product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-mouse/human CD11b
catalog :
101251
quantity :
50 μg
price :
242 USD
clonality :
monoclonal
host :
rat
conjugate :
BV421
clone name :
M1/70
reactivity :
human, mouse, rat
application :
flow cytometry
more info or order :
citations: 83
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig s3a
Yang C, Lei L, Collins J, Briones M, Ma L, Sturdevant G, et al. Chlamydia evasion of neutrophil host defense results in NLRP3 dependent myeloid-mediated sterile inflammation through the purinergic P2X7 receptor. Nat Commun. 2021;12:5454 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Sugita J, Fujiu K, Nakayama Y, Matsubara T, Matsuda J, Oshima T, et al. Cardiac macrophages prevent sudden death during heart stress. Nat Commun. 2021;12:1910 pubmed publisher
  • flow cytometry; rat; loading ...; fig s4b
Helms T, Mullins R, Thomas Ahner J, Kulp S, Campbell M, Lucas F, et al. Inhibition of androgen/AR signaling inhibits diethylnitrosamine (DEN) induced tumour initiation and remodels liver immune cell networks. Sci Rep. 2021;11:3646 pubmed publisher
  • flow cytometry; mouse; 1:130; loading ...; fig s6, s9, 4
Aslan K, Turco V, Blobner J, Sonner J, Liuzzi A, Núñez N, et al. Heterogeneity of response to immune checkpoint blockade in hypermutated experimental gliomas. Nat Commun. 2020;11:931 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s19b
Wang G, Xu J, Zhao J, Yin W, Liu D, Chen W, et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nat Commun. 2020;11:220 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig e1a, e1c
Vagnozzi R, Maillet M, Sargent M, Khalil H, Johansen A, Schwanekamp J, et al. An acute immune response underlies the benefit of cardiac stem cell therapy. Nature. 2020;577:405-409 pubmed publisher
  • flow cytometry; mouse; fig 1
Doni A, Musso T, Morone D, Bastone A, Zambelli V, Sironi M, et al. An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode. J Exp Med. 2015;212:905-25 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Quattrocelli M, Wintzinger M, Miz K, Panta M, Prabakaran A, Barish G, et al. Intermittent prednisone treatment in mice promotes exercise tolerance in obesity through adiponectin. J Exp Med. 2022;219: pubmed publisher
Alikhanyan K, Chen Y, Somogyi K, Kraut S, Sotillo R. Mad2 Induced Aneuploidy Contributes to Eml4-Alk Driven Lung Cancer by Generating an Immunosuppressive Environment. Cancers (Basel). 2021;13: pubmed publisher
Bae S, Park P, Lee Y, Mun S, Giannopoulou E, Fujii T, et al. MYC-mediated early glycolysis negatively regulates proinflammatory responses by controlling IRF4 in inflammatory macrophages. Cell Rep. 2021;35:109264 pubmed publisher
Chetty A, Darby M, Vornewald P, Martín Alonso M, Filz A, Ritter M, et al. Il4ra-independent vaginal eosinophil accumulation following helminth infection exacerbates epithelial ulcerative pathology of HSV-2 infection. Cell Host Microbe. 2021;29:579-593.e5 pubmed publisher
Singh S, Thompson J, Yilmaz B, Li H, Weis S, Sobral D, et al. Loss of α-gal during primate evolution enhanced antibody-effector function and resistance to bacterial sepsis. Cell Host Microbe. 2021;29:347-361.e12 pubmed publisher
Perner C, Flayer C, Zhu X, Aderhold P, Dewan Z, Voisin T, et al. Substance P Release by Sensory Neurons Triggers Dendritic Cell Migration and Initiates the Type-2 Immune Response to Allergens. Immunity. 2020;53:1063-1077.e7 pubmed publisher
Xu G, van Bruggen R, Gualtieri C, Moradin N, Fois A, Vallerand D, et al. Bisphosphoglycerate Mutase Deficiency Protects against Cerebral Malaria and Severe Malaria-Induced Anemia. Cell Rep. 2020;32:108170 pubmed publisher
Acharya N, Madi A, Zhang H, Klapholz M, Escobar G, Dulberg S, et al. Endogenous Glucocorticoid Signaling Regulates CD8+ T Cell Differentiation and Development of Dysfunction in the Tumor Microenvironment. Immunity. 2020;53:658-671.e6 pubmed publisher
Pribyl M, Hubackova S, Moudra A, Vancurova M, Polackova H, Stopka T, et al. Aberrantly elevated suprabasin in the bone marrow as a candidate biomarker of advanced disease state in myelodysplastic syndromes. Mol Oncol. 2020;14:2403-2419 pubmed publisher
Nakayama Y, Fujiu K, Yuki R, Oishi Y, Morioka M, Isagawa T, et al. A long noncoding RNA regulates inflammation resolution by mouse macrophages through fatty acid oxidation activation. Proc Natl Acad Sci U S A. 2020;117:14365-14375 pubmed publisher
Klemm F, Maas R, Bowman R, Kornete M, Soukup K, Nassiri S, et al. Interrogation of the Microenvironmental Landscape in Brain Tumors Reveals Disease-Specific Alterations of Immune Cells. Cell. 2020;181:1643-1660.e17 pubmed publisher
Tummers B, Mari L, Guy C, Heckmann B, Rodriguez D, Rühl S, et al. Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis. Immunity. 2020;52:994-1006.e8 pubmed publisher
Ilinykh P, Huang K, Santos R, Gilchuk P, Gunn B, Karim M, et al. Non-neutralizing Antibodies from a Marburg Infection Survivor Mediate Protection by Fc-Effector Functions and by Enhancing Efficacy of Other Antibodies. Cell Host Microbe. 2020;27:976-991.e11 pubmed publisher
Mohamed E, Sierra R, Trillo Tinoco J, Cao Y, Innamarato P, Payne K, et al. The Unfolded Protein Response Mediator PERK Governs Myeloid Cell-Driven Immunosuppression in Tumors through Inhibition of STING Signaling. Immunity. 2020;52:668-682.e7 pubmed publisher
Chen M, Yang A, Yousef H, Lee D, Chen W, Schaum N, et al. Brain Endothelial Cells Are Exquisite Sensors of Age-Related Circulatory Cues. Cell Rep. 2020;30:4418-4432.e4 pubmed publisher
Miller C, Barrett B, Chen J, Morrison J, Radomile C, Santiago M, et al. Systemic Expression of a Viral RdRP Protects against Retrovirus Infection and Disease. J Virol. 2020;94: pubmed publisher
Kodama L, Guzman E, Etchegaray J, Li Y, Sayed F, Zhou L, et al. Microglial microRNAs mediate sex-specific responses to tau pathology. Nat Neurosci. 2020;23:167-171 pubmed publisher
Krivtsov A, Evans K, Gadrey J, Eschle B, Hatton C, Uckelmann H, et al. A Menin-MLL Inhibitor Induces Specific Chromatin Changes and Eradicates Disease in Models of MLL-Rearranged Leukemia. Cancer Cell. 2019;36:660-673.e11 pubmed publisher
Qi F, Liu M, Li F, Lv Q, Wang G, Gong S, et al. Interleukin-37 Ameliorates Influenza Pneumonia by Attenuating Macrophage Cytokine Production in a MAPK-Dependent Manner. Front Microbiol. 2019;10:2482 pubmed publisher
O CONNOR T, Zhou X, Kosla J, Adili A, García Beccaria M, Kotsiliti E, et al. Age-Related Gliosis Promotes Central Nervous System Lymphoma through CCL19-Mediated Tumor Cell Retention. Cancer Cell. 2019;36:250-267.e9 pubmed publisher
Di Mitri D, Mirenda M, Vasilevska J, Calcinotto A, Delaleu N, Revandkar A, et al. Re-education of Tumor-Associated Macrophages by CXCR2 Blockade Drives Senescence and Tumor Inhibition in Advanced Prostate Cancer. Cell Rep. 2019;28:2156-2168.e5 pubmed publisher
Hou X, Chen G, Bracamonte Baran W, Choi H, Diny N, Sung J, et al. The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis. Cell Rep. 2019;28:172-189.e7 pubmed publisher
Ponzetta A, Carriero R, Carnevale S, Barbagallo M, Molgora M, Perucchini C, et al. Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors. Cell. 2019;178:346-360.e24 pubmed publisher
Rashidi M, Simpson D, Hempel A, Frank D, Petrie E, Vince A, et al. The Pyroptotic Cell Death Effector Gasdermin D Is Activated by Gout-Associated Uric Acid Crystals but Is Dispensable for Cell Death and IL-1β Release. J Immunol. 2019;: pubmed publisher
Bryson B, Rosebrock T, Tafesse F, Itoh C, Nibasumba A, Babunovic G, et al. Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis. Nat Commun. 2019;10:2329 pubmed publisher
Yousef H, Czupalla C, Lee D, Chen M, Burke A, Zera K, et al. Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nat Med. 2019;25:988-1000 pubmed publisher
Yousef H, Czupalla C, Lee D, Butcher E, Wyss Coray T. Papain-based Single Cell Isolation of Primary Murine Brain Endothelial Cells Using Flow Cytometry. Bio Protoc. 2018;8: pubmed publisher
Knox T, Sahakian E, Banik D, Hadley M, Palmer E, Noonepalle S, et al. Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells. Sci Rep. 2019;9:6136 pubmed publisher
Hirai T, Zenke Y, Yang Y, Bartholin L, Beura L, Masopust D, et al. Keratinocyte-Mediated Activation of the Cytokine TGF-β Maintains Skin Recirculating Memory CD8+ T Cells. Immunity. 2019;50:1249-1261.e5 pubmed publisher
Liu J, Zhang X, Chen K, Cheng Y, Liu S, Xia M, et al. CCR7 Chemokine Receptor-Inducible lnc-Dpf3 Restrains Dendritic Cell Migration by Inhibiting HIF-1α-Mediated Glycolysis. Immunity. 2019;50:600-615.e15 pubmed publisher
Li Q, Cheng Z, Zhou L, Darmanis S, Neff N, Okamoto J, et al. Developmental Heterogeneity of Microglia and Brain Myeloid Cells Revealed by Deep Single-Cell RNA Sequencing. Neuron. 2019;101:207-223.e10 pubmed publisher
Buzzelli J, O Connor L, Scurr M, Chung Nien Chin S, Catubig A, Ng G, et al. Overexpression of IL-11 promotes premalignant gastric epithelial hyperplasia in isolation from germline gp130-JAK-STAT driver mutations. Am J Physiol Gastrointest Liver Physiol. 2019;316:G251-G262 pubmed publisher
Grzelak A, Polakova I, Smahelova J, Vackova J, Pekarčíková L, Tachezy R, et al. Experimental Combined Immunotherapy of Tumours with Major Histocompatibility Complex Class I Downregulation. Int J Mol Sci. 2018;19: pubmed publisher
Li Z, Liu S, Xu J, Zhang X, Han D, Liu J, et al. Adult Connective Tissue-Resident Mast Cells Originate from Late Erythro-Myeloid Progenitors. Immunity. 2018;49:640-653.e5 pubmed publisher
Liakopoulos V, Jeron A, Shah A, Bruder D, Mertens P, Gorny X. Hemodialysis-related changes in phenotypical features of monocytes. Sci Rep. 2018;8:13964 pubmed publisher
Sabbagh M, Heng J, Luo C, Castanon R, Nery J, Rattner A, et al. Transcriptional and epigenomic landscapes of CNS and non-CNS vascular endothelial cells. elife. 2018;7: pubmed publisher
Sokol C, Camire R, Jones M, Luster A. The Chemokine Receptor CCR8 Promotes the Migration of Dendritic Cells into the Lymph Node Parenchyma to Initiate the Allergic Immune Response. Immunity. 2018;49:449-463.e6 pubmed publisher
Xu D, Jin T, Zhu H, Chen H, Ofengeim D, Zou C, et al. TBK1 Suppresses RIPK1-Driven Apoptosis and Inflammation during Development and in Aging. Cell. 2018;174:1477-1491.e19 pubmed publisher
Cong J, Wang X, Zheng X, Wang D, Fu B, Sun R, et al. Dysfunction of Natural Killer Cells by FBP1-Induced Inhibition of Glycolysis during Lung Cancer Progression. Cell Metab. 2018;28:243-255.e5 pubmed publisher
Zukauskas A, Mrsny R, Cortés Barrantes P, Turner J, Leong J, McCormick B. Transporters MRP1 and MRP2 Regulate Opposing Inflammatory Signals To Control Transepithelial Neutrophil Migration during Streptococcus pneumoniae Lung Infection. mSphere. 2018;3: pubmed publisher
Geng X, Wang X, Luo M, Xing M, Wu Y, Li W, et al. Induction of neutrophil apoptosis by a Bcl-2 inhibitor reduces particulate matter-induced lung inflammation. Aging (Albany NY). 2018;10:1415-1423 pubmed publisher
Ma C, Han M, Heinrich B, Fu Q, Zhang Q, Sandhu M, et al. Gut microbiome-mediated bile acid metabolism regulates liver cancer via NKT cells. Science. 2018;360: pubmed publisher
Skucha A, Ebner J, Schmöllerl J, Roth M, Eder T, César Razquin A, et al. MLL-fusion-driven leukemia requires SETD2 to safeguard genomic integrity. Nat Commun. 2018;9:1983 pubmed publisher
Laban H, Weigert A, Zink J, Elgheznawy A, Schürmann C, Günther L, et al. VASP regulates leukocyte infiltration, polarization, and vascular repair after ischemia. J Cell Biol. 2018;217:1503-1519 pubmed publisher
Cho C, Smallwood P, Nathans J. Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation. Neuron. 2017;95:1056-1073.e5 pubmed publisher
Kakizaki M, Watanabe R. IL-10 expression in pyramidal neurons after neuropathogenic coronaviral infection. Neuropathology. 2017;37:398-406 pubmed publisher
Yin Z, Raj D, Saiepour N, Van Dam D, Brouwer N, Holtman I, et al. Immune hyperreactivity of A? plaque-associated microglia in Alzheimer's disease. Neurobiol Aging. 2017;55:115-122 pubmed publisher
Harvey R, Howard V, Lemus M, Jois T, Andrews Z, Sleeman M. The Ghrelin/GOAT System Regulates Obesity-Induced Inflammation in Male Mice. Endocrinology. 2017;158:2179-2189 pubmed publisher
Currier M, Sprague L, Rizvi T, Nartker B, Chen C, Wang P, et al. Aurora A kinase inhibition enhances oncolytic herpes virotherapy through cytotoxic synergy and innate cellular immune modulation. Oncotarget. 2017;8:17412-17427 pubmed publisher
Moderzynski K, Papp S, Rauch J, Heine L, Kuehl S, Richardt U, et al. CD4+ T Cells Are as Protective as CD8+ T Cells against Rickettsia typhi Infection by Activating Macrophage Bactericidal Activity. PLoS Negl Trop Dis. 2016;10:e0005089 pubmed publisher
Reinert L, Lopušná K, Winther H, Sun C, Thomsen M, Nandakumar R, et al. Sensing of HSV-1 by the cGAS-STING pathway in microglia orchestrates antiviral defence in the CNS. Nat Commun. 2016;7:13348 pubmed publisher
Chai Y, Lee E, Gubbe J, Brekke J. 3D Cell Culture in a Self-Assembled Nanofiber Environment. PLoS ONE. 2016;11:e0162853 pubmed publisher
Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
Bennett M, Bennett F, Liddelow S, Ajami B, Zamanian J, Fernhoff N, et al. New tools for studying microglia in the mouse and human CNS. Proc Natl Acad Sci U S A. 2016;113:E1738-46 pubmed publisher
Däbritz J, Judd L, Chalinor H, Menheniott T, Giraud A. Altered gp130 signalling ameliorates experimental colitis via myeloid cell-specific STAT3 activation and myeloid-derived suppressor cells. Sci Rep. 2016;6:20584 pubmed publisher
Kim I, Mlsna L, Yoon S, Le B, Yu S, Xu D, et al. A postnatal peak in microglial development in the mouse hippocampus is correlated with heightened sensitivity to seizure triggers. Brain Behav. 2015;5:e00403 pubmed publisher
Ioannidis L, Nie C, Ly A, Ryg Cornejo V, Chiu C, Hansen D. Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient Control of Blood-Stage Malaria Infection and Promotes Severe Disease. J Immunol. 2016;196:1227-38 pubmed publisher
Richardson E, Shukla S, Nagy N, Boom W, Beck R, Zhou L, et al. ERK Signaling Is Essential for Macrophage Development. PLoS ONE. 2015;10:e0140064 pubmed publisher
Ferreira R, Pinto Ribeiro I, Wen X, Marcos Pinto R, Dinis Ribeiro M, Carneiro F, et al. Helicobacter pylori cagA Promoter Region Sequences Influence CagA Expression and Interleukin 8 Secretion. J Infect Dis. 2016;213:669-73 pubmed publisher
Mairhofer D, Ortner D, Tripp C, Schaffenrath S, Fleming V, Heger L, et al. Impaired gp100-Specific CD8(+) T-Cell Responses in the Presence of Myeloid-Derived Suppressor Cells in a Spontaneous Mouse Melanoma Model. J Invest Dermatol. 2015;135:2785-2793 pubmed publisher
Motozono C, Pearson J, De Leenheer E, Rizkallah P, Beck K, Trimby A, et al. Distortion of the Major Histocompatibility Complex Class I Binding Groove to Accommodate an Insulin-derived 10-Mer Peptide. J Biol Chem. 2015;290:18924-33 pubmed publisher
Price P, Bánki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, et al. Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara. J Immunol. 2015;194:1164-8 pubmed publisher
Ashok A, Rai N, Tripathi S, Bandyopadhyay S. Exposure to As-, Cd-, and Pb-mixture induces Aβ, amyloidogenic APP processing and cognitive impairments via oxidative stress-dependent neuroinflammation in young rats. Toxicol Sci. 2015;143:64-80 pubmed publisher
Chiba S, Ikushima H, Ueki H, Yanai H, Kimura Y, Hangai S, et al. Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses. elife. 2014;3:e04177 pubmed publisher
Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, et al. Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med. 2014;211:1243-56 pubmed publisher
Liu X, Zhang B, McBride J, Zhou K, Lee K, Zhou Y, et al. Antiangiogenic and antineuroinflammatory effects of kallistatin through interactions with the canonical Wnt pathway. Diabetes. 2013;62:4228-38 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Baumgartner C, Ferrante A, Nagaoka M, Gorski J, Malherbe L. Peptide-MHC class II complex stability governs CD4 T cell clonal selection. J Immunol. 2010;184:573-81 pubmed publisher
Norian L, Rodriguez P, O Mara L, Zabaleta J, Ochoa A, Cella M, et al. Tumor-infiltrating regulatory dendritic cells inhibit CD8+ T cell function via L-arginine metabolism. Cancer Res. 2009;69:3086-94 pubmed publisher
Kim D, Miwa T, Kimura Y, Schwendener R, van Lookeren Campagne M, Song W. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood. 2008;112:1109-19 pubmed publisher
Elgazar Carmon V, Rudich A, Hadad N, Levy R. Neutrophils transiently infiltrate intra-abdominal fat early in the course of high-fat feeding. J Lipid Res. 2008;49:1894-903 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Napimoga M, Vieira S, Dal Secco D, Freitas A, Souto F, Mestriner F, et al. Peroxisome proliferator-activated receptor-gamma ligand, 15-deoxy-Delta12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway. J Immunol. 2008;180:609-17 pubmed
Rasmussen J, Cello J, Gil H, Forestal C, Furie M, Thanassi D, et al. Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis. Infect Immun. 2006;74:6590-8 pubmed
Tan S, Zhao J, Bi C, Chen X, Hepburn D, Wang J, et al. Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice. J Immunol. 2006;176:2872-9 pubmed
product information
Antigen :
CD11b
Apps. Abbrev. :
FC
Cat # :
101251
Clone :
M1/70
Item :
Brilliant Violet 421™ anti-mouse/human CD11b
Isotype :
Rat IgG2b, κ
Other Names :
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Size :
50 μg
Price (USD) :
242 USD
Reactivity :
Mouse, Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV421
Immunogen :
C57BL/10 splenocytes
Application Notes :
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^1,4, in vitro blocking^3,9,12, depletion^2,8, immunofluorescence microscopy^6,7,10, immunohistochemistry of acetone-fixed frozen sections^5,11-13, and spatial biology (IBEX)^35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA