product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 510™ anti-mouse CD4
catalog :
100553
quantity :
125 μL
price :
155 USD
clonality :
monoclonal
host :
rat
conjugate :
BV510
clone name :
RM4-5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:150; loading ...; fig s5a
Bliss C, Parsons A, Nachbagauer R, Hamilton J, Cappuccini F, Ulaszewska M, et al. Targeting Antigen to the Surface of EVs Improves the In Vivo Immunogenicity of Human and Non-human Adenoviral Vaccines in Mice. Mol Ther Methods Clin Dev. 2020;16:108-125 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Cowan J, Baik S, McCarthy N, Parnell S, White A, Jenkinson W, et al. Aire controls the recirculation of murine Foxp3+ regulatory T-cells back to the thymus. Eur J Immunol. 2018;48:844-854 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6a
van der Weyden L, Arends M, Campbell A, Bald T, Wardle Jones H, Griggs N, et al. Genome-wide in vivo screen identifies novel host regulators of metastatic colonization. Nature. 2017;541:233-236 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Filskov J, Andersen P, Agger E, Bukh J. HCV p7 as a novel vaccine-target inducing multifunctional CD4+ and CD8+ T-cells targeting liver cells expressing the viral antigen. Sci Rep. 2019;9:14085 pubmed publisher
Lancaster J, Thyagarajan H, Srinivasan J, Li Y, Hu Z, Ehrlich L. Live-cell imaging reveals the relative contributions of antigen-presenting cell subsets to thymic central tolerance. Nat Commun. 2019;10:2220 pubmed publisher
Miragaia R, Gomes T, Chomka A, Jardine L, Riedel A, Hegazy A, et al. Single-Cell Transcriptomics of Regulatory T Cells Reveals Trajectories of Tissue Adaptation. Immunity. 2019;50:493-504.e7 pubmed publisher
Grzelak A, Polakova I, Smahelova J, Vackova J, Pekarčíková L, Tachezy R, et al. Experimental Combined Immunotherapy of Tumours with Major Histocompatibility Complex Class I Downregulation. Int J Mol Sci. 2018;19: pubmed publisher
Seo G, Shui J, Takahashi D, Song C, Wang Q, Kim K, et al. LIGHT-HVEM Signaling in Innate Lymphoid Cell Subsets Protects Against Enteric Bacterial Infection. Cell Host Microbe. 2018;24:249-260.e4 pubmed publisher
Wang D, Diao H, Getzler A, Rogal W, Frederick M, Milner J, et al. The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation. Immunity. 2018;48:659-674.e6 pubmed publisher
Echevarría Vargas I, Reyes Uribe P, Guterres A, Yin X, Kossenkov A, Liu Q, et al. Co-targeting BET and MEK as salvage therapy for MAPK and checkpoint inhibitor-resistant melanoma. EMBO Mol Med. 2018;10: pubmed publisher
Revathikumar P, Estelius J, Karmakar U, Le Maitre E, Korotkova M, Jakobsson P, et al. Microsomal prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry of the cholinergic anti-inflammatory pathway in endotoxaemic mouse spleen. PLoS ONE. 2018;13:e0193210 pubmed publisher
Blanchfield L, Sabatino J, Lawrence L, Evavold B. NFM Cross-Reactivity to MOG Does Not Expand a Critical Threshold Level of High-Affinity T Cells Necessary for Onset of Demyelinating Disease. J Immunol. 2017;199:2680-2691 pubmed publisher
Galle Treger L, Suzuki Y, Patel N, Sankaranarayanan I, Aron J, Maazi H, et al. Nicotinic acetylcholine receptor agonist attenuates ILC2-dependent airway hyperreactivity. Nat Commun. 2016;7:13202 pubmed publisher
Teixeira L, Marques R, Ferreirinha P, Bezerra F, Melo J, Moreira J, et al. Enrichment of IFN-? producing cells in different murine adipose tissue depots upon infection with an apicomplexan parasite. Sci Rep. 2016;6:23475 pubmed publisher
Lofano G, Mancini F, Salvatore G, Cantisani R, Monaci E, Carrisi C, et al. Oil-in-Water Emulsion MF59 Increases Germinal Center B Cell Differentiation and Persistence in Response to Vaccination. J Immunol. 2015;195:1617-27 pubmed publisher
Neubert K, Lehmann C, Heger L, Baranska A, Staedtler A, Buchholz V, et al. Antigen delivery to CD11c+CD8- dendritic cells induces protective immune responses against experimental melanoma in mice in vivo. J Immunol. 2014;192:5830-8 pubmed publisher
Blankenhaus B, Reitz M, Brenz Y, Eschbach M, Hartmann W, Haben I, et al. Foxp3? regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice. PLoS Pathog. 2014;10:e1003913 pubmed publisher
Rodriguez Manzanet R, Sanjuan M, Wu H, Quintana F, Xiao S, Anderson A, et al. T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice. Proc Natl Acad Sci U S A. 2010;107:8706-11 pubmed publisher
Zaborsky N, Brunner M, Wallner M, Himly M, Karl T, Schwarzenbacher R, et al. Antigen aggregation decides the fate of the allergic immune response. J Immunol. 2010;184:725-35 pubmed publisher
Shigeta A, Matsumoto M, Tedder T, Lowe J, Miyasaka M, Hirata T. An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation. Blood. 2008;112:4915-23 pubmed publisher
Matsumoto M, Shigeta A, Furukawa Y, Tanaka T, Miyasaka M, Hirata T. CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin. J Immunol. 2007;178:2499-506 pubmed
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
100553
Clone :
RM4-5
Item :
Brilliant Violet 510™ anti-mouse CD4
Isotype :
Rat IgG2a, κ
Other Names :
L3T4, T4
Size :
125 μL
Price (USD) :
155 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV510
Immunogen :
BALB/c mouse thymocytes
Application Notes :
The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4 + T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4 + cells 1 , and immunohistochemistry of acetone-fixed frozen tissue sections 2,3,11 and paraffin-embedded sections 11 . Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100575 and 100576 ).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA