product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse CD16/32
catalog :
101314
quantity :
100 μg
price :
215 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
93
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 26
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s6
Morales Hernández A, Martinat A, Chabot A, Kang G, McKinney Freeman S. Elevated Oxidative Stress Impairs Hematopoietic Progenitor Function in C57BL/6 Substrains. Stem Cell Reports. 2018;11:334-347 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5
Stremmel C, Schuchert R, Wagner F, Thaler R, Weinberger T, Pick R, et al. Yolk sac macrophage progenitors traffic to the embryo during defined stages of development. Nat Commun. 2018;9:75 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Kurkewich J, Boucher A, Klopfenstein N, Baskar R, Kapur R, Dahl R. The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation. Exp Hematol. 2018;59:14-29 pubmed publisher
Rosser E, Piper C, Matei D, Blair P, Rendeiro A, Orford M, et al. Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells. Cell Metab. 2020;31:837-851.e10 pubmed publisher
van der Poel C, Bajic G, Macaulay C, van den Broek T, Ellson C, Bouma G, et al. Follicular Dendritic Cells Modulate Germinal Center B Cell Diversity through FcγRIIB. Cell Rep. 2019;29:2745-2755.e4 pubmed publisher
Piper C, Rosser E, Oleinika K, Nistala K, Krausgruber T, Rendeiro A, et al. Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells. Cell Rep. 2019;29:1878-1892.e7 pubmed publisher
Stivala S, Codilupi T, Brkic S, Baerenwaldt A, Ghosh N, Hao Shen H, et al. Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms. J Clin Invest. 2019;130:1596-1611 pubmed publisher
Nestorowa S, Hamey F, Pijuan Sala B, Diamanti E, Shepherd M, Laurenti E, et al. A single-cell resolution map of mouse hematopoietic stem and progenitor cell differentiation. Blood. 2016;128:e20-31 pubmed publisher
Everard A, Geurts L, Caesar R, Van Hul M, Matamoros S, Duparc T, et al. Intestinal epithelial MyD88 is a sensor switching host metabolism towards obesity according to nutritional status. Nat Commun. 2014;5:5648 pubmed publisher
Kimura T, Kobayashi S, Hanihara Tatsuzawa F, Sayama A, Maruyama T, Muta T. Responses of macrophages to the danger signals released from necrotic cells. Int Immunol. 2014;26:697-704 pubmed publisher
Coquery C, Wade N, Loo W, Kinchen J, Cox K, Jiang C, et al. Neutrophils contribute to excess serum BAFF levels and promote CD4+ T cell and B cell responses in lupus-prone mice. PLoS ONE. 2014;9:e102284 pubmed publisher
Cekanaviciute E, Dietrich H, Axtell R, Williams A, Egusquiza R, Wai K, et al. Astrocytic TGF-? signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection. J Immunol. 2014;193:139-49 pubmed publisher
Richardson M, Fu C, Pennington L, Honeycutt J, Odegaard J, Odegaard J, et al. A new mouse model for female genital schistosomiasis. PLoS Negl Trop Dis. 2014;8:e2825 pubmed publisher
Takei S, Omoto C, Kitagawa K, Morishita N, Katayama T, Shigemura K, et al. Oral administration of genetically modified Bifidobacterium displaying HCV-NS3 multi-epitope fusion protein could induce an HCV-NS3-specific systemic immune response in mice. Vaccine. 2014;32:3066-74 pubmed publisher
Fan Y, Mao R, Yu Y, Liu S, Shi Z, Cheng J, et al. USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase. J Exp Med. 2014;211:313-28 pubmed publisher
Huang H, Pan C, Chan Y, Chen J, Wu C. Use of the antimicrobial peptide pardaxin (GE33) to protect against methicillin-resistant Staphylococcus aureus infection in mice with skin injuries. Antimicrob Agents Chemother. 2014;58:1538-45 pubmed publisher
Dutow P, Fehlhaber B, Bode J, Laudeley R, Rheinheimer C, Glage S, et al. The complement C3a receptor is critical in defense against Chlamydia psittaci in mouse lung infection and required for antibody and optimal T cell response. J Infect Dis. 2014;209:1269-78 pubmed publisher
Hegde V, Tomar S, Jackson A, Rao R, Yang X, Singh U, et al. Distinct microRNA expression profile and targeted biological pathways in functional myeloid-derived suppressor cells induced by ?9-tetrahydrocannabinol in vivo: regulation of CCAAT/enhancer-binding protein ? by microRNA-690. J Biol Chem. 2013;288:36810-26 pubmed publisher
Yamazaki S, Maruyama A, Okada K, Matsumoto M, Morita A, Seya T. Dendritic cells from oral cavity induce Foxp3(+) regulatory T cells upon antigen stimulation. PLoS ONE. 2012;7:e51665 pubmed publisher
Huang J, Li X, Kohno K, Hatano M, Tokuhisa T, Murray P, et al. Generation of tissue-specific H-2Kd transgenic mice for the study of K(d)-restricted malaria epitope-specific CD8+ T-cell responses in vivo. J Immunol Methods. 2013;387:254-61 pubmed publisher
Koon H, Ho S, Cheng M, Ichikawa R, Pothoulakis C. Differentiating functional roles of gene expression from immune and non-immune cells in mouse colitis by bone marrow transplantation. J Vis Exp. 2012;:e4208 pubmed publisher
Azuma M, Ebihara T, Oshiumi H, Matsumoto M, Seya T. Cross-priming for antitumor CTL induced by soluble Ag + polyI:C depends on the TICAM-1 pathway in mouse CD11c(+)/CD8?(+) dendritic cells. Oncoimmunology. 2012;1:581-592 pubmed
Ouchi T, Kubo A, Yokouchi M, Adachi T, Kobayashi T, Kitashima D, et al. Langerhans cell antigen capture through tight junctions confers preemptive immunity in experimental staphylococcal scalded skin syndrome. J Exp Med. 2011;208:2607-13 pubmed publisher
Li J, Hsu H, Yang P, Wu Q, Li H, Edgington L, et al. Treatment of arthritis by macrophage depletion and immunomodulation: testing an apoptosis-mediated therapy in a humanized death receptor mouse model. Arthritis Rheum. 2012;64:1098-109 pubmed publisher
Kmieciak M, Toor A, Graham L, Bear H, Manjili M. Ex vivo expansion of tumor-reactive T cells by means of bryostatin 1/ionomycin and the common gamma chain cytokines formulation. J Vis Exp. 2011;: pubmed publisher
Mohamed W, Sethi S, Darji A, Mraheil M, Hain T, Chakraborty T. Antibody targeting the ferritin-like protein controls Listeria infection. Infect Immun. 2010;78:3306-14 pubmed publisher
product information
Antigen :
CD16/32
Apps. Abbrev. :
Flow Cytometry
Cat # :
101314
Clone :
93
Item :
Alexa Fluor® 647 anti-mouse CD16/32
Isotype :
Rat IgG2a, λ
Other Names :
Fcγ R III/II, Ly-17
Size :
100 μg
Price (USD) :
215 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
Sorted pre-B cells
Application Notes :
Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2. The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 and blocking of Fc-mediated reactions in functional studies 2,4,23 . It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 10 6 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA