product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse/human CD11b
catalog :
101220
quantity :
25 μg
price :
85 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
M1/70
reactivity :
human, mouse, chimpanzee
application :
flow cytometry
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 0.1 ug/ml; loading ...; fig s1g
Johnston J, Angyal A, Bauer R, Hamby S, Suvarna S, Baidžajevas K, et al. Myeloid Tribbles 1 induces early atherosclerosis via enhanced foam cell expansion. Sci Adv. 2019;5:eaax9183 pubmed publisher
  • flow cytometry; mouse; fig 1
Hua J, Stevenson W, Dohlman T, Inomata T, Tahvildari M, Calcagno N, et al. Graft Site Microenvironment Determines Dendritic Cell Trafficking Through the CCR7-CCL19/21 Axis. Invest Ophthalmol Vis Sci. 2016;57:1457-67 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 2
Cruz F, Borg Z, Goodwin M, Coffey A, Wagner D, Rocco P, et al. CD11b+ and Sca-1+ Cells Exert the Main Beneficial Effects of Systemically Administered Bone Marrow-Derived Mononuclear Cells in a Murine Model of Mixed Th2/Th17 Allergic Airway Inflammation. Stem Cells Transl Med. 2016;5:488-99 pubmed publisher
  • flow cytometry; mouse
Friedman R, Lindsay R, Lilly J, Nguyen V, Sorensen C, Jacobelli J, et al. An evolving autoimmune microenvironment regulates the quality of effector T cell restimulation and function. Proc Natl Acad Sci U S A. 2014;111:9223-8 pubmed publisher
  • flow cytometry; mouse
Kaiser P, Regoes R, Dolowschiak T, Wotzka S, Lengefeld J, Slack E, et al. Cecum lymph node dendritic cells harbor slow-growing bacteria phenotypically tolerant to antibiotic treatment. PLoS Biol. 2014;12:e1001793 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Davidson S, Efremova M, Riedel A, Mahata B, Pramanik J, Huuhtanen J, et al. Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth. Cell Rep. 2020;31:107628 pubmed publisher
Maas S, Abels E, van de Haar L, Zhang X, Morsett L, Sil S, et al. Glioblastoma hijacks microglial gene expression to support tumor growth. J Neuroinflammation. 2020;17:120 pubmed publisher
Pham T, Brewer S, Thurston T, Massis L, Honeycutt J, Lugo K, et al. Salmonella-Driven Polarization of Granuloma Macrophages Antagonizes TNF-Mediated Pathogen Restriction during Persistent Infection. Cell Host Microbe. 2020;27:54-67.e5 pubmed publisher
Nowak W, Grendas L, Sanmarco L, Estecho I, Arena Á, Eberhardt N, et al. Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR. EBioMedicine. 2019;50:290-305 pubmed publisher
Abels E, Maas S, Nieland L, Wei Z, Cheah P, Tai E, et al. Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21. Cell Rep. 2019;28:3105-3119.e7 pubmed publisher
Miller E, Zhang P, Ching K, Pugh E, Burns M. In vivo imaging reveals transient microglia recruitment and functional recovery of photoreceptor signaling after injury. Proc Natl Acad Sci U S A. 2019;116:16603-16612 pubmed publisher
Pronin A, Pham D, An W, Dvoriantchikova G, Reshetnikova G, Qiao J, et al. Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury. Front Mol Neurosci. 2019;12:36 pubmed publisher
Chen S, Chen L, Lin D, Chen S, Tsao Y, Guo H, et al. NLRP12 Regulates Anti-viral RIG-I Activation via Interaction with TRIM25. Cell Host Microbe. 2019;25:602-616.e7 pubmed publisher
Balzano M, De Grandis M, Vu Manh T, Chasson L, Bardin F, Farina A, et al. Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell Rep. 2019;26:3257-3271.e8 pubmed publisher
Silva H, Bafica A, Rodrigues Luiz G, Chi J, Santos P, Reis B, et al. Vasculature-associated fat macrophages readily adapt to inflammatory and metabolic challenges. J Exp Med. 2019;216:786-806 pubmed publisher
Farsakoglu Y, Palomino Segura M, Latino I, Zanaga S, Chatziandreou N, Pizzagalli D, et al. Influenza Vaccination Induces NK-Cell-Mediated Type-II IFN Response that Regulates Humoral Immunity in an IL-6-Dependent Manner. Cell Rep. 2019;26:2307-2315.e5 pubmed publisher
Karlen S, Miller E, Wang X, Levine E, Zawadzki R, Burns M. Monocyte infiltration rather than microglia proliferation dominates the early immune response to rapid photoreceptor degeneration. J Neuroinflammation. 2018;15:344 pubmed publisher
Li Z, Liu S, Xu J, Zhang X, Han D, Liu J, et al. Adult Connective Tissue-Resident Mast Cells Originate from Late Erythro-Myeloid Progenitors. Immunity. 2018;49:640-653.e5 pubmed publisher
Boulais P, Mizoguchi T, Zimmerman S, Nakahara F, Vivié J, Mar J, et al. The Majority of CD45- Ter119- CD31- Bone Marrow Cell Fraction Is of Hematopoietic Origin and Contains Erythroid and Lymphoid Progenitors. Immunity. 2018;49:627-639.e6 pubmed publisher
Cunha L, Yang M, Carter R, Guy C, Harris L, Crawford J, et al. LC3-Associated Phagocytosis in Myeloid Cells Promotes Tumor Immune Tolerance. Cell. 2018;175:429-441.e16 pubmed publisher
Feng Y, Liang Y, Ren J, Dai C. Canonical Wnt Signaling Promotes Macrophage Proliferation during Kidney Fibrosis. Kidney Dis (Basel). 2018;4:95-103 pubmed publisher
Laban H, Weigert A, Zink J, Elgheznawy A, Schürmann C, Günther L, et al. VASP regulates leukocyte infiltration, polarization, and vascular repair after ischemia. J Cell Biol. 2018;217:1503-1519 pubmed publisher
Pinho S, Marchand T, Yang E, Wei Q, Nerlov C, Frenette P. Lineage-Biased Hematopoietic Stem Cells Are Regulated by Distinct Niches. Dev Cell. 2018;44:634-641.e4 pubmed publisher
Oh B, Furtmüller G, Malek V, Fryer M, Brayton C, Walczak P, et al. Split Tolerance in a Murine Model of Heterotopic En Bloc Chest Wall Transplantation. Plast Reconstr Surg Glob Open. 2017;5:e1595 pubmed publisher
Sweet R, Nickerson K, Cullen J, Wang Y, Shlomchik M. B Cell-Extrinsic Myd88 and Fcer1g Negatively Regulate Autoreactive and Normal B Cell Immune Responses. J Immunol. 2017;199:885-893 pubmed publisher
Pierce H, Zhang D, Magnon C, Lucas D, Christin J, Huggins M, et al. Cholinergic Signals from the CNS Regulate G-CSF-Mediated HSC Mobilization from Bone Marrow via a Glucocorticoid Signaling Relay. Cell Stem Cell. 2017;20:648-658.e4 pubmed publisher
Chen G, Li Z, Duarte J, Esteban A, Cheloha R, Theile C, et al. Rapid capture and labeling of cells on single domain antibodies-functionalized flow cell. Biosens Bioelectron. 2017;89:789-794 pubmed publisher
Chen W, Cao Z, Sugaya S, Lopez M, Sendra V, Laver N, et al. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3. Nat Commun. 2016;7:11302 pubmed publisher
Catarinella M, Monestiroli A, Escobar G, Fiocchi A, Tran N, Aiolfi R, et al. IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment. EMBO Mol Med. 2016;8:155-70 pubmed publisher
Wongchana W, Lawlor R, Osborne B, Palaga T. Impact of Notch1 Deletion in Macrophages on Proinflammatory Cytokine Production and the Outcome of Experimental Autoimmune Encephalomyelitis. J Immunol. 2015;195:5337-46 pubmed publisher
Richardson E, Shukla S, Nagy N, Boom W, Beck R, Zhou L, et al. ERK Signaling Is Essential for Macrophage Development. PLoS ONE. 2015;10:e0140064 pubmed publisher
Ferreira R, Pinto Ribeiro I, Wen X, Marcos Pinto R, Dinis Ribeiro M, Carneiro F, et al. Helicobacter pylori cagA Promoter Region Sequences Influence CagA Expression and Interleukin 8 Secretion. J Infect Dis. 2016;213:669-73 pubmed publisher
Doni A, Musso T, Morone D, Bastone A, Zambelli V, Sironi M, et al. An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode. J Exp Med. 2015;212:905-25 pubmed publisher
Sanders K, Fox B, Bzik D. Attenuated Toxoplasma gondii Stimulates Immunity to Pancreatic Cancer by Manipulation of Myeloid Cell Populations. Cancer Immunol Res. 2015;3:891-901 pubmed publisher
Richardson E, Shukla S, Sweet D, Wearsch P, Tsichlis P, Boom W, et al. Toll-like receptor 2-dependent extracellular signal-regulated kinase signaling in Mycobacterium tuberculosis-infected macrophages drives anti-inflammatory responses and inhibits Th1 polarization of responding T cells. Infect Immun. 2015;83:2242-54 pubmed publisher
Price P, Bánki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, et al. Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara. J Immunol. 2015;194:1164-8 pubmed publisher
Ashok A, Rai N, Tripathi S, Bandyopadhyay S. Exposure to As-, Cd-, and Pb-mixture induces Aβ, amyloidogenic APP processing and cognitive impairments via oxidative stress-dependent neuroinflammation in young rats. Toxicol Sci. 2015;143:64-80 pubmed publisher
Chiba S, Ikushima H, Ueki H, Yanai H, Kimura Y, Hangai S, et al. Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses. elife. 2014;3:e04177 pubmed publisher
Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, et al. Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med. 2014;211:1243-56 pubmed publisher
Jackson A, Hegde V, Nagarkatti P, Nagarkatti M. Characterization of endocannabinoid-mediated induction of myeloid-derived suppressor cells involving mast cells and MCP-1. J Leukoc Biol. 2014;95:609-19 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Baumgartner C, Ferrante A, Nagaoka M, Gorski J, Malherbe L. Peptide-MHC class II complex stability governs CD4 T cell clonal selection. J Immunol. 2010;184:573-81 pubmed publisher
Norian L, Rodriguez P, O Mara L, Zabaleta J, Ochoa A, Cella M, et al. Tumor-infiltrating regulatory dendritic cells inhibit CD8+ T cell function via L-arginine metabolism. Cancer Res. 2009;69:3086-94 pubmed publisher
Kim D, Miwa T, Kimura Y, Schwendener R, van Lookeren Campagne M, Song W. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood. 2008;112:1109-19 pubmed publisher
Elgazar Carmon V, Rudich A, Hadad N, Levy R. Neutrophils transiently infiltrate intra-abdominal fat early in the course of high-fat feeding. J Lipid Res. 2008;49:1894-903 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Napimoga M, Vieira S, Dal Secco D, Freitas A, Souto F, Mestriner F, et al. Peroxisome proliferator-activated receptor-gamma ligand, 15-deoxy-Delta12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway. J Immunol. 2008;180:609-17 pubmed
Rasmussen J, Cello J, Gil H, Forestal C, Furie M, Thanassi D, et al. Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis. Infect Immun. 2006;74:6590-8 pubmed
Tan S, Zhao J, Bi C, Chen X, Hepburn D, Wang J, et al. Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice. J Immunol. 2006;176:2872-9 pubmed
product information
Antigen :
CD11b
Apps. Abbrev. :
Flow Cytometry
Cat # :
101220
Clone :
M1/70
Item :
Alexa Fluor® 647 anti-mouse/human CD11b
Isotype :
Rat IgG2b, κ
Other Names :
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Size :
25 μg
Price (USD) :
85 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Cynomolgus, Rhesus, Baboon, Chimpanzee,Rabbit (Lapine)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
C57BL/10 splenocytes
Application Notes :
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1,4 , in vitro blocking 3,9,12 , depletion 2,8 , immunofluorescence microscopy 6,7,10 , and immunohistochemistry of acetone-fixed frozen sections 5,11-13 . For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA