product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 510™ anti-mouse CD8a
catalog :
100752
quantity :
50 μg
price :
242 USD
clonality :
monoclonal
host :
rat
conjugate :
BV510
clone name :
53-6.7
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 74
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4d
Clemen R, Freund E, Mrochen D, Miebach L, Schmidt A, Rauch B, et al. Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice. Adv Sci (Weinh). 2021;8:2003395 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1s3, 4c
Manils J, Webb L, Howes A, Janzen J, Boeing S, Bowcock A, et al. CARD14E138A signalling in keratinocytes induces TNF-dependent skin and systemic inflammation. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d
Bekeschus S, Clemen R, Nießner F, Sagwal S, Freund E, Schmidt A. Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion. Adv Sci (Weinh). 2020;7:1903438 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d, s3a, s3b
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • flow cytometry; mouse; fig 5a
Azpilikueta A, Agorreta J, Labiano S, Pérez Gracia J, Sánchez Paulete A, Aznar M, et al. Successful Immunotherapy against a Transplantable Mouse Squamous Lung Carcinoma with Anti-PD-1 and Anti-CD137 Monoclonal Antibodies. J Thorac Oncol. 2016;11:524-36 pubmed publisher
Zhou J, Yang Q, Lu L, Tuo Z, Shou Z, Cheng J. PLK1 Inhibition Induces Immunogenic Cell Death and Enhances Immunity against NSCLC. Int J Med Sci. 2021;18:3516-3525 pubmed publisher
Wang X, Cai J, Lin B, Ma M, Tao Y, Zhou Y, et al. GPR34-mediated sensing of lysophosphatidylserine released by apoptotic neutrophils activates type 3 innate lymphoid cells to mediate tissue repair. Immunity. 2021;54:1123-1136.e8 pubmed publisher
Dikiy S, Li J, Bai L, Jiang M, Janke L, Zong X, et al. A distal Foxp3 enhancer enables interleukin-2 dependent thymic Treg cell lineage commitment for robust immune tolerance. Immunity. 2021;54:931-946.e11 pubmed publisher
Ringel A, Drijvers J, Baker G, Catozzi A, García Cañaveras J, Gassaway B, et al. Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity. Cell. 2020;183:1848-1866.e26 pubmed publisher
Ma X, Zou F, Yu F, Li R, Yuan Y, Zhang Y, et al. Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses. Immunity. 2020;53:1315-1330.e9 pubmed publisher
Jiang L, Wang Y, Zhao J, Uehara M, Hou Q, Kasinath V, et al. Direct Tumor Killing and Immunotherapy through Anti-SerpinB9 Therapy. Cell. 2020;183:1219-1233.e18 pubmed publisher
Tuttle K, Waugh K, Araya P, Minter R, Orlicky D, Ludwig M, et al. JAK1 Inhibition Blocks Lethal Immune Hypersensitivity in a Mouse Model of Down Syndrome. Cell Rep. 2020;33:108407 pubmed publisher
Fuster J, Zuriaga M, Zorita V, MacLauchlan S, Polackal M, Viana Huete V, et al. TET2-Loss-of-Function-Driven Clonal Hematopoiesis Exacerbates Experimental Insulin Resistance in Aging and Obesity. Cell Rep. 2020;33:108326 pubmed publisher
Liu C, Tam S, Huang Y, Dubé P, Alhosh R, Girish N, et al. TNF Receptor 1 Promotes Early-Life Immunity and Protects against Colitis in Mice. Cell Rep. 2020;33:108275 pubmed publisher
Lissner M, Cumnock K, Davis N, Vilches Moure J, Basak P, Navarrete D, et al. Metabolic profiling during malaria reveals the role of the aryl hydrocarbon receptor in regulating kidney injury. elife. 2020;9: pubmed publisher
Bogie J, Grajchen E, Wouters E, Broux B, Stinissen P, Van Wijmeersch B, et al. CNS delivery of anti-CD52 antibodies modestly reduces disease severity in an animal model for multiple sclerosis. Ther Adv Chronic Dis. 2020;11:2040622320947378 pubmed publisher
Si Y, Tian Q, Zhao F, Kelly S, Shores L, Camacho D, et al. Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and TH17 responses. Sci Adv. 2020;6:eaba0995 pubmed publisher
Koivisto C, Parrish M, Bonala S, Ngoi S, Torres A, Gallagher J, et al. Evaluating the efficacy of enzalutamide and the development of resistance in a preclinical mouse model of type-I endometrial carcinoma. Neoplasia. 2020;22:484-496 pubmed publisher
Kim S, Bagadia P, Anderson D, Liu T, Huang X, Theisen D, et al. High Amount of Transcription Factor IRF8 Engages AP1-IRF Composite Elements in Enhancers to Direct Type 1 Conventional Dendritic Cell Identity. Immunity. 2020;53:759-774.e9 pubmed publisher
Gajdasik D, Gaspal F, Halford E, Fiancette R, Dutton E, Willis C, et al. Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues. Nat Commun. 2020;11:3421 pubmed publisher
Loo C, Gatchalian J, Liang Y, Leblanc M, Xie M, Ho J, et al. A Genome-wide CRISPR Screen Reveals a Role for the Non-canonical Nucleosome-Remodeling BAF Complex in Foxp3 Expression and Regulatory T Cell Function. Immunity. 2020;53:143-157.e8 pubmed publisher
Jing Y, Dai X, Yang L, Kang D, Jiang P, Li N, et al. STING couples with PI3K to regulate actin reorganization during BCR activation. Sci Adv. 2020;6:eaax9455 pubmed publisher
Ni J, Wang X, Stojanovic A, Zhang Q, Wincher M, Bühler L, et al. Single-Cell RNA Sequencing of Tumor-Infiltrating NK Cells Reveals that Inhibition of Transcription Factor HIF-1α Unleashes NK Cell Activity. Immunity. 2020;52:1075-1087.e8 pubmed publisher
Yu X, Chan H, Fisher H, Penfold C, Kim J, Inzhelevskaya T, et al. Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity. Cancer Cell. 2020;37:850-866.e7 pubmed publisher
Teijeira A, Garasa S, Gato M, Alfaro C, Miguéliz I, Cirella A, et al. CXCR1 and CXCR2 Chemokine Receptor Agonists Produced by Tumors Induce Neutrophil Extracellular Traps that Interfere with Immune Cytotoxicity. Immunity. 2020;52:856-871.e8 pubmed publisher
Varikuti S, Singh B, Volpedo G, Ahirwar D, Jha B, Saljoughian N, et al. Ibrutinib treatment inhibits breast cancer progression and metastasis by inducing conversion of myeloid-derived suppressor cells to dendritic cells. Br J Cancer. 2020;122:1005-1013 pubmed publisher
Aegerter H, Kulikauskaite J, Crotta S, Patel H, Kelly G, Hessel E, et al. Influenza-induced monocyte-derived alveolar macrophages confer prolonged antibacterial protection. Nat Immunol. 2020;21:145-157 pubmed publisher
Ge C, Monk I, Pizzolla A, Wang N, Bedford J, Stinear T, et al. Bystander Activation of Pulmonary Trm Cells Attenuates the Severity of Bacterial Pneumonia by Enhancing Neutrophil Recruitment. Cell Rep. 2019;29:4236-4244.e3 pubmed publisher
Schaftenaar F, Amersfoort J, Douna H, Kröner M, Foks A, Bot I, et al. Induction of HLA-A2 restricted CD8 T cell responses against ApoB100 peptides does not affect atherosclerosis in a humanized mouse model. Sci Rep. 2019;9:17391 pubmed publisher
Mao W, Ghasemzadeh A, Freeman Z, Obradovic A, Chaimowitz M, Nirschl T, et al. Immunogenicity of prostate cancer is augmented by BET bromodomain inhibition. J Immunother Cancer. 2019;7:277 pubmed publisher
Bradley K, Finsterbusch K, Schnepf D, Crotta S, Llorian M, Davidson S, et al. Microbiota-Driven Tonic Interferon Signals in Lung Stromal Cells Protect from Influenza Virus Infection. Cell Rep. 2019;28:245-256.e4 pubmed publisher
Bommareddy P, Lowe D, Kaufman H, Rabkin S, Saha D. Multi-parametric flow cytometry staining procedure for analyzing tumor-infiltrating immune cells following oncolytic herpes simplex virus immunotherapy in intracranial glioblastoma. J Biol Methods. 2019;6: pubmed
Matsuoka S, Hashimoto D, Kadowaki M, Ohigashi H, Hayase E, Yokoyama E, et al. The myeloid differentiation factor 88 signaling in donor T cells accelerates graft-versus-host disease. Haematologica. 2019;: pubmed publisher
Li S, Sen S, Schneider J, Xiong K, Nusbacher N, Moreno Huizar N, et al. Gut microbiota from high-risk men who have sex with men drive immune activation in gnotobiotic mice and in vitro HIV infection. PLoS Pathog. 2019;15:e1007611 pubmed publisher
van Vloten J, Santry L, McAusland T, Karimi K, McFadden G, Petrik J, et al. Quantifying Antigen-Specific T Cell Responses When Using Antigen-Agnostic Immunotherapies. Mol Ther Methods Clin Dev. 2019;13:154-166 pubmed publisher
Zhang L, Chen S, Guerrero Juarez C, Li F, Tong Y, Liang Y, et al. Age-Related Loss of Innate Immune Antimicrobial Function of Dermal Fat Is Mediated by Transforming Growth Factor Beta. Immunity. 2019;50:121-136.e5 pubmed publisher
Fowler A, Tang W, Young M, Mamidi A, Viramontes K, McCauley M, et al. Maternally Acquired Zika Antibodies Enhance Dengue Disease Severity in Mice. Cell Host Microbe. 2018;24:743-750.e5 pubmed publisher
Kyburz A, Fallegger A, Zhang X, Altobelli A, Artola Borán M, Borbet T, et al. Transmaternal Helicobacter pylori exposure reduces allergic airway inflammation in offspring through regulatory T cells. J Allergy Clin Immunol. 2019;143:1496-1512.e11 pubmed publisher
Lebedeva E, Bagaev A, Pichugin A, Chulkina M, Lysenko A, Tutykhina I, et al. The differences in immunoadjuvant mechanisms of TLR3 and TLR4 agonists on the level of antigen-presenting cells during immunization with recombinant adenovirus vector. BMC Immunol. 2018;19:26 pubmed publisher
Urata S, Kenyon E, Nayak D, Cubitt B, Kurosaki Y, Yasuda J, et al. BST-2 controls T cell proliferation and exhaustion by shaping the early distribution of a persistent viral infection. PLoS Pathog. 2018;14:e1007172 pubmed publisher
Lino A, Dang V, Lampropoulou V, Welle A, Joedicke J, Pohar J, et al. LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma Cells. Immunity. 2018;49:120-133.e9 pubmed publisher
Moore M, Blachere N, Fak J, Park C, Sawicka K, Parveen S, et al. ZFP36 RNA-binding proteins restrain T cell activation and anti-viral immunity. elife. 2018;7: pubmed publisher
Wang D, Diao H, Getzler A, Rogal W, Frederick M, Milner J, et al. The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation. Immunity. 2018;48:659-674.e6 pubmed publisher
Herndler Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, et al. KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity. 2018;48:716-729.e8 pubmed publisher
Hiramoto T, Li L, Funk S, Hirata R, Russell D. Nuclease-free Adeno-Associated Virus-Mediated Il2rg Gene Editing in X-SCID Mice. Mol Ther. 2018;26:1255-1265 pubmed publisher
Zhu D, Tan J, Maleken A, Muljadi R, Chan S, Lau S, et al. Human amnion cells reverse acute and chronic pulmonary damage in experimental neonatal lung injury. Stem Cell Res Ther. 2017;8:257 pubmed publisher
Jenkins R, Aref A, Lizotte P, Ivanova E, Stinson S, Zhou C, et al. Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids. Cancer Discov. 2018;8:196-215 pubmed publisher
Soukup K, Halfmann A, Dillinger B, Poyer F, Martin K, Blauensteiner B, et al. Loss of MAPK-activated protein kinase 2 enables potent dendritic cell-driven anti-tumour T cell response. Sci Rep. 2017;7:11746 pubmed publisher
Cosway E, LUCAS B, James K, Parnell S, Carvalho Gaspar M, White A, et al. Redefining thymus medulla specialization for central tolerance. J Exp Med. 2017;214:3183-3195 pubmed publisher
Saha D, Martuza R, Rabkin S. Macrophage Polarization Contributes to Glioblastoma Eradication by Combination Immunovirotherapy and Immune Checkpoint Blockade. Cancer Cell. 2017;32:253-267.e5 pubmed publisher
Kyburz A, Urban S, Altobelli A, Floess S, Huehn J, Cover T, et al. Helicobacter pylori and its secreted immunomodulator VacA protect against anaphylaxis in experimental models of food allergy. Clin Exp Allergy. 2017;47:1331-1341 pubmed publisher
Telford W, Stickland L, Koschorreck M. Ultraviolet 320 nm laser excitation for flow cytometry. Cytometry A. 2017;91:314-325 pubmed publisher
Rodriguez Ruiz M, Rodríguez I, Garasa S, Barbés B, Solorzano J, Perez Gracia J, et al. Abscopal Effects of Radiotherapy Are Enhanced by Combined Immunostimulatory mAbs and Are Dependent on CD8 T Cells and Crosspriming. Cancer Res. 2016;76:5994-6005 pubmed
Wang C, Liu X, Li Z, Chai Y, Jiang Y, Wang Q, et al. CD8(+)NKT-like cells regulate the immune response by killing antigen-bearing DCs. Sci Rep. 2015;5:14124 pubmed publisher
Mairhofer D, Ortner D, Tripp C, Schaffenrath S, Fleming V, Heger L, et al. Impaired gp100-Specific CD8(+) T-Cell Responses in the Presence of Myeloid-Derived Suppressor Cells in a Spontaneous Mouse Melanoma Model. J Invest Dermatol. 2015;135:2785-2793 pubmed publisher
Cui L, Osada K, Imaizumi A, Kataoka K, Nakano K. Feasibility of a subcutaneously administered block/homo-mixed polyplex micelle as a carrier for DNA vaccination in a mouse tumor model. J Control Release. 2015;206:220-31 pubmed publisher
Wang T, Zhan X, Bu C, Lyon S, Pratt D, Hildebrand S, et al. Real-time resolution of point mutations that cause phenovariance in mice. Proc Natl Acad Sci U S A. 2015;112:E440-9 pubmed publisher
del Rio M, Cote Sierra J, Rodriguez Barbosa J. Flt3L-mobilized dendritic cells bearing H2-Kbm1 apoptotic cells do not induce cross-tolerance to CD8+ T cells across a class I MHC mismatched barrier. Transpl Int. 2011;24:501-13 pubmed publisher
Apte S, Groves P, Olver S, Baz A, Doolan D, Kelso A, et al. IFN-gamma inhibits IL-4-induced type 2 cytokine expression by CD8 T cells in vivo and modulates the anti-tumor response. J Immunol. 2010;185:998-1004 pubmed publisher
Bankoti J, Burnett A, Navarro S, Miller A, Rase B, Shepherd D. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010;115:422-34 pubmed publisher
Medyouf H, Gao X, Armstrong F, Gusscott S, Liu Q, Gedman A, et al. Acute T-cell leukemias remain dependent on Notch signaling despite PTEN and INK4A/ARF loss. Blood. 2010;115:1175-84 pubmed publisher
Todd D, McHeyzer Williams L, Kowal C, Lee A, Volpe B, Diamond B, et al. XBP1 governs late events in plasma cell differentiation and is not required for antigen-specific memory B cell development. J Exp Med. 2009;206:2151-9 pubmed publisher
Riedl P, Wieland A, Lamberth K, Buus S, Lemonnier F, Reifenberg K, et al. Elimination of immunodominant epitopes from multispecific DNA-based vaccines allows induction of CD8 T cells that have a striking antiviral potential. J Immunol. 2009;183:370-80 pubmed publisher
Lee C, Chiang Y, Chang S, Chong C, Cheng B, Roffler S. Tumor-localized ligation of CD3 and CD28 with systemic regulatory T-cell depletion induces potent innate and adaptive antitumor responses. Clin Cancer Res. 2009;15:2756-66 pubmed publisher
Britschgi M, Link A, Lissandrin T, Luther S. Dynamic modulation of CCR7 expression and function on naive T lymphocytes in vivo. J Immunol. 2008;181:7681-8 pubmed
Kingeter L, Schaefer B. Loss of protein kinase C theta, Bcl10, or Malt1 selectively impairs proliferation and NF-kappa B activation in the CD4+ T cell subset. J Immunol. 2008;181:6244-54 pubmed
Geiben Lynn R, Greenland J, Frimpong Boateng K, Van Rooijen N, Hovav A, Letvin N. CD4+ T lymphocytes mediate in vivo clearance of plasmid DNA vaccine antigen expression and potentiate CD8+ T-cell immune responses. Blood. 2008;112:4585-90 pubmed publisher
Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Andrews D, Andoniou C, Fleming P, Smyth M, Degli Esposti M. The early kinetics of cytomegalovirus-specific CD8+ T-cell responses are not affected by antigen load or the absence of perforin or gamma interferon. J Virol. 2008;82:4931-7 pubmed publisher
Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
Rasmussen J, Cello J, Gil H, Forestal C, Furie M, Thanassi D, et al. Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis. Infect Immun. 2006;74:6590-8 pubmed
Kao C, Daniels M, Jameson S. Loss of CD8 and TCR binding to Class I MHC ligands following T cell activation. Int Immunol. 2005;17:1607-17 pubmed
Ko S, Ko H, Chang W, Park S, Kweon M, Kang C. alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005;175:3309-17 pubmed
product information
Antigen :
CD8a
Apps. Abbrev. :
Flow Cytometry
Cat # :
100752
Clone :
53-6.7
Item :
Brilliant Violet 510™ anti-mouse CD8a
Isotype :
Rat IgG2a, κ
Other Names :
T8, Lyt2, Ly-2
Size :
50 μg
Price (USD) :
242 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV510
Immunogen :
Mouse thymus or spleen
Application Notes :
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes 3 . The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a + cells. Additional reported applications (for the relevant formats) include: immunoprecipitation 1,3 , in vivo and in vitro cell depletion 2,10,15 , inhibition of CD8 T cell proliferation 3 , blocking of cytotoxicity 3,4 , immunohistochemical staining 5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX) 29,30 . Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA