product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 650™ anti-mouse CD4
catalog :
100546
quantity :
500 μL
price :
435 USD
clonality :
monoclonal
host :
rat
conjugate :
BV650
clone name :
RM4-5
reactivity :
human, mouse
application :
flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 36
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig 1e
Bartsch P, Kilian C, Hellmig M, Paust H, Borchers A, Sivayoganathan A, et al. Th17 cell plasticity towards a T-bet-dependent Th1 phenotype is required for bacterial control in Staphylococcus aureus infection. PLoS Pathog. 2022;18:e1010430 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 6i
  • flow cytometry; mouse; loading ...; fig 2a
Quách T, Huang W, Sahu R, Diadhiou C, Raparia C, Johnson R, et al. Context-dependent induction of autoimmunity by TNF signaling deficiency. JCI Insight. 2022;7: pubmed publisher
  • flow cytometry; mouse; loading ...
Hering L, Katkeviciute E, Schwarzfischer M, Niechcial A, Riggs J, Wawrzyniak M, et al. Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N2 in Dendritic Cells to Protect from Elevated Colitis. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1s3, 4c
Manils J, Webb L, Howes A, Janzen J, Boeing S, Bowcock A, et al. CARD14E138A signalling in keratinocytes induces TNF-dependent skin and systemic inflammation. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s4, s6
Aslan K, Turco V, Blobner J, Sonner J, Liuzzi A, Núñez N, et al. Heterogeneity of response to immune checkpoint blockade in hypermutated experimental gliomas. Nat Commun. 2020;11:931 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Du X, de Almeida P, Manieri N, de Almeida Nagata D, Wu T, Harden Bowles K, et al. CD226 regulates natural killer cell antitumor responses via phosphorylation-mediated inactivation of transcription factor FOXO1. Proc Natl Acad Sci U S A. 2018;115:E11731-E11740 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Ibitokou S, Dillon B, Sinha M, Szczesny B, Delgadillo A, Reda Abdelrahman D, et al. Early Inhibition of Fatty Acid Synthesis Reduces Generation of Memory Precursor Effector T Cells in Chronic Infection. J Immunol. 2018;200:643-656 pubmed publisher
  • flow cytometry; mouse; 1:200; tbl 1
Jackson Jones L, Duncan S, Magalhaes M, Campbell S, Maizels R, McSorley H, et al. Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. Nat Commun. 2016;7:12651 pubmed publisher
Wang G, Sweren E, Liu H, Wier E, Alphonse M, Chen R, et al. Bacteria induce skin regeneration via IL-1β signaling. Cell Host Microbe. 2021;29:777-791.e6 pubmed publisher
Sanmarco L, Wheeler M, Gutiérrez Vázquez C, Polonio C, Linnerbauer M, Pinho Ribeiro F, et al. Gut-licensed IFNγ+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes. Nature. 2021;: pubmed publisher
Hering L, Katkeviciute E, Schwarzfischer M, Busenhart P, Gottier C, Mrdjen D, et al. Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance. Front Immunol. 2020;11:1856 pubmed publisher
Asadi Shahmirzadi A, Edgar D, Liao C, Hsu Y, Lucanic M, Asadi Shahmirzadi A, et al. Alpha-Ketoglutarate, an Endogenous Metabolite, Extends Lifespan and Compresses Morbidity in Aging Mice. Cell Metab. 2020;32:447-456.e6 pubmed publisher
Shook B, Wasko R, Mano O, Rutenberg Schoenberg M, Rudolph M, Zirak B, et al. Dermal Adipocyte Lipolysis and Myofibroblast Conversion Are Required for Efficient Skin Repair. Cell Stem Cell. 2020;26:880-895.e6 pubmed publisher
Flamar A, Klose C, Moeller J, Mahlakõiv T, Bessman N, Zhang W, et al. Interleukin-33 Induces the Enzyme Tryptophan Hydroxylase 1 to Promote Inflammatory Group 2 Innate Lymphoid Cell-Mediated Immunity. Immunity. 2020;52:606-619.e6 pubmed publisher
Horková V, Drobek A, Mueller D, Gubser C, Niederlova V, Wyss L, et al. Dynamics of the Coreceptor-LCK Interactions during T Cell Development Shape the Self-Reactivity of Peripheral CD4 and CD8 T Cells. Cell Rep. 2020;30:1504-1514.e7 pubmed publisher
Chen L, Christian D, Kochanowsky J, Phan A, Clark J, Wang S, et al. The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses. J Exp Med. 2020;217: pubmed publisher
Blagih J, Zani F, Chakravarty P, Hennequart M, Pilley S, Hobor S, et al. Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses. Cell Rep. 2020;30:481-496.e6 pubmed publisher
Li J, Yuan S, Norgard R, Yan F, Yamazoe T, Blanco A, et al. Tumor Cell-Intrinsic USP22 Suppresses Antitumor Immunity in Pancreatic Cancer. Cancer Immunol Res. 2020;8:282-291 pubmed publisher
Chen Y, Chen R, Ahmad S, Verma R, Kasturi S, Amaya L, et al. N6-Methyladenosine Modification Controls Circular RNA Immunity. Mol Cell. 2019;: pubmed publisher
Liu C, Chikina M, Deshpande R, Menk A, Wang T, Tabib T, et al. Treg Cells Promote the SREBP1-Dependent Metabolic Fitness of Tumor-Promoting Macrophages via Repression of CD8+ T Cell-Derived Interferon-γ. Immunity. 2019;51:381-397.e6 pubmed publisher
Casulli J, Fife M, Houston S, Rossi S, Dow J, Williamson E, et al. CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality. Nat Commun. 2019;10:2121 pubmed publisher
Schmidleithner L, Thabet Y, Schönfeld E, Köhne M, Sommer D, Abdullah Z, et al. Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Immunity. 2019;50:1232-1248.e14 pubmed publisher
Mathur A, Zirak B, Boothby I, Tan M, Cohen J, Mauro T, et al. Treg-Cell Control of a CXCL5-IL-17 Inflammatory Axis Promotes Hair-Follicle-Stem-Cell Differentiation During Skin-Barrier Repair. Immunity. 2019;50:655-667.e4 pubmed publisher
Webb L, Barbarulo A, Huysentruyt J, Vanden Berghe T, Takahashi N, Ley S, et al. Survival of Single Positive Thymocytes Depends upon Developmental Control of RIPK1 Kinase Signaling by the IKK Complex Independent of NF-κB. Immunity. 2019;50:348-361.e4 pubmed publisher
Barry K, Hsu J, Broz M, Cueto F, Binnewies M, Combes A, et al. A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments. Nat Med. 2018;24:1178-1191 pubmed publisher
Waight J, Chand D, Dietrich S, Gombos R, Horn T, Gonzalez A, et al. Selective FcγR Co-engagement on APCs Modulates the Activity of Therapeutic Antibodies Targeting T Cell Antigens. Cancer Cell. 2018;33:1033-1047.e5 pubmed publisher
Macal M, Jo Y, Dallari S, Chang A, Dai J, Swaminathan S, et al. Self-Renewal and Toll-like Receptor Signaling Sustain Exhausted Plasmacytoid Dendritic Cells during Chronic Viral Infection. Immunity. 2018;48:730-744.e5 pubmed publisher
Mrdjen D, Pavlovic A, Hartmann F, Schreiner B, Utz S, Leung B, et al. High-Dimensional Single-Cell Mapping of Central Nervous System Immune Cells Reveals Distinct Myeloid Subsets in Health, Aging, and Disease. Immunity. 2018;48:380-395.e6 pubmed publisher
Hossain D, Javaid S, Cai M, Zhang C, Sawant A, Hinton M, et al. Dinaciclib induces immunogenic cell death and enhances anti-PD1-mediated tumor suppression. J Clin Invest. 2018;128:644-654 pubmed publisher
Scharschmidt T, Vasquez K, Pauli M, Leitner E, Chu K, Truong H, et al. Commensal Microbes and Hair Follicle Morphogenesis Coordinately Drive Treg Migration into Neonatal Skin. Cell Host Microbe. 2017;21:467-477.e5 pubmed publisher
Valladao A, Frevert C, Koch L, Campbell D, Ziegler S. STAT6 Regulates the Development of Eosinophilic versus Neutrophilic Asthma in Response to Alternaria alternata. J Immunol. 2016;197:4541-4551 pubmed
Rodriguez Manzanet R, Sanjuan M, Wu H, Quintana F, Xiao S, Anderson A, et al. T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice. Proc Natl Acad Sci U S A. 2010;107:8706-11 pubmed publisher
Zaborsky N, Brunner M, Wallner M, Himly M, Karl T, Schwarzenbacher R, et al. Antigen aggregation decides the fate of the allergic immune response. J Immunol. 2010;184:725-35 pubmed publisher
Shigeta A, Matsumoto M, Tedder T, Lowe J, Miyasaka M, Hirata T. An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation. Blood. 2008;112:4915-23 pubmed publisher
Matsumoto M, Shigeta A, Furukawa Y, Tanaka T, Miyasaka M, Hirata T. CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin. J Immunol. 2007;178:2499-506 pubmed
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
100546
Clone :
RM4-5
Item :
Brilliant Violet 650™ anti-mouse CD4
Isotype :
Rat IgG2a, κ
Other Names :
L3T4, T4
Size :
500 μL
Price (USD) :
435 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV650
Immunogen :
BALB/c mouse thymocytes
Application Notes :
The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4 + T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4 + cells 1 , and immunohistochemistry of acetone-fixed frozen tissue sections 2,3,11 and paraffin-embedded sections 11 . Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576 ).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA