product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-mouse/human CD11b
catalog :
101206
quantity :
500 μg
price :
132 USD
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
M1/70
reactivity :
human, mouse
application :
western blot, flow cytometry
more info or order :
citations: 225
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 5d
Hua X, Ge S, Zhang M, Mo F, Zhang L, Zhang J, et al. Pathogenic Roles of CXCL10 in Experimental Autoimmune Prostatitis by Modulating Macrophage Chemotaxis and Cytokine Secretion. Front Immunol. 2021;12:706027 pubmed publisher
  • flow cytometry; mouse; fig 3b
Kiepura A, Stachyra K, Wisniewska A, Kus K, Czepiel K, Suski M, et al. The Anti-Atherosclerotic Action of FFAR4 Agonist TUG-891 in ApoE-Knockout Mice Is Associated with Increased Macrophage Polarization towards M2 Phenotype. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5b, 5h
Lu C, Liu Z, Klement J, Yang D, Merting A, Poschel D, et al. WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:25; loading ...; fig 8a
Tian X, Wang Y, Lu Y, Wang W, Du J, Chen S, et al. Conditional depletion of macrophages ameliorates cholestatic liver injury and fibrosis via lncRNA-H19. Cell Death Dis. 2021;12:646 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s1j
Bielecki P, Riesenfeld S, Hütter J, Torlai Triglia E, Kowalczyk M, Ricardo Gonzalez R, et al. Skin-resident innate lymphoid cells converge on a pathogenic effector state. Nature. 2021;592:128-132 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2e
Song M, YEKU O, Rafiq S, Purdon T, Dong X, Zhu L, et al. Tumor derived UBR5 promotes ovarian cancer growth and metastasis through inducing immunosuppressive macrophages. Nat Commun. 2020;11:6298 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s1-1a
Alonso Herranz L, Sahún Español Á, Paredes A, Gonzalo P, Gkontra P, Núñez V, et al. Macrophages promote endothelial-to-mesenchymal transition via MT1-MMP/TGFβ1 after myocardial infarction. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; fig 5a
Wang L, Zheng J, Pathak J, Chen Y, Liang D, Yang L, et al. SLIT2 Overexpression in Periodontitis Intensifies Inflammation and Alveolar Bone Loss, Possibly via the Activation of MAPK Pathway. Front Cell Dev Biol. 2020;8:593 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Liu D, Bai X, Ma W, Xin D, Chu X, Yuan H, et al. Purmorphamine Attenuates Neuro-Inflammation and Synaptic Impairments After Hypoxic-Ischemic Injury in Neonatal Mice via Shh Signaling. Front Pharmacol. 2020;11:204 pubmed publisher
  • flow cytometry; mouse; 1:130; loading ...; fig 6b, c, 4e, s9b,
Aslan K, Turco V, Blobner J, Sonner J, Liuzzi A, Núñez N, et al. Heterogeneity of response to immune checkpoint blockade in hypermutated experimental gliomas. Nat Commun. 2020;11:931 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s6a
Terashima Y, Toda E, Itakura M, Otsuji M, Yoshinaga S, Okumura K, et al. Targeting FROUNT with disulfiram suppresses macrophage accumulation and its tumor-promoting properties. Nat Commun. 2020;11:609 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1g
Zhou Y, Lei J, Xie Q, Wu L, Jin S, Guo B, et al. Fibrinogen-like protein 2 controls sepsis catabasis by interacting with resolvin Dp5. Sci Adv. 2019;5:eaax0629 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5b
Nagai J, Balestrieri B, Fanning L, Kyin T, Cirka H, Lin J, et al. P2Y6 signaling in alveolar macrophages prevents leukotriene-dependent type 2 allergic lung inflammation. J Clin Invest. 2019;129:5169-5186 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2a
Geng S, Zhang Y, Lee C, Li L. Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis. Sci Adv. 2019;5:eaav2309 pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig s8
Wilgenburg B, Loh L, Chen Z, Pediongco T, Wang H, Shi M, et al. MAIT cells contribute to protection against lethal influenza infection in vivo. Nat Commun. 2018;9:4706 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 10s3a
Ku A, Shaver T, Rao A, Howard J, Rodriguez C, Miao Q, et al. TCF7L1 promotes skin tumorigenesis independently of β-catenin through induction of LCN2. elife. 2017;6: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Zhang G, Liu H, Huang J, Chen S, Pan X, Huang H, et al. TREM-1low is a novel characteristic for tumor-associated macrophages in lung cancer. Oncotarget. 2016;7:40508-40517 pubmed publisher
  • flow cytometry; mouse; fig 1
Hua J, Stevenson W, Dohlman T, Inomata T, Tahvildari M, Calcagno N, et al. Graft Site Microenvironment Determines Dendritic Cell Trafficking Through the CCR7-CCL19/21 Axis. Invest Ophthalmol Vis Sci. 2016;57:1457-67 pubmed publisher
  • flow cytometry; mouse
Tagliamonte M, Petrizzo A, Napolitano M, Luciano A, Rea D, Barbieri A, et al. A novel multi-drug metronomic chemotherapy significantly delays tumor growth in mice. J Transl Med. 2016;14:58 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 5g
Koronyo Y, Salumbides B, Sheyn J, Pelissier L, Li S, Ljubimov V, et al. Therapeutic effects of glatiramer acetate and grafted CD115⁺ monocytes in a mouse model of Alzheimer's disease. Brain. 2015;138:2399-422 pubmed publisher
  • flow cytometry; mouse; fig 4
Hegde V, Singh U, Nagarkatti P, Nagarkatti M. Critical Role of Mast Cells and Peroxisome Proliferator-Activated Receptor γ in the Induction of Myeloid-Derived Suppressor Cells by Marijuana Cannabidiol In Vivo. J Immunol. 2015;194:5211-22 pubmed publisher
  • flow cytometry; mouse; fig 1
Maruyama A, Shime H, Takeda Y, Azuma M, Matsumoto M, Seya T. Pam2 lipopeptides systemically increase myeloid-derived suppressor cells through TLR2 signaling. Biochem Biophys Res Commun. 2015;457:445-50 pubmed publisher
  • flow cytometry; mouse
Leon Rico D, Fernández García M, Aldea M, Sánchez R, Peces Barba M, Martínez Palacio J, et al. Comparison of haematopoietic stem cell engraftment through the retro-orbital venous sinus and the lateral vein: alternative routes for bone marrow transplantation in mice. Lab Anim. 2015;49:132-41 pubmed publisher
  • flow cytometry; mouse
Price P, Luckow B, Torres Domínguez L, Brandmüller C, Zorn J, Kirschning C, et al. Chemokine (C-C Motif) receptor 1 is required for efficient recruitment of neutrophils during respiratory infection with modified vaccinia virus Ankara. J Virol. 2014;88:10840-50 pubmed publisher
  • flow cytometry; mouse
Kent D, Li J, Tanna H, Fink J, Kirschner K, Pask D, et al. Self-renewal of single mouse hematopoietic stem cells is reduced by JAK2V617F without compromising progenitor cell expansion. PLoS Biol. 2013;11:e1001576 pubmed publisher
Shin S, Yoo J, Jung J, Choi J, Bae S. Inflammatory monocytes infiltrate the spiral ligament and migrate to the basilar membrane after noise exposure. Clin Exp Otorhinolaryngol. 2022;: pubmed publisher
Marks K, Flaherty S, Patterson K, STRATTON M, Martinez G, Reynolds J. Toll-like receptor 2 induces pathogenicity in Th17 cells and reveals a role for IPCEF in regulating Th17 cell migration. Cell Rep. 2021;35:109303 pubmed publisher
Okubo K, Brenner M, Cullere X, Saggu G, Patchen M, Bose N, et al. Inhibitory affinity modulation of FcγRIIA ligand binding by glycosphingolipids by inside-out signaling. Cell Rep. 2021;35:109142 pubmed publisher
Esmaili S, Langfelder P, Belgard T, Vitale D, Azardaryany M, Alipour Talesh G, et al. Core liver homeostatic co-expression networks are preserved but respond to perturbations in an organism- and disease-specific manner. Cell Syst. 2021;12:432-445.e7 pubmed publisher
Matsumoto M, Nakagawa S, Zhang L, Nakamura Y, Villaruz A, Otto M, et al. Interaction between Staphylococcus Agr virulence and neutrophils regulates pathogen expansion in the skin. Cell Host Microbe. 2021;29:930-940.e4 pubmed publisher
Tacconi C, Commerford C, Dieterich L, Schwager S, He Y, Ikenberg K, et al. CD169+ lymph node macrophages have protective functions in mouse breast cancer metastasis. Cell Rep. 2021;35:108993 pubmed publisher
Zhang S, Edwards T, Chaudhri V, Wu J, Cohen J, Hirai T, et al. Nonpeptidergic neurons suppress mast cells via glutamate to maintain skin homeostasis. Cell. 2021;184:2151-2166.e16 pubmed publisher
He Y, Fu L, Li Y, Wang W, Gong M, Zhang J, et al. Gut microbial metabolites facilitate anticancer therapy efficacy by modulating cytotoxic CD8+ T cell immunity. Cell Metab. 2021;33:988-1000.e7 pubmed publisher
Dong R, Xue Z, Fan G, Zhang N, Wang C, Li G, et al. Pin1 Promotes NLRP3 Inflammasome Activation by Phosphorylation of p38 MAPK Pathway in Septic Shock. Front Immunol. 2021;12:620238 pubmed publisher
Stahnke S, Döring H, Kusch C, de Gorter D, Dütting S, Guledani A, et al. Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. Curr Biol. 2021;31:2051-2064.e8 pubmed publisher
Yi M, Zhang J, Li A, Niu M, Yan Y, Jiao Y, et al. The construction, expression, and enhanced anti-tumor activity of YM101: a bispecific antibody simultaneously targeting TGF-β and PD-L1. J Hematol Oncol. 2021;14:27 pubmed publisher
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Liu Z, Gu Y, Shin A, Zhang S, Ginhoux F. Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry. STAR Protoc. 2020;1:100029 pubmed publisher
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Zhou J, Yao Z, Zheng Z, Yang J, Wang R, Fu S, et al. G-MDSCs-Derived Exosomal miRNA-143-3p Promotes Proliferation via Targeting of ITM2B in Lung Cancer. Onco Targets Ther. 2020;13:9701-9719 pubmed publisher
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Lin R, Zhang Y, Pradhan K, Li L. TICAM2-related pathway mediates neutrophil exhaustion. Sci Rep. 2020;10:14397 pubmed publisher
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Krotova K, Day A, Aslanidi G. An Engineered AAV6-Based Vaccine Induces High Cytolytic Anti-Tumor Activity by Directly Targeting DCs and Improves Ag Presentation. Mol Ther Oncolytics. 2019;15:166-177 pubmed publisher
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Viny A, Bowman R, Liu Y, Lavallée V, Eisman S, Xiao W, et al. Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation. Cell Stem Cell. 2019;25:682-696.e8 pubmed publisher
Wang X, Sun G, Feng T, Zhang J, Huang X, Wang T, et al. Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression. Cell Res. 2019;29:787-803 pubmed publisher
Li S, Lu X, Shao Q, Chen Z, Huang Q, Jiao Z, et al. Early Histone Deacetylase Inhibition Mitigates Ischemia/Reperfusion Brain Injury by Reducing Microglia Activation and Modulating Their Phenotype. Front Neurol. 2019;10:893 pubmed publisher
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Zong Z, Zou J, Mao R, Ma C, Li N, Wang J, et al. M1 Macrophages Induce PD-L1 Expression in Hepatocellular Carcinoma Cells Through IL-1β Signaling. Front Immunol. 2019;10:1643 pubmed publisher
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product information
Antigen :
CD11b
Apps. Abbrev. :
Flow Cytometry
Cat # :
101206
Clone :
M1/70
Item :
FITC anti-mouse/human CD11b
Isotype :
Rat IgG2b, κ
Other Names :
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Size :
500 μg
Price (USD) :
132 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
FITC
Immunogen :
C57BL/10 splenocytes
Application Notes :
Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1,4 , in vitro blocking 3,9,12 , depletion 2,8 , immunofluorescence microscopy 6,7,10 , immunohistochemistry of acetone-fixed frozen sections 5,11-13 , and spatial biology (IBEX) 35,36 . For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 101248).
more info or order :
company information
BioLegend
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