LEAF™ Purified anti-mouse CD8a | BioLegend 100715 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse CD8a

catalog :

100715

quantity :

50 μg

price :

30 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

53-6.7

reactivity :

human, mouse

application :

immunohistochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments

citations: 161

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 1b	Qiu J, Villa M, Sanin D, Buck M, O Sullivan D, Ching R, et al. Acetate Promotes T Cell Effector Function during Glucose Restriction. Cell Rep. 2019;27:2063-2074.e5 pubmed publisher
flow cytometry; mouse; loading ...; fig s2a	Martins J, Andoniou C, Fleming P, Kuns R, Schuster I, Voigt V, et al. Strain-specific antibody therapy prevents cytomegalovirus reactivation after transplantation. Science. 2019;363:288-293 pubmed publisher
flow cytometry; mouse; loading ...; fig 1c	McLaren J, Clement M, Marsden M, Miners K, Llewellyn Lacey S, Grant E, et al. IL-33 Augments Virus-Specific Memory T Cell Inflation and Potentiates the Efficacy of an Attenuated Cytomegalovirus-Based Vaccine. J Immunol. 2019;202:943-955 pubmed publisher
flow cytometry; mouse; loading ...; fig s5b	Ruscetti M, Leibold J, Bott M, Fennell M, Kulick A, Salgado N, et al. NK cell-mediated cytotoxicity contributes to tumor control by a cytostatic drug combination. Science. 2018;362:1416-1422 pubmed publisher
flow cytometry; mouse; loading ...; fig s2a	Gubernatorova E, Gorshkova E, Namakanova O, Zvartsev R, Hidalgo J, Drutskaya M, et al. Non-redundant Functions of IL-6 Produced by Macrophages and Dendritic Cells in Allergic Airway Inflammation. Front Immunol. 2018;9:2718 pubmed publisher
flow cytometry; mouse; loading ...; fig s2a, s2b	Sato Y, Bolzenius J, Eteleeb A, Su X, Maher C, Sehn J, et al. CD4+ T cells induce rejection of urothelial tumors after immune checkpoint blockade. JCI Insight. 2018;3: pubmed publisher
flow cytometry; mouse; loading ...; fig 1c	Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
flow cytometry; mouse; loading ...; fig 4a	Williams G, Schonhoff A, Jurkuvenaite A, Thome A, Standaert D, Harms A. Targeting of the class II transactivator attenuates inflammation and neurodegeneration in an alpha-synuclein model of Parkinson's disease. J Neuroinflammation. 2018;15:244 pubmed publisher
flow cytometry; mouse; loading ...; fig 1h	Wang C, Oshima M, Sato D, Matsui H, Kubota S, Aoyama K, et al. Ezh2 loss propagates hypermethylation at T cell differentiation-regulating genes to promote leukemic transformation. J Clin Invest. 2018;128:3872-3886 pubmed publisher
flow cytometry; mouse; loading ...; fig e8a	Wan X, Zinselmeyer B, Zakharov P, Vomund A, Taniguchi R, Santambrogio L, et al. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Nature. 2018;560:107-111 pubmed publisher
flow cytometry; mouse; loading ...; fig 5b	Vendetti F, Karukonda P, Clump D, Teo T, Lalonde R, Nugent K, et al. ATR kinase inhibitor AZD6738 potentiates CD8+ T cell-dependent antitumor activity following radiation. J Clin Invest. 2018;128:3926-3940 pubmed publisher
flow cytometry; mouse; fig s3c	Napolitano A, van der Veen A, Bunyan M, Borg A, Frith D, Howell S, et al. Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+ Dendritic Cells at the Site of Infection. J Immunol. 2018;201:604-614 pubmed publisher
flow cytometry; mouse; loading ...; fig s2j	Ma C, Han M, Heinrich B, Fu Q, Zhang Q, Sandhu M, et al. Gut microbiome-mediated bile acid metabolism regulates liver cancer via NKT cells. Science. 2018;360: pubmed publisher
flow cytometry; mouse; loading ...; fig 3g	Jun H, Yu H, Gong J, Jiang J, Qiao X, Perkey E, et al. An immune-beige adipocyte communication via nicotinic acetylcholine receptor signaling. Nat Med. 2018;24:814-822 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig s2a	Pommier A, Anaparthy N, Memos N, Kelley Z, Gouronnec A, Yan R, et al. Unresolved endoplasmic reticulum stress engenders immune-resistant, latent pancreatic cancer metastases. Science. 2018;360: pubmed publisher
flow cytometry; mouse; loading ...; fig 5d	Stefani F, Eberstål S, Vergani S, Kristiansen T, Bengzon J. Low-dose irradiated mesenchymal stromal cells break tumor defensive properties in vivo. Int J Cancer. 2018;143:2200-2212 pubmed publisher
flow cytometry; mouse; loading ...; fig 1c	Takamori A, Nambu A, Sato K, Yamaguchi S, Matsuda K, Numata T, et al. IL-31 is crucial for induction of pruritus, but not inflammation, in contact hypersensitivity. Sci Rep. 2018;8:6639 pubmed publisher
flow cytometry; mouse; loading ...; fig s2c	Tanaka S, Pfleger C, Lai J, Roan F, Sun S, Ziegler S. KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners. Cell Rep. 2018;23:796-807 pubmed publisher
flow cytometry; mouse; loading ...; fig s11	Foerster F, Boegel S, Heck R, Pickert G, R ssel N, Rosigkeit S, et al. Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells. Oncoimmunology. 2018;7:e1409929 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 5c	Carrasco S, Hu S, Imai D, Kumar R, Sandusky G, Yang X, et al. Toll-like receptor 3 (TLR3) promotes the resolution of Chlamydia muridarum genital tract infection in congenic C57BL/6N mice. PLoS ONE. 2018;13:e0195165 pubmed publisher
flow cytometry; mouse; loading ...; fig s5a	Prado C, Gaiazzi M, Gonzalez H, Ugalde V, Figueroa A, Osorio Barrios F, et al. Dopaminergic Stimulation of Myeloid Antigen-Presenting Cells Attenuates Signal Transducer and Activator of Transcription 3-Activation Favouring the Development of Experimental Autoimmune Encephalomyelitis. Front Immunol. 2018;9:571 pubmed publisher
flow cytometry; mouse; loading ...; fig 2f	Lee J, Park J, Nam T, Seo S, Kim J, Lee H, et al. Differences between immunodeficient mice generated by classical gene targeting and CRISPR/Cas9-mediated gene knockout. Transgenic Res. 2018;27:241-251 pubmed publisher
immunohistochemistry - frozen section; mouse; 2.5 mg/ml; loading ...; fig s5c	Sun H, Lagarrigue F, Gingras A, Fan Z, Ley K, Ginsberg M. Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development. J Cell Biol. 2018;217:1453-1465 pubmed publisher
flow cytometry; mouse; loading ...; fig s4a	Tang C, Chang S, Paton A, Paton J, Gabrilovich D, Ploegh H, et al. Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization. J Cell Biol. 2018;217:1739-1755 pubmed publisher
flow cytometry; mouse; loading ...; fig s2	Lee Y, Lee J, Jang Y, Seo S, Chang J, Seong B. Non-specific Effect of Vaccines: Immediate Protection against Respiratory Syncytial Virus Infection by a Live Attenuated Influenza Vaccine. Front Microbiol. 2018;9:83 pubmed publisher
flow cytometry; mouse; loading ...; fig 1a	Fahl S, Coffey F, Kain L, Zarin P, Dunbrack R, Teyton L, et al. Role of a selecting ligand in shaping the murine γδ-TCR repertoire. Proc Natl Acad Sci U S A. 2018;115:1889-1894 pubmed publisher
flow cytometry; mouse; loading ...; fig 3c	Dejea C, Fathi P, Craig J, Boleij A, Taddese R, Geis A, et al. Patients with familial adenomatous polyposis harbor colonic biofilms containing tumorigenic bacteria. Science. 2018;359:592-597 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Doorduijn E, Sluijter M, Marijt K, Querido B, van der Burg S, van Hall T. T cells specific for a TAP-independent self-peptide remain naïve in tumor-bearing mice and are fully exploitable for therapy. Oncoimmunology. 2018;7:e1382793 pubmed publisher
flow cytometry; mouse; loading ...; fig 3b, 3e	Scott J, Lebratti T, Richner J, Jiang X, Fernandez E, Zhao H, et al. Cellular and Humoral Immunity Protect against Vaginal Zika Virus Infection in Mice. J Virol. 2018;92: pubmed publisher
flow cytometry; mouse; loading ...; fig 3b	Turner D, Goldklang M, Cvetkovski F, Paik D, Trischler J, Barahona J, et al. Biased Generation and In Situ Activation of Lung Tissue-Resident Memory CD4 T Cells in the Pathogenesis of Allergic Asthma. J Immunol. 2018;200:1561-1569 pubmed publisher
flow cytometry; mouse; loading ...; fig 3f	Pan D, Kobayashi A, Jiang P, Ferrari de Andrade L, Tay R, Luoma A, et al. A major chromatin regulator determines resistance of tumor cells to T cell-mediated killing. Science. 2018;359:770-775 pubmed publisher
flow cytometry; mouse; loading ...; fig 5a	Cowan J, Baik S, McCarthy N, Parnell S, White A, Jenkinson W, et al. Aire controls the recirculation of murine Foxp3+ regulatory T-cells back to the thymus. Eur J Immunol. 2018;48:844-854 pubmed publisher
flow cytometry; mouse; loading ...; fig 1k	Lynch J, Werder R, Loh Z, Sikder M, Curren B, Zhang V, et al. Plasmacytoid dendritic cells protect from viral bronchiolitis and asthma through semaphorin 4a-mediated T reg expansion. J Exp Med. 2018;215:537-557 pubmed publisher
flow cytometry; mouse; loading ...; fig 6a	Zhang Y, Khairallah C, Sheridan B, van der Velden A, Bliska J. CCR2+ Inflammatory Monocytes Are Recruited to Yersinia pseudotuberculosis Pyogranulomas and Dictate Adaptive Responses at the Expense of Innate Immunity during Oral Infection. Infect Immun. 2018;86: pubmed publisher
flow cytometry; mouse; loading ...; fig 4b	Matsuo K, Nagakubo D, Yamamoto S, Shigeta A, Tomida S, Fujita M, et al. CCL28-Deficient Mice Have Reduced IgA Antibody-Secreting Cells and an Altered Microbiota in the Colon. J Immunol. 2018;200:800-809 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 3b	Medaglia C, Giladi A, Stoler Barak L, De Giovanni M, Salame T, Biram A, et al. Spatial reconstruction of immune niches by combining photoactivatable reporters and scRNA-seq. Science. 2017;358:1622-1626 pubmed publisher
flow cytometry; mouse; loading ...; fig 2b	Pedros C, Canonigo Balancio A, Kong K, Altman A. Requirement of Treg-intrinsic CTLA4/PKCη signaling pathway for suppressing tumor immunity. JCI Insight. 2017;2: pubmed publisher
flow cytometry; mouse; loading ...; fig s5d	Wallrapp A, Riesenfeld S, Burkett P, Abdulnour R, Nyman J, Dionne D, et al. The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation. Nature. 2017;549:351-356 pubmed publisher
flow cytometry; mouse; 1:400; loading ...; fig s3a	Hasan Z, Koizumi S, Sasaki D, Yamada H, Arakaki N, Fujihara Y, et al. JunB is essential for IL-23-dependent pathogenicity of Th17 cells. Nat Commun. 2017;8:15628 pubmed publisher
flow cytometry; mouse; fig 1d	Jinnohara T, Kanaya T, Hase K, Sakakibara S, Kato T, Tachibana N, et al. IL-22BP dictates characteristics of Peyer's patch follicle-associated epithelium for antigen uptake. J Exp Med. 2017;214:1607-1618 pubmed publisher
flow cytometry; mouse; loading ...; fig 4e	Daley D, Mani V, Mohan N, Akkad N, Pandian G, Savadkar S, et al. NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma. J Exp Med. 2017;214:1711-1724 pubmed publisher
flow cytometry; mouse; loading ...; fig 1b	Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
flow cytometry; mouse; loading ...; fig 3b	Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
flow cytometry; mouse; loading ...; fig s1g	Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, et al. BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J Exp Med. 2017;214:1313-1331 pubmed publisher
flow cytometry; mouse; loading ...	Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
flow cytometry; mouse; loading ...; fig 2a	González Pérez G, Lamousé Smith E. Gastrointestinal Microbiome Dysbiosis in Infant Mice Alters Peripheral CD8+ T Cell Receptor Signaling. Front Immunol. 2017;8:265 pubmed publisher
flow cytometry; mouse; fig 6e	Obeid S, Wankell M, Charrez B, Sternberg J, Kreuter R, Esmaili S, et al. Adiponectin confers protection from acute colitis and restricts a B cell immune response. J Biol Chem. 2017;292:6569-6582 pubmed publisher
flow cytometry; mouse; loading ...; fig s2h	Ramos G, van den Berg A, Nunes Silva V, Weirather J, Peters L, Burkard M, et al. Myocardial aging as a T-cell-mediated phenomenon. Proc Natl Acad Sci U S A. 2017;114:E2420-E2429 pubmed publisher
flow cytometry; mouse; fig 5c	Oh J, Oh D, Jung H, Lee H. A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract. Proc Natl Acad Sci U S A. 2017;114:E1188-E1195 pubmed publisher
flow cytometry; mouse; loading ...; fig 4b	Yanagita T, Murata Y, Tanaka D, Motegi S, Arai E, Daniwijaya E, et al. Anti-SIRPα antibodies as a potential new tool for cancer immunotherapy. JCI Insight. 2017;2:e89140 pubmed publisher
flow cytometry; mouse; loading ...; fig 1g	Chamoto K, Chowdhury P, Kumar A, Sonomura K, Matsuda F, Fagarasan S, et al. Mitochondrial activation chemicals synergize with surface receptor PD-1 blockade for T cell-dependent antitumor activity. Proc Natl Acad Sci U S A. 2017;114:E761-E770 pubmed publisher
flow cytometry; mouse; loading ...; fig s6a	van der Weyden L, Arends M, Campbell A, Bald T, Wardle Jones H, Griggs N, et al. Genome-wide in vivo screen identifies novel host regulators of metastatic colonization. Nature. 2017;541:233-236 pubmed publisher
flow cytometry; mouse; loading ...; fig 2	Engler J, Kursawe N, Solano M, Patas K, Wehrmann S, Heckmann N, et al. Glucocorticoid receptor in T cells mediates protection from autoimmunity in pregnancy. Proc Natl Acad Sci U S A. 2017;114:E181-E190 pubmed publisher
blocking or activating experiments; mouse; loading ...; fig 6a	Li M, Li Z, Yao Y, Jin W, Wood K, Liu Q, et al. Astrocyte-derived interleukin-15 exacerbates ischemic brain injury via propagation of cellular immunity. Proc Natl Acad Sci U S A. 2017;114:E396-E405 pubmed publisher
flow cytometry; mouse; loading ...; fig 2a	Wei Y, Lu C, Chen J, Cui G, Wang L, Yu T, et al. High salt diet stimulates gut Th17 response and exacerbates TNBS-induced colitis in mice. Oncotarget. 2017;8:70-82 pubmed publisher
flow cytometry; mouse; loading ...; fig 1e	Schneider C, Nobs S, Heer A, Hirsch E, Penninger J, Siggs O, et al. Frontline Science: Coincidental null mutation of Csf2rÎ± in a colony of PI3KÎ³-/- mice causes alveolar macrophage deficiency and fatal respiratory viral infection. J Leukoc Biol. 2017;101:367-376 pubmed publisher
flow cytometry; mouse; loading ...; fig 1b	Swanson P, Hart G, Russo M, Nayak D, Yazew T, Pena M, et al. CD8+ T Cells Induce Fatal Brainstem Pathology during Cerebral Malaria via Luminal Antigen-Specific Engagement of Brain Vasculature. PLoS Pathog. 2016;12:e1006022 pubmed publisher
flow cytometry; mouse; loading ...; fig s3c	Hammer A, Yang G, Friedrich J, Kovacs A, Lee D, Grave K, et al. Role of the receptor Mas in macrophage-mediated inflammation in vivo. Proc Natl Acad Sci U S A. 2016;113:14109-14114 pubmed
flow cytometry; mouse; loading ...; fig s2	Park K, Mikulski Z, Seo G, Andreyev A, Marcovecchio P, Blatchley A, et al. The transcription factor NR4A3 controls CD103+ dendritic cell migration. J Clin Invest. 2016;126:4603-4615 pubmed publisher
flow cytometry; mouse; 1:1000; loading ...; fig 4c	Hirako I, Ataide M, Faustino L, Assis P, Sorensen E, Ueta H, et al. Splenic differentiation and emergence of CCR5+CXCL9+CXCL10+ monocyte-derived dendritic cells in the brain during cerebral malaria. Nat Commun. 2016;7:13277 pubmed publisher
flow cytometry; mouse; loading ...; fig 4b	Kirschbaum K, Sonner J, Zeller M, Deumelandt K, Bode J, Sharma R, et al. In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis. Proc Natl Acad Sci U S A. 2016;113:13227-13232 pubmed
flow cytometry; mouse; loading ...; fig 1c	Jirmo A, Daluege K, Happle C, Albrecht M, Dittrich A, Busse M, et al. IL-27 Is Essential for Suppression of Experimental Allergic Asthma by the TLR7/8 Agonist R848 (Resiquimod). J Immunol. 2016;197:4219-4227 pubmed
flow cytometry; mouse; fig 6	Serr I, FÃ¼rst R, Ott V, Scherm M, Nikolaev A, GÃ¶kmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
flow cytometry; mouse; 1:400; loading ...; fig 2a	Starobinets H, Ye J, Broz M, Barry K, Goldsmith J, Marsh T, et al. Antitumor adaptive immunity remains intact following inhibition of autophagy and antimalarial treatment. J Clin Invest. 2016;126:4417-4429 pubmed publisher
flow cytometry; mouse; 1:50; loading ...; fig s2e	Kimura T, Nada S, Takegahara N, Okuno T, Nojima S, Kang S, et al. Polarization of M2 macrophages requires Lamtor1 that integrates cytokine and amino-acid signals. Nat Commun. 2016;7:13130 pubmed publisher
flow cytometry; mouse; fig 2	Huang M, Zhang W, Guo J, Wei X, Phiwpan K, Zhang J, et al. Improved Transgenic Mouse Model for Studying HLA Class I Antigen Presentation. Sci Rep. 2016;6:33612 pubmed publisher
flow cytometry; human; 1:100; loading ...	Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
flow cytometry; mouse; fig 6	Ushiki T, Huntington N, Glaser S, Kiu H, Georgiou A, Zhang J, et al. Rapid Inflammation in Mice Lacking Both SOCS1 and SOCS3 in Hematopoietic Cells. PLoS ONE. 2016;11:e0162111 pubmed publisher
flow cytometry; mouse; fig s1	Kong S, Yang Y, Xu Y, Wang Y, Zhang Y, Melo Cardenas J, et al. Endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 controls B-cell immunity through degradation of the death receptor CD95/Fas. Proc Natl Acad Sci U S A. 2016;113:10394-9 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 8c	Chopra M, Biehl M, Steinfatt T, Brandl A, Kums J, Amich J, et al. Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med. 2016;213:1881-900 pubmed publisher
flow cytometry; mouse; loading ...; fig 4g	Barin J, Talor M, Schaub J, Diny N, Hou X, Hoyer M, et al. Collaborative Interferon-? and Interleukin-17 Signaling Protects the Oral Mucosa from Staphylococcus aureus. Am J Pathol. 2016;186:2337-52 pubmed publisher
flow cytometry; mouse; fig 6a	Aryal B, Rotllan N, Araldi E, Ramírez C, He S, Chousterman B, et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. 2016;7:12313 pubmed publisher
flow cytometry; human; fig 4	Di Scala M, Otano I, Gil Farina I, Vanrell L, Hommel M, Olague C, et al. Complementary Effects of Interleukin-15 and Alpha Interferon Induce Immunity in Hepatitis B Virus Transgenic Mice. J Virol. 2016;90:8563-74 pubmed publisher
flow cytometry; mouse; loading ...; fig s6b	Xiao Y, Tang J, Guo H, Zhao Y, Tang R, Ouyang S, et al. Targeting CBLB as a potential therapeutic approach for disseminated candidiasis. Nat Med. 2016;22:906-14 pubmed publisher
flow cytometry; mouse; loading ...; fig 1b	Ibiza S, García Cassani B, Ribeiro H, Carvalho T, Almeida L, Marques R, et al. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence. Nature. 2016;535:440-443 pubmed publisher
flow cytometry; mouse; 1:300; loading ...; fig s4b	Riedel A, Shorthouse D, Haas L, Hall B, Shields J. Tumor-induced stromal reprogramming drives lymph node transformation. Nat Immunol. 2016;17:1118-27 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig s1	Göbel K, Pankratz S, Asaridou C, Herrmann A, Bittner S, Merker M, et al. Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells. Nat Commun. 2016;7:11626 pubmed publisher
flow cytometry; mouse; loading ...; fig 1b	Qualai J, Li L, Cantero J, Tarrats A, Fernández M, Sumoy L, et al. Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential. PLoS ONE. 2016;11:e0154253 pubmed publisher
flow cytometry; mouse; fig 5	Li Y, Nishikawa T, Kaneda Y. Platelet-cytokine Complex Suppresses Tumour Growth by Exploiting Intratumoural Thrombin-dependent Platelet Aggregation. Sci Rep. 2016;6:25077 pubmed publisher
flow cytometry; mouse; 1:100; fig s5	Li Z, Hodgkinson T, Gothard E, Boroumand S, Lamb R, Cummins I, et al. Epidermal Notch1 recruits RORγ(+) group 3 innate lymphoid cells to orchestrate normal skin repair. Nat Commun. 2016;7:11394 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 1a	O Leary C, Riling C, Spruce L, Ding H, Kumar S, Deng G, et al. Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells. Nat Commun. 2016;7:11226 pubmed publisher
flow cytometry; mouse	Mall C, Sckisel G, Proia D, Mirsoian A, Grossenbacher S, Pai C, et al. Repeated PD-1/PD-L1 monoclonal antibody administration induces fatal xenogeneic hypersensitivity reactions in a murine model of breast cancer. Oncoimmunology. 2016;5:e1075114 pubmed
flow cytometry; mouse; fig s1	Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
flow cytometry; mouse; fig 2	Apostolidis S, RodrÃguez RodrÃguez N, SuÃ¡rez Fueyo A, Dioufa N, Ozcan E, CrispÃn J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
flow cytometry; mouse; loading ...; fig 7d	McFarland B, Marks M, Rowse A, Fehling S, Gerigk M, Qin H, et al. Loss of SOCS3 in myeloid cells prolongs survival in a syngeneic model of glioma. Oncotarget. 2016;7:20621-35 pubmed publisher
flow cytometry; mouse; loading ...	Flach A, Litke T, Strauss J, Haberl M, GÃ³mez C, Reindl M, et al. Autoantibody-boosted T-cell reactivation in the target organ triggers manifestation of autoimmune CNS disease. Proc Natl Acad Sci U S A. 2016;113:3323-8 pubmed publisher
flow cytometry; mouse; fig 5	Tagliamonte M, Petrizzo A, Napolitano M, Luciano A, Rea D, Barbieri A, et al. A novel multi-drug metronomic chemotherapy significantly delays tumor growth in mice. J Transl Med. 2016;14:58 pubmed publisher
flow cytometry; mouse; fig 5	Foy S, Sennino B, dela Cruz T, Cote J, Gordon E, Kemp F, et al. Poxvirus-Based Active Immunotherapy with PD-1 and LAG-3 Dual Immune Checkpoint Inhibition Overcomes Compensatory Immune Regulation, Yielding Complete Tumor Regression in Mice. PLoS ONE. 2016;11:e0150084 pubmed publisher
flow cytometry; mouse; fig 1	Pelly V, Kannan Y, Coomes S, Entwistle L, RÃ¼ckerl D, Seddon B, et al. IL-4-producing ILC2s are required for the differentiation of TH2 cells following Heligmosomoides polygyrus infection. Mucosal Immunol. 2016;9:1407-1417 pubmed publisher
flow cytometry; mouse; fig 5a	Azpilikueta A, Agorreta J, Labiano S, PÃ©rez Gracia J, SÃ¡nchez Paulete A, Aznar M, et al. Successful Immunotherapy against a Transplantable Mouse Squamous Lung Carcinoma with Anti-PD-1 and Anti-CD137 Monoclonal Antibodies. J Thorac Oncol. 2016;11:524-36 pubmed publisher
flow cytometry; mouse; fig 2	Polansky J, Bahri R, Divivier M, Duitman E, Vock C, Goyeneche Patino D, et al. High dose CD11c-driven IL15 is sufficient to drive NK cell maturation and anti-tumor activity in a trans-presentation independent manner. Sci Rep. 2016;6:19699 pubmed publisher
flow cytometry; mouse; fig s5	Catarinella M, Monestiroli A, Escobar G, Fiocchi A, Tran N, Aiolfi R, et al. IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment. EMBO Mol Med. 2016;8:155-70 pubmed publisher
flow cytometry; mouse	LasigliÃ¨ D, Boero S, Bauer I, Morando S, Damonte P, Cea M, et al. Sirt6 regulates dendritic cell differentiation, maturation, and function. Aging (Albany NY). 2016;8:34-49 pubmed
flow cytometry; mouse; fig 4	Yasuma K, Yasunaga J, Takemoto K, Sugata K, Mitobe Y, Takenouchi N, et al. HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT). PLoS Pathog. 2016;12:e1005372 pubmed publisher
flow cytometry; mouse; loading ...	Jabara H, Boyden S, Chou J, Ramesh N, Massaad M, Benson H, et al. A missense mutation in TFRC, encoding transferrin receptor 1, causes combined immunodeficiency. Nat Genet. 2016;48:74-8 pubmed publisher
flow cytometry; mouse; fig 5	Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, et al. Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection. Eur J Immunol. 2016;46:464-79 pubmed publisher
flow cytometry; mouse; fig 4	Smith K, Filbey K, Reynolds L, Hewitson J, Harcus Y, Boon L, et al. Low-level regulatory T-cell activity is essential for functional type-2 effector immunity to expel gastrointestinal helminths. Mucosal Immunol. 2016;9:428-43 pubmed publisher
flow cytometry; mouse; fig 2	Kim J, Phan T, Nguyen V, Dinh Vu H, Zheng J, Yun M, et al. Salmonella typhimurium Suppresses Tumor Growth via the Pro-Inflammatory Cytokine Interleukin-1Î². Theranostics. 2015;5:1328-42 pubmed publisher
flow cytometry; mouse; fig 1 immunohistochemistry; mouse; fig 1	Adachi T, Kobayashi T, Sugihara E, Yamada T, Ikuta K, Pittaluga S, et al. Hair follicle-derived IL-7 and IL-15 mediate skin-resident memory T cell homeostasis and lymphoma. Nat Med. 2015;21:1272-9 pubmed publisher
flow cytometry; mouse	Jones D, Wilmore J, Allman D. Cellular Dynamics of Memory B Cell Populations: IgM+ and IgG+ Memory B Cells Persist Indefinitely as Quiescent Cells. J Immunol. 2015;195:4753-9 pubmed publisher
flow cytometry; human	Sewald X, Ladinsky M, Uchil P, Beloor J, Pi R, Herrmann C, et al. Retroviruses use CD169-mediated trans-infection of permissive lymphocytes to establish infection. Science. 2015;350:563-567 pubmed publisher
flow cytometry; mouse; fig s7	McCormack R, de Armas L, Shiratsuchi M, Fiorentino D, Olsson M, Lichtenheld M, et al. Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. elife. 2015;4: pubmed publisher
immunohistochemistry - frozen section; mouse; fig 4	Yang H, Yamazaki T, Pietrocola F, Zhou H, Zitvogel L, Ma Y, et al. STAT3 Inhibition Enhances the Therapeutic Efficacy of Immunogenic Chemotherapy by Stimulating Type 1 Interferon Production by Cancer Cells. Cancer Res. 2015;75:3812-22 pubmed publisher
flow cytometry; mouse; loading ...; fig 1c	Ngiow S, Young A, Jacquelot N, Yamazaki T, Enot D, Zitvogel L, et al. A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1. Cancer Res. 2015;75:3800-11 pubmed publisher
flow cytometry; mouse; loading ...; fig s1	Kim M, Taparowsky E, Kim C. Retinoic Acid Differentially Regulates the Migration of Innate Lymphoid Cell Subsets to the Gut. Immunity. 2015;43:107-19 pubmed publisher
flow cytometry; mouse; loading ...; fig 2a	Herz J, Johnson K, McGavern D. Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells. J Exp Med. 2015;212:1153-69 pubmed publisher
flow cytometry; mouse; 10 ug/ml; fig 7a	Sasaki K, Takada K, Ohte Y, Kondo H, Sorimachi H, Tanaka K, et al. Thymoproteasomes produce unique peptide motifs for positive selection of CD8(+) T cells. Nat Commun. 2015;6:7484 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 9	Onishi S, Adnan E, Ishizaki J, Miyazaki T, Tanaka Y, Matsumoto T, et al. Novel Autoantigens Associated with Lupus Nephritis. PLoS ONE. 2015;10:e0126564 pubmed publisher
flow cytometry; mouse; fig 2	Durrans A, Gao D, Gupta R, Fischer K, Choi H, El Rayes T, et al. Identification of Reprogrammed Myeloid Cell Transcriptomes in NSCLC. PLoS ONE. 2015;10:e0129123 pubmed publisher
flow cytometry; mouse; fig 7	McCully M, Collins P, Hughes T, Thomas C, Billen J, O Donnell V, et al. Skin Metabolites Define a New Paradigm in the Localization of Skin Tropic Memory T Cells. J Immunol. 2015;195:96-104 pubmed publisher
flow cytometry; mouse	Jacque E, Schweighoffer E, Tybulewicz V, Ley S. BAFF activation of the ERK5 MAP kinase pathway regulates B cell survival. J Exp Med. 2015;212:883-92 pubmed publisher
flow cytometry; mouse	Vuillefroy de Silly R, DucimetiÃ¨re L, Yacoub Maroun C, Dietrich P, Derouazi M, Walker P. Phenotypic switch of CD8(+) T cells reactivated under hypoxia toward IL-10 secreting, poorly proliferative effector cells. Eur J Immunol. 2015;45:2263-75 pubmed publisher
flow cytometry; mouse; loading ...; fig 4	Bouchery T, Kyle R, Camberis M, Shepherd A, Filbey K, Smith A, et al. ILC2s and T cells cooperate to ensure maintenance of M2 macrophages for lung immunity against hookworms. Nat Commun. 2015;6:6970 pubmed publisher
flow cytometry; mouse; fig 2	Sharma S, Chintala N, Vadrevu S, Patel J, Karbowniczek M, Markiewski M. Pulmonary alveolar macrophages contribute to the premetastatic niche by suppressing antitumor T cell responses in the lungs. J Immunol. 2015;194:5529-38 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Iwai H, Funatogawa K, Matsumura K, Kato Miyazawa M, Kirikae F, Kiga K, et al. MicroRNA-155 knockout mice are susceptible to Mycobacterium tuberculosis infection. Tuberculosis (Edinb). 2015;95:246-50 pubmed publisher
flow cytometry; mouse; fig 2a	Hu Lieskovan S, Mok S, Homet Moreno B, Tsoi J, Robert L, Goedert L, et al. Improved antitumor activity of immunotherapy with BRAF and MEK inhibitors in BRAF(V600E) melanoma. Sci Transl Med. 2015;7:279ra41 pubmed publisher
blocking or activating experiments; mouse	Rao E, Zhang Y, Zhu G, Hao J, Persson X, Egilmez N, et al. Deficiency of AMPK in CD8+ T cells suppresses their anti-tumor function by inducing protein phosphatase-mediated cell death. Oncotarget. 2015;6:7944-58 pubmed
flow cytometry; mouse; tbl s3	Pannu J, Belle J, Forster M, Duerr C, Shen S, Kane L, et al. Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation. PLoS ONE. 2015;10:e0117304 pubmed publisher
flow cytometry; mouse; fig 4	HÃ¤usler D, Nessler S, Kruse N, BrÃ¼ck W, Metz I. Natalizumab analogon therapy is effective in a B cell-dependent multiple sclerosis model. Neuropathol Appl Neurobiol. 2015;41:814-31 pubmed publisher
flow cytometry; mouse; fig 5	Evans E, Jonason A, Bussler H, Torno S, Veeraraghavan J, Reilly C, et al. Antibody Blockade of Semaphorin 4D Promotes Immune Infiltration into Tumor and Enhances Response to Other Immunomodulatory Therapies. Cancer Immunol Res. 2015;3:689-701 pubmed publisher
flow cytometry; mouse; loading ...; fig 8f	Minkah N, Macaluso M, Oldenburg D, Paden C, White D, McBride K, et al. Absence of the uracil DNA glycosylase of murine gammaherpesvirus 68 impairs replication and delays the establishment of latency in vivo. J Virol. 2015;89:3366-79 pubmed publisher
flow cytometry; mouse; fig 2	Sullivan B, Teijaro J, de la Torre J, Oldstone M. Early virus-host interactions dictate the course of a persistent infection. PLoS Pathog. 2015;11:e1004588 pubmed publisher
flow cytometry; mouse; fig 5	LUCAS B, White A, Ulvmar M, Nibbs R, Sitnik K, Agace W, et al. CCRL1/ACKR4 is expressed in key thymic microenvironments but is dispensable for T lymphopoiesis at steady state in adult mice. Eur J Immunol. 2015;45:574-83 pubmed publisher
flow cytometry; mouse; fig 3	Nakamura Y, Sato K, Yamamoto H, Matsumura K, Matsumoto I, Nomura T, et al. Dectin-2 deficiency promotes Th2 response and mucin production in the lungs after pulmonary infection with Cryptococcus neoformans. Infect Immun. 2015;83:671-81 pubmed publisher
flow cytometry; mouse	Peters A, Burkett P, Sobel R, Buckley C, Watson S, Bettelli E, et al. Podoplanin negatively regulates CD4+ effector T cell responses. J Clin Invest. 2015;125:129-40 pubmed publisher
flow cytometry; mouse; tbl s1	Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed publisher
flow cytometry; mouse; fig 5	Edwards C, Best S, Gun S, Claser C, James K, de Oca M, et al. Spatiotemporal requirements for IRF7 in mediating type I IFN-dependent susceptibility to blood-stage Plasmodium infection. Eur J Immunol. 2015;45:130-41 pubmed publisher
flow cytometry; mouse	Lee Chang C, Bodogai M, Moritoh K, Olkhanud P, Chan A, Croft M, et al. Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity. Blood. 2014;124:1450-9 pubmed publisher
blocking or activating experiments; mouse; 150 ug/mice	Furugaki K, Cui L, Kunisawa Y, Osada K, Shinkai K, Tanaka M, et al. Intraperitoneal administration of a tumor-associated antigen SART3, CD40L, and GM-CSF gene-loaded polyplex micelle elicits a vaccine effect in mouse tumor models. PLoS ONE. 2014;9:e101854 pubmed publisher
flow cytometry; mouse	Chen Z, Ozbun L, Chong N, Wallecha A, Berzofsky J, Khleif S. Episomal expression of truncated listeriolysin O in LmddA-LLO-E7 vaccine enhances antitumor efficacy by preferentially inducing expansions of CD4+FoxP3- and CD8+ T cells. Cancer Immunol Res. 2014;2:911-22 pubmed publisher
flow cytometry; mouse	Smith N, Wissink E, Wang J, Pinello J, Davenport M, Grimson A, et al. Rapid proliferation and differentiation impairs the development of memory CD8+ T cells in early life. J Immunol. 2014;193:177-84 pubmed publisher
flow cytometry; mouse; fig 2 immunohistochemistry; mouse; fig 2	Russell T, Tscharke D. Strikingly poor CD8+ T-cell immunogenicity of vaccinia virus strain MVA in BALB/c mice. Immunol Cell Biol. 2014;92:466-9 pubmed publisher
flow cytometry; mouse	Misumi I, Whitmire J. B cell depletion curtails CD4+ T cell memory and reduces protection against disseminating virus infection. J Immunol. 2014;192:1597-608 pubmed publisher
flow cytometry; mouse; loading ...; fig s2	Brenndörfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol. 2014;192:1671-80 pubmed publisher
flow cytometry; mouse	Li J, Xiong T, Xiao R, Xiong A, Chen J, Altaf E, et al. Anti-CCL25 antibody prolongs skin allograft survival by blocking CCR9 expression and impairing splenic T-cell function. Arch Immunol Ther Exp (Warsz). 2013;61:237-44 pubmed publisher
	Ye Y, Wang C, Zhang X, Hu Q, Zhang Y, Liu Q, et al. A melanin-mediated cancer immunotherapy patch. Sci Immunol. 2017;2: pubmed publisher
	Hwang S, Jang S, Kim M, Kim L, Kim B, Kim H, et al. YY1 inhibits differentiation and function of regulatory T cells by blocking Foxp3 expression and activity. Nat Commun. 2016;7:10789 pubmed publisher
	Kim K, Kim N, Lee G. Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region. PLoS ONE. 2016;11:e0148576 pubmed publisher
	Ren Y, Wang N, Hu W, Zhang X, Xu J, Wan Y. Successive site translocating inoculation potentiates DNA/recombinant vaccinia vaccination. Sci Rep. 2015;5:18099 pubmed publisher
	Cui L, Osada K, Imaizumi A, Kataoka K, Nakano K. Feasibility of a subcutaneously administered block/homo-mixed polyplex micelle as a carrier for DNA vaccination in a mouse tumor model. J Control Release. 2015;206:220-31 pubmed publisher
	Schwartz M, Kolhatkar N, Thouvenel C, Khim S, Rawlings D. CD4+ T cells and CD40 participate in selection and homeostasis of peripheral B cells. J Immunol. 2014;193:3492-502 pubmed publisher
	Lewis M, Pfeil J, Heiss K, Mueller A. CD8(+) T cells mediate robust stage-specific immunity to P. berghei under chemoprophylaxis and this protective environment is not downregulated by the presence of blood-stage infection. PLoS ONE. 2014;9:e88117 pubmed publisher
	del Rio M, Cote Sierra J, Rodriguez Barbosa J. Flt3L-mobilized dendritic cells bearing H2-Kbm1 apoptotic cells do not induce cross-tolerance to CD8+ T cells across a class I MHC mismatched barrier. Transpl Int. 2011;24:501-13 pubmed publisher
	Apte S, Groves P, Olver S, Baz A, Doolan D, Kelso A, et al. IFN-gamma inhibits IL-4-induced type 2 cytokine expression by CD8 T cells in vivo and modulates the anti-tumor response. J Immunol. 2010;185:998-1004 pubmed publisher
	Bankoti J, Burnett A, Navarro S, Miller A, Rase B, Shepherd D. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010;115:422-34 pubmed publisher
	Medyouf H, Gao X, Armstrong F, Gusscott S, Liu Q, Gedman A, et al. Acute T-cell leukemias remain dependent on Notch signaling despite PTEN and INK4A/ARF loss. Blood. 2010;115:1175-84 pubmed publisher
	Jones T, Hallgren J, Humbles A, Burwell T, Finkelman F, Alcaide P, et al. Antigen-induced increases in pulmonary mast cell progenitor numbers depend on IL-9 and CD1d-restricted NKT cells. J Immunol. 2009;183:5251-60 pubmed publisher
	Todd D, McHeyzer Williams L, Kowal C, Lee A, Volpe B, Diamond B, et al. XBP1 governs late events in plasma cell differentiation and is not required for antigen-specific memory B cell development. J Exp Med. 2009;206:2151-9 pubmed publisher
	Riedl P, Wieland A, Lamberth K, Buus S, Lemonnier F, Reifenberg K, et al. Elimination of immunodominant epitopes from multispecific DNA-based vaccines allows induction of CD8 T cells that have a striking antiviral potential. J Immunol. 2009;183:370-80 pubmed publisher
	Lee C, Chiang Y, Chang S, Chong C, Cheng B, Roffler S. Tumor-localized ligation of CD3 and CD28 with systemic regulatory T-cell depletion induces potent innate and adaptive antitumor responses. Clin Cancer Res. 2009;15:2756-66 pubmed publisher
	Britschgi M, Link A, Lissandrin T, Luther S. Dynamic modulation of CCR7 expression and function on naive T lymphocytes in vivo. J Immunol. 2008;181:7681-8 pubmed
	Kingeter L, Schaefer B. Loss of protein kinase C theta, Bcl10, or Malt1 selectively impairs proliferation and NF-kappa B activation in the CD4+ T cell subset. J Immunol. 2008;181:6244-54 pubmed
	Geiben Lynn R, Greenland J, Frimpong Boateng K, Van Rooijen N, Hovav A, Letvin N. CD4+ T lymphocytes mediate in vivo clearance of plasmid DNA vaccine antigen expression and potentiate CD8+ T-cell immune responses. Blood. 2008;112:4585-90 pubmed publisher
	Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed publisher
	Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
	Andrews D, Andoniou C, Fleming P, Smyth M, Degli Esposti M. The early kinetics of cytomegalovirus-specific CD8+ T-cell responses are not affected by antigen load or the absence of perforin or gamma interferon. J Virol. 2008;82:4931-7 pubmed publisher
	Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
	Rasmussen J, Cello J, Gil H, Forestal C, Furie M, Thanassi D, et al. Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis. Infect Immun. 2006;74:6590-8 pubmed
	Bouwer H, Alberti Segui C, Montfort M, Berkowitz N, Higgins D. Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen. Proc Natl Acad Sci U S A. 2006;103:5102-7 pubmed
	Kao C, Daniels M, Jameson S. Loss of CD8 and TCR binding to Class I MHC ligands following T cell activation. Int Immunol. 2005;17:1607-17 pubmed
	Ko S, Ko H, Chang W, Park S, Kweon M, Kang C. alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005;175:3309-17 pubmed

product information

Antigen :

CD8a

Apps. Abbrev. :

Flow Cytometry, CyTOF®, IHC, IP, Depletion, Block

Cat # :

100715

Clone :

53-6.7

Item :

LEAF™ Purified anti-mouse CD8a

Isotype :

Rat IgG2a, κ

Other Names :

T8, Lyt2, Ly-2

Size :

50 μg

Price (USD) :

30 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

Mouse thymus or spleen

Application Notes :

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes 3 . The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a + cells. Additional reported applications (for the relevant formats) include: immunoprecipitation 1,3 , in vivo and in vitro cell depletion 2,10,15 , inhibition of CD8 T cell proliferation 3 , blocking of cytotoxicity 3,4 , and immunohistochemical staining 5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100716). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100746) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).