product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-mouse CD16/32
catalog :
101318
quantity :
100 μg
price :
190 USD
clonality :
monoclonal
host :
rat
conjugate :
PE/Cy7
clone name :
93
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 31
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1d
Cai Z, Kotzin J, Ramdas B, Chen S, Nelanuthala S, Palam L, et al. Inhibition of Inflammatory Signaling in Tet2 Mutant Preleukemic Cells Mitigates Stress-Induced Abnormalities and Clonal Hematopoiesis. Cell Stem Cell. 2018;23:833-849.e5 pubmed publisher
Gruber C, Patel R, Trachtman R, Lepow L, Amanat F, Krammer F, et al. Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell. 2020;183:982-995.e14 pubmed publisher
Hidalgo San Jose L, Sunshine M, Dillingham C, Chua B, Krutá M, Hong Y, et al. Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal. Cell Rep. 2020;30:69-80.e6 pubmed publisher
Yamashita M, Passegue E. TNF-α Coordinates Hematopoietic Stem Cell Survival and Myeloid Regeneration. Cell Stem Cell. 2019;: pubmed publisher
Qi X, Li F, Wu Y, Cheng C, Han P, Wang J, et al. Optimization of 4-1BB antibody for cancer immunotherapy by balancing agonistic strength with FcγR affinity. Nat Commun. 2019;10:2141 pubmed publisher
Chen Z, Amro E, Becker F, Holzer M, Rasa S, Njeru S, et al. Cohesin-mediated NF-κB signaling limits hematopoietic stem cell self-renewal in aging and inflammation. J Exp Med. 2019;216:152-175 pubmed publisher
Vasamsetti S, Florentin J, Coppin E, Stiekema L, Zheng K, Nisar M, et al. Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation. Immunity. 2018;49:93-106.e7 pubmed publisher
Jee D, Yang J, Park S, Farmer D, Wen J, Chou T, et al. Dual Strategies for Argonaute2-Mediated Biogenesis of Erythroid miRNAs Underlie Conserved Requirements for Slicing in Mammals. Mol Cell. 2018;69:265-278.e6 pubmed publisher
Duarte D, Hawkins E, Akinduro O, Ang H, De Filippo K, Kong I, et al. Inhibition of Endosteal Vascular Niche Remodeling Rescues Hematopoietic Stem Cell Loss in AML. Cell Stem Cell. 2018;22:64-77.e6 pubmed publisher
Perez Mazliah D, Nguyen M, Hosking C, McLaughlin S, Lewis M, Tumwine I, et al. Follicular Helper T Cells are Essential for the Elimination of Plasmodium Infection. EBioMedicine. 2017;24:216-230 pubmed publisher
Zysset D, Weber B, Rihs S, Brasseit J, Freigang S, Riether C, et al. TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis. Nat Commun. 2016;7:13151 pubmed publisher
Shimizu T, Kubovcakova L, Nienhold R, Zmajkovic J, Meyer S, Hao Shen H, et al. Loss of Ezh2 synergizes with JAK2-V617F in initiating myeloproliferative neoplasms and promoting myelofibrosis. J Exp Med. 2016;213:1479-96 pubmed publisher
Bouffi C, Kartashov A, Schollaert K, Chen X, Bacon W, Weirauch M, et al. Transcription Factor Repertoire of Homeostatic Eosinophilopoiesis. J Immunol. 2015;195:2683-95 pubmed publisher
Everard A, Geurts L, Caesar R, Van Hul M, Matamoros S, Duparc T, et al. Intestinal epithelial MyD88 is a sensor switching host metabolism towards obesity according to nutritional status. Nat Commun. 2014;5:5648 pubmed publisher
Kimura T, Kobayashi S, Hanihara Tatsuzawa F, Sayama A, Maruyama T, Muta T. Responses of macrophages to the danger signals released from necrotic cells. Int Immunol. 2014;26:697-704 pubmed publisher
Cekanaviciute E, Dietrich H, Axtell R, Williams A, Egusquiza R, Wai K, et al. Astrocytic TGF-? signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection. J Immunol. 2014;193:139-49 pubmed publisher
Richardson M, Fu C, Pennington L, Honeycutt J, Odegaard J, Odegaard J, et al. A new mouse model for female genital schistosomiasis. PLoS Negl Trop Dis. 2014;8:e2825 pubmed publisher
Takei S, Omoto C, Kitagawa K, Morishita N, Katayama T, Shigemura K, et al. Oral administration of genetically modified Bifidobacterium displaying HCV-NS3 multi-epitope fusion protein could induce an HCV-NS3-specific systemic immune response in mice. Vaccine. 2014;32:3066-74 pubmed publisher
Fan Y, Mao R, Yu Y, Liu S, Shi Z, Cheng J, et al. USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase. J Exp Med. 2014;211:313-28 pubmed publisher
Huang H, Pan C, Chan Y, Chen J, Wu C. Use of the antimicrobial peptide pardaxin (GE33) to protect against methicillin-resistant Staphylococcus aureus infection in mice with skin injuries. Antimicrob Agents Chemother. 2014;58:1538-45 pubmed publisher
Dutow P, Fehlhaber B, Bode J, Laudeley R, Rheinheimer C, Glage S, et al. The complement C3a receptor is critical in defense against Chlamydia psittaci in mouse lung infection and required for antibody and optimal T cell response. J Infect Dis. 2014;209:1269-78 pubmed publisher
Hegde V, Tomar S, Jackson A, Rao R, Yang X, Singh U, et al. Distinct microRNA expression profile and targeted biological pathways in functional myeloid-derived suppressor cells induced by ?9-tetrahydrocannabinol in vivo: regulation of CCAAT/enhancer-binding protein ? by microRNA-690. J Biol Chem. 2013;288:36810-26 pubmed publisher
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Huang J, Li X, Kohno K, Hatano M, Tokuhisa T, Murray P, et al. Generation of tissue-specific H-2Kd transgenic mice for the study of K(d)-restricted malaria epitope-specific CD8+ T-cell responses in vivo. J Immunol Methods. 2013;387:254-61 pubmed publisher
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product information
Antigen :
CD16/32
Apps. Abbrev. :
Flow Cytometry
Cat # :
101318
Clone :
93
Item :
PE/Cyanine7 anti-mouse CD16/32
Isotype :
Rat IgG2a, λ
Other Names :
Fcγ R III/II, Ly-17
Size :
100 μg
Price (USD) :
190 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE_CYANINE7
Immunogen :
Sorted pre-B cells
Application Notes :
Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2. The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 and blocking of Fc-mediated reactions in functional studies 2,4,23 . It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 10 6 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA