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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 711™ anti-mouse CD16/32
catalog :
101337
quantity :
50 μg
price :
286 USD
clonality :
monoclonal
host :
rat
conjugate :
BV711
clone name :
93
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 29
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:400; loading ...; fig 3, 4
Aslan K, Turco V, Blobner J, Sonner J, Liuzzi A, Núñez N, et al. Heterogeneity of response to immune checkpoint blockade in hypermutated experimental gliomas. Nat Commun. 2020;11:931 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig ex6i
Pijuan Sala B, Griffiths J, Guibentif C, Hiscock T, Jawaid W, Calero Nieto F, et al. A single-cell molecular map of mouse gastrulation and early organogenesis. Nature. 2019;566:490-495 pubmed publisher
Chavez J, Rabe J, Hernandez G, Mills T, Niño K, Davizon Castillo P, et al. PU.1 Expression Defines Distinct Functional Activities in the Phenotypic HSC Compartment of a Murine Inflammatory Stress Model. Cells. 2022;11: pubmed publisher
Heyde A, Rohde D, McAlpine C, Zhang S, Hoyer F, Gerold J, et al. Increased stem cell proliferation in atherosclerosis accelerates clonal hematopoiesis. Cell. 2021;184:1348-1361.e22 pubmed publisher
Chen R, Okeyo Owuor T, Patel R, Casey E, Cluster A, Yang W, et al. Kmt2c mutations enhance HSC self-renewal capacity and convey a selective advantage after chemotherapy. Cell Rep. 2021;34:108751 pubmed publisher
LeBlond N, Ghorbani P, O Dwyer C, Ambursley N, Nunes J, Smith T, et al. Myeloid deletion and therapeutic activation of AMPK do not alter atherosclerosis in male or female mice. J Lipid Res. 2020;61:1697-1706 pubmed publisher
Li Y, Kong W, Yang W, Patel R, Casey E, Okeyo Owuor T, et al. Single-Cell Analysis of Neonatal HSC Ontogeny Reveals Gradual and Uncoordinated Transcriptional Reprogramming that Begins before Birth. Cell Stem Cell. 2020;27:732-747.e7 pubmed publisher
Pessoa Rodrigues C, Herman J, Herquel B, Valsecchi C, Stehle T, Grün D, et al. Temporal expression of MOF acetyltransferase primes transcription factor networks for erythroid fate. Sci Adv. 2020;6:eaaz4815 pubmed publisher
Frodermann V, Rohde D, Courties G, Severe N, Schloss M, Amatullah H, et al. Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells. Nat Med. 2019;25:1761-1771 pubmed publisher
Liu Q, Chen L, Atkinson J, Claxton D, Wang H. Atg5-dependent autophagy contributes to the development of acute myeloid leukemia in an MLL-AF9-driven mouse model. Cell Death Dis. 2016;7:e2361 pubmed publisher
Kim K, Kim N, Kim S, Kim I, Kim K, Lee G. Cyclo(Phe-Pro) produced by the human pathogen Vibrio vulnificus inhibits host innate immune responses through the NF-κB pathway. Infect Immun. 2015;83:1150-61 pubmed publisher
Melero I, Quetglas J, Reboredo M, Dubrot J, Rodriguez Madoz J, Mancheño U, et al. Strict requirement for vector-induced type I interferon in efficacious antitumor responses to virally encoded IL12. Cancer Res. 2015;75:497-507 pubmed publisher
Everard A, Geurts L, Caesar R, Van Hul M, Matamoros S, Duparc T, et al. Intestinal epithelial MyD88 is a sensor switching host metabolism towards obesity according to nutritional status. Nat Commun. 2014;5:5648 pubmed publisher
Kimura T, Kobayashi S, Hanihara Tatsuzawa F, Sayama A, Maruyama T, Muta T. Responses of macrophages to the danger signals released from necrotic cells. Int Immunol. 2014;26:697-704 pubmed publisher
Cekanaviciute E, Dietrich H, Axtell R, Williams A, Egusquiza R, Wai K, et al. Astrocytic TGF-? signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection. J Immunol. 2014;193:139-49 pubmed publisher
Richardson M, Fu C, Pennington L, Honeycutt J, Odegaard J, Odegaard J, et al. A new mouse model for female genital schistosomiasis. PLoS Negl Trop Dis. 2014;8:e2825 pubmed publisher
Takei S, Omoto C, Kitagawa K, Morishita N, Katayama T, Shigemura K, et al. Oral administration of genetically modified Bifidobacterium displaying HCV-NS3 multi-epitope fusion protein could induce an HCV-NS3-specific systemic immune response in mice. Vaccine. 2014;32:3066-74 pubmed publisher
Fan Y, Mao R, Yu Y, Liu S, Shi Z, Cheng J, et al. USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase. J Exp Med. 2014;211:313-28 pubmed publisher
Huang H, Pan C, Chan Y, Chen J, Wu C. Use of the antimicrobial peptide pardaxin (GE33) to protect against methicillin-resistant Staphylococcus aureus infection in mice with skin injuries. Antimicrob Agents Chemother. 2014;58:1538-45 pubmed publisher
Dutow P, Fehlhaber B, Bode J, Laudeley R, Rheinheimer C, Glage S, et al. The complement C3a receptor is critical in defense against Chlamydia psittaci in mouse lung infection and required for antibody and optimal T cell response. J Infect Dis. 2014;209:1269-78 pubmed publisher
Hegde V, Tomar S, Jackson A, Rao R, Yang X, Singh U, et al. Distinct microRNA expression profile and targeted biological pathways in functional myeloid-derived suppressor cells induced by ?9-tetrahydrocannabinol in vivo: regulation of CCAAT/enhancer-binding protein ? by microRNA-690. J Biol Chem. 2013;288:36810-26 pubmed publisher
Yamazaki S, Maruyama A, Okada K, Matsumoto M, Morita A, Seya T. Dendritic cells from oral cavity induce Foxp3(+) regulatory T cells upon antigen stimulation. PLoS ONE. 2012;7:e51665 pubmed publisher
Huang J, Li X, Kohno K, Hatano M, Tokuhisa T, Murray P, et al. Generation of tissue-specific H-2Kd transgenic mice for the study of K(d)-restricted malaria epitope-specific CD8+ T-cell responses in vivo. J Immunol Methods. 2013;387:254-61 pubmed publisher
Koon H, Ho S, Cheng M, Ichikawa R, Pothoulakis C. Differentiating functional roles of gene expression from immune and non-immune cells in mouse colitis by bone marrow transplantation. J Vis Exp. 2012;:e4208 pubmed publisher
Azuma M, Ebihara T, Oshiumi H, Matsumoto M, Seya T. Cross-priming for antitumor CTL induced by soluble Ag + polyI:C depends on the TICAM-1 pathway in mouse CD11c(+)/CD8?(+) dendritic cells. Oncoimmunology. 2012;1:581-592 pubmed
Ouchi T, Kubo A, Yokouchi M, Adachi T, Kobayashi T, Kitashima D, et al. Langerhans cell antigen capture through tight junctions confers preemptive immunity in experimental staphylococcal scalded skin syndrome. J Exp Med. 2011;208:2607-13 pubmed publisher
Li J, Hsu H, Yang P, Wu Q, Li H, Edgington L, et al. Treatment of arthritis by macrophage depletion and immunomodulation: testing an apoptosis-mediated therapy in a humanized death receptor mouse model. Arthritis Rheum. 2012;64:1098-109 pubmed publisher
Kmieciak M, Toor A, Graham L, Bear H, Manjili M. Ex vivo expansion of tumor-reactive T cells by means of bryostatin 1/ionomycin and the common gamma chain cytokines formulation. J Vis Exp. 2011;: pubmed publisher
Mohamed W, Sethi S, Darji A, Mraheil M, Hain T, Chakraborty T. Antibody targeting the ferritin-like protein controls Listeria infection. Infect Immun. 2010;78:3306-14 pubmed publisher
product information
Antigen :
CD16/32
Apps. Abbrev. :
Flow Cytometry
Cat # :
101337
Clone :
93
Item :
Brilliant Violet 711™ anti-mouse CD16/32
Isotype :
Rat IgG2a, λ
Other Names :
Fcγ R III/II, Ly-17
Size :
50 μg
Price (USD) :
286 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV711
Immunogen :
Sorted pre-B cells
Application Notes :
Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2. The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 and blocking of Fc-mediated reactions in functional studies 2,4,23 . It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 10 6 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA