Brilliant Violet 421™ anti-mouse CD4 | BioLegend 100563 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-mouse CD4

catalog :

100563

quantity :

50 μg

price :

230 USD

clonality :

monoclonal

host :

rat

conjugate :

BV421

clone name :

RM4-5

reactivity :

mouse

application :

flow cytometry, immunohistochemistry - frozen section

more info or order :

BioLegend product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig 5a	Azpilikueta A, Agorreta J, Labiano S, PÃ©rez Gracia J, SÃ¡nchez Paulete A, Aznar M, et al. Successful Immunotherapy against a Transplantable Mouse Squamous Lung Carcinoma with Anti-PD-1 and Anti-CD137 Monoclonal Antibodies. J Thorac Oncol. 2016;11:524-36 pubmed publisher
	Chen E, Tay N, Brzostek J, Gascoigne N, Rybakin V. A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation. Front Immunol. 2019;10:1718 pubmed publisher
	Baptista A, Gola A, Huang Y, Milanez Almeida P, Torabi Parizi P, Urban J, et al. The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4+ T Cell Peripheralization to Promote Effective Adaptive Immunity. Immunity. 2019;50:1188-1201.e6 pubmed publisher
	LaFleur M, Nguyen T, Coxe M, Yates K, Trombley J, Weiss S, et al. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nat Commun. 2019;10:1668 pubmed publisher
	Lino A, Dang V, Lampropoulou V, Welle A, Joedicke J, Pohar J, et al. LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma Cells. Immunity. 2018;49:120-133.e9 pubmed publisher
	Chen E, Brzostek J, Gascoigne N, Rybakin V. Development of a screening strategy for new modulators of T cell receptor signaling and T cell activation. Sci Rep. 2018;8:10046 pubmed publisher
	Wang D, Diao H, Getzler A, Rogal W, Frederick M, Milner J, et al. The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation. Immunity. 2018;48:659-674.e6 pubmed publisher
	Stein R, Tanoue T, Szabady R, Bhattarai S, Olle B, Norman J, et al. Computer-guided design of optimal microbial consortia for immune system modulation. elife. 2018;7: pubmed publisher
	Stone S, Wu S, Jamison S, Durose W, Pallais J, Lin W. Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases. Glia. 2018;66:1331-1345 pubmed publisher
	Mamedov M, Scholzen A, Nair R, Cumnock K, Kenkel J, Oliveira J, et al. A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence. Immunity. 2018;48:350-363.e7 pubmed publisher
	Bradley C, Teng F, Felix K, Sano T, Naskar D, Block K, et al. Segmented Filamentous Bacteria Provoke Lung Autoimmunity by Inducing Gut-Lung Axis Th17 Cells Expressing Dual TCRs. Cell Host Microbe. 2017;22:697-704.e4 pubmed publisher
	Gimblet C, Meisel J, Loesche M, Cole S, Horwinski J, Novais F, et al. Cutaneous Leishmaniasis Induces a Transmissible Dysbiotic Skin Microbiota that Promotes Skin Inflammation. Cell Host Microbe. 2017;22:13-24.e4 pubmed publisher
	Rodriguez Ruiz M, Rodríguez I, Garasa S, Barbés B, Solorzano J, Perez Gracia J, et al. Abscopal Effects of Radiotherapy Are Enhanced by Combined Immunostimulatory mAbs and Are Dependent on CD8 T Cells and Crosspriming. Cancer Res. 2016;76:5994-6005 pubmed
	Mairhofer D, Ortner D, Tripp C, Schaffenrath S, Fleming V, Heger L, et al. Impaired gp100-Specific CD8(+) T-Cell Responses in the Presence of Myeloid-Derived Suppressor Cells in a Spontaneous Mouse Melanoma Model. J Invest Dermatol. 2015;135:2785-2793 pubmed publisher
	Rodriguez Manzanet R, Sanjuan M, Wu H, Quintana F, Xiao S, Anderson A, et al. T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice. Proc Natl Acad Sci U S A. 2010;107:8706-11 pubmed publisher
	Zaborsky N, Brunner M, Wallner M, Himly M, Karl T, Schwarzenbacher R, et al. Antigen aggregation decides the fate of the allergic immune response. J Immunol. 2010;184:725-35 pubmed publisher
	Shigeta A, Matsumoto M, Tedder T, Lowe J, Miyasaka M, Hirata T. An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation. Blood. 2008;112:4915-23 pubmed publisher
	Matsumoto M, Shigeta A, Furukawa Y, Tanaka T, Miyasaka M, Hirata T. CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin. J Immunol. 2007;178:2499-506 pubmed

product information

Antigen :

CD4

Apps. Abbrev. :

Flow Cytometry, Immunofluorescence Microscopy, IHC-F

Cat # :

100563

Clone :

RM4-5

Item :

Brilliant Violet 421™ anti-mouse CD4

Isotype :

Rat IgG2a, κ

Other Names :

L3T4, T4

Size :

50 μg

Price (USD) :

230 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BV421

Immunogen :

BALB/c mouse thymocytes

Application Notes :

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4 + T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4 + cells 1 , and immunohistochemistry of acetone-fixed frozen tissue sections 2,3,11 and paraffin-embedded sections 11 . Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100575 and 100576 ).

more info or order :

BioLegend product webpage