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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-mouse CD4
catalog :
100528
quantity :
100 μg
price :
198 USD
clonality :
monoclonal
host :
rat
conjugate :
PE/Cy7
clone name :
RM4-5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 59
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:400; loading ...
James O, Vandereyken M, Marchingo J, Singh F, Bray S, Wilson J, et al. IL-15 and PIM kinases direct the metabolic programming of intestinal intraepithelial lymphocytes. Nat Commun. 2021;12:4290 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e8a
Wan X, Zinselmeyer B, Zakharov P, Vomund A, Taniguchi R, Santambrogio L, et al. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Nature. 2018;560:107-111 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Gounder A, Yokoyama C, Jarjour N, Bricker T, Edelson B, Boon A. Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer. PLoS Pathog. 2018;14:e1007001 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5c
Macdougall C, Wood E, Loschko J, Scagliotti V, Cassidy F, Robinson M, et al. Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets. Cell Metab. 2018;27:588-601.e4 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Miyazaki M, Miyazaki K, Chen K, Jin Y, Turner J, Moore A, et al. The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development. Immunity. 2017;46:818-834.e4 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 2
Du C, Duan Y, Wei W, Cai Y, Chai H, Lv J, et al. Kappa opioid receptor activation alleviates experimental autoimmune encephalomyelitis and promotes oligodendrocyte-mediated remyelination. Nat Commun. 2016;7:11120 pubmed publisher
  • flow cytometry; mouse; 1:300; fig 2
Xue J, Sharma V, Hsieh M, Chawla A, Murali R, Pandol S, et al. Alternatively activated macrophages promote pancreatic fibrosis in chronic pancreatitis. Nat Commun. 2015;6:7158 pubmed publisher
  • flow cytometry; mouse
Olguín J, Fernández J, Salinas N, Juárez I, Rodriguez Sosa M, Campuzano J, et al. Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated Th1 immune response. Microbes Infect. 2015;17:586-95 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Iwai H, Funatogawa K, Matsumura K, Kato Miyazawa M, Kirikae F, Kiga K, et al. MicroRNA-155 knockout mice are susceptible to Mycobacterium tuberculosis infection. Tuberculosis (Edinb). 2015;95:246-50 pubmed publisher
Stegelmeier A, Santry L, Guilleman M, Matuszewska K, Minott J, Yates J, et al. AAV-Vectored Expression of the Vascular Normalizing Agents 3TSR and Fc3TSR, and the Anti-Angiogenic Bevacizumab Extends Survival in a Murine Model of End-Stage Epithelial Ovarian Carcinoma. Biomedicines. 2022;10: pubmed publisher
Manivasagam S, Williams J, Vollmer L, Bollman B, Bartleson J, Ai S, et al. Targeting IFN-λ Signaling Promotes Recovery from Central Nervous System Autoimmunity. J Immunol. 2022;208:1341-1351 pubmed publisher
Blake S, James J, Ryan F, Caparros Martin J, Eden G, Tee Y, et al. The immunotoxicity, but not anti-tumor efficacy, of anti-CD40 and anti-CD137 immunotherapies is dependent on the gut microbiota. Cell Rep Med. 2021;2:100464 pubmed publisher
Xing J, Zhou X, Fang M, Zhang E, Minze L, Zhang Z. DHX15 is required to control RNA virus-induced intestinal inflammation. Cell Rep. 2021;35:109205 pubmed publisher
Mandal R, Denny J, Namazzi R, Opoka R, Datta D, John C, et al. Dynamic modulation of spleen germinal center reactions by gut bacteria during Plasmodium infection. Cell Rep. 2021;35:109094 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Kaczanowska S, Beury D, Gopalan V, Tycko A, Qin H, Clements M, et al. Genetically engineered myeloid cells rebalance the core immune suppression program in metastasis. Cell. 2021;184:2033-2052.e21 pubmed publisher
Jassinskaja M, Pimková K, Arh N, Johansson E, Davoudi M, Pereira C, et al. Ontogenic shifts in cellular fate are linked to proteotype changes in lineage-biased hematopoietic progenitor cells. Cell Rep. 2021;34:108894 pubmed publisher
Khouili S, Cook E, Hernández García E, Martínez López M, Conde Garrosa R, Iborra S. SHP-1 Regulates Antigen Cross-Presentation and Is Exploited by Leishmania to Evade Immunity. Cell Rep. 2020;33:108468 pubmed publisher
Si Y, Tian Q, Zhao F, Kelly S, Shores L, Camacho D, et al. Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and TH17 responses. Sci Adv. 2020;6:eaba0995 pubmed publisher
Laubreton D, Drajac C, Eléouët J, Rameix Welti M, Lo Man R, Riffault S, et al. Regulatory B Lymphocytes Colonize the Respiratory Tract of Neonatal Mice and Modulate Immune Responses of Alveolar Macrophages to RSV Infection in IL-10-Dependant Manner. Viruses. 2020;12: pubmed publisher
Kim D, Nguyen Q, Lee J, Lee S, Janocha A, Kim S, et al. Anti-inflammatory Roles of Glucocorticoids Are Mediated by Foxp3+ Regulatory T Cells via a miR-342-Dependent Mechanism. Immunity. 2020;53:581-596.e5 pubmed publisher
Chong W, Mattapallil M, Raychaudhuri K, Bing S, Wu S, Zhong Y, et al. The Cytokine IL-17A Limits Th17 Pathogenicity via a Negative Feedback Loop Driven by Autocrine Induction of IL-24. Immunity. 2020;53:384-397.e5 pubmed publisher
Frohner I, Mudrak I, Schüchner S, Anrather D, Hartl M, Sontag J, et al. PP2AC Phospho-Tyr307 Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2AC Modifications Including Leu309 Methylation. Cell Rep. 2020;30:3171-3182.e6 pubmed publisher
Choi J, Eskandari S, Cai S, Sulkaj I, Assaker J, Allos H, et al. Regulatory CD8 T cells that recognize Qa-1 expressed by CD4 T-helper cells inhibit rejection of heart allografts. Proc Natl Acad Sci U S A. 2020;117:6042-6046 pubmed publisher
Hirata S, Nagatake T, Sawane K, Hosomi K, Honda T, Ono S, et al. Maternal ω3 docosapentaenoic acid inhibits infant allergic dermatitis through TRAIL-expressing plasmacytoid dendritic cells in mice. Allergy. 2020;75:1939-1955 pubmed publisher
Vanderleyden I, Fra Bido S, Innocentin S, Stebegg M, Okkenhaug H, Evans Bailey N, et al. Follicular Regulatory T Cells Can Access the Germinal Center Independently of CXCR5. Cell Rep. 2020;30:611-619.e4 pubmed publisher
Lai N, Musser M, Pinho Ribeiro F, Baral P, Jacobson A, Ma P, et al. Gut-Innervating Nociceptor Neurons Regulate Peyer's Patch Microfold Cells and SFB Levels to Mediate Salmonella Host Defense. Cell. 2020;180:33-49.e22 pubmed publisher
Ly A, Liao Y, Pietrzak H, Ioannidis L, Sidwell T, Gloury R, et al. Transcription Factor T-bet in B Cells Modulates Germinal Center Polarization and Antibody Affinity Maturation in Response to Malaria. Cell Rep. 2019;29:2257-2269.e6 pubmed publisher
Renner K, Bruss C, Schnell A, Koehl G, Becker H, Fante M, et al. Restricting Glycolysis Preserves T Cell Effector Functions and Augments Checkpoint Therapy. Cell Rep. 2019;29:135-150.e9 pubmed publisher
Bhattacharjee S, Mejías Luque R, Loffredo Verde E, Toska A, Flossdorf M, Gerhard M, et al. Concomitant Infection of S. mansoni and H. pylori Promotes Promiscuity of Antigen-Experienced Cells and Primes the Liver for a Lower Fibrotic Response. Cell Rep. 2019;28:231-244.e5 pubmed publisher
Kubli S, Vornholz L, Duncan G, Zhou W, Ramachandran P, Fortin J, et al. Fcmr regulates mononuclear phagocyte control of anti-tumor immunity. Nat Commun. 2019;10:2678 pubmed publisher
Miska J, Lee Chang C, Rashidi A, Muroski M, Chang A, Lopez Rosas A, et al. HIF-1α Is a Metabolic Switch between Glycolytic-Driven Migration and Oxidative Phosphorylation-Driven Immunosuppression of Tregs in Glioblastoma. Cell Rep. 2019;27:226-237.e4 pubmed publisher
Hastings A, Hastings K, Uraki R, Hwang J, Gaitsch H, Dhaliwal K, et al. Loss of the TAM Receptor Axl Ameliorates Severe Zika Virus Pathogenesis and Reduces Apoptosis in Microglia. iScience. 2019;13:339-350 pubmed publisher
Thompson P, Shah A, Ntranos V, Van Gool F, Atkinson M, Bhushan A. Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes. Cell Metab. 2019;29:1045-1060.e10 pubmed publisher
Martínez López M, Iborra S, Conde Garrosa R, Mastrangelo A, Danne C, Mann E, et al. Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity. Immunity. 2019;50:446-461.e9 pubmed publisher
Xu X, Xiao W, Zhang Z, Pan J, Yan Y, Zhu T, et al. Anti-pruritic and anti-inflammatory effects of oxymatrine in a mouse model of allergic contact dermatitis. J Dermatol Sci. 2018;: pubmed publisher
Wang D, Diao H, Getzler A, Rogal W, Frederick M, Milner J, et al. The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation. Immunity. 2018;48:659-674.e6 pubmed publisher
Platteel A, Nieuwenhuizen N, Domaszewska T, Schurer S, Zedler U, Brinkmann V, et al. Efficacy Testing of H56 cDNA Tattoo Immunization against Tuberculosis in a Mouse Model. Front Immunol. 2017;8:1744 pubmed publisher
Hayatsu N, Miyao T, Tachibana M, Murakami R, Kimura A, Kato T, et al. Analyses of a Mutant Foxp3 Allele Reveal BATF as a Critical Transcription Factor in the Differentiation and Accumulation of Tissue Regulatory T Cells. Immunity. 2017;47:268-283.e9 pubmed publisher
Quispe Calla N, Vicetti Miguel R, Mei A, Fan S, Gilmore J, Cherpes T. Dendritic cell function and pathogen-specific T cell immunity are inhibited in mice administered levonorgestrel prior to intranasal Chlamydia trachomatis infection. Sci Rep. 2016;6:37723 pubmed publisher
Matsumura K, Iwai H, Kato Miyazawa M, Kirikae F, Zhao J, Yanagawa T, et al. Peroxiredoxin 1 Contributes to Host Defenses against Mycobacterium tuberculosis. J Immunol. 2016;197:3233-3244 pubmed
Campisi L, Barbet G, Ding Y, Esplugues E, Flavell R, Blander J. Apoptosis in response to microbial infection induces autoreactive TH17 cells. Nat Immunol. 2016;17:1084-92 pubmed publisher
Gengenbacher M, Nieuwenhuizen N, Vogelzang A, Liu H, Kaiser P, Schuerer S, et al. Deletion of nuoG from the Vaccine Candidate Mycobacterium bovis BCG ?ureC::hly Improves Protection against Tuberculosis. MBio. 2016;7: pubmed publisher
Wan X, Thomas J, Unanue E. Class-switched anti-insulin antibodies originate from unconventional antigen presentation in multiple lymphoid sites. J Exp Med. 2016;213:967-78 pubmed publisher
Nocera D, Roselli E, Araya P, Nuñez N, Lienenklaus S, Jablonska J, et al. In Vivo Visualizing the IFN-β Response Required for Tumor Growth Control in a Therapeutic Model of Polyadenylic-Polyuridylic Acid Administration. J Immunol. 2016;196:2860-9 pubmed publisher
Belle J, Petrov J, Langlais D, Robert F, Cencic R, Shen S, et al. Repression of p53-target gene Bbc3/PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance. Cell Death Differ. 2016;23:759-75 pubmed publisher
Ryg Cornejo V, Ioannidis L, Ly A, Chiu C, Tellier J, Hill D, et al. Severe Malaria Infections Impair Germinal Center Responses by Inhibiting T Follicular Helper Cell Differentiation. Cell Rep. 2016;14:68-81 pubmed publisher
Schaller M, Ito T, Allen R, Kroetz D, Kittan N, Ptaschinski C, et al. Epigenetic regulation of IL-12-dependent T cell proliferation. J Leukoc Biol. 2015;98:601-13 pubmed publisher
Everard A, Geurts L, Caesar R, Van Hul M, Matamoros S, Duparc T, et al. Intestinal epithelial MyD88 is a sensor switching host metabolism towards obesity according to nutritional status. Nat Commun. 2014;5:5648 pubmed publisher
Ballet R, Emre Y, Jemelin S, Charmoy M, Tacchini Cottier F, Imhof B. Blocking junctional adhesion molecule C enhances dendritic cell migration and boosts the immune responses against Leishmania major. PLoS Pathog. 2014;10:e1004550 pubmed publisher
Carty S, Koretzky G, Jordan M. Interleukin-4 regulates eomesodermin in CD8+ T cell development and differentiation. PLoS ONE. 2014;9:e106659 pubmed publisher
Macagno M, Bandini S, Stramucci L, Quaglino E, Conti L, Balmas E, et al. Multiple roles of perforin in hampering ERBB-2 (Her-2/neu) carcinogenesis in transgenic male mice. J Immunol. 2014;192:5434-41 pubmed publisher
Blankenhaus B, Reitz M, Brenz Y, Eschbach M, Hartmann W, Haben I, et al. Foxp3? regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice. PLoS Pathog. 2014;10:e1003913 pubmed publisher
Narni Mancinelli E, Chaix J, Fenis A, Kerdiles Y, Yessaad N, Reynders A, et al. Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor. Proc Natl Acad Sci U S A. 2011;108:18324-9 pubmed publisher
Vallenback P, Ghatnekar L, Bengtsson B. Structure of the natural transgene PgiC2 in the common grass Festuca ovina. PLoS ONE. 2010;5:e13529 pubmed publisher
Rodriguez Manzanet R, Sanjuan M, Wu H, Quintana F, Xiao S, Anderson A, et al. T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice. Proc Natl Acad Sci U S A. 2010;107:8706-11 pubmed publisher
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Shigeta A, Matsumoto M, Tedder T, Lowe J, Miyasaka M, Hirata T. An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation. Blood. 2008;112:4915-23 pubmed publisher
Matsumoto M, Shigeta A, Furukawa Y, Tanaka T, Miyasaka M, Hirata T. CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin. J Immunol. 2007;178:2499-506 pubmed
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
100528
Clone :
RM4-5
Item :
PE/Cyanine7 anti-mouse CD4
Isotype :
Rat IgG2a, κ
Other Names :
L3T4, T4
Size :
100 μg
Price (USD) :
198 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE_CYANINE7
Immunogen :
BALB/c mouse thymocytes
Application Notes :
The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4 + T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4 + cells 1 , and immunohistochemistry of acetone-fixed frozen tissue sections 2,3,11 and paraffin-embedded sections 11 . Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576 ).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA