Spark Violet™ 423 anti-mouse CD8a | BioLegend 100795 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Spark Violet™ 423 anti-mouse CD8a

catalog :

100795

quantity :

25 μg

price :

129 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

53-6.7

reactivity :

mouse

application :

flow cytometry, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 45

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...	Amaral E, Foreman T, Namasivayam S, Hilligan K, Kauffman K, Barbosa Bomfim C, et al. GPX4 regulates cellular necrosis and host resistance in Mycobacterium tuberculosis infection. J Exp Med. 2022;219: pubmed publisher
flow cytometry; mouse; loading ...; fig 4b, s4b	Hou X, Shi Y, Kang X, Rousu Z, Li D, Wang M, et al. Echinococcus granulosus: The establishment of the metacestode in the liver is associated with control of the CD4+ T-cell-mediated immune response in patients with cystic echinococcosis and a mouse model. Front Cell Infect Microbiol. 2022;12:983119 pubmed publisher
flow cytometry; mouse; loading ...; fig s5a	Lee A, Pingali S, Pinilla Ibarz J, Atchison M, Koumenis C, Argon Y, et al. Loss of AID exacerbates the malignant progression of CLL. Leukemia. 2022;36:2430-2442 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Costain A, Phythian Adams A, Colombo S, Marley A, Owusu C, Cook P, et al. Dynamics of Host Immune Response Development During Schistosoma mansoni Infection. Front Immunol. 2022;13:906338 pubmed publisher
flow cytometry; mouse; fig 2f	Wu B, Song M, Dong Q, Xiang G, Li J, Ma X, et al. UBR5 promotes tumor immune evasion through enhancing IFN-γ-induced PDL1 transcription in triple negative breast cancer. Theranostics. 2022;12:5086-5102 pubmed publisher
blocking or activating experiments; mouse; 10 ug/ml; loading ...; fig 6b flow cytometry; mouse; loading ...; fig 1a, 4e, s4a	Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
flow cytometry; mouse; 1:100; fig 1f	Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
flow cytometry; mouse; loading ...; fig 3d, 4b	Koutn xed k J, Klepsch V, Pommermayr M, Thuille N, Baier G, Siegmund K. A MLR-Based Approach to Analyze Regulators of T Lymphocyte Activation In Vivo. Int J Mol Sci. 2022;23: pubmed publisher
flow cytometry; mouse; loading ...; fig s1a, 4c	Aiken T, Erbe A, Zebertavage L, Komjathy D, Feils A, Rodriguez M, et al. Mechanism of effective combination radio-immunotherapy against 9464D-GD2, an immunologically cold murine neuroblastoma. J Immunother Cancer. 2022;10: pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Pan C, Wu Q, Wang S, Mei Z, Zhang L, Gao X, et al. Combination with Toll-like receptor 4 (TLR4) agonist reverses GITR agonism mediated M2 polarization of macrophage in Hepatocellular carcinoma. Oncoimmunology. 2022;11:2073010 pubmed publisher
flow cytometry; mouse; loading ...; fig 3	Kumagai Y, Futoh Y, Miyato H, Ohzawa H, Yamaguchi H, Saito S, et al. Effect of Systemic or Intraperitoneal Administration of Anti-PD-1 Antibody for Peritoneal Metastases from Gastric Cancer. In Vivo. 2022;36:1126-1135 pubmed publisher
flow cytometry; mouse; loading ...; fig s5e, s6a	Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
flow cytometry; mouse; loading ...; fig 2b, 4c	Seung H, Wröbel J, Wadle C, B xfc hler T, Chiang D, Rettkowski J, et al. P2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction. Basic Res Cardiol. 2022;117:16 pubmed publisher
flow cytometry; mouse; loading ...; fig 2h	Quách T, Huang W, Sahu R, Diadhiou C, Raparia C, Johnson R, et al. Context-dependent induction of autoimmunity by TNF signaling deficiency. JCI Insight. 2022;7: pubmed publisher
flow cytometry; mouse; fig 1d	Kono M, Komatsuda H, Yamaki H, Kumai T, Hayashi R, Wakisaka R, et al. Immunomodulation via FGFR inhibition augments FGFR1 targeting T-cell based antitumor immunotherapy for head and neck squamous cell carcinoma. Oncoimmunology. 2022;11:2021619 pubmed publisher
flow cytometry; mouse; 1:500; loading ...; fig 2d	Arinrad S, Wilke J, Seelbach A, Doeren J, Hindermann M, Butt U, et al. NMDAR1 autoantibodies amplify behavioral phenotypes of genetic white matter inflammation: a mild encephalitis model with neuropsychiatric relevance. Mol Psychiatry. 2021;: pubmed publisher
flow cytometry; mouse	Horiuchi S, Wu H, Liu W, Schmitt N, Provot J, Liu Y, et al. Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells. Sci Adv. 2021;7:eabj1249 pubmed publisher
flow cytometry; mouse	Tanaka Y, Onozato M, Mikami T, Kohwi Shigematsu T, Fukushima T, Kondo M. Increased Indoleamine 2,3-Dioxygenase Levels at the Onset of Sjögren's Syndrome in SATB1-Conditional Knockout Mice. Int J Mol Sci. 2021;22: pubmed publisher
flow cytometry; mouse; 1:1000; loading ...; fig s2d	Rizvi Z, Dalal R, Sadhu S, Kumar Y, Kumar S, Gupta S, et al. High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity. Sci Adv. 2021;7:eabg5016 pubmed publisher
flow cytometry; mouse; loading ...; fig s13b	Wang Z, He L, Li W, Xu C, Zhang J, Wang D, et al. GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma. J Immunother Cancer. 2021;9: pubmed publisher
flow cytometry; mouse; loading ...	Neumann S, Campbell K, Woodall M, Evans M, Clarkson A, Young S. Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice. Int J Mol Sci. 2021;22: pubmed publisher
flow cytometry; mouse; loading ...	Winn N, Wolf E, Cottam M, Bhanot M, Hasty A. Myeloid-specific deletion of ferroportin impairs macrophage bioenergetics but is disconnected from systemic insulin action in adult mice. Am J Physiol Endocrinol Metab. 2021;321:E376-E391 pubmed publisher
flow cytometry; mouse	Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
flow cytometry; mouse; 1:200; loading ...	Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
flow cytometry; mouse; 1:500; loading ...; fig 6a	Wilke J, Hindermann M, Moussavi A, Butt U, Dadarwal R, Berghoff S, et al. Inducing sterile pyramidal neuronal death in mice to model distinct aspects of gray matter encephalitis. Acta Neuropathol Commun. 2021;9:121 pubmed publisher
flow cytometry; mouse; loading ...; fig 6a	Innamarato P, Morse J, Mackay A, Asby S, Beatty M, Blauvelt J, et al. Intralesional injection of rose bengal augments the efficacy of gemcitabine chemotherapy against pancreatic tumors. BMC Cancer. 2021;21:756 pubmed publisher
flow cytometry; mouse; fig 4b	Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
flow cytometry; mouse; loading ...; fig s11	Kim C, Jin J, Ye Z, Jadhav R, Gustafson C, Hu B, et al. Histone deficiency and accelerated replication stress in T cell aging. J Clin Invest. 2021;131: pubmed publisher
flow cytometry; mouse; loading ...; fig 4d	Clemen R, Freund E, Mrochen D, Miebach L, Schmidt A, Rauch B, et al. Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice. Adv Sci (Weinh). 2021;8:2003395 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig 5a	Li K, Yuan Z, Lyu J, Ahn E, Davis S, Ahmed R, et al. PD-1 suppresses TCR-CD8 cooperativity during T-cell antigen recognition. Nat Commun. 2021;12:2746 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 5b	Amoozgar Z, Kloepper J, Ren J, Tay R, Kazer S, Kiner E, et al. Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12:2582 pubmed publisher
flow cytometry; mouse; 1:1000; loading ...; fig 1b	Phong B, D Souza S, Baudier R, Wu E, Immethun V, Bauer D, et al. IgE-activated mast cells enhance TLR4-mediated antigen-specific CD4+ T cell responses. Sci Rep. 2021;11:9686 pubmed publisher
flow cytometry; mouse; loading ...; fig 1c	Petty A, Dai R, Lapalombella R, Baiocchi R, Benson D, Li Z, et al. Hedgehog-induced PD-L1 on tumor-associated macrophages is critical for suppression of tumor-infiltrating CD8+ T cell function. JCI Insight. 2021;6: pubmed publisher
flow cytometry; mouse; 1:100; loading ...	Nakajima S, Tanaka R, Yamashiro K, Chiba A, Noto D, Inaba T, et al. Mucosal-Associated Invariant T Cells Are Involved in Acute Ischemic Stroke by Regulating Neuroinflammation. J Am Heart Assoc. 2021;10:e018803 pubmed publisher
flow cytometry; mouse; loading ...; fig 2c, s4a, s4b	Can xe8 S, Van Snick J, Uyttenhove C, Pilotte L, van den Eynde B. TGFβ1 neutralization displays therapeutic efficacy through both an immunomodulatory and a non-immune tumor-intrinsic mechanism. J Immunother Cancer. 2021;9: pubmed publisher
flow cytometry; mouse; 1:100; loading ...	Page N, Lemeille S, Vincenti I, Klimek B, Mariotte A, Wagner I, et al. Persistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling. Nat Commun. 2021;12:1009 pubmed publisher
flow cytometry; mouse; 1:100; loading ...	Yao C, Lou G, Sun H, Zhu Z, Sun Y, Chen Z, et al. BACH2 enforces the transcriptional and epigenetic programs of stem-like CD8+ T cells. Nat Immunol. 2021;22:370-380 pubmed publisher
flow cytometry; mouse; 1:100; fig s16a	Li Y, Sun Y, Kulke M, Hechler T, Van der Jeught K, Dong T, et al. Targeted immunotherapy for HER2-low breast cancer with 17p loss. Sci Transl Med. 2021;13: pubmed publisher
flow cytometry; mouse; loading ...; fig s1a	Brownlie D, Doughty Shenton D, Yh Soong D, Nixon C, O Carragher N, M Carlin L, et al. Metastasis-associated macrophages constrain antitumor capability of natural killer cells in the metastatic site at least partially by membrane bound transforming growth factor β. J Immunother Cancer. 2021;9: pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig 3b	Break T, Oikonomou V, Dutzan N, Desai J, Swidergall M, Freiwald T, et al. Aberrant type 1 immunity drives susceptibility to mucosal fungal infections. Science. 2021;371: pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig 2g	Sanchez Felipe L, Vercruysse T, Sharma S, Ma J, Lemmens V, Van Looveren D, et al. A single-dose live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate. Nature. 2021;590:320-325 pubmed publisher
flow cytometry; mouse; loading ...; fig 2a, 3e, 3f	Meryk A, Grasse M, Balasco L, Kapferer W, Grubeck Loebenstein B, Pangrazzi L. Antioxidants N-Acetylcysteine and Vitamin C Improve T Cell Commitment to Memory and Long-Term Maintenance of Immunological Memory in Old Mice. Antioxidants (Basel). 2020;9: pubmed publisher
flow cytometry; mouse; loading ...; fig s18, s20	Pariser D, Hilt Z, Ture S, Blick Nitko S, Looney M, Cleary S, et al. Lung megakaryocytes are immune modulatory cells. J Clin Invest. 2021;131: pubmed publisher
flow cytometry; mouse; loading ...; fig 1d	Mahr B, Pilat N, Granofszky N, Muckenhuber M, Unger L, Weijler A, et al. Distinct roles for major and minor antigen barriers in chimerism-based tolerance under irradiation-free conditions. Am J Transplant. 2021;21:968-977 pubmed publisher
flow cytometry; mouse; 1:400; fig 1j	Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher

product information

Antigen :

CD8alpha

Apps. Abbrev. :

Cat # :

100795

Clone :

53-6.7

Item :

Spark Violet™ 423 anti-mouse CD8a

Isotype :

Rat IgG2a, κ

Other Names :

T8, Lyt2, Ly-2

Size :

25 μg

Price (USD) :

129 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

SPKVL_423

Immunogen :

Mouse thymus or spleen

Application Notes :

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes^3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a^+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation^1,3, in vivo and in vitro cell depletion^2,10,15, inhibition of CD8 T cell proliferation^3, blocking of cytotoxicity^3,4, immunohistochemical staining^5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)^29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).;

more info or order :

BioLegend product webpage