product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 594 anti-mouse/human CD11b
catalog :
101254
quantity :
100 μg
price :
237 USD
clonality :
monoclonal
host :
rat
conjugate :
Alexa Fluor 594
clone name :
M1/70
reactivity :
human, mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:1000; loading ...; fig 2d
Arinrad S, Wilke J, Seelbach A, Doeren J, Hindermann M, Butt U, et al. NMDAR1 autoantibodies amplify behavioral phenotypes of genetic white matter inflammation: a mild encephalitis model with neuropsychiatric relevance. Mol Psychiatry. 2021;: pubmed publisher
  • flow cytometry; human; loading ...; fig s3b
Fahy N, Palomares Cabeza V, Lolli A, Witte Bouma J, Merino A, Ridwan Y, et al. Chondrogenically Primed Human Mesenchymal Stem Cells Persist and Undergo Early Stages of Endochondral Ossification in an Immunocompetent Xenogeneic Model. Front Immunol. 2021;12:715267 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s3a
Yang C, Lei L, Collins J, Briones M, Ma L, Sturdevant G, et al. Chlamydia evasion of neutrophil host defense results in NLRP3 dependent myeloid-mediated sterile inflammation through the purinergic P2X7 receptor. Nat Commun. 2021;12:5454 pubmed publisher
  • flow cytometry; mouse; 1:1500; loading ...; fig s2d
Rizvi Z, Dalal R, Sadhu S, Kumar Y, Kumar S, Gupta S, et al. High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity. Sci Adv. 2021;7:eabg5016 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Neumann S, Campbell K, Woodall M, Evans M, Clarkson A, Young S. Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
Gurley J, Gmyrek G, Hargis E, Bishop G, Carr D, Elliott M. The Chx10-Traf3 Knockout Mouse as a Viable Model to Study Neuronal Immune Regulation. Cells. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Cortés A, Solas M, Pejenaute A, Abellanas M, Garcia Lacarte M, Aymerich M, et al. Expression of Endothelial NOX5 Alters the Integrity of the Blood-Brain Barrier and Causes Loss of Memory in Aging Mice. Antioxidants (Basel). 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Petermann M, Orfanos Z, Sellau J, Gharaibeh M, Lotter H, Fleischer B, et al. CCR2 Deficiency Impairs Ly6Clo and Ly6Chi Monocyte Responses in Orientia tsutsugamushi Infection. Front Immunol. 2021;12:670219 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Innamarato P, Morse J, Mackay A, Asby S, Beatty M, Blauvelt J, et al. Intralesional injection of rose bengal augments the efficacy of gemcitabine chemotherapy against pancreatic tumors. BMC Cancer. 2021;21:756 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 1a
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5d
Chen J, Cao X, Li B, Zhao Z, Chen S, Lai S, et al. Warburg Effect Is a Cancer Immune Evasion Mechanism Against Macrophage Immunosurveillance. Front Immunol. 2020;11:621757 pubmed publisher
  • flow cytometry; mouse; loading ...
Yoon S, Song S, Shin J, Kang S, Kim H, You H. Protective Effects of Korean Herbal Remedy against Airway Inflammation in an Allergic Asthma by Suppressing Eosinophil Recruitment and Infiltration in Lung. Antioxidants (Basel). 2020;10: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6a
Cignarella F, Filipello F, Bollman B, Cantoni C, Locca A, Mikesell R, et al. TREM2 activation on microglia promotes myelin debris clearance and remyelination in a model of multiple sclerosis. Acta Neuropathol. 2020;140:513-534 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 3d
Mezö C, Dokalis N, Mossad O, Staszewski O, Neuber J, Yilmaz B, et al. Different effects of constitutive and induced microbiota modulation on microglia in a mouse model of Alzheimer's disease. Acta Neuropathol Commun. 2020;8:119 pubmed publisher
  • blocking or activating experiments; mouse; 20 ug/ml; loading ...; fig 3a, 4c
  • flow cytometry; mouse; 1:100; fig s1a, s2a
Azcutia V, Kelm M, Luissint A, Boerner K, Flemming S, Quirós M, et al. Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo. Mucosal Immunol. 2020;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Wang S, Li R, Ng T, Luthria G, Oudin M, Prytyskach M, et al. Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy. Sci Adv. 2020;6:eaaz8521 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1v1, 2s1
Baba T, Miyazaki D, Inata K, Uotani R, Miyake H, Sasaki S, et al. Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degeneration. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Ray S, Chee L, Matson D, Palermo N, Bresnick E, Hewitt K. Sterile α-motif domain requirement for cellular signaling and survival. J Biol Chem. 2020;295:7113-7125 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Chao Y, Liang C, Tao H, Du Y, Wu D, Dong Z, et al. Localized cocktail chemoimmunotherapy after in situ gelation to trigger robust systemic antitumor immune responses. Sci Adv. 2020;6:eaaz4204 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 3a
Monzon Casanova E, Matheson L, Tabbada K, Zarnack K, Smith C, Turner M. Polypyrimidine tract-binding proteins are essential for B cell development. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4, s6
Eastman A, Xu J, Bermik J, Potchen N, den Dekker A, Neal L, et al. Epigenetic stabilization of DC and DC precursor classical activation by TNFα contributes to protective T cell polarization. Sci Adv. 2019;5:eaaw9051 pubmed publisher
  • flow cytometry; mouse; 1-2 ug/ml; fig 2a
Duan S, Koziol White C, Jester W, Nycholat C, Macauley M, Panettieri R, et al. CD33 recruitment inhibits IgE-mediated anaphylaxis and desensitizes mast cells to allergen. J Clin Invest. 2019;129:1387-1401 pubmed publisher
  • flow cytometry; mouse; fig 2c
Ishizuka J, Manguso R, Cheruiyot C, Bi K, Panda A, Iracheta Vellve A, et al. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature. 2019;565:43-48 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Kong I, Rimes J, Light A, Todorovski I, Jones S, Morand E, et al. Temporal Analysis of Brd4 Displacement in the Control of B Cell Survival, Proliferation, and Differentiation. Cell Rep. 2020;33:108290 pubmed publisher
Riquelme S, Liimatta K, Wong Fok Lung T, Fields B, Ahn D, Chen D, et al. Pseudomonas aeruginosa Utilizes Host-Derived Itaconate to Redirect Its Metabolism to Promote Biofilm Formation. Cell Metab. 2020;31:1091-1106.e6 pubmed publisher
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
Han P, Hanlon D, Arshad N, Lee J, Tatsuno K, Robinson E, et al. Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. Sci Adv. 2020;6:eaaz1580 pubmed publisher
Lee C, Seo I, Chae M, Park J, Choi E, Uhm H, et al. Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation. Oxid Med Cell Longev. 2020;2020:2946820 pubmed publisher
Acker K, Wong Fok Lung T, West E, Craft J, Narechania A, Smith H, et al. Strains of Staphylococcus aureus that Colonize and Infect Skin Harbor Mutations in Metabolic Genes. iScience. 2019;19:281-290 pubmed publisher
Patel S, Dhande I, Gray E, Ali Q, Hussain T. Prevention of lipopolysaccharide-induced CD11b+ immune cell infiltration in the kidney: role of AT2 receptors. Biosci Rep. 2019;39: pubmed publisher
Bradford B, Donaldson D, Forman R, Else K, Mabbott N. Increased susceptibility to oral Trichuris muris infection in the specific absence of CXCR5+ CD11c+ cells. Parasite Immunol. 2018;40:e12566 pubmed publisher
Gaiser M, Weis C, Gaiser T, Jiang H, Buder Bakhaya K, Herpel E, et al. Merkel cell carcinoma expresses the immunoregulatory ligand CD200 and induces immunosuppressive macrophages and regulatory T cells. Oncoimmunology. 2018;7:e1426517 pubmed publisher
Ferreira R, Pinto Ribeiro I, Wen X, Marcos Pinto R, Dinis Ribeiro M, Carneiro F, et al. Helicobacter pylori cagA Promoter Region Sequences Influence CagA Expression and Interleukin 8 Secretion. J Infect Dis. 2016;213:669-73 pubmed publisher
Doni A, Musso T, Morone D, Bastone A, Zambelli V, Sironi M, et al. An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode. J Exp Med. 2015;212:905-25 pubmed publisher
Price P, Bánki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, et al. Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara. J Immunol. 2015;194:1164-8 pubmed publisher
Ashok A, Rai N, Tripathi S, Bandyopadhyay S. Exposure to As-, Cd-, and Pb-mixture induces Aβ, amyloidogenic APP processing and cognitive impairments via oxidative stress-dependent neuroinflammation in young rats. Toxicol Sci. 2015;143:64-80 pubmed publisher
Chiba S, Ikushima H, Ueki H, Yanai H, Kimura Y, Hangai S, et al. Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses. elife. 2014;3:e04177 pubmed publisher
Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, et al. Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med. 2014;211:1243-56 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Baumgartner C, Ferrante A, Nagaoka M, Gorski J, Malherbe L. Peptide-MHC class II complex stability governs CD4 T cell clonal selection. J Immunol. 2010;184:573-81 pubmed publisher
Norian L, Rodriguez P, O Mara L, Zabaleta J, Ochoa A, Cella M, et al. Tumor-infiltrating regulatory dendritic cells inhibit CD8+ T cell function via L-arginine metabolism. Cancer Res. 2009;69:3086-94 pubmed publisher
Kim D, Miwa T, Kimura Y, Schwendener R, van Lookeren Campagne M, Song W. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood. 2008;112:1109-19 pubmed publisher
Elgazar Carmon V, Rudich A, Hadad N, Levy R. Neutrophils transiently infiltrate intra-abdominal fat early in the course of high-fat feeding. J Lipid Res. 2008;49:1894-903 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Napimoga M, Vieira S, Dal Secco D, Freitas A, Souto F, Mestriner F, et al. Peroxisome proliferator-activated receptor-gamma ligand, 15-deoxy-Delta12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway. J Immunol. 2008;180:609-17 pubmed
Rasmussen J, Cello J, Gil H, Forestal C, Furie M, Thanassi D, et al. Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis. Infect Immun. 2006;74:6590-8 pubmed
Tan S, Zhao J, Bi C, Chen X, Hepburn D, Wang J, et al. Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice. J Immunol. 2006;176:2872-9 pubmed
product information
Antigen :
CD11b
Apps. Abbrev. :
IHC-F, Flow Cytometry
Cat # :
101254
Clone :
M1/70
Item :
Alexa Fluor® 594 anti-mouse/human CD11b
Isotype :
Rat IgG2b, κ
Other Names :
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Size :
100 μg
Price (USD) :
237 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A594
Immunogen :
C57BL/10 splenocytes
Application Notes :
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1,4 , in vitro blocking 3,9,12 , depletion 2,8 , immunofluorescence microscopy 6,7,10 , immunohistochemistry of acetone-fixed frozen sections 5,11-13 , and spatial biology (IBEX) 35,36 . For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 101248).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA