Biotin anti-mouse CD25 | BioLegend 102003 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Biotin anti-mouse CD25

catalog :

102003

quantity :

50 μg

price :

65 USD

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

PC61

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 10

Reference
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Deerhake M, Danzaki K, Inoue M, Cardakli E, Nonaka T, Aggarwal N, et al. Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M. Immunity. 2021;54:484-498.e8 pubmed publisher
Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, et al. PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2. J Cell Biol. 2020;219: pubmed publisher
Frohner I, Mudrak I, Schüchner S, Anrather D, Hartl M, Sontag J, et al. PP2AC Phospho-Tyr307 Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2AC Modifications Including Leu309 Methylation. Cell Rep. 2020;30:3171-3182.e6 pubmed publisher
Yang B, Wang K, Wan S, Liang Y, Yuan X, Dong Y, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27:3629-3645.e6 pubmed publisher
Martínez López M, Iborra S, Conde Garrosa R, Mastrangelo A, Danne C, Mann E, et al. Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity. Immunity. 2019;50:446-461.e9 pubmed publisher
Vural A, Nabar N, Hwang I, Sohn S, Park C, Karlsson M, et al. Gαi2 Signaling Regulates Inflammasome Priming and Cytokine Production by Biasing Macrophage Phenotype Determination. J Immunol. 2019;202:1510-1520 pubmed publisher
Akkaya B, Holstein A, Isaac C, Maz M, Glass D, Shevach E, et al. Ex-vivo iTreg differentiation revisited: Convenient alternatives to existing strategies. J Immunol Methods. 2017;441:67-71 pubmed publisher
Miyauchi K, Sugimoto Ishige A, Harada Y, Adachi Y, Usami Y, Kaji T, et al. Protective neutralizing influenza antibody response in the absence of T follicular helper cells. Nat Immunol. 2016;17:1447-1458 pubmed publisher
Fukushima A, Yamaguchi T, Ishida W, Fukata K, Ueno H. TLR2 agonist ameliorates murine experimental allergic conjunctivitis by inducing CD4 positive T-cell apoptosis rather than by affecting the Th1/Th2 balance. Biochem Biophys Res Commun. 2006;339:1048-55 pubmed

product information

Antigen :

CD25

Apps. Abbrev. :

Flow Cytometry

Cat # :

102003

Clone :

PC61

Item :

Biotin anti-mouse CD25

Isotype :

Rat IgG1, λ

Other Names :

IL-2Rα, Ly-43, p55, Tac

Size :

50 μg

Price (USD) :

65 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BIOTIN

Immunogen :

IL-2-dependent cytolytic mouse T-cell clone B6.1

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation 1,2 , in vitro blocking of IL-2 binding to low- and high-affinity receptors 1-4 , growth inhibition of IL-2-dependent T-cell lines 1-4 , in vivo depletion of CD25 + CD4 + Treg cells 5-8,10 , and immunohistochemical staining of acetone-fixed frozen sections 2 . PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 102040) with endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered.

more info or order :

BioLegend product webpage