product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 650™ anti-mouse/human CD11b
catalog :
101259
quantity :
50 μg
price :
273 USD
clonality :
monoclonal
host :
rat
conjugate :
BV650
clone name :
M1/70
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 68
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; fig 3e
Taniguchi H, Caeser R, Chavan S, Zhan Y, Chow A, Manoj P, et al. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022;39:110814 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 5e
Ziaei A, Garcia Miralles M, Radulescu C, Sidik H, Silvin A, Bae H, et al. Ermin deficiency leads to compromised myelin, inflammatory milieu, and susceptibility to demyelinating insult. Brain Pathol. 2022;32:e13064 pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig s6g
Lin J, Liu H, Fukumoto T, Zundell J, Yan Q, Tang C, et al. Targeting the IRE1α/XBP1s pathway suppresses CARM1-expressing ovarian cancer. Nat Commun. 2021;12:5321 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a, s3b, s7c
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 3g
Chakarov S, Lim H, Tan L, Lim S, See P, Lum J, et al. Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches. Science. 2019;363: pubmed publisher
  • flow cytometry; mouse; fig s2
Wiesner D, Specht C, Lee C, Smith K, Mukaremera L, Lee S, et al. Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection. PLoS Pathog. 2015;11:e1004701 pubmed publisher
Shen X, Li Q, Li H, Wang X, Wang Q, Zheng X, et al. Antibody-Dependent Enhancement of SARS-CoV-2 Infection of Human Immune Cells: In Vitro Assessment Provides Insight in COVID-19 Pathogenesis. Viruses. 2021;13: pubmed publisher
Xu C, Sun S, Johnson T, Qi R, Zhang S, Zhang J, et al. The glutathione peroxidase Gpx4 prevents lipid peroxidation and ferroptosis to sustain Treg cell activation and suppression of antitumor immunity. Cell Rep. 2021;35:109235 pubmed publisher
Dai X, Bu X, Gao Y, Guo J, Hu J, Jiang C, et al. Energy status dictates PD-L1 protein abundance and anti-tumor immunity to enable checkpoint blockade. Mol Cell. 2021;81:2317-2331.e6 pubmed publisher
Shreeve N, Depierreux D, Hawkes D, Traherne J, Sovio U, Huhn O, et al. The CD94/NKG2A inhibitory receptor educates uterine NK cells to optimize pregnancy outcomes in humans and mice. Immunity. 2021;54:1231-1244.e4 pubmed publisher
Gern B, Adams K, Plumlee C, Stoltzfus C, Shehata L, Moguche A, et al. TGFβ restricts expansion, survival, and function of T cells within the tuberculous granuloma. Cell Host Microbe. 2021;29:594-606.e6 pubmed publisher
Chakraborty M, Chu K, Shrestha A, Revelo X, Zhang X, Gold M, et al. Mechanical Stiffness Controls Dendritic Cell Metabolism and Function. Cell Rep. 2021;34:108609 pubmed publisher
Brestoff J, Wilen C, Moley J, Li Y, Zou W, Malvin N, et al. Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in Obesity. Cell Metab. 2021;33:270-282.e8 pubmed publisher
Marín Franco J, Genoula M, Corral D, Duette G, Ferreyra M, Maio M, et al. Host-Derived Lipids from Tuberculous Pleurisy Impair Macrophage Microbicidal-Associated Metabolic Activity. Cell Rep. 2020;33:108547 pubmed publisher
Jaeger N, McDonough R, Rosen A, Hernandez Leyva A, Wilson N, Lint M, et al. Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell Rep. 2020;33:108331 pubmed publisher
Liu Z, Gu Y, Shin A, Zhang S, Ginhoux F. Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry. STAR Protoc. 2020;1:100029 pubmed publisher
Lees J, White D, Keating B, Barkl Luke M, Makker P, Goldstein D, et al. Oxaliplatin-induced haematological toxicity and splenomegaly in mice. PLoS ONE. 2020;15:e0238164 pubmed publisher
Gniadek T, Augustin L, Schottel J, Leonard A, Saltzman D, Greeno E, et al. A Phase I, Dose Escalation, Single Dose Trial of Oral Attenuated Salmonella typhimurium Containing Human IL-2 in Patients With Metastatic Gastrointestinal Cancers. J Immunother. 2020;43:217-221 pubmed publisher
Ni J, Wang X, Stojanovic A, Zhang Q, Wincher M, Bühler L, et al. Single-Cell RNA Sequencing of Tumor-Infiltrating NK Cells Reveals that Inhibition of Transcription Factor HIF-1α Unleashes NK Cell Activity. Immunity. 2020;52:1075-1087.e8 pubmed publisher
Riedel R, Addo R, Ferreira Gomes M, Heinz G, Heinrich F, Kummer J, et al. Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow. Nat Commun. 2020;11:2570 pubmed publisher
Grubman A, Vandekolk T, Schröder J, Sun G, Hatwell Humble J, Chan J, et al. A CX3CR1 Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture. Stem Cell Reports. 2020;14:1018-1032 pubmed publisher
Demircioglu F, Wang J, Candido J, Costa A, Casado P, de Luxán Delgado B, et al. Cancer associated fibroblast FAK regulates malignant cell metabolism. Nat Commun. 2020;11:1290 pubmed publisher
Benyoucef A, Marchitto L, Touzot F. CRISPR gene-engineered CYBBko THP-1 cell lines highlight the crucial role of NADPH-induced reactive oxygen species for regulating inflammasome activation. J Allergy Clin Immunol. 2020;145:1690-1693.e5 pubmed publisher
Maniati E, Berlato C, Gopinathan G, Heath O, Kotantaki P, Lakhani A, et al. Mouse Ovarian Cancer Models Recapitulate the Human Tumor Microenvironment and Patient Response to Treatment. Cell Rep. 2020;30:525-540.e7 pubmed publisher
Rosenbaum S, Knecht M, Mollaee M, Zhong Z, Erkes D, McCue P, et al. FOXD3 Regulates VISTA Expression in Melanoma. Cell Rep. 2020;30:510-524.e6 pubmed publisher
Blagih J, Zani F, Chakravarty P, Hennequart M, Pilley S, Hobor S, et al. Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses. Cell Rep. 2020;30:481-496.e6 pubmed publisher
O Boyle C, Haley M, Lemarchand E, Smith C, Allan S, Konkel J, et al. Ligature-induced periodontitis induces systemic inflammation but does not alter acute outcome after stroke in mice. Int J Stroke. 2020;15:175-187 pubmed publisher
Stifter S, Bhattacharyya N, Sawyer A, Cootes T, Stambas J, Doyle S, et al. Visualizing the Selectivity and Dynamics of Interferon Signaling In Vivo. Cell Rep. 2019;29:3539-3550.e4 pubmed publisher
Tuit S, Salvagno C, Kapellos T, Hau C, Seep L, Oestreich M, et al. Transcriptional Signature Derived from Murine Tumor-Associated Macrophages Correlates with Poor Outcome in Breast Cancer Patients. Cell Rep. 2019;29:1221-1235.e5 pubmed publisher
Benechet A, De Simone G, Di Lucia P, Cilenti F, Barbiera G, Le Bert N, et al. Dynamics and genomic landscape of CD8+ T cells undergoing hepatic priming. Nature. 2019;574:200-205 pubmed publisher
Liu Z, Gu Y, Chakarov S, Blériot C, Kwok I, Chen X, et al. Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells. Cell. 2019;178:1509-1525.e19 pubmed publisher
Hagan T, Cortese M, Rouphael N, Boudreau C, Linde C, Maddur M, et al. Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans. Cell. 2019;178:1313-1328.e13 pubmed publisher
Chen Y, Chen R, Ahmad S, Verma R, Kasturi S, Amaya L, et al. N6-Methyladenosine Modification Controls Circular RNA Immunity. Mol Cell. 2019;: pubmed publisher
Su W, Han H, Wang Y, Zhang B, Zhou B, Cheng Y, et al. The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression. Cancer Cell. 2019;36:139-155.e10 pubmed publisher
Li Y, Zou W, Brestoff J, Rohatgi N, Wu X, Atkinson J, et al. Fat-Produced Adipsin Regulates Inflammatory Arthritis. Cell Rep. 2019;27:2809-2816.e3 pubmed publisher
Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
Blecher Gonen R, Bost P, Hilligan K, David E, Salame T, Roussel E, et al. Single-Cell Analysis of Diverse Pathogen Responses Defines a Molecular Roadmap for Generating Antigen-Specific Immunity. Cell Syst. 2019;8:109-121.e6 pubmed publisher
Li H, van der Leun A, Yofe I, Lubling Y, Gelbard Solodkin D, van Akkooi A, et al. Dysfunctional CD8 T Cells Form a Proliferative, Dynamically Regulated Compartment within Human Melanoma. Cell. 2019;176:775-789.e18 pubmed publisher
Teo T, Lum F, Ghaffar K, Chan Y, Amrun S, Tan J, et al. Plasmodium co-infection protects against chikungunya virus-induced pathologies. Nat Commun. 2018;9:3905 pubmed publisher
Evrard M, Kwok I, Chong S, Teng K, Becht E, Chen J, et al. Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity. 2018;48:364-379.e8 pubmed publisher
Haertel E, Joshi N, Hiebert P, Kopf M, Werner S. Regulatory T cells are required for normal and activin-promoted wound repair in mice. Eur J Immunol. 2018;48:1001-1013 pubmed publisher
Costa A, Kieffer Y, Scholer Dahirel A, Pelon F, Bourachot B, Cardon M, et al. Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer. Cancer Cell. 2018;33:463-479.e10 pubmed publisher
Thion M, Low D, Silvin A, Chen J, Grisel P, Schulte Schrepping J, et al. Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner. Cell. 2018;172:500-516.e16 pubmed publisher
Bittner Eddy P, Fischer L, Tu A, Allman D, Costalonga M. Discriminating between Interstitial and Circulating Leukocytes in Tissues of the Murine Oral Mucosa Avoiding Nasal-Associated Lymphoid Tissue Contamination. Front Immunol. 2017;8:1398 pubmed publisher
Yu X, Buttgereit A, Lelios I, Utz S, Cansever D, Becher B, et al. The Cytokine TGF-β Promotes the Development and Homeostasis of Alveolar Macrophages. Immunity. 2017;47:903-912.e4 pubmed publisher
Jenkins R, Aref A, Lizotte P, Ivanova E, Stinson S, Zhou C, et al. Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids. Cancer Discov. 2018;8:196-215 pubmed publisher
Sierro F, Evrard M, Rizzetto S, Melino M, Mitchell A, Flórido M, et al. A Liver Capsular Network of Monocyte-Derived Macrophages Restricts Hepatic Dissemination of Intraperitoneal Bacteria by Neutrophil Recruitment. Immunity. 2017;47:374-388.e6 pubmed publisher
Gimblet C, Meisel J, Loesche M, Cole S, Horwinski J, Novais F, et al. Cutaneous Leishmaniasis Induces a Transmissible Dysbiotic Skin Microbiota that Promotes Skin Inflammation. Cell Host Microbe. 2017;22:13-24.e4 pubmed publisher
Lechner A, Driver I, Lee J, Conroy C, Nagle A, Locksley R, et al. Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy. Cell Stem Cell. 2017;21:120-134.e7 pubmed publisher
Gundra U, Girgis N, Gonzalez M, San Tang M, Van Der Zande H, Lin J, et al. Vitamin A mediates conversion of monocyte-derived macrophages into tissue-resident macrophages during alternative activation. Nat Immunol. 2017;18:642-653 pubmed publisher
Zhu Y, Lyapichev K, Lee D, Motti D, Ferraro N, Zhang Y, et al. Macrophage Transcriptional Profile Identifies Lipid Catabolic Pathways That Can Be Therapeutically Targeted after Spinal Cord Injury. J Neurosci. 2017;37:2362-2376 pubmed publisher
Yu Y, O Koren E, Hotten D, Kan M, Kopin D, Nelson E, et al. A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues. PLoS ONE. 2016;11:e0150606 pubmed publisher
Colliou N, Sahay B, Zadeh M, Owen J, Mohamadzadeh M. Impact of Gastrointestinal Bacillus anthracis Infection on Hepatic B Cells. Toxins (Basel). 2015;7:3805-17 pubmed publisher
Ferreira R, Pinto Ribeiro I, Wen X, Marcos Pinto R, Dinis Ribeiro M, Carneiro F, et al. Helicobacter pylori cagA Promoter Region Sequences Influence CagA Expression and Interleukin 8 Secretion. J Infect Dis. 2016;213:669-73 pubmed publisher
Doni A, Musso T, Morone D, Bastone A, Zambelli V, Sironi M, et al. An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode. J Exp Med. 2015;212:905-25 pubmed publisher
Price P, Bánki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, et al. Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara. J Immunol. 2015;194:1164-8 pubmed publisher
Ashok A, Rai N, Tripathi S, Bandyopadhyay S. Exposure to As-, Cd-, and Pb-mixture induces Aβ, amyloidogenic APP processing and cognitive impairments via oxidative stress-dependent neuroinflammation in young rats. Toxicol Sci. 2015;143:64-80 pubmed publisher
Moreno M, Bannerman P, Ma J, Guo F, Miers L, Soulika A, et al. Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE. J Neurosci. 2014;34:8175-85 pubmed publisher
Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, et al. Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med. 2014;211:1243-56 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Baumgartner C, Ferrante A, Nagaoka M, Gorski J, Malherbe L. Peptide-MHC class II complex stability governs CD4 T cell clonal selection. J Immunol. 2010;184:573-81 pubmed publisher
Norian L, Rodriguez P, O Mara L, Zabaleta J, Ochoa A, Cella M, et al. Tumor-infiltrating regulatory dendritic cells inhibit CD8+ T cell function via L-arginine metabolism. Cancer Res. 2009;69:3086-94 pubmed publisher
Kim D, Miwa T, Kimura Y, Schwendener R, van Lookeren Campagne M, Song W. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood. 2008;112:1109-19 pubmed publisher
Elgazar Carmon V, Rudich A, Hadad N, Levy R. Neutrophils transiently infiltrate intra-abdominal fat early in the course of high-fat feeding. J Lipid Res. 2008;49:1894-903 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Napimoga M, Vieira S, Dal Secco D, Freitas A, Souto F, Mestriner F, et al. Peroxisome proliferator-activated receptor-gamma ligand, 15-deoxy-Delta12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway. J Immunol. 2008;180:609-17 pubmed
Rasmussen J, Cello J, Gil H, Forestal C, Furie M, Thanassi D, et al. Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis. Infect Immun. 2006;74:6590-8 pubmed
Tan S, Zhao J, Bi C, Chen X, Hepburn D, Wang J, et al. Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice. J Immunol. 2006;176:2872-9 pubmed
product information
Antigen :
CD11b
Apps. Abbrev. :
Flow Cytometry
Cat # :
101259
Clone :
M1/70
Item :
Brilliant Violet 650™ anti-mouse/human CD11b
Isotype :
Rat IgG2b, κ
Other Names :
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Size :
50 μg
Price (USD) :
273 USD
Reactivity :
Mouse, Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV650
Immunogen :
C57BL/10 splenocytes
Application Notes :
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1,4 , in vitro blocking 3,9,12 , depletion 2,8 , immunofluorescence microscopy 6,7,10 , immunohistochemistry of acetone-fixed frozen sections 5,11-13 , and spatial biology (IBEX) 35,36 . For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 101248).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA