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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-mouse CD25
catalog :
102005
quantity :
50 μg
price :
66 USD
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
PC61
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 26
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig s1b
Pereira J, Cavaco P, da Silva R, Pacheco Leyva I, Mereiter S, Pinto R, et al. P-selectin glycoprotein ligand 1 promotes T cell lymphoma development and dissemination. Transl Oncol. 2021;14:101125 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4e
Reis M, Willis G, Fernandez Gonzalez A, Yeung V, Taglauer E, Magaletta M, et al. Mesenchymal Stromal Cell-Derived Extracellular Vesicles Restore Thymic Architecture and T Cell Function Disrupted by Neonatal Hyperoxia. Front Immunol. 2021;12:640595 pubmed publisher
  • flow cytometry; mouse; loading ...
Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
Otano I, Azpilikueta A, Glez Vaz J, Alvarez M, Medina Echeverz J, Cortés Domínguez I, et al. CD137 (4-1BB) costimulation of CD8+ T cells is more potent when provided in cis than in trans with respect to CD3-TCR stimulation. Nat Commun. 2021;12:7296 pubmed publisher
Boccasavia V, Bovolenta E, Villanueva A, Borroto A, Oeste C, van Santen H, et al. Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen. Cell Rep. 2021;34:108861 pubmed publisher
Harsha Krovi S, Zhang J, Michaels Foster M, Brunetti T, Loh L, Scott Browne J, et al. Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells. Nat Commun. 2020;11:6238 pubmed publisher
Moon J, Lee S, Lee S, Ryu J, Jhun J, Choi J, et al. GRIM-19 Ameliorates Multiple Sclerosis in a Mouse Model of Experimental Autoimmune Encephalomyelitis with Reciprocal Regulation of IFNγ/Th1 and IL-17A/Th17 Cells. Immune Netw. 2020;20:e40 pubmed publisher
Kashiwakura Y, Kojima H, Kanno Y, Hashiguchi M, Kobata T. Heparin affects the induction of regulatory T cells independent of anti-coagulant activity and suppresses allogeneic immune responses. Clin Exp Immunol. 2020;202:119-135 pubmed publisher
Sendler M, van den Brandt C, Glaubitz J, Wilden A, Golchert J, Weiss F, et al. NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis. Gastroenterology. 2020;158:253-269.e14 pubmed publisher
Etxeberria I, Bolaños E, Quetglas J, Gros A, Villanueva A, Palomero J, et al. Intratumor Adoptive Transfer of IL-12 mRNA Transiently Engineered Antitumor CD8+ T Cells. Cancer Cell. 2019;36:613-629.e7 pubmed publisher
Sasaki K, Himeno A, Nakagawa T, Sasaki Y, Kiyonari H, Iwai K. Modulation of autoimmune pathogenesis by T cell-triggered inflammatory cell death. Nat Commun. 2019;10:3878 pubmed publisher
Schuster M, Plaza Sirvent C, Visekruna A, Huehn J, Schmitz I. Generation of Foxp3+CD25- Regulatory T-Cell Precursors Requires c-Rel and IκBNS. Front Immunol. 2019;10:1583 pubmed publisher
Celis Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Gregoire C, et al. Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Cell Rep. 2019;27:3315-3330.e7 pubmed publisher
Kyburz A, Fallegger A, Zhang X, Altobelli A, Artola Borán M, Borbet T, et al. Transmaternal Helicobacter pylori exposure reduces allergic airway inflammation in offspring through regulatory T cells. J Allergy Clin Immunol. 2019;143:1496-1512.e11 pubmed publisher
Wan X, Zinselmeyer B, Zakharov P, Vomund A, Taniguchi R, Santambrogio L, et al. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Nature. 2018;560:107-111 pubmed publisher
Amantini C, Farfariello V, Cardinali C, Morelli M, Marinelli O, Nabissi M, et al. The TRPV1 ion channel regulates thymocyte differentiation by modulating autophagy and proteasome activity. Oncotarget. 2017;8:90766-90780 pubmed publisher
Schuster M, Plaza Sirvent C, Matthies A, Heise U, Jeron A, Bruder D, et al. c-REL and I?BNS Govern Common and Independent Steps of Regulatory T Cell Development from Novel CD122-Expressing Pre-Precursors. J Immunol. 2017;199:920-930 pubmed publisher
Mier Aguilar C, Cashman K, Raman C, Soldevila G. CD5-CK2 Signaling Modulates Erk Activation and Thymocyte Survival. PLoS ONE. 2016;11:e0168155 pubmed publisher
Qi S, Li H, Lu L, Qi Z, Liu L, Chen L, et al. Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy. elife. 2016;5: pubmed publisher
Cerina M, Narayanan V, Göbel K, Bittner S, Ruck T, Meuth P, et al. The quality of cortical network function recovery depends on localization and degree of axonal demyelination. Brain Behav Immun. 2017;59:103-117 pubmed publisher
Kang J, Lee J, Chang J. Cholera Toxin Promotes Th17 Cell Differentiation by Modulating Expression of Polarizing Cytokines and the Antigen-Presenting Potential of Dendritic Cells. PLoS ONE. 2016;11:e0157015 pubmed publisher
Thornton T, Delgado P, Chen L, Salas B, Krementsov D, Fernández M, et al. Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response. Nat Commun. 2016;7:10553 pubmed publisher
Naik A, Hawwari A, Krangel M. Specification of Vδ and Vα usage by Tcra/Tcrd locus V gene segment promoters. J Immunol. 2015;194:790-4 pubmed publisher
Xia S, Wei J, Wang J, Sun H, Zheng W, Li Y, et al. A requirement of dendritic cell-derived interleukin-27 for the tumor infiltration of regulatory T cells. J Leukoc Biol. 2014;95:733-742 pubmed
Carbonnelle D, Duflos M, Marchand P, Chauvet C, Petit J, Lang F. A novel indole-3-propanamide exerts its immunosuppressive activity by inhibiting JAK3 in T cells. J Pharmacol Exp Ther. 2009;331:710-6 pubmed publisher
Toubaji A, Hill S, Terabe M, Qian J, Floyd T, Simpson R, et al. The combination of GM-CSF and IL-2 as local adjuvant shows synergy in enhancing peptide vaccines and provides long term tumor protection. Vaccine. 2007;25:5882-91 pubmed
product information
Antigen :
CD25
Apps. Abbrev. :
Flow Cytometry
Cat # :
102005
Clone :
PC61
Item :
FITC anti-mouse CD25
Isotype :
Rat IgG1, λ
Other Names :
IL-2Rα, Ly-43, p55, Tac
Size :
50 μg
Price (USD) :
66 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
FITC
Immunogen :
IL-2-dependent cytolytic mouse T-cell clone B6.1
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation 1,2 , in vitro blocking of IL-2 binding to low- and high-affinity receptors 1-4 , growth inhibition of IL-2-dependent T-cell lines 1-4 , in vivo depletion of CD25 + CD4 + Treg cells 5-8,10 , and immunohistochemical staining of acetone-fixed frozen sections 2 . PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 102040) with endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA