product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse/human CD11b
catalog :
101211
quantity :
25 μg
price :
33 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
M1/70
reactivity :
hamsters, human, mouse
application :
flow cytometry
more info or order :
citations: 227
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1a
Neumann S, Campbell K, Woodall M, Evans M, Clarkson A, Young S. Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; 1:2000; loading ...; fig 1a
Lebratti T, Lim Y, Cofie A, Andhey P, Jiang X, Scott J, et al. A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s2-1a
Li J, Zhang L, Zheng Y, Shao R, Liang Q, Yu W, et al. BAD inactivation exacerbates rheumatoid arthritis pathology by promoting survival of sublining macrophages. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Mildner A, Schönheit J, Giladi A, David E, Lara Astiaso D, Lorenzo Vivas E, et al. Genomic Characterization of Murine Monocytes Reveals C/EBP? Transcription Factor Dependence of Ly6C- Cells. Immunity. 2017;46:849-862.e7 pubmed publisher
  • flow cytometry; mouse; loading ...
He W, Wang C, Mu R, Liang P, Huang Z, Zhang J, et al. MiR-21 is required for anti-tumor immune response in mice: an implication for its bi-directional roles. Oncogene. 2017;36:4212-4223 pubmed publisher
  • flow cytometry; mouse; 1:125; loading ...; fig 2b
Chew W, Tabebordbar M, Cheng J, Mali P, Wu E, Ng A, et al. A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016;13:868-74 pubmed publisher
  • flow cytometry; mouse; fig 1
Pelly V, Kannan Y, Coomes S, Entwistle L, Rückerl D, Seddon B, et al. IL-4-producing ILC2s are required for the differentiation of TH2 cells following Heligmosomoides polygyrus infection. Mucosal Immunol. 2016;9:1407-1417 pubmed publisher
  • flow cytometry; human; fig 1
Sewald X, Ladinsky M, Uchil P, Beloor J, Pi R, Herrmann C, et al. Retroviruses use CD169-mediated trans-infection of permissive lymphocytes to establish infection. Science. 2015;350:563-567 pubmed publisher
  • flow cytometry; mouse; 1:250; fig s2
Castiglioni A, Corna G, Rigamonti E, Basso V, Vezzoli M, Monno A, et al. FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration. PLoS ONE. 2015;10:e0128094 pubmed publisher
  • flow cytometry; hamsters; fig 7
Watanabe S, Chan K, Wang J, Rivino L, Lok S, Vasudevan S. Dengue Virus Infection with Highly Neutralizing Levels of Cross-Reactive Antibodies Causes Acute Lethal Small Intestinal Pathology without a High Level of Viremia in Mice. J Virol. 2015;89:5847-61 pubmed publisher
  • flow cytometry; mouse
Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, et al. TGFβ signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE. 2015;10:e0117908 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Li J, Shu X, Xu J, Su S, Chan U, Mo L, et al. S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy. Nat Commun. 2022;13:1481 pubmed publisher
Xiao P, Li M, Zhou M, Zhao X, Wang C, Qiu J, et al. TTP protects against acute liver failure by regulating CCL2 and CCL5 through m6A RNA methylation. JCI Insight. 2021;6: pubmed publisher
van Geffen C, Deißler A, Beer Hammer S, Nurnberg B, Handgretinger R, Renz H, et al. Myeloid-Derived Suppressor Cells Dampen Airway Inflammation Through Prostaglandin E2 Receptor 4. Front Immunol. 2021;12:695933 pubmed publisher
Tang C, Castro Andrade C, O Meara M, Yoon S, Sato T, Brooks D, et al. Dual targeting of salt inducible kinases and CSF1R uncouples bone formation and bone resorption. elife. 2021;10: pubmed publisher
Zee B, Poels K, Yao C, Kawabata K, Wu G, Duy C, et al. Combined epigenetic and metabolic treatments overcome differentiation blockade in acute myeloid leukemia. iScience. 2021;24:102651 pubmed publisher
Cilenti F, Barbiera G, Caronni N, Iodice D, Montaldo E, Barresi S, et al. A PGE2-MEF2A axis enables context-dependent control of inflammatory gene expression. Immunity. 2021;54:1665-1682.e14 pubmed publisher
Li J, Qin X, Shi J, Wang X, Li T, Xu M, et al. A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis. elife. 2021;10: pubmed publisher
Hara T, Chanoch Myers R, Mathewson N, Myskiw C, Atta L, Bussema L, et al. Interactions between cancer cells and immune cells drive transitions to mesenchymal-like states in glioblastoma. Cancer Cell. 2021;39:779-792.e11 pubmed publisher
Shi L, Sun Z, Su W, Xu F, Xie D, Zhang Q, et al. Treg cell-derived osteopontin promotes microglia-mediated white matter repair after ischemic stroke. Immunity. 2021;54:1527-1542.e8 pubmed publisher
Riedel S, Lu C, Xie H, Nestler K, Vermunt M, Lenard A, et al. Intrinsically disordered Meningioma-1 stabilizes the BAF complex to cause AML. Mol Cell. 2021;81:2332-2348.e9 pubmed publisher
Szafran B, Borazjani A, Seay C, Carr R, Lehner R, Kaplan B, et al. Effects of Chlorpyrifos on Serine Hydrolase Activities, Lipid Mediators, and Immune Responses in Lungs of Neonatal and Adult Mice. Chem Res Toxicol. 2021;34:1556-1571 pubmed publisher
Kumari P, Russo A, Wright S, Muthupalani S, Rathinam V. Hierarchical cell-type-specific functions of caspase-11 in LPS shock and antibacterial host defense. Cell Rep. 2021;35:109012 pubmed publisher
Shen L, Hu P, Zhang Y, Ji Z, Shan X, Ni L, et al. Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation. Cell Metab. 2021;33:971-987.e6 pubmed publisher
Maruoka M, Zhang P, Mori H, Imanishi E, Packwood D, Harada H, et al. Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Mol Cell. 2021;81:1397-1410.e9 pubmed publisher
Chen R, Okeyo Owuor T, Patel R, Casey E, Cluster A, Yang W, et al. Kmt2c mutations enhance HSC self-renewal capacity and convey a selective advantage after chemotherapy. Cell Rep. 2021;34:108751 pubmed publisher
Han C, Song J, Hu J, Fu H, Feng Y, Mu R, et al. Smectite promotes probiotic biofilm formation in the gut for cancer immunotherapy. Cell Rep. 2021;34:108706 pubmed publisher
Omori S, Tsugita M, Hoshikawa Y, Morita M, Ito F, Yamaguchi S, et al. Tim4 recognizes carbon nanotubes and mediates phagocytosis leading to granuloma formation. Cell Rep. 2021;34:108734 pubmed publisher
Xu W, Snell L, Guo M, Boukhaled G, Macleod B, Li M, et al. Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection. Immunity. 2021;54:526-541.e7 pubmed publisher
Almada A, Horwitz N, Price F, Gonzalez A, Ko M, Bölükbaşı Ö, et al. FOS licenses early events in stem cell activation driving skeletal muscle regeneration. Cell Rep. 2021;34:108656 pubmed publisher
Yousif A, Ronsard L, Shah P, Omatsu T, Sangesland M, Bracamonte Moreno T, et al. The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells. Immunity. 2021;54:235-246.e5 pubmed publisher
Lee J, Villarreal O, Chen X, Zandee S, Young Y, Torok C, et al. QUAKING Regulates Microexon Alternative Splicing of the Rho GTPase Pathway and Controls Microglia Homeostasis. Cell Rep. 2020;33:108560 pubmed publisher
Li X, Zhang M, Huang X, Liang W, Li G, Lu X, et al. Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners. Nat Commun. 2020;11:6364 pubmed publisher
Honarpisheh P, Lee J, Banerjee A, Blasco Conesa M, Honarpisheh P, d Aigle J, et al. Potential caveats of putative microglia-specific markers for assessment of age-related cerebrovascular neuroinflammation. J Neuroinflammation. 2020;17:366 pubmed publisher
Messerer D, Vidoni L, Erber M, Stratmann A, Bauer J, Braun C, et al. Animal-Free Human Whole Blood Sepsis Model to Study Changes in Innate Immunity. Front Immunol. 2020;11:571992 pubmed publisher
Simoni L, Presumey J, van der Poel C, Castrillón C, Chang S, Utz P, et al. Complement C4A Regulates Autoreactive B Cells in Murine Lupus. Cell Rep. 2020;33:108330 pubmed publisher
Wan Y, Feng B, You Y, Yu J, Xu C, Dai H, et al. Microglial Displacement of GABAergic Synapses Is a Protective Event during Complex Febrile Seizures. Cell Rep. 2020;33:108346 pubmed publisher
Nosho S, Tosa I, Ono M, Hara E, Ishibashi K, Mikai A, et al. Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments. Int J Mol Sci. 2020;21: pubmed publisher
Guldner I, Wang Q, Yang L, Golomb S, Zhao Z, Lopez J, et al. CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10. Cell. 2020;183:1234-1248.e25 pubmed publisher
Zhang Y, Wilt E, Lu X. Human Isogenic Cell Line Models for Neutrophils and Myeloid-Derived Suppressor Cells. Int J Mol Sci. 2020;21: pubmed publisher
Wessel A, Kose N, Bombardi R, Roy V, Chantima W, Mongkolsapaya J, et al. Antibodies targeting epitopes on the cell-surface form of NS1 protect against Zika virus infection during pregnancy. Nat Commun. 2020;11:5278 pubmed publisher
Xiang W, Shi R, Zhang D, Kang X, Zhang L, Yuan J, et al. Dietary fats suppress the peritoneal seeding of colorectal cancer cells through the TLR4/Cxcl10 axis in adipose tissue macrophages. Signal Transduct Target Ther. 2020;5:239 pubmed publisher
Ma Y, Zabell T, Creasy A, Yang X, Chatterjee V, Villalba N, et al. Gut Ischemia Reperfusion Injury Induces Lung Inflammation via Mesenteric Lymph-Mediated Neutrophil Activation. Front Immunol. 2020;11:586685 pubmed publisher
Lowe P, Morel C, Ambade A, Iracheta Vellve A, Kwiatkowski E, Satishchandran A, et al. Chronic alcohol-induced neuroinflammation involves CCR2/5-dependent peripheral macrophage infiltration and microglia alterations. J Neuroinflammation. 2020;17:296 pubmed publisher
Yu L, Miao J, Li W, Chen N, Chen Q. Intranasal IL-4 Administration Alleviates Functional Deficits of Periventricular Leukomalacia in Neonatal Mice. Front Neurol. 2020;11:930 pubmed publisher
Hohsfield L, Najafi A, Ghorbanian Y, Soni N, Hingco E, Kim S, et al. Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS. J Neuroinflammation. 2020;17:279 pubmed publisher
Asadi Shahmirzadi A, Edgar D, Liao C, Hsu Y, Lucanic M, Asadi Shahmirzadi A, et al. Alpha-Ketoglutarate, an Endogenous Metabolite, Extends Lifespan and Compresses Morbidity in Aging Mice. Cell Metab. 2020;32:447-456.e6 pubmed publisher
Li Y, Kong W, Yang W, Patel R, Casey E, Okeyo Owuor T, et al. Single-Cell Analysis of Neonatal HSC Ontogeny Reveals Gradual and Uncoordinated Transcriptional Reprogramming that Begins before Birth. Cell Stem Cell. 2020;27:732-747.e7 pubmed publisher
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Yang L, Han Y, Nilsson Payant B, Gupta V, Wang P, Duan X, et al. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids. Cell Stem Cell. 2020;27:125-136.e7 pubmed publisher
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Ni J, Wang X, Stojanovic A, Zhang Q, Wincher M, Bühler L, et al. Single-Cell RNA Sequencing of Tumor-Infiltrating NK Cells Reveals that Inhibition of Transcription Factor HIF-1α Unleashes NK Cell Activity. Immunity. 2020;52:1075-1087.e8 pubmed publisher
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Yu X, Dai Y, Zhao Y, Qi S, Liu L, Lu L, et al. Melittin-lipid nanoparticles target to lymph nodes and elicit a systemic anti-tumor immune response. Nat Commun. 2020;11:1110 pubmed publisher
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Tripathi H, Peng H, Donahue R, Chelvarajan L, Gottipati A, Levitan B, et al. Isolation Methods for Human CD34 Subsets Using Fluorescent and Magnetic Activated Cell Sorting: an In Vivo Comparative Study. Stem Cell Rev Rep. 2020;16:413-423 pubmed publisher
Chen C, Li B, Millman S, Chen C, Li X, Morris J, et al. Vitamin B6 Addiction in Acute Myeloid Leukemia. Cancer Cell. 2020;37:71-84.e7 pubmed publisher
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Xie Y, Barbosa A, Xu M, Oberly P, Ren S, Gibbs R, et al. Hepatic Estrogen Sulfotransferase Distantly Sensitizes Mice to Hemorrhagic Shock-Induced Acute Lung Injury. Endocrinology. 2020;161: pubmed publisher
Ohgomori T, Jinno S. The expression of keratan sulfate reveals a unique subset of microglia in the mouse hippocampus after pilocarpine-induced status epileptics. J Comp Neurol. 2020;528:14-31 pubmed publisher
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Chun E, Lavoie S, Fonseca Pereira D, Bae S, Michaud M, Hoveyda H, et al. Metabolite-Sensing Receptor Ffar2 Regulates Colonic Group 3 Innate Lymphoid Cells and Gut Immunity. Immunity. 2019;51:871-884.e6 pubmed publisher
Xu H, Ding J, Porter C, Wallrapp A, Tabaka M, Ma S, et al. Transcriptional Atlas of Intestinal Immune Cells Reveals that Neuropeptide α-CGRP Modulates Group 2 Innate Lymphoid Cell Responses. Immunity. 2019;51:696-708.e9 pubmed publisher
Tsuchiya N, Zhang R, Iwama T, Ueda N, Liu T, Tatsumi M, et al. Type I Interferon Delivery by iPSC-Derived Myeloid Cells Elicits Antitumor Immunity via XCR1+ Dendritic Cells. Cell Rep. 2019;29:162-175.e9 pubmed publisher
Haloul M, Oliveira E, Kader M, Wells J, Tominello T, El Andaloussi A, et al. mTORC1-mediated polarization of M1 macrophages and their accumulation in the liver correlate with immunopathology in fatal ehrlichiosis. Sci Rep. 2019;9:14050 pubmed publisher
Gomes T, Várady C, Lourenço A, Mizurini D, Rondon A, Leal A, et al. IL-1β Blockade Attenuates Thrombosis in a Neutrophil Extracellular Trap-Dependent Breast Cancer Model. Front Immunol. 2019;10:2088 pubmed publisher
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Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
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Nowak W, Taha H, Markiewicz J, Kachamakova Trojanowska N, Stepniewski J, Kloska D, et al. Atorvastatin and Conditioned Media from Atorvastatin-Treated Human Hematopoietic Stem/Progenitor-Derived Cells Show Proangiogenic Activity In Vitro but Not In Vivo. Mediators Inflamm. 2019;2019:1868170 pubmed publisher
Zhou Z, Zhang S, Ding S, Abudupataer M, Zhang Z, Zhu X, et al. Excessive Neutrophil Extracellular Trap Formation Aggravates Acute Myocardial Infarction Injury in Apolipoprotein E Deficiency Mice via the ROS-Dependent Pathway. Oxid Med Cell Longev. 2019;2019:1209307 pubmed publisher
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Frisbee A, Saleh M, Young M, Leslie J, Simpson M, Abhyankar M, et al. IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection. Nat Commun. 2019;10:2712 pubmed publisher
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Guenther C, Faisal I, Uotila L, Asens M, Harjunpää H, Savinko T, et al. A β2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation. Front Immunol. 2019;10:1138 pubmed publisher
Chow M, Ozga A, Servis R, Frederick D, Lo J, Fisher D, et al. Intratumoral Activity of the CXCR3 Chemokine System Is Required for the Efficacy of Anti-PD-1 Therapy. Immunity. 2019;: pubmed publisher
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Paris J, Morgan M, Campos J, Spencer G, Shmakova A, Ivanova I, et al. Targeting the RNA m6A Reader YTHDF2 Selectively Compromises Cancer Stem Cells in Acute Myeloid Leukemia. Cell Stem Cell. 2019;: pubmed publisher
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Ayoub M, Shinde Jadhav S, Mansure J, Alvarez F, Connell T, Seuntjens J, et al. The immune mediated role of extracellular HMGB1 in a heterotopic model of bladder cancer radioresistance. Sci Rep. 2019;9:6348 pubmed publisher
Saleh M, Frisbee A, Leslie J, Buonomo E, Cowardin C, Ma J, et al. Colitis-Induced Th17 Cells Increase the Risk for Severe Subsequent Clostridium difficile Infection. Cell Host Microbe. 2019;25:756-765.e5 pubmed publisher
Di Genua C, Norfo R, Rodriguez Meira A, Wen W, Drissen R, Booth C, et al. Cell-intrinsic depletion of Aml1-ETO-expressing pre-leukemic hematopoietic stem cells by K-Ras activating mutation. Haematologica. 2019;: pubmed publisher
Perdomo J, Leung H, Ahmadi Z, Yan F, Chong J, Passam F, et al. Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia. Nat Commun. 2019;10:1322 pubmed publisher
Li B, SONG T, Wang F, Yin C, Li Z, Lin J, et al. Tumor-derived exosomal HMGB1 promotes esophageal squamous cell carcinoma progression through inducing PD1+ TAM expansion. Oncogenesis. 2019;8:17 pubmed publisher
Szczerba B, Castro Giner F, Vetter M, Krol I, Gkountela S, Landin J, et al. Neutrophils escort circulating tumour cells to enable cell cycle progression. Nature. 2019;566:553-557 pubmed publisher
Yu X, Chen L, Liu J, Dai B, Xu G, Shen G, et al. Immune modulation of liver sinusoidal endothelial cells by melittin nanoparticles suppresses liver metastasis. Nat Commun. 2019;10:574 pubmed publisher
Hilt Z, Pariser D, Ture S, Mohan A, Quijada P, Asante A, et al. Platelet-derived β2M regulates monocyte inflammatory responses. JCI Insight. 2019;: pubmed publisher
Domingos Pereira S, Sathiyanadan K, La Rosa S, Polák L, Chevalier M, Martel P, et al. Intravesical Ty21a Vaccine Promotes Dendritic Cells and T Cell-Mediated Tumor Regression in the MB49 Bladder Cancer Model. Cancer Immunol Res. 2019;: pubmed publisher
Sanders K, Delker D, Huecksteadt T, Beck E, Wuren T, Chen Y, et al. RAGE is a Critical Mediator of Pulmonary Oxidative Stress, Alveolar Macrophage Activation and Emphysema in Response to Cigarette Smoke. Sci Rep. 2019;9:231 pubmed publisher
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product information
Antigen :
CD11b
Apps. Abbrev. :
Flow Cytometry
Cat # :
101211
Clone :
M1/70
Item :
APC anti-mouse/human CD11b
Isotype :
Rat IgG2b, κ
Other Names :
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Size :
25 μg
Price (USD) :
33 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC
Immunogen :
C57BL/10 splenocytes
Application Notes :
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1,4 , in vitro blocking 3,9,12 , depletion 2,8 , immunofluorescence microscopy 6,7,10 , immunohistochemistry of acetone-fixed frozen sections 5,11-13 , and spatial biology (IBEX) 35,36 . For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 101248).
more info or order :
company information
BioLegend
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