product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
TotalSeq™-A0002 anti-mouse CD8a
catalog :
100773
quantity :
10 μg
price :
358 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
53-6.7
reactivity :
mouse, rhesus macaque
application :
flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments, mass cytometry
more info or order :
citations: 96
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:500; loading ...; fig 2d
Arinrad S, Wilke J, Seelbach A, Doeren J, Hindermann M, Butt U, et al. NMDAR1 autoantibodies amplify behavioral phenotypes of genetic white matter inflammation: a mild encephalitis model with neuropsychiatric relevance. Mol Psychiatry. 2021;: pubmed publisher
  • flow cytometry; mouse
Horiuchi S, Wu H, Liu W, Schmitt N, Provot J, Liu Y, et al. Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells. Sci Adv. 2021;7:eabj1249 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2
Susukida T, Kuwahara S, Song B, Kazaoka A, Aoki S, Ito K. Regulation of the immune tolerance system determines the susceptibility to HLA-mediated abacavir-induced skin toxicity. Commun Biol. 2021;4:1137 pubmed publisher
  • flow cytometry; mouse
Tanaka Y, Onozato M, Mikami T, Kohwi Shigematsu T, Fukushima T, Kondo M. Increased Indoleamine 2,3-Dioxygenase Levels at the Onset of Sjögren's Syndrome in SATB1-Conditional Knockout Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig s2d
Rizvi Z, Dalal R, Sadhu S, Kumar Y, Kumar S, Gupta S, et al. High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity. Sci Adv. 2021;7:eabg5016 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s13b
Wang Z, He L, Li W, Xu C, Zhang J, Wang D, et al. GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; loading ...
Neumann S, Campbell K, Woodall M, Evans M, Clarkson A, Young S. Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; loading ...
Winn N, Wolf E, Cottam M, Bhanot M, Hasty A. Myeloid-specific deletion of ferroportin impairs macrophage bioenergetics but is disconnected from systemic insulin action in adult mice. Am J Physiol Endocrinol Metab. 2021;321:E376-E391 pubmed publisher
  • flow cytometry; mouse
Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig 6a
Wilke J, Hindermann M, Moussavi A, Butt U, Dadarwal R, Berghoff S, et al. Inducing sterile pyramidal neuronal death in mice to model distinct aspects of gray matter encephalitis. Acta Neuropathol Commun. 2021;9:121 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2f
Gvozdeva O, Achasova K, Litvinova N, Kozhevnikova E, Litvinova E. Female Scent Activated Expression of Arginase1 and Inducible NO-Synthetase in Lung of BALB/c Male Mice. Animals (Basel). 2021;11: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6a
Innamarato P, Morse J, Mackay A, Asby S, Beatty M, Blauvelt J, et al. Intralesional injection of rose bengal augments the efficacy of gemcitabine chemotherapy against pancreatic tumors. BMC Cancer. 2021;21:756 pubmed publisher
  • flow cytometry; mouse; fig 4b
Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s11
Kim C, Jin J, Ye Z, Jadhav R, Gustafson C, Hu B, et al. Histone deficiency and accelerated replication stress in T cell aging. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Clemen R, Freund E, Mrochen D, Miebach L, Schmidt A, Rauch B, et al. Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice. Adv Sci (Weinh). 2021;8:2003395 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 5a
Li K, Yuan Z, Lyu J, Ahn E, Davis S, Ahmed R, et al. PD-1 suppresses TCR-CD8 cooperativity during T-cell antigen recognition. Nat Commun. 2021;12:2746 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5b
Amoozgar Z, Kloepper J, Ren J, Tay R, Kazer S, Kiner E, et al. Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12:2582 pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig 1b
Phong B, D Souza S, Baudier R, Wu E, Immethun V, Bauer D, et al. IgE-activated mast cells enhance TLR4-mediated antigen-specific CD4+ T cell responses. Sci Rep. 2021;11:9686 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Petty A, Dai R, Lapalombella R, Baiocchi R, Benson D, Li Z, et al. Hedgehog-induced PD-L1 on tumor-associated macrophages is critical for suppression of tumor-infiltrating CD8+ T cell function. JCI Insight. 2021;6: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 3b
Break T, Oikonomou V, Dutzan N, Desai J, Swidergall M, Freiwald T, et al. Aberrant type 1 immunity drives susceptibility to mucosal fungal infections. Science. 2021;371: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2g
Sanchez Felipe L, Vercruysse T, Sharma S, Ma J, Lemmens V, Van Looveren D, et al. A single-dose live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate. Nature. 2021;590:320-325 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Mahr B, Pilat N, Granofszky N, Muckenhuber M, Unger L, Weijler A, et al. Distinct roles for major and minor antigen barriers in chimerism-based tolerance under irradiation-free conditions. Am J Transplant. 2021;21:968-977 pubmed publisher
  • flow cytometry; mouse; 1:400; fig 1j
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 5a
Gao L, Li B, Wang J, Shen D, Yang M, Sun R, et al. Activation of Liver X Receptor α Sensitizes Mice to T-Cell Mediated Hepatitis. Hepatol Commun. 2020;4:1664-1679 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2d
Li N, Kang Y, Wang L, Huff S, Tang R, Hui H, et al. ALKBH5 regulates anti-PD-1 therapy response by modulating lactate and suppressive immune cell accumulation in tumor microenvironment. Proc Natl Acad Sci U S A. 2020;117:20159-20170 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1s3, 4c
Manils J, Webb L, Howes A, Janzen J, Boeing S, Bowcock A, et al. CARD14E138A signalling in keratinocytes induces TNF-dependent skin and systemic inflammation. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Ning Y, Ding J, Sun X, Xie Y, Su M, Ma C, et al. HDAC9 deficiency promotes tumor progression by decreasing the CD8+ dendritic cell infiltration of the tumor microenvironment. J Immunother Cancer. 2020;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d
Bekeschus S, Clemen R, Nießner F, Sagwal S, Freund E, Schmidt A. Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion. Adv Sci (Weinh). 2020;7:1903438 pubmed publisher
  • flow cytometry; mouse; 1:800; loading ...; fig 2a
Burfeind K, Zhu X, Norgard M, Levasseur P, Huisman C, Buenafe A, et al. Circulating myeloid cells invade the central nervous system to mediate cachexia during pancreatic cancer. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3g
Zheng D, Gao F, Cheng Q, Bao P, Dong X, Fan J, et al. A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11:1985 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s3d
Wuggenig P, Kaya B, Melhem H, Ayata C, Hruz P, Sayan A, et al. Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice. Commun Biol. 2020;3:130 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Zhang S, Liang W, Luo L, Sun S, Wang F. The role of T cell trafficking in CTLA-4 blockade-induced gut immunopathology. BMC Biol. 2020;18:29 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4b
Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c, 1d, 6a
Kumar A, Chamoto K, Chowdhury P, Honjo T. Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4d
  • flow cytometry; mouse; loading ...; fig 4a, 4b
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
  • blocking or activating experiments; mouse; ; fig 4c
  • flow cytometry; mouse; 1 ug/ml; loading ...
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s16d
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7b
Hayes M, Ward S, Crawford G, Seoane R, Jackson W, Kipling D, et al. Inflammation-induced IgE promotes epithelial hyperplasia and tumour growth. elife. 2020;9: pubmed publisher
  • mass cytometry; mouse; 3 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • mass cytometry; mouse; loading ...; fig 1a, 1c, s1
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e2a, e5h
Strickley J, Messerschmidt J, Awad M, Li T, Hasegawa T, Ha D, et al. Immunity to commensal papillomaviruses protects against skin cancer. Nature. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Shikama Y, Kurosawa M, Furukawa M, Ishimaru N, Matsushita K. Involvement of adiponectin in age-related increases in tear production in mice. Aging (Albany NY). 2019;11:8329-8346 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e10
Majer O, Liu B, Kreuk L, Krogan N, Barton G. UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity. Nature. 2019;575:366-370 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Rasoulouniriana D, Santana Magal N, Gutwillig A, Farhat Younis L, Wine Y, Saperia C, et al. A distinct subset of FcγRI-expressing Th1 cells exert antibody-mediated cytotoxic activity. J Clin Invest. 2019;129:4151-4164 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Wirsching H, Zhang H, Szulzewsky F, Arora S, Grandi P, Cimino P, et al. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma. JCI Insight. 2019;4: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Papaioannou E, Yanez D, Ross S, Lau C, Solanki A, Chawda M, et al. Sonic Hedgehog signaling limits atopic dermatitis via Gli2-driven immune regulation. J Clin Invest. 2019;129:3153-3170 pubmed publisher
  • flow cytometry; mouse; loading ...; fig ex4a
Khan O, Giles J, McDonald S, Manne S, Ngiow S, Patel K, et al. TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...
Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Qiu J, Villa M, Sanin D, Buck M, O Sullivan D, Ching R, et al. Acetate Promotes T Cell Effector Function during Glucose Restriction. Cell Rep. 2019;27:2063-2074.e5 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Sharma N, Vacher J, Allison J. TLR1/2 ligand enhances antitumor efficacy of CTLA-4 blockade by increasing intratumoral Treg depletion. Proc Natl Acad Sci U S A. 2019;116:10453-10462 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Takagaki S, Yamashita R, Hashimoto N, Sugihara K, Kanari K, Tabata K, et al. Galactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow. Sci Rep. 2019;9:7133 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 3b
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 6g
Pascutti M, Geerman S, Collins N, Brasser G, Nota B, Stark R, et al. Peripheral and systemic antigens elicit an expandable pool of resident memory CD8+ T cells in the bone marrow. Eur J Immunol. 2019;49:853-872 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s2o
Lee J, Stone M, Porrett P, Thomas S, Komar C, Li J, et al. Hepatocytes direct the formation of a pro-metastatic niche in the liver. Nature. 2019;567:249-252 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s9a
Straub T, Pircher H. Enhancing immunity prevents virus-induced T-cell-mediated immunopathology in B cell-deficient mice. Eur J Immunol. 2019;49:782-789 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
McLaren J, Clement M, Marsden M, Miners K, Llewellyn Lacey S, Grant E, et al. IL-33 Augments Virus-Specific Memory T Cell Inflation and Potentiates the Efficacy of an Attenuated Cytomegalovirus-Based Vaccine. J Immunol. 2019;202:943-955 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4f
Silva D, Yu S, Ulge U, Spangler J, Jude K, Labao Almeida C, et al. De novo design of potent and selective mimics of IL-2 and IL-15. Nature. 2019;565:186-191 pubmed publisher
  • flow cytometry; mouse; fig 2a
Ishizuka J, Manguso R, Cheruiyot C, Bi K, Panda A, Iracheta Vellve A, et al. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature. 2019;565:43-48 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5d
Kaplanov I, Carmi Y, Kornetsky R, Shemesh A, Shurin G, Shurin M, et al. Blocking IL-1β reverses the immunosuppression in mouse breast cancer and synergizes with anti-PD-1 for tumor abrogation. Proc Natl Acad Sci U S A. 2019;116:1361-1369 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 2e
Chea L, Wyatt L, Gangadhara S, Moss B, Amara R. Novel Modified Vaccinia Virus Ankara Vector Expressing Anti-apoptotic Gene B13R Delays Apoptosis and Enhances Humoral Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c, 2b
Klement J, Paschall A, Redd P, Ibrahim M, Lu C, Yang D, et al. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion. J Clin Invest. 2018;128:5549-5560 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e8a
Wan X, Zinselmeyer B, Zakharov P, Vomund A, Taniguchi R, Santambrogio L, et al. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Nature. 2018;560:107-111 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3g
Jun H, Yu H, Gong J, Jiang J, Qiao X, Perkey E, et al. An immune-beige adipocyte communication via nicotinic acetylcholine receptor signaling. Nat Med. 2018;24:814-822 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Takamori A, Nambu A, Sato K, Yamaguchi S, Matsuda K, Numata T, et al. IL-31 is crucial for induction of pruritus, but not inflammation, in contact hypersensitivity. Sci Rep. 2018;8:6639 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s11
Foerster F, Boegel S, Heck R, Pickert G, R ssel N, Rosigkeit S, et al. Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells. Oncoimmunology. 2018;7:e1409929 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5c
Carrasco S, Hu S, Imai D, Kumar R, Sandusky G, Yang X, et al. Toll-like receptor 3 (TLR3) promotes the resolution of Chlamydia muridarum genital tract infection in congenic C57BL/6N mice. PLoS ONE. 2018;13:e0195165 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5a
Prado C, Gaiazzi M, Gonzalez H, Ugalde V, Figueroa A, Osorio Barrios F, et al. Dopaminergic Stimulation of Myeloid Antigen-Presenting Cells Attenuates Signal Transducer and Activator of Transcription 3-Activation Favouring the Development of Experimental Autoimmune Encephalomyelitis. Front Immunol. 2018;9:571 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2f
Lee J, Park J, Nam T, Seo S, Kim J, Lee H, et al. Differences between immunodeficient mice generated by classical gene targeting and CRISPR/Cas9-mediated gene knockout. Transgenic Res. 2018;27:241-251 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s5c
Sun H, Lagarrigue F, Gingras A, Fan Z, Ley K, Ginsberg M. Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development. J Cell Biol. 2018;217:1453-1465 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2
Lee Y, Lee J, Jang Y, Seo S, Chang J, Seong B. Non-specific Effect of Vaccines: Immediate Protection against Respiratory Syncytial Virus Infection by a Live Attenuated Influenza Vaccine. Front Microbiol. 2018;9:83 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Doorduijn E, Sluijter M, Marijt K, Querido B, van der Burg S, van Hall T. T cells specific for a TAP-independent self-peptide remain naïve in tumor-bearing mice and are fully exploitable for therapy. Oncoimmunology. 2018;7:e1382793 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Turner D, Goldklang M, Cvetkovski F, Paik D, Trischler J, Barahona J, et al. Biased Generation and In Situ Activation of Lung Tissue-Resident Memory CD4 T Cells in the Pathogenesis of Allergic Asthma. J Immunol. 2018;200:1561-1569 pubmed publisher
Guldner I, Golomb S, Wang Q, Wang E, Zhang S. Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes. STAR Protoc. 2021;2:100537 pubmed publisher
Golomb S, Guldner I, Zhao A, Wang Q, Palakurthi B, Aleksandrovic E, et al. Multi-modal Single-Cell Analysis Reveals Brain Immune Landscape Plasticity during Aging and Gut Microbiota Dysbiosis. Cell Rep. 2020;33:108438 pubmed publisher
Guldner I, Wang Q, Yang L, Golomb S, Zhao Z, Lopez J, et al. CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10. Cell. 2020;183:1234-1248.e25 pubmed publisher
Remmerie A, Martens L, Thoné T, Castoldi A, Seurinck R, Pavie B, et al. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver. Immunity. 2020;53:641-657.e14 pubmed publisher
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
Cui L, Osada K, Imaizumi A, Kataoka K, Nakano K. Feasibility of a subcutaneously administered block/homo-mixed polyplex micelle as a carrier for DNA vaccination in a mouse tumor model. J Control Release. 2015;206:220-31 pubmed publisher
del Rio M, Cote Sierra J, Rodriguez Barbosa J. Flt3L-mobilized dendritic cells bearing H2-Kbm1 apoptotic cells do not induce cross-tolerance to CD8+ T cells across a class I MHC mismatched barrier. Transpl Int. 2011;24:501-13 pubmed publisher
Apte S, Groves P, Olver S, Baz A, Doolan D, Kelso A, et al. IFN-gamma inhibits IL-4-induced type 2 cytokine expression by CD8 T cells in vivo and modulates the anti-tumor response. J Immunol. 2010;185:998-1004 pubmed publisher
Bankoti J, Burnett A, Navarro S, Miller A, Rase B, Shepherd D. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010;115:422-34 pubmed publisher
Medyouf H, Gao X, Armstrong F, Gusscott S, Liu Q, Gedman A, et al. Acute T-cell leukemias remain dependent on Notch signaling despite PTEN and INK4A/ARF loss. Blood. 2010;115:1175-84 pubmed publisher
Todd D, McHeyzer Williams L, Kowal C, Lee A, Volpe B, Diamond B, et al. XBP1 governs late events in plasma cell differentiation and is not required for antigen-specific memory B cell development. J Exp Med. 2009;206:2151-9 pubmed publisher
Riedl P, Wieland A, Lamberth K, Buus S, Lemonnier F, Reifenberg K, et al. Elimination of immunodominant epitopes from multispecific DNA-based vaccines allows induction of CD8 T cells that have a striking antiviral potential. J Immunol. 2009;183:370-80 pubmed publisher
Lee C, Chiang Y, Chang S, Chong C, Cheng B, Roffler S. Tumor-localized ligation of CD3 and CD28 with systemic regulatory T-cell depletion induces potent innate and adaptive antitumor responses. Clin Cancer Res. 2009;15:2756-66 pubmed publisher
Britschgi M, Link A, Lissandrin T, Luther S. Dynamic modulation of CCR7 expression and function on naive T lymphocytes in vivo. J Immunol. 2008;181:7681-8 pubmed
Kingeter L, Schaefer B. Loss of protein kinase C theta, Bcl10, or Malt1 selectively impairs proliferation and NF-kappa B activation in the CD4+ T cell subset. J Immunol. 2008;181:6244-54 pubmed
Geiben Lynn R, Greenland J, Frimpong Boateng K, Van Rooijen N, Hovav A, Letvin N. CD4+ T lymphocytes mediate in vivo clearance of plasmid DNA vaccine antigen expression and potentiate CD8+ T-cell immune responses. Blood. 2008;112:4585-90 pubmed publisher
Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Andrews D, Andoniou C, Fleming P, Smyth M, Degli Esposti M. The early kinetics of cytomegalovirus-specific CD8+ T-cell responses are not affected by antigen load or the absence of perforin or gamma interferon. J Virol. 2008;82:4931-7 pubmed publisher
Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
Rasmussen J, Cello J, Gil H, Forestal C, Furie M, Thanassi D, et al. Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis. Infect Immun. 2006;74:6590-8 pubmed
Kao C, Daniels M, Jameson S. Loss of CD8 and TCR binding to Class I MHC ligands following T cell activation. Int Immunol. 2005;17:1607-17 pubmed
Ko S, Ko H, Chang W, Park S, Kweon M, Kang C. alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005;175:3309-17 pubmed
product information
Antigen :
CD8a
Apps. Abbrev. :
PG
Cat # :
100773
Clone :
53-6.7
Item :
TotalSeq™-A0002 anti-mouse CD8a
Isotype :
Rat IgG2a, κ
Other Names :
T8, Lyt2, Ly-2
Size :
10 μg
Price (USD) :
358 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
TOTALSEQ_A
Immunogen :
Mouse thymus or spleen
Application Notes :
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes 3 . The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a + cells. Additional reported applications (for the relevant formats) include: immunoprecipitation 1,3 , in vivo and in vitro cell depletion 2,10,15 , inhibition of CD8 T cell proliferation 3 , blocking of cytotoxicity 3,4 , immunohistochemical staining 5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX) 29,30 . Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA