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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-mouse CD16/32
catalog :
101342
quantity :
100 mg
price :
3383 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
93
reactivity :
mouse
application :
neutralization, immunoprecipitation, flow cytometry
more info or order :
citations: 25
Reference
Matsudaira T, Nakano S, Konishi Y, Kawamoto S, Uemura K, Kondo T, et al. Cellular senescence in white matter microglia is induced during ageing in mice and exacerbates the neuroinflammatory phenotype. Commun Biol. 2023;6:665 pubmed publisher
van Os B, Kusters P, den Toom M, Beckers L, Van Tiel C, Vos W, et al. Deficiency of germinal center kinase TRAF2 and NCK-interacting kinase (TNIK) in B cells does not affect atherosclerosis. Front Cardiovasc Med. 2023;10:1171764 pubmed publisher
van Os B, Vos W, Bosmans L, Van Tiel C, Lith S, den Toom M, et al. Hyperlipidaemia elicits an atypical, T helper 1-like CD4+ T-cell response: a key role for very low-density lipoprotein. Eur Heart J Open. 2023;3:oead013 pubmed publisher
Yang L, Guo P, Wang P, Wang W, Liu J. IL-6/ERK signaling pathway participates in type I IFN-programmed, unconventional M2-like macrophage polarization. Sci Rep. 2023;13:1827 pubmed publisher
Kraemer A, Sch xe4 fer A, Sprenger D, Sehnert B, Williams J, Luo A, et al. Impact of dietary vitamin D on immunoregulation and disease pathology in lupus-prone NZB/W F1 mice. Front Immunol. 2022;13:933191 pubmed publisher
Miranda K, Becker W, Busbee P, Dopkins N, Abdulla O, Zhong Y, et al. Yin and yang of cannabinoid CB1 receptor: CB1 deletion in immune cells causes exacerbation while deletion in non-immune cells attenuates obesity. iScience. 2022;25:104994 pubmed publisher
Li D, Sun T, Tao L, Ji W, Zhu P, Liang R, et al. A mouse-adapted CVA6 strain exhibits neurotropism and triggers systemic manifestations in a novel murine model. Emerg Microbes Infect. 2022;11:2248-2263 pubmed publisher
Pfenninger P, Yerly L, Abe J. Naïve Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering. Front Immunol. 2022;13:777113 pubmed publisher
Trindade B, Ceglia S, Berthelette A, Raso F, Howley K, Muppidi J, et al. The cholesterol metabolite 25-hydroxycholesterol restrains the transcriptional regulator SREBP2 and limits intestinal IgA plasma cell differentiation. Immunity. 2021;54:2273-2287.e6 pubmed publisher
Schäfer A, Eichhorst A, Hentze C, Kraemer A, Amend A, Sprenger D, et al. Low Dietary Fiber Intake Links Development of Obesity and Lupus Pathogenesis. Front Immunol. 2021;12:696810 pubmed publisher
Guo P, Yang L, Zhang M, Zhang Y, Tong Y, Cao Y, et al. A Monocyte-Orchestrated IFN-I-to-IL-4 Cytokine Axis Instigates Protumoral Macrophages and Thwarts Poly(I:C) Therapy. J Immunol. 2021;207:408-420 pubmed publisher
Liang Y, Sun X, Wang M, Lu Q, Gu M, Zhou L, et al. PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production. Cell Death Differ. 2021;28:2728-2744 pubmed publisher
Amend A, Wickli N, Schäfer A, Sprenger D, Manz R, Voll R, et al. Dual Role of Interleukin-10 in Murine NZB/W F1 Lupus. Int J Mol Sci. 2021;22: pubmed publisher
Nair N, Gomes Solecki M. A Mouse Model of Sublethal Leptospirosis: Protocols for Infection with Leptospira Through Natural Transmission Routes, for Monitoring Clinical and Molecular Scores of Disease, and for Evaluation of the Host Immune Response. Curr Protoc Microbiol. 2020;59:e127 pubmed publisher
Bartleson J, Viehmann Milam A, Donermeyer D, Horvath S, Xia Y, Egawa T, et al. Strength of tonic T cell receptor signaling instructs T follicular helper cell-fate decisions. Nat Immunol. 2020;21:1384-1396 pubmed publisher
Arensman M, Yang X, Zhong W, Bisulco S, Upeslacis E, Rosfjord E, et al. Anti-tumor immunity influences cancer cell reliance upon ATG7. Oncoimmunology. 2020;9:1800162 pubmed publisher
Fox A, Dutt T, Karger B, Obregón Henao A, Anderson G, Henao Tamayo M. Acquisition of High-Quality Spectral Flow Cytometry Data. Curr Protoc Cytom. 2020;93:e74 pubmed publisher
Lercher A, Bhattacharya A, Popa A, Caldera M, Schlapansky M, Baazim H, et al. Type I Interferon Signaling Disrupts the Hepatic Urea Cycle and Alters Systemic Metabolism to Suppress T Cell Function. Immunity. 2019;51:1074-1087.e9 pubmed publisher
Xie Y, Peng J, Pang J, Guo K, Zhang L, Yin S, et al. Biglycan regulates neuroinflammation by promoting M1 microglial activation in early brain injury after experimental subarachnoid hemorrhage. J Neurochem. 2020;152:368-380 pubmed publisher
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product information
Antigen :
CD16/32
Apps. Abbrev. :
FC, IP, Block
Cat # :
101342
Clone :
93
Item :
Ultra-LEAF™ Purified anti-mouse CD16/32
Isotype :
Rat IgG2a, λ
Other Names :
Fcγ R III/II, Ly-17
Size :
100 mg
Price (USD) :
3383 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
ULEAF
Immunogen :
Sorted pre-B cells
Application Notes :
Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation^1 and blocking of Fc-mediated reactions in functional studies^2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 10^6 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA