product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 700 anti-mouse/human CD11b
catalog :
101222
quantity :
100 μg
price :
195 USD
clonality :
monoclonal
host :
rat
conjugate :
AF700
clone name :
M1/70
reactivity :
human, mouse, chimpanzee
application :
flow cytometry
more info or order :
citations: 58
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s7c
Baryawno N, Przybylski D, Kowalczyk M, Kfoury Y, Severe N, Gustafsson K, et al. A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. Cell. 2019;177:1915-1932.e16 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 4a
Al Mamun A, Yu H, Mirza M, Romana S, McCullough L, Liu F. Myeloid cell IRF4 signaling protects neonatal brains from hypoxic ischemic encephalopathy. Neurochem Int. 2019;127:148-157 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Zhao Y, Harrison D, Song Y, Ji J, Huang J, Hui E. Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells. Cell Rep. 2018;24:379-390.e6 pubmed publisher
  • flow cytometry; mouse; 1:100; fig s2d
Mylonas K, Turner N, Bageghni S, Kenyon C, White C, McGregor K, et al. 11β-HSD1 suppresses cardiac fibroblast CXCL2, CXCL5 and neutrophil recruitment to the heart post MI. J Endocrinol. 2017;233:315-327 pubmed publisher
  • flow cytometry; mouse; fig 4
Pizzolla A, Oh D, Luong S, Prickett S, Henstridge D, Febbraio M, et al. High Fat Diet Inhibits Dendritic Cell and T Cell Response to Allergens but Does Not Impair Inhalational Respiratory Tolerance. PLoS ONE. 2016;11:e0160407 pubmed publisher
  • flow cytometry; mouse; fig 3
Kim P, Nakano H, Das P, Chen M, Rowe R, Chou S, et al. Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence. J Exp Med. 2015;212:633-48 pubmed publisher
  • flow cytometry; mouse; fig 1
Maruyama A, Shime H, Takeda Y, Azuma M, Matsumoto M, Seya T. Pam2 lipopeptides systemically increase myeloid-derived suppressor cells through TLR2 signaling. Biochem Biophys Res Commun. 2015;457:445-50 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Hu Q, Peng J, Jiang L, Li W, Su Q, Zhang J, et al. Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma. Cell Death Dis. 2020;11:925 pubmed publisher
Kim S, Saeidi S, Zhong X, Gwak S, Muna I, Park S, et al. Breast cancer cell debris diminishes therapeutic efficacy through heme oxygenase-1-mediated inactivation of M1-like tumor-associated macrophages. Neoplasia. 2020;22:606-616 pubmed publisher
Mirando A, Patil A, Rafie C, Christmas B, Pandey N, Stearns V, et al. Regulation of the tumor immune microenvironment and vascular normalization in TNBC murine models by a novel peptide. Oncoimmunology. 2020;9:1760685 pubmed publisher
Jackson Jones L, Smith P, Portman J, Magalhaes M, Mylonas K, Vermeren M, et al. Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants. Immunity. 2020;52:700-715.e6 pubmed publisher
Yamada K, Heim C, Xi X, Attri K, Wang D, Zhang W, et al. Monocyte metabolic reprogramming promotes pro-inflammatory activity and Staphylococcus aureus biofilm clearance. PLoS Pathog. 2020;16:e1008354 pubmed publisher
Domingues A, Kulkarni R, Giotopoulos G, Gupta S, Vinnenberg L, Arede L, et al. Loss of Kat2a enhances transcriptional noise and depletes acute myeloid leukemia stem-like cells. elife. 2020;9: pubmed publisher
Chen C, Yu W, Tober J, Gao P, He B, Lee K, et al. Spatial Genome Re-organization between Fetal and Adult Hematopoietic Stem Cells. Cell Rep. 2019;29:4200-4211.e7 pubmed publisher
Boehm D, Wolf M, Hall J, Wong T, Sen Kilic E, Basinger H, et al. Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from Bordetella pertussis. NPJ Vaccines. 2019;4:40 pubmed publisher
Roco J, Mesin L, Binder S, Nefzger C, Gonzalez Figueroa P, Canete P, et al. Class-Switch Recombination Occurs Infrequently in Germinal Centers. Immunity. 2019;51:337-350.e7 pubmed publisher
Yousef H, Czupalla C, Lee D, Chen M, Burke A, Zera K, et al. Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nat Med. 2019;25:988-1000 pubmed publisher
Lubkin A, Lee W, Alonzo F, Wang C, Aligo J, Keller M, et al. Staphylococcus aureus Leukocidins Target Endothelial DARC to Cause Lethality in Mice. Cell Host Microbe. 2019;25:463-470.e9 pubmed publisher
Matundan H, Ghiasi H. Herpes Simplex Virus 1 ICP22 Suppresses CD80 Expression by Murine Dendritic Cells. J Virol. 2019;93: pubmed publisher
Soberanes S, Misharin A, Jairaman A, Morales Nebreda L, McQuattie Pimentel A, Cho T, et al. Metformin Targets Mitochondrial Electron Transport to Reduce Air-Pollution-Induced Thrombosis. Cell Metab. 2019;29:335-347.e5 pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Genoud V, Marinari E, Nikolaev S, Castle J, Bukur V, Dietrich P, et al. Responsiveness to anti-PD-1 and anti-CTLA-4 immune checkpoint blockade in SB28 and GL261 mouse glioma models. Oncoimmunology. 2018;7:e1501137 pubmed publisher
Moreno A, Porchia B, Pagni R, Souza P, Pegoraro R, Rodrigues K, et al. The Combined Use of Melatonin and an Indoleamine 2,3-Dioxygenase-1 Inhibitor Enhances Vaccine-Induced Protective Cellular Immunity to HPV16-Associated Tumors. Front Immunol. 2018;9:1914 pubmed publisher
Fransén Pettersson N, Deronic A, Nilsson J, Hannibal T, Hansen L, Schmidt Christensen A, et al. The immunomodulatory quinoline-3-carboxamide paquinimod reverses established fibrosis in a novel mouse model for liver fibrosis. PLoS ONE. 2018;13:e0203228 pubmed publisher
Boehm D, Hall J, Wong T, DiVenere A, Sen Kilic E, Bevere J, et al. Evaluation of Adenylate Cyclase Toxoid Antigen in Acellular Pertussis Vaccines by Using a Bordetella pertussis Challenge Model in Mice. Infect Immun. 2018;86: pubmed publisher
Al Mamun A, Yu H, Romana S, Liu F. Inflammatory Responses are Sex Specific in Chronic Hypoxic-Ischemic Encephalopathy. Cell Transplant. 2018;27:1328-1339 pubmed publisher
Clemente Casares X, Hosseinzadeh S, Barbu I, Dick S, Macklin J, Wang Y, et al. A CD103+ Conventional Dendritic Cell Surveillance System Prevents Development of Overt Heart Failure during Subclinical Viral Myocarditis. Immunity. 2017;47:974-989.e8 pubmed publisher
Gundra U, Girgis N, Gonzalez M, San Tang M, Van Der Zande H, Lin J, et al. Vitamin A mediates conversion of monocyte-derived macrophages into tissue-resident macrophages during alternative activation. Nat Immunol. 2017;18:642-653 pubmed publisher
Li B, Haridas B, Jackson A, Cortado H, Mayne N, Kohnken R, et al. Inflammation drives renal scarring in experimental pyelonephritis. Am J Physiol Renal Physiol. 2017;312:F43-F53 pubmed publisher
Sadtler K, Allen B, Estrellas K, Housseau F, Pardoll D, Elisseeff J. The Scaffold Immune Microenvironment: Biomaterial-Mediated Immune Polarization in Traumatic and Nontraumatic Applications<sup/>. Tissue Eng Part A. 2017;23:1044-1053 pubmed publisher
Rossnagl S, Altrock E, Sens C, Kraft S, Rau K, Milsom M, et al. EDA-Fibronectin Originating from Osteoblasts Inhibits the Immune Response against Cancer. PLoS Biol. 2016;14:e1002562 pubmed publisher
Sykes D, Kfoury Y, Mercier F, Wawer M, Law J, Haynes M, et al. Inhibition of Dihydroorotate Dehydrogenase Overcomes Differentiation Blockade in Acute Myeloid Leukemia. Cell. 2016;167:171-186.e15 pubmed publisher
Spanier J, Frederick D, Taylor J, Heffernan J, Kotov D, Martinov T, et al. Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment. Nat Commun. 2016;7:11804 pubmed publisher
Jablonski K, Amici S, Webb L, Ruiz Rosado J, Popovich P, Partida Sanchez S, et al. Novel Markers to Delineate Murine M1 and M2 Macrophages. PLoS ONE. 2015;10:e0145342 pubmed publisher
Prigge J, Hoyt T, Dobrinen E, Capecchi M, Schmidt E, Meissner N. Type I IFNs Act upon Hematopoietic Progenitors To Protect and Maintain Hematopoiesis during Pneumocystis Lung Infection in Mice. J Immunol. 2015;195:5347-57 pubmed publisher
Ferreira R, Pinto Ribeiro I, Wen X, Marcos Pinto R, Dinis Ribeiro M, Carneiro F, et al. Helicobacter pylori cagA Promoter Region Sequences Influence CagA Expression and Interleukin 8 Secretion. J Infect Dis. 2016;213:669-73 pubmed publisher
Fournier M, Lebert Ghali C, Bijl J. HOXA4 provides stronger engraftment potential to short-term repopulating cells than HOXB4. Stem Cells Dev. 2015;24:2413-22 pubmed publisher
Doni A, Musso T, Morone D, Bastone A, Zambelli V, Sironi M, et al. An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode. J Exp Med. 2015;212:905-25 pubmed publisher
Mylonas K, Jenkins S, Castellan R, Rückerl D, McGregor K, Phythian Adams A, et al. The adult murine heart has a sparse, phagocytically active macrophage population that expands through monocyte recruitment and adopts an 'M2' phenotype in response to Th2 immunologic challenge. Immunobiology. 2015;220:924-33 pubmed publisher
Price P, Bánki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, et al. Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara. J Immunol. 2015;194:1164-8 pubmed publisher
Hoyt T, Dobrinen E, Kochetkova I, Meissner N. B cells modulate systemic responses to Pneumocystis murina lung infection and protect on-demand hematopoiesis via T cell-independent innate mechanisms when type I interferon signaling is absent. Infect Immun. 2015;83:743-58 pubmed publisher
Ashok A, Rai N, Tripathi S, Bandyopadhyay S. Exposure to As-, Cd-, and Pb-mixture induces Aβ, amyloidogenic APP processing and cognitive impairments via oxidative stress-dependent neuroinflammation in young rats. Toxicol Sci. 2015;143:64-80 pubmed publisher
Chiba S, Ikushima H, Ueki H, Yanai H, Kimura Y, Hangai S, et al. Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses. elife. 2014;3:e04177 pubmed publisher
Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, et al. Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med. 2014;211:1243-56 pubmed publisher
Metushi I, Cai P, Vega L, Grant D, Uetrecht J. Paradoxical attenuation of autoimmune hepatitis by oral isoniazid in wild-type and N-acetyltransferase-deficient mice. Drug Metab Dispos. 2014;42:963-73 pubmed publisher
Getts D, Terry R, Getts M, Deffrasnes C, Müller M, van Vreden C, et al. Therapeutic inflammatory monocyte modulation using immune-modifying microparticles. Sci Transl Med. 2014;6:219ra7 pubmed publisher
Quinn K, Yamamoto A, Costa A, Darrah P, Lindsay R, Hegde S, et al. Coadministration of polyinosinic:polycytidylic acid and immunostimulatory complexes modifies antigen processing in dendritic cell subsets and enhances HIV gag-specific T cell immunity. J Immunol. 2013;191:5085-96 pubmed publisher
Searles S, Gauss K, Wilkison M, Hoyt T, Dobrinen E, Meissner N. Modulation of inflammasome-mediated pulmonary immune activation by type I IFNs protects bone marrow homeostasis during systemic responses to Pneumocystis lung infection. J Immunol. 2013;191:3884-95 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Baumgartner C, Ferrante A, Nagaoka M, Gorski J, Malherbe L. Peptide-MHC class II complex stability governs CD4 T cell clonal selection. J Immunol. 2010;184:573-81 pubmed publisher
Norian L, Rodriguez P, O Mara L, Zabaleta J, Ochoa A, Cella M, et al. Tumor-infiltrating regulatory dendritic cells inhibit CD8+ T cell function via L-arginine metabolism. Cancer Res. 2009;69:3086-94 pubmed publisher
Kim D, Miwa T, Kimura Y, Schwendener R, van Lookeren Campagne M, Song W. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood. 2008;112:1109-19 pubmed publisher
Elgazar Carmon V, Rudich A, Hadad N, Levy R. Neutrophils transiently infiltrate intra-abdominal fat early in the course of high-fat feeding. J Lipid Res. 2008;49:1894-903 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Napimoga M, Vieira S, Dal Secco D, Freitas A, Souto F, Mestriner F, et al. Peroxisome proliferator-activated receptor-gamma ligand, 15-deoxy-Delta12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway. J Immunol. 2008;180:609-17 pubmed
Rasmussen J, Cello J, Gil H, Forestal C, Furie M, Thanassi D, et al. Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis. Infect Immun. 2006;74:6590-8 pubmed
Tan S, Zhao J, Bi C, Chen X, Hepburn D, Wang J, et al. Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice. J Immunol. 2006;176:2872-9 pubmed
product information
Antigen :
CD11b
Apps. Abbrev. :
Flow Cytometry
Cat # :
101222
Clone :
M1/70
Item :
Alexa Fluor® 700 anti-mouse/human CD11b
Isotype :
Rat IgG2b, κ
Other Names :
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Size :
100 μg
Price (USD) :
195 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Cynomolgus, Rhesus, Baboon, Chimpanzee,Rabbit (Lapine)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A700
Immunogen :
C57BL/10 splenocytes
Application Notes :
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1,4 , in vitro blocking 3,9,12 , depletion 2,8 , immunofluorescence microscopy 6,7,10 , and immunohistochemistry of acetone-fixed frozen sections 5,11-13 . For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA