product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP anti-mouse CD4
catalog :
100538
quantity :
100 μg
price :
237 USD
clonality :
monoclonal
host :
rat
conjugate :
PerCP
clone name :
RM4-5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 55
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 6f
Piao W, Li L, Saxena V, Iyyathurai J, Lakhan R, Zhang Y, et al. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration. Nat Commun. 2022;13:2176 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Purvis H, Clarke F, Jordan C, Blanco C, Cornish G, Dai X, et al. Protein tyrosine phosphatase PTPN22 regulates IL-1β dependent Th17 responses by modulating dectin-1 signaling in mice. Eur J Immunol. 2018;48:306-315 pubmed publisher
  • flow cytometry; mouse; loading ...
Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
  • flow cytometry; mouse; fig 1a
Rudolph H, Klopstein A, Gruber I, Blatti C, Lyck R, Engelhardt B. Postarrest stalling rather than crawling favors CD8(+) over CD4(+) T-cell migration across the blood-brain barrier under flow in vitro. Eur J Immunol. 2016;46:2187-203 pubmed publisher
  • flow cytometry; mouse
Thauland T, Koguchi Y, Dustin M, Parker D. CD28-CD80 interactions control regulatory T cell motility and immunological synapse formation. J Immunol. 2014;193:5894-903 pubmed publisher
Voss L, Howarth A, Wittenborn T, Hummelgaard S, Juul Madsen K, Kastberg K, et al. The extrafollicular response is sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus. Front Immunol. 2022;13:1021370 pubmed publisher
Tao Z, Ding W, Cheng Z, Feng Y, Kang Z, Qiu R, et al. Preclinical Study of Plasmodium Immunotherapy Combined with Radiotherapy for Solid Tumors. Cells. 2022;11: pubmed publisher
Knizkova D, Pribikova M, Draberova H, Semberova T, Trivic T, Synackova A, et al. CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology. Nat Immunol. 2022;23:1644-1652 pubmed publisher
Br xfc ggemann T, Carlo T, Krishnamoorthy N, Duvall M, Abdulnour R, Nijmeh J, et al. Mouse phospholipid phosphatase 6 regulates dendritic cell cholesterol, macropinocytosis, and allergen sensitization. iScience. 2022;25:105185 pubmed publisher
Kremenovic M, Chan A, Feng B, B xe4 riswyl L, Robatel S, Gruber T, et al. BCG hydrogel promotes CTSS-mediated antigen processing and presentation, thereby suppressing metastasis and prolonging survival in melanoma. J Immunother Cancer. 2022;10: pubmed publisher
Friedman D, Kizerwetter M, Belmonte P, Rajcula M, Theodore K, Kim Lee H, et al. Cutting Edge: Enhanced Antitumor Immunity in ST8Sia6 Knockout Mice. J Immunol. 2022;208:1845-1850 pubmed publisher
Cha J, Chan L, Wang Y, Chu Y, Wang C, Lee H, et al. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer. J Biol Chem. 2022;298:101817 pubmed publisher
Tian H, Wang G, Wang Q, Zhang B, Jiang G, Li H, et al. Complement C1q binding protein regulates T cells' mitochondrial fitness to affect their survival, proliferation, and anti-tumor immune function. Cancer Sci. 2022;113:875-890 pubmed publisher
Tang L, Li G, Zheng Y, Hou C, Gao Y, Hao Y, et al. Tim-3 Relieves Experimental Autoimmune Encephalomyelitis by Suppressing MHC-II. Front Immunol. 2021;12:770402 pubmed publisher
Koenig A, Vaeth M, Xiao Y, Chiarolla C, Erapaneedi R, Klein M, et al. NFATc1/αA and Blimp-1 Support the Follicular and Effector Phenotype of Tregs. Front Immunol. 2021;12:791100 pubmed publisher
Sturmlechner I, Zhang C, Sine C, van Deursen E, Jeganathan K, Hamada N, et al. p21 produces a bioactive secretome that places stressed cells under immunosurveillance. Science. 2021;374:eabb3420 pubmed publisher
Fitzgerald B, Connolly K, Cui C, Fagerberg E, Mariuzza D, Hornick N, et al. A mouse model for the study of anti-tumor T cell responses in Kras-driven lung adenocarcinoma. Cell Rep Methods. 2021;1: pubmed publisher
Robles Oteiza C, Ayeni D, Levy S, Homer R, Kaech S, Politi K. Elevated murine HB-EGF confers sensitivity to diphtheria toxin in EGFR-mutant lung adenocarcinoma. Dis Model Mech. 2021;14: pubmed publisher
Hao L, Mao Y, Park J, Bae E, Park B. Repurposing the anthelmintic praziquantel to treat psoriasis. Br J Pharmacol. 2021;178:4726-4740 pubmed publisher
Okuniewska M, Fang V, Baeyens A, Raghavan V, Lee J, Littman D, et al. SPNS2 enables T cell egress from lymph nodes during an immune response. Cell Rep. 2021;36:109368 pubmed publisher
Shrivastava T, Singh B, Rizvi Z, Verma R, Goswami S, Vishwakarma P, et al. Comparative Immunomodulatory Evaluation of the Receptor Binding Domain of the SARS-CoV-2 Spike Protein; a Potential Vaccine Candidate Which Imparts Potent Humoral and Th1 Type Immune Response in a Mouse Model. Front Immunol. 2021;12:641447 pubmed publisher
Derada Troletti C, Enzmann G, Chiurchiù V, Kamermans A, Tietz S, Norris P, et al. Pro-resolving lipid mediator lipoxin A4 attenuates neuro-inflammation by modulating T cell responses and modifies the spinal cord lipidome. Cell Rep. 2021;35:109201 pubmed publisher
Roy S, Rizvi Z, Clarke A, MacDonald F, Pandey A, Zaiss D, et al. EGFR-HIF1α signaling positively regulates the differentiation of IL-9 producing T helper cells. Nat Commun. 2021;12:3182 pubmed publisher
Friedman D, Crotts S, Shapiro M, Rajcula M, McCue S, Liu X, et al. ST8Sia6 Promotes Tumor Growth in Mice by Inhibiting Immune Responses. Cancer Immunol Res. 2021;9:952-966 pubmed publisher
Ito Y, Sasaki T, Li Y, Tanoue T, Sugiura Y, Skelly A, et al. Staphylococcus cohnii is a potentially biotherapeutic skin commensal alleviating skin inflammation. Cell Rep. 2021;35:109052 pubmed publisher
Köchl R, Vanes L, Llorian Sopena M, Chakravarty P, Hartweger H, Fountain K, et al. Critical role of WNK1 in MYC-dependent early mouse thymocyte development. elife. 2020;9: pubmed publisher
Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, et al. CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model. Front Immunol. 2020;11:1883 pubmed publisher
Gruber T, Kremenovic M, Sadozai H, Rombini N, Baeriswyl L, Maibach F, et al. IL-32γ potentiates tumor immunity in melanoma. JCI Insight. 2020;5: pubmed publisher
Yang R, Huang H, Cui S, Zhou Y, Zhang T, Zhou Y. IFN-γ promoted exosomes from mesenchymal stem cells to attenuate colitis via miR-125a and miR-125b. Cell Death Dis. 2020;11:603 pubmed publisher
Roy S, Goel R, Aggarwal S, Asthana S, Yadav A, Awasthi A. Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells. Sci Rep. 2020;10:10992 pubmed publisher
Oguri Y, Shinoda K, Kim H, Alba D, Bolus W, Wang Q, et al. CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling. Cell. 2020;: pubmed publisher
Martens R, Permanyer M, Werth K, Yu K, Braun A, Halle O, et al. Efficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidance. Nat Commun. 2020;11:1114 pubmed publisher
Swadling L, Pallett L, Diniz M, Baker J, Amin O, Stegmann K, et al. Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence. Cell Rep. 2020;30:687-698.e6 pubmed publisher
Schaftenaar F, Amersfoort J, Douna H, Kröner M, Foks A, Bot I, et al. Induction of HLA-A2 restricted CD8 T cell responses against ApoB100 peptides does not affect atherosclerosis in a humanized mouse model. Sci Rep. 2019;9:17391 pubmed publisher
Van Gool F, Nguyen M, Mumbach M, Satpathy A, Rosenthal W, Giacometti S, et al. A Mutation in the Transcription Factor Foxp3 Drives T Helper 2 Effector Function in Regulatory T Cells. Immunity. 2019;50:362-377.e6 pubmed publisher
Uchimura T, Oyama Y, Deng M, Guo H, Wilson J, Rampanelli E, et al. The Innate Immune Sensor NLRC3 Acts as a Rheostat that Fine-Tunes T Cell Responses in Infection and Autoimmunity. Immunity. 2018;49:1049-1061.e6 pubmed publisher
Riopel M, Vassallo M, Ehinger E, Pattison J, Bowden K, Winkels H, et al. CX3CL1-Fc treatment prevents atherosclerosis in Ldlr KO mice. Mol Metab. 2019;20:89-101 pubmed publisher
Xu L, Huang Q, Wang H, Hao Y, Bai Q, Hu J, et al. The Kinase mTORC1 Promotes the Generation and Suppressive Function of Follicular Regulatory T Cells. Immunity. 2017;47:538-551.e5 pubmed publisher
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Clement M, Marsden M, Stacey M, Abdul Karim J, Gimeno Brias S, Costa Bento D, et al. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016;12:e1006050 pubmed publisher
Yuan M, Li D, An M, Li Q, Zhang L, Wang G. Rediscovering peritoneal macrophages in a murine endometriosis model. Hum Reprod. 2017;32:94-102 pubmed
Wang Y, Jiang X, Zhu J, Dan Yue -, Zhang X, Wang X, et al. IL-21/IL-21R signaling suppresses intestinal inflammation induced by DSS through regulation of Th responses in lamina propria in mice. Sci Rep. 2016;6:31881 pubmed publisher
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Jacque E, Schweighoffer E, Visekruna A, Papoutsopoulou S, Janzen J, Zillwood R, et al. IKK-induced NF-κB1 p105 proteolysis is critical for B cell antibody responses to T cell-dependent antigen. J Exp Med. 2014;211:2085-101 pubmed publisher
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product information
Antigen :
CD4
Apps. Abbrev. :
FC
Cat # :
100538
Clone :
RM4-5
Item :
PerCP anti-mouse CD4
Isotype :
Rat IgG2a, κ
Other Names :
L3T4, T4
Size :
100 μg
Price (USD) :
237 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PERCP
Immunogen :
BALB/c mouse thymocytes
Application Notes :
The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4^+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4^+ cells^1, and immunohistochemistry of acetone-fixed frozen tissue sections^2,3,11 and paraffin-embedded sections^11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576;).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA