preiss N, Valenteros K, Kamal Y, Usherwood Y, Frost H, et al. Zbtb20 Restrains CD8 T Cell Immunometabolism and Restricts Memory Differentiation and Antitumor Immunity. J Immunol. 2020;205:2649-2666 pubmed publisher
Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, et al. CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model. Front Immunol. 2020;11:1883 pubmed publisher
Díaz Salazar C, Sun J. Coordinated Viral Control by Cytotoxic Lymphocytes Ensures Optimal Adaptive NK Cell Responses. Cell Rep. 2020;32:108186 pubmed publisher
Imanishi T, Unno M, Kobayashi W, Yoneda N, Akira S, Saito T. mTORC1 Signaling Controls TLR2-Mediated T-Cell Activation by Inducing TIRAP Expression. Cell Rep. 2020;32:107911 pubmed publisher
Liu Y, Tang B, Wang F, Tang L, Lei Y, Luo Y, et al. Parthenolide ameliorates colon inflammation through regulating Treg/Th17 balance in a gut microbiota-dependent manner. Theranostics. 2020;10:5225-5241 pubmed publisher
Wen J, Wang Y, Valentine K, Dos Santos Alves R, Xu Z, Regla Nava J, et al. CD4+ T Cells Cross-Reactive with Dengue and Zika Viruses Protect against Zika Virus Infection. Cell Rep. 2020;31:107566 pubmed publisher
Tong D, Zhang L, Ning F, Xu Y, Hu X, Shi Y. Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival. Protein Cell. 2020;11:108-123 pubmed publisher
Sendler M, van den Brandt C, Glaubitz J, Wilden A, Golchert J, Weiss F, et al. NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis. Gastroenterology. 2020;158:253-269.e14 pubmed publisher
Elong Ngono A, Shresta S. Cross-Reactive T Cell Immunity to Dengue and Zika Viruses: New Insights Into Vaccine Development. Front Immunol. 2019;10:1316 pubmed publisher
Yang B, Wang K, Wan S, Liang Y, Yuan X, Dong Y, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27:3629-3645.e6 pubmed publisher
Timilshina M, You Z, Lacher S, Acharya S, Jiang L, Kang Y, et al. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep. 2019;27:2948-2961.e7 pubmed publisher
Zhou J, Huang S, Wang Z, Huang J, Xu L, Tang X, et al. Targeting EZH2 histone methyltransferase activity alleviates experimental intestinal inflammation. Nat Commun. 2019;10:2427 pubmed publisher
Schoeler K, Aufschnaiter A, Messner S, Derudder E, Herzog S, Villunger A, et al. TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells. FEBS J. 2019;286:3566-3581 pubmed publisher
Baptista A, Gola A, Huang Y, Milanez Almeida P, Torabi Parizi P, Urban J, et al. The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4+ T Cell Peripheralization to Promote Effective Adaptive Immunity. Immunity. 2019;50:1188-1201.e6 pubmed publisher
Dahlgren M, Jones S, Cautivo K, Dubinin A, Ortiz Carpena J, Farhat S, et al. Adventitial Stromal Cells Define Group 2 Innate Lymphoid Cell Tissue Niches. Immunity. 2019;50:707-722.e6 pubmed publisher
Britton G, Contijoch E, Mogno I, Vennaro O, Llewellyn S, Ng R, et al. Microbiotas from Humans with Inflammatory Bowel Disease Alter the Balance of Gut Th17 and RORγt+ Regulatory T Cells and Exacerbate Colitis in Mice. Immunity. 2019;50:212-224.e4 pubmed publisher
Jayachandran R, Gumienny A, Bolinger B, Ruehl S, Lang M, Fucile G, et al. Disruption of Coronin 1 Signaling in T Cells Promotes Allograft Tolerance while Maintaining Anti-Pathogen Immunity. Immunity. 2019;50:152-165.e8 pubmed publisher
Cantor D, King B, Blumenberg L, Dimauro T, Aifantis I, Koralov S, et al. Impaired Expression of Rearranged Immunoglobulin Genes and Premature p53 Activation Block B Cell Development in BMI1 Null Mice. Cell Rep. 2019;26:108-118.e4 pubmed publisher
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
Becher J, Simula L, Volpe E, Procaccini C, La Rocca C, D Acunzo P, et al. AMBRA1 Controls Regulatory T-Cell Differentiation and Homeostasis Upstream of the FOXO3-FOXP3 Axis. Dev Cell. 2018;47:592-607.e6 pubmed publisher
Tyagi A, Yu M, Darby T, Vaccaro C, Li J, Owens J, et al. The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. Immunity. 2018;49:1116-1131.e7 pubmed publisher
O Malley G, Treacy O, Lynch K, Naicker S, Leonard N, Lohan P, et al. Stromal Cell PD-L1 Inhibits CD8+ T-cell Antitumor Immune Responses and Promotes Colon Cancer. Cancer Immunol Res. 2018;6:1426-1441 pubmed publisher
Draijer C, Robbe P, Boorsma C, Hylkema M, Melgert B. Dual role of YM1+ M2 macrophages in allergic lung inflammation. Sci Rep. 2018;8:5105 pubmed publisher
Li Z, Gothard E, Coles M, Ambler C. Quantitative Methods for Measuring Repair Rates and Innate-Immune Cell Responses in Wounded Mouse Skin. Front Immunol. 2018;9:347 pubmed publisher
Hsieh W, Hsu T, Chang Y, Lai M. IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells. Nat Commun. 2018;9:463 pubmed publisher
Yuan X, Yang B, Dong Y, Yamamura A, Fu W. CRIg, a tissue-resident macrophage specific immune checkpoint molecule, promotes immunological tolerance in NOD mice, via a dual role in effector and regulatory T cells. elife. 2017;6: pubmed publisher
Schüler F, Weiss J, Lindner S, Lohmüller M, Herzog S, Spiegl S, et al. Checkpoint kinase 1 is essential for normal B cell development and lymphomagenesis. Nat Commun. 2017;8:1697 pubmed publisher
Ma W, Liu Q, Yang J, Yang Y, Zhao Z, Ma H, et al. A Mouse Model of Autoimmune Cholangitis via Syngeneic Bile Duct Protein Immunization. Sci Rep. 2017;7:15246 pubmed publisher
Tanaka Y, Sotome T, Inoue A, Mukozu T, Kuwabara T, Mikami T, et al. SATB1 Conditional Knockout Results in Sjögren's Syndrome in Mice. J Immunol. 2017;199:4016-4022 pubmed publisher
Xu L, Huang Q, Wang H, Hao Y, Bai Q, Hu J, et al. The Kinase mTORC1 Promotes the Generation and Suppressive Function of Follicular Regulatory T Cells. Immunity. 2017;47:538-551.e5 pubmed publisher
Verma K, Jyotsana N, Buenting I, Luther S, Pfanne A, Thum T, et al. miR-625-3p is upregulated in CD8+ T cells during early immune reconstitution after allogeneic stem cell transplantation. PLoS ONE. 2017;12:e0183828 pubmed publisher
Wells A, Daniels K, Angelou C, Fagerberg E, Burnside A, Markstein M, et al. Modulation of let-7 miRNAs controls the differentiation of effector CD8 T cells. elife. 2017;6: pubmed publisher
Timilshina M, Kang Y, Dahal I, You Z, Nam T, Kim K, et al. BJ-3105, a 6-Alkoxypyridin-3-ol Analog, Impairs T Cell Differentiation and Prevents Experimental Autoimmune Encephalomyelitis Disease Progression. PLoS ONE. 2017;12:e0168942 pubmed publisher
Inomata T, Hua J, Di Zazzo A, Dana R. Impaired Function of Peripherally Induced Regulatory T Cells in Hosts at High Risk of Graft Rejection. Sci Rep. 2016;6:39924 pubmed publisher
Galle Treger L, Suzuki Y, Patel N, Sankaranarayanan I, Aron J, Maazi H, et al. Nicotinic acetylcholine receptor agonist attenuates ILC2-dependent airway hyperreactivity. Nat Commun. 2016;7:13202 pubmed publisher
Biton J, Khaleghparast Athari S, Thiolat A, Santinon F, Lemeiter D, Hervé R, et al. In Vivo Expansion of Activated Foxp3+ Regulatory T Cells and Establishment of a Type 2 Immune Response upon IL-33 Treatment Protect against Experimental Arthritis. J Immunol. 2016;197:1708-19 pubmed publisher
Chou T, Chuang Y, Hsieh W, Chang P, Liu H, Mo S, et al. Tumour suppressor death-associated protein kinase targets cytoplasmic HIF-1? for Th17 suppression. Nat Commun. 2016;7:11904 pubmed publisher
Orlanski S, Labi V, Reizel Y, Spiro A, Lichtenstein M, Levin Klein R, et al. Tissue-specific DNA demethylation is required for proper B-cell differentiation and function. Proc Natl Acad Sci U S A. 2016;113:5018-23 pubmed publisher
Guo Z, Kong Q, Liu C, Zhang S, Zou L, Yan F, et al. DCAF1 controls T-cell function via p53-dependent and -independent mechanisms. Nat Commun. 2016;7:10307 pubmed publisher
Kondo M, Tanaka Y, Kuwabara T, Naito T, Kohwi Shigematsu T, Watanabe A. SATB1 Plays a Critical Role in Establishment of Immune Tolerance. J Immunol. 2016;196:563-72 pubmed publisher
Holvoet B, Quattrocelli M, Belderbos S, Pollaris L, Wolfs E, Gheysens O, et al. Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice. Stem Cell Reports. 2015;5:1183-1195 pubmed publisher
Kim B, Sasaki Y, Egawa T. Restriction of Nonpermissive RUNX3 Protein Expression in T Lymphocytes by the Kozak Sequence. J Immunol. 2015;195:1517-23 pubmed publisher
Ibrahim M, Widjajanto E, Widodo M, Sumitro S. EMSA Eritin Drives Expansion of Regulatory T Cells and Promotes T Cells Differentiation in Irradiated Mice. J Evid Based Complementary Altern Med. 2016;21:171-6 pubmed publisher
Flaherty S, Reynolds J. Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets. J Vis Exp. 2015;: pubmed publisher
Charlton J, Tsoukatou D, Mamalaki C, Chatzidakis I. Programmed death 1 regulates memory phenotype CD4 T cell accumulation, inhibits expansion of the effector memory phenotype subset and modulates production of effector cytokines. PLoS ONE. 2015;10:e0119200 pubmed publisher
Singh K, Hjort M, Thorvaldson L, Sandler S. Concomitant analysis of Helios and Neuropilin-1 as a marker to detect thymic derived regulatory T cells in naïve mice. Sci Rep. 2015;5:7767 pubmed publisher
Lu X, Ding Z, Cao Y, Liu C, Habtetsion T, Yu M, et al. Alkylating agent melphalan augments the efficacy of adoptive immunotherapy using tumor-specific CD4+ T cells. J Immunol. 2015;194:2011-21 pubmed publisher
Madireddi S, Eun S, Lee S, Nemčovičová I, Mehta A, Zajonc D, et al. Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies. J Exp Med. 2014;211:1433-48 pubmed publisher
Merck E, Lees R, Voyle R, Held W, MacDonald H. Ly49D-mediated ITAM signaling in immature thymocytes impairs development by bypassing the pre-TCR checkpoint. J Immunol. 2011;187:110-7 pubmed publisher
product information
Antigen :
CD25
Apps. Abbrev. :
Flow Cytometry
Cat # :
102007
Clone :
PC61
Item :
PE anti-mouse CD25
Isotype :
Rat IgG1, λ
Other Names :
IL-2Rα, Ly-43, p55, Tac
Size :
50 μg
Price (USD) :
61 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE
Immunogen :
IL-2-dependent cytolytic mouse T-cell clone B6.1
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation 1,2 , in vitro blocking of IL-2 binding to low- and high-affinity receptors 1-4 , growth inhibition of IL-2-dependent T-cell lines 1-4 , in vivo depletion of CD25 + CD4 + Treg cells 5-8,10 , and immunohistochemical staining of acetone-fixed frozen sections 2 . PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 102040) with endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA