product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 510™ anti-mouse CD4
catalog :
100559
quantity :
50 μg
price :
230 USD
clonality :
monoclonal
host :
rat
conjugate :
BV510
clone name :
RM4-5
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 23
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5f
Lee J, Hall J, Kroehling L, Wu L, Najar T, Nguyen H, et al. Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease. Cell. 2020;180:79-91.e16 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2c
Liu Z, Gu Y, Chakarov S, Blériot C, Kwok I, Chen X, et al. Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells. Cell. 2019;178:1509-1525.e19 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Cowan J, Baik S, McCarthy N, Parnell S, White A, Jenkinson W, et al. Aire controls the recirculation of murine Foxp3+ regulatory T-cells back to the thymus. Eur J Immunol. 2018;48:844-854 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6a
van der Weyden L, Arends M, Campbell A, Bald T, Wardle Jones H, Griggs N, et al. Genome-wide in vivo screen identifies novel host regulators of metastatic colonization. Nature. 2017;541:233-236 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Filskov J, Andersen P, Agger E, Bukh J. HCV p7 as a novel vaccine-target inducing multifunctional CD4+ and CD8+ T-cells targeting liver cells expressing the viral antigen. Sci Rep. 2019;9:14085 pubmed publisher
Lancaster J, Thyagarajan H, Srinivasan J, Li Y, Hu Z, Ehrlich L. Live-cell imaging reveals the relative contributions of antigen-presenting cell subsets to thymic central tolerance. Nat Commun. 2019;10:2220 pubmed publisher
Miragaia R, Gomes T, Chomka A, Jardine L, Riedel A, Hegazy A, et al. Single-Cell Transcriptomics of Regulatory T Cells Reveals Trajectories of Tissue Adaptation. Immunity. 2019;50:493-504.e7 pubmed publisher
Grzelak A, Polakova I, Smahelova J, Vackova J, Pekarčíková L, Tachezy R, et al. Experimental Combined Immunotherapy of Tumours with Major Histocompatibility Complex Class I Downregulation. Int J Mol Sci. 2018;19: pubmed publisher
Seo G, Shui J, Takahashi D, Song C, Wang Q, Kim K, et al. LIGHT-HVEM Signaling in Innate Lymphoid Cell Subsets Protects Against Enteric Bacterial Infection. Cell Host Microbe. 2018;24:249-260.e4 pubmed publisher
Wang D, Diao H, Getzler A, Rogal W, Frederick M, Milner J, et al. The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation. Immunity. 2018;48:659-674.e6 pubmed publisher
Echevarría Vargas I, Reyes Uribe P, Guterres A, Yin X, Kossenkov A, Liu Q, et al. Co-targeting BET and MEK as salvage therapy for MAPK and checkpoint inhibitor-resistant melanoma. EMBO Mol Med. 2018;10: pubmed publisher
Revathikumar P, Estelius J, Karmakar U, Le Maitre E, Korotkova M, Jakobsson P, et al. Microsomal prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry of the cholinergic anti-inflammatory pathway in endotoxaemic mouse spleen. PLoS ONE. 2018;13:e0193210 pubmed publisher
Blanchfield L, Sabatino J, Lawrence L, Evavold B. NFM Cross-Reactivity to MOG Does Not Expand a Critical Threshold Level of High-Affinity T Cells Necessary for Onset of Demyelinating Disease. J Immunol. 2017;199:2680-2691 pubmed publisher
Galle Treger L, Suzuki Y, Patel N, Sankaranarayanan I, Aron J, Maazi H, et al. Nicotinic acetylcholine receptor agonist attenuates ILC2-dependent airway hyperreactivity. Nat Commun. 2016;7:13202 pubmed publisher
Teixeira L, Marques R, Ferreirinha P, Bezerra F, Melo J, Moreira J, et al. Enrichment of IFN-? producing cells in different murine adipose tissue depots upon infection with an apicomplexan parasite. Sci Rep. 2016;6:23475 pubmed publisher
Lofano G, Mancini F, Salvatore G, Cantisani R, Monaci E, Carrisi C, et al. Oil-in-Water Emulsion MF59 Increases Germinal Center B Cell Differentiation and Persistence in Response to Vaccination. J Immunol. 2015;195:1617-27 pubmed publisher
Neubert K, Lehmann C, Heger L, Baranska A, Staedtler A, Buchholz V, et al. Antigen delivery to CD11c+CD8- dendritic cells induces protective immune responses against experimental melanoma in mice in vivo. J Immunol. 2014;192:5830-8 pubmed publisher
Blankenhaus B, Reitz M, Brenz Y, Eschbach M, Hartmann W, Haben I, et al. Foxp3? regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice. PLoS Pathog. 2014;10:e1003913 pubmed publisher
Rodriguez Manzanet R, Sanjuan M, Wu H, Quintana F, Xiao S, Anderson A, et al. T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice. Proc Natl Acad Sci U S A. 2010;107:8706-11 pubmed publisher
Zaborsky N, Brunner M, Wallner M, Himly M, Karl T, Schwarzenbacher R, et al. Antigen aggregation decides the fate of the allergic immune response. J Immunol. 2010;184:725-35 pubmed publisher
Shigeta A, Matsumoto M, Tedder T, Lowe J, Miyasaka M, Hirata T. An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation. Blood. 2008;112:4915-23 pubmed publisher
Matsumoto M, Shigeta A, Furukawa Y, Tanaka T, Miyasaka M, Hirata T. CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin. J Immunol. 2007;178:2499-506 pubmed
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
100559
Clone :
RM4-5
Item :
Brilliant Violet 510™ anti-mouse CD4
Isotype :
Rat IgG2a, κ
Other Names :
L3T4, T4
Size :
50 μg
Price (USD) :
230 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV510
Immunogen :
BALB/c mouse thymocytes
Application Notes :
The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4 + T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4 + cells 1 , and immunohistochemistry of acetone-fixed frozen tissue sections 2,3,11 and paraffin-embedded sections 11 . Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100575 and 100576 ).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA