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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP anti-mouse CD8a
catalog :
100732
quantity :
100 μg
price :
245 USD
clonality :
monoclonal
host :
rat
conjugate :
PerCP
clone name :
53-6.7
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig e8a
Wan X, Zinselmeyer B, Zakharov P, Vomund A, Taniguchi R, Santambrogio L, et al. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Nature. 2018;560:107-111 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5a
Prado C, Gaiazzi M, Gonzalez H, Ugalde V, Figueroa A, Osorio Barrios F, et al. Dopaminergic Stimulation of Myeloid Antigen-Presenting Cells Attenuates Signal Transducer and Activator of Transcription 3-Activation Favouring the Development of Experimental Autoimmune Encephalomyelitis. Front Immunol. 2018;9:571 pubmed publisher
  • flow cytometry; mouse; loading ...
Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
  • flow cytometry; mouse
Olguín J, Fernández J, Salinas N, Juárez I, Rodriguez Sosa M, Campuzano J, et al. Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated Th1 immune response. Microbes Infect. 2015;17:586-95 pubmed publisher
Burzynski L, Humphry M, Pyrillou K, Wiggins K, Chan J, Figg N, et al. The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1α by Thrombin. Immunity. 2019;50:1033-1042.e6 pubmed publisher
Chen S, Fang L, Guo W, Zhou Y, Yu G, Li W, et al. Control of Treg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells. Nat Commun. 2018;9:5298 pubmed publisher
Nakamura Ishizu A, Matsumura T, Stumpf P, Umemoto T, Takizawa H, Takihara Y, et al. Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation. Cell Rep. 2018;25:1772-1785.e6 pubmed publisher
Lino A, Dang V, Lampropoulou V, Welle A, Joedicke J, Pohar J, et al. LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma Cells. Immunity. 2018;49:120-133.e9 pubmed publisher
Herndler Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, et al. KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity. 2018;48:716-729.e8 pubmed publisher
Palacios Arreola M, Nava Castro K, Río Araiza V, Pérez Sánchez N, Morales Montor J. A single neonatal administration of Bisphenol A induces higher tumour weight associated to changes in tumour microenvironment in the adulthood. Sci Rep. 2017;7:10573 pubmed publisher
Castiglioni P, Hartley M, Rossi M, Prevel F, Desponds C, Utzschneider D, et al. Exacerbated Leishmaniasis Caused by a Viral Endosymbiont can be Prevented by Immunization with Its Viral Capsid. PLoS Negl Trop Dis. 2017;11:e0005240 pubmed publisher
Mier Aguilar C, Cashman K, Raman C, Soldevila G. CD5-CK2 Signaling Modulates Erk Activation and Thymocyte Survival. PLoS ONE. 2016;11:e0168155 pubmed publisher
Clement M, Marsden M, Stacey M, Abdul Karim J, Gimeno Brias S, Costa Bento D, et al. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016;12:e1006050 pubmed publisher
Wang Y, Jiang X, Zhu J, Dan Yue -, Zhang X, Wang X, et al. IL-21/IL-21R signaling suppresses intestinal inflammation induced by DSS through regulation of Th responses in lamina propria in mice. Sci Rep. 2016;6:31881 pubmed publisher
Ben Shaanan T, Azulay Debby H, Dubovik T, Starosvetsky E, Korin B, Schiller M, et al. Activation of the reward system boosts innate and adaptive immunity. Nat Med. 2016;22:940-4 pubmed publisher
Sood S, Brownlie R, Garcia C, Cowan G, Salmond R, Sakaguchi S, et al. Loss of the Protein Tyrosine Phosphatase PTPN22 Reduces Mannan-Induced Autoimmune Arthritis in SKG Mice. J Immunol. 2016;197:429-40 pubmed publisher
Li X, Wang X, Thompson C, Park S, Park W, Lee J. Preclinical Efficacy of Clumping Factor A in Prevention of Staphylococcus aureus Infection. MBio. 2016;7:e02232-15 pubmed publisher
Choudhari R, Minero V, Menotti M, Pulito R, Brakebusch C, Compagno M, et al. Redundant and nonredundant roles for Cdc42 and Rac1 in lymphomas developed in NPM-ALK transgenic mice. Blood. 2016;127:1297-306 pubmed publisher
Leisegang M, Engels B, Schreiber K, Yew P, Kiyotani K, Idel C, et al. Eradication of Large Solid Tumors by Gene Therapy with a T-Cell Receptor Targeting a Single Cancer-Specific Point Mutation. Clin Cancer Res. 2016;22:2734-43 pubmed publisher
Ito C, Okuyama Dobashi K, Miyasaka T, Masuda C, Sato M, Kawano T, et al. CD8+ T Cells Mediate Female-Dominant IL-4 Production and Airway Inflammation in Allergic Asthma. PLoS ONE. 2015;10:e0140808 pubmed publisher
Martina M, Noel S, Saxena A, Bandapalle S, Majithia R, Jie C, et al. Double-Negative αβ T Cells Are Early Responders to AKI and Are Found in Human Kidney. J Am Soc Nephrol. 2016;27:1113-23 pubmed publisher
Jacque E, Schweighoffer E, Tybulewicz V, Ley S. BAFF activation of the ERK5 MAP kinase pathway regulates B cell survival. J Exp Med. 2015;212:883-92 pubmed publisher
Cui L, Osada K, Imaizumi A, Kataoka K, Nakano K. Feasibility of a subcutaneously administered block/homo-mixed polyplex micelle as a carrier for DNA vaccination in a mouse tumor model. J Control Release. 2015;206:220-31 pubmed publisher
Fan X, Wang X, Li N, Cui H, Hou B, Gao B, et al. Sortase A induces Th17-mediated and antibody-independent immunity to heterologous serotypes of group A streptococci. PLoS ONE. 2014;9:e107638 pubmed publisher
Jacque E, Schweighoffer E, Visekruna A, Papoutsopoulou S, Janzen J, Zillwood R, et al. IKK-induced NF-κB1 p105 proteolysis is critical for B cell antibody responses to T cell-dependent antigen. J Exp Med. 2014;211:2085-101 pubmed publisher
Collin R, Dugas V, Pelletier A, Chabot Roy G, Lesage S. The mouse idd2 locus is linked to the proportion of immunoregulatory double-negative T cells, a trait associated with autoimmune diabetes resistance. J Immunol. 2014;193:3503-12 pubmed publisher
Peng X, Keitany G, Vignali M, Chen L, Gibson C, Choi K, et al. Artesunate versus chloroquine infection-treatment-vaccination defines stage-specific immune responses associated with prolonged sterile protection against both pre-erythrocytic and erythrocytic Plasmodium yoelii infection. J Immunol. 2014;193:1268-77 pubmed publisher
Narusawa M, Inoue H, Sakamoto C, Matsumura Y, Takahashi A, Inoue T, et al. TLR7 ligand augments GM-CSF-initiated antitumor immunity through activation of plasmacytoid dendritic cells. Cancer Immunol Res. 2014;2:568-80 pubmed publisher
Brenndörfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol. 2014;192:1671-80 pubmed publisher
Byrne K, Zhang P, Steinberg S, Turk M. Autoimmune vitiligo does not require the ongoing priming of naive CD8 T cells for disease progression or associated protection against melanoma. J Immunol. 2014;192:1433-9 pubmed publisher
Sun Z, Xiao Z. 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) regulates CTL activation and memory programming. Biochem Biophys Res Commun. 2013;435:472-6 pubmed publisher
del Rio M, Cote Sierra J, Rodriguez Barbosa J. Flt3L-mobilized dendritic cells bearing H2-Kbm1 apoptotic cells do not induce cross-tolerance to CD8+ T cells across a class I MHC mismatched barrier. Transpl Int. 2011;24:501-13 pubmed publisher
Apte S, Groves P, Olver S, Baz A, Doolan D, Kelso A, et al. IFN-gamma inhibits IL-4-induced type 2 cytokine expression by CD8 T cells in vivo and modulates the anti-tumor response. J Immunol. 2010;185:998-1004 pubmed publisher
Bankoti J, Burnett A, Navarro S, Miller A, Rase B, Shepherd D. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010;115:422-34 pubmed publisher
Medyouf H, Gao X, Armstrong F, Gusscott S, Liu Q, Gedman A, et al. Acute T-cell leukemias remain dependent on Notch signaling despite PTEN and INK4A/ARF loss. Blood. 2010;115:1175-84 pubmed publisher
Todd D, McHeyzer Williams L, Kowal C, Lee A, Volpe B, Diamond B, et al. XBP1 governs late events in plasma cell differentiation and is not required for antigen-specific memory B cell development. J Exp Med. 2009;206:2151-9 pubmed publisher
Riedl P, Wieland A, Lamberth K, Buus S, Lemonnier F, Reifenberg K, et al. Elimination of immunodominant epitopes from multispecific DNA-based vaccines allows induction of CD8 T cells that have a striking antiviral potential. J Immunol. 2009;183:370-80 pubmed publisher
Lee C, Chiang Y, Chang S, Chong C, Cheng B, Roffler S. Tumor-localized ligation of CD3 and CD28 with systemic regulatory T-cell depletion induces potent innate and adaptive antitumor responses. Clin Cancer Res. 2009;15:2756-66 pubmed publisher
Britschgi M, Link A, Lissandrin T, Luther S. Dynamic modulation of CCR7 expression and function on naive T lymphocytes in vivo. J Immunol. 2008;181:7681-8 pubmed
Kingeter L, Schaefer B. Loss of protein kinase C theta, Bcl10, or Malt1 selectively impairs proliferation and NF-kappa B activation in the CD4+ T cell subset. J Immunol. 2008;181:6244-54 pubmed
Geiben Lynn R, Greenland J, Frimpong Boateng K, Van Rooijen N, Hovav A, Letvin N. CD4+ T lymphocytes mediate in vivo clearance of plasmid DNA vaccine antigen expression and potentiate CD8+ T-cell immune responses. Blood. 2008;112:4585-90 pubmed publisher
Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Andrews D, Andoniou C, Fleming P, Smyth M, Degli Esposti M. The early kinetics of cytomegalovirus-specific CD8+ T-cell responses are not affected by antigen load or the absence of perforin or gamma interferon. J Virol. 2008;82:4931-7 pubmed publisher
Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
Rasmussen J, Cello J, Gil H, Forestal C, Furie M, Thanassi D, et al. Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis. Infect Immun. 2006;74:6590-8 pubmed
Kao C, Daniels M, Jameson S. Loss of CD8 and TCR binding to Class I MHC ligands following T cell activation. Int Immunol. 2005;17:1607-17 pubmed
Ko S, Ko H, Chang W, Park S, Kweon M, Kang C. alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005;175:3309-17 pubmed
product information
Antigen :
CD8a
Apps. Abbrev. :
Flow Cytometry
Cat # :
100732
Clone :
53-6.7
Item :
PerCP anti-mouse CD8a
Isotype :
Rat IgG2a, κ
Other Names :
T8, Lyt2, Ly-2
Size :
100 μg
Price (USD) :
245 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PERCP
Immunogen :
Mouse thymus or spleen
Application Notes :
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes 3 . The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a + cells. Additional reported applications (for the relevant formats) include: immunoprecipitation 1,3 , in vivo and in vitro cell depletion 2,10,15 , inhibition of CD8 T cell proliferation 3 , blocking of cytotoxicity 3,4 , and immunohistochemical staining 5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 μm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA