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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-mouse CD16/32
catalog :
101305
quantity :
50 μg
price :
77 USD
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
93
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 3d
Lu J, Wang W, Li P, Wang X, Gao C, Zhang B, et al. MiR-146a regulates regulatory T cells to suppress heart transplant rejection in mice. Cell Death Discov. 2021;7:165 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5b
Anania J, Westin A, Adler J, Heyman B. A Novel Image Analysis Approach Reveals a Role for Complement Receptors 1 and 2 in Follicular Dendritic Cell Organization in Germinal Centers. Front Immunol. 2021;12:655753 pubmed publisher
Nita A, Muto Y, Katayama Y, Matsumoto A, Nishiyama M, Nakayama K. The autism-related protein CHD8 contributes to the stemness and differentiation of mouse hematopoietic stem cells. Cell Rep. 2021;34:108688 pubmed publisher
Wilkes M, Siva K, Chen J, Varetti G, Youn M, Chae H, et al. Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase. Nat Commun. 2020;11:3344 pubmed publisher
Lang J, Bohn P, Bhat H, Jastrow H, Walkenfort B, Cansiz F, et al. Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease. Nat Commun. 2020;11:1338 pubmed publisher
Yang C, Hou X, Feng Q, Li Y, Wang X, Qin L, et al. Lupus serum IgG induces microglia activation through Fc fragment dependent way and modulated by B-cell activating factor. J Transl Med. 2019;17:426 pubmed publisher
Esposito E, Ahn B, Shi J, Nakamura Y, Park J, Mandeville E, et al. Brain-to-cervical lymph node signaling after stroke. Nat Commun. 2019;10:5306 pubmed publisher
Jiao S, Subudhi S, Aparicio A, Ge Z, Guan B, Miura Y, et al. Differences in Tumor Microenvironment Dictate T Helper Lineage Polarization and Response to Immune Checkpoint Therapy. Cell. 2019;179:1177-1190.e13 pubmed publisher
Zhu Y, Padgett L, Dinh H, Marcovecchio P, Blatchley A, Wu R, et al. Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow. Cell Rep. 2018;24:2329-2341.e8 pubmed publisher
Yamamoto R, Wilkinson A, Ooehara J, Lan X, Lai C, Nakauchi Y, et al. Large-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment. Cell Stem Cell. 2018;22:600-607.e4 pubmed publisher
Macdougall C, Wood E, Loschko J, Scagliotti V, Cassidy F, Robinson M, et al. Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets. Cell Metab. 2018;27:588-601.e4 pubmed publisher
Peraki I, Palis J, Mavrothalassitis G. The Ets2 Repressor Factor (Erf) Is Required for Effective Primitive and Definitive Hematopoiesis. Mol Cell Biol. 2017;37: pubmed publisher
Qiu J, Wang L, Zhang R, Ge K, Guo H, Liu X, et al. Identification of a TNF-α inducer MIC3 originating from the microneme of non-cystogenic, virulent Toxoplasma gondii. Sci Rep. 2016;6:39407 pubmed publisher
Everard A, Geurts L, Caesar R, Van Hul M, Matamoros S, Duparc T, et al. Intestinal epithelial MyD88 is a sensor switching host metabolism towards obesity according to nutritional status. Nat Commun. 2014;5:5648 pubmed publisher
Kimura T, Kobayashi S, Hanihara Tatsuzawa F, Sayama A, Maruyama T, Muta T. Responses of macrophages to the danger signals released from necrotic cells. Int Immunol. 2014;26:697-704 pubmed publisher
Cekanaviciute E, Dietrich H, Axtell R, Williams A, Egusquiza R, Wai K, et al. Astrocytic TGF-? signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection. J Immunol. 2014;193:139-49 pubmed publisher
Richardson M, Fu C, Pennington L, Honeycutt J, Odegaard J, Odegaard J, et al. A new mouse model for female genital schistosomiasis. PLoS Negl Trop Dis. 2014;8:e2825 pubmed publisher
Takei S, Omoto C, Kitagawa K, Morishita N, Katayama T, Shigemura K, et al. Oral administration of genetically modified Bifidobacterium displaying HCV-NS3 multi-epitope fusion protein could induce an HCV-NS3-specific systemic immune response in mice. Vaccine. 2014;32:3066-74 pubmed publisher
Fan Y, Mao R, Yu Y, Liu S, Shi Z, Cheng J, et al. USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase. J Exp Med. 2014;211:313-28 pubmed publisher
Huang H, Pan C, Chan Y, Chen J, Wu C. Use of the antimicrobial peptide pardaxin (GE33) to protect against methicillin-resistant Staphylococcus aureus infection in mice with skin injuries. Antimicrob Agents Chemother. 2014;58:1538-45 pubmed publisher
Dutow P, Fehlhaber B, Bode J, Laudeley R, Rheinheimer C, Glage S, et al. The complement C3a receptor is critical in defense against Chlamydia psittaci in mouse lung infection and required for antibody and optimal T cell response. J Infect Dis. 2014;209:1269-78 pubmed publisher
Hegde V, Tomar S, Jackson A, Rao R, Yang X, Singh U, et al. Distinct microRNA expression profile and targeted biological pathways in functional myeloid-derived suppressor cells induced by ?9-tetrahydrocannabinol in vivo: regulation of CCAAT/enhancer-binding protein ? by microRNA-690. J Biol Chem. 2013;288:36810-26 pubmed publisher
Yamazaki S, Maruyama A, Okada K, Matsumoto M, Morita A, Seya T. Dendritic cells from oral cavity induce Foxp3(+) regulatory T cells upon antigen stimulation. PLoS ONE. 2012;7:e51665 pubmed publisher
Huang J, Li X, Kohno K, Hatano M, Tokuhisa T, Murray P, et al. Generation of tissue-specific H-2Kd transgenic mice for the study of K(d)-restricted malaria epitope-specific CD8+ T-cell responses in vivo. J Immunol Methods. 2013;387:254-61 pubmed publisher
Koon H, Ho S, Cheng M, Ichikawa R, Pothoulakis C. Differentiating functional roles of gene expression from immune and non-immune cells in mouse colitis by bone marrow transplantation. J Vis Exp. 2012;:e4208 pubmed publisher
Azuma M, Ebihara T, Oshiumi H, Matsumoto M, Seya T. Cross-priming for antitumor CTL induced by soluble Ag + polyI:C depends on the TICAM-1 pathway in mouse CD11c(+)/CD8?(+) dendritic cells. Oncoimmunology. 2012;1:581-592 pubmed
Ouchi T, Kubo A, Yokouchi M, Adachi T, Kobayashi T, Kitashima D, et al. Langerhans cell antigen capture through tight junctions confers preemptive immunity in experimental staphylococcal scalded skin syndrome. J Exp Med. 2011;208:2607-13 pubmed publisher
Li J, Hsu H, Yang P, Wu Q, Li H, Edgington L, et al. Treatment of arthritis by macrophage depletion and immunomodulation: testing an apoptosis-mediated therapy in a humanized death receptor mouse model. Arthritis Rheum. 2012;64:1098-109 pubmed publisher
Kmieciak M, Toor A, Graham L, Bear H, Manjili M. Ex vivo expansion of tumor-reactive T cells by means of bryostatin 1/ionomycin and the common gamma chain cytokines formulation. J Vis Exp. 2011;: pubmed publisher
Lebert Ghali C, Fournier M, Dickson G, Thompson A, Sauvageau G, Bijl J. HoxA cluster is haploinsufficient for activity of hematopoietic stem and progenitor cells. Exp Hematol. 2010;38:1074-1086.e1-5 pubmed publisher
Mohamed W, Sethi S, Darji A, Mraheil M, Hain T, Chakraborty T. Antibody targeting the ferritin-like protein controls Listeria infection. Infect Immun. 2010;78:3306-14 pubmed publisher
Spillmann F, Beck Engeser G, Wabl M. Differentiation and Ig-allele switch in cell line WEHI-231. J Immunol. 2007;179:6395-402 pubmed
product information
Antigen :
CD16/32
Apps. Abbrev. :
Flow Cytometry
Cat # :
101305
Clone :
93
Item :
FITC anti-mouse CD16/32
Isotype :
Rat IgG2a, λ
Other Names :
Fcγ R III/II, Ly-17
Size :
50 μg
Price (USD) :
77 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
FITC
Immunogen :
Sorted pre-B cells
Application Notes :
Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2. The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 and blocking of Fc-mediated reactions in functional studies 2,4,23 . It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 10 6 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA