product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-mouse CD4
catalog :
100526
quantity :
100 μg
price :
198 USD
clonality :
monoclonal
host :
rat
conjugate :
APC/Cy7
clone name :
RM4-5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 47
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 6f
Que W, Ma K, Hu X, Guo W, Li X. Combinations of anti-GITR antibody and CD28 superagonist induce permanent allograft acceptance by generating type 1 regulatory T cells. Sci Adv. 2022;8:eabo4413 pubmed publisher
  • flow cytometry; mouse; 1:400; fig s5c, s6b
Taniguchi H, Caeser R, Chavan S, Zhan Y, Chow A, Manoj P, et al. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022;39:110814 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig s6f
Xiong W, Gao X, Zhang T, Jiang B, Hu M, Bu X, et al. USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy. Nat Commun. 2022;13:1700 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e2a, e5h
Strickley J, Messerschmidt J, Awad M, Li T, Hasegawa T, Ha D, et al. Immunity to commensal papillomaviruses protects against skin cancer. Nature. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Ding L, Kim H, Wang Q, Kearns M, Jiang T, Ohlson C, et al. PARP Inhibition Elicits STING-Dependent Antitumor Immunity in Brca1-Deficient Ovarian Cancer. Cell Rep. 2018;25:2972-2980.e5 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Tordesillas L, Lozano Ojalvo D, Dunkin D, Mondoulet L, Agudo J, Merad M, et al. PDL2+ CD11b+ dermal dendritic cells capture topical antigen through hair follicles to prime LAP+ Tregs. Nat Commun. 2018;9:5238 pubmed publisher
  • flow cytometry; mouse; fig 6a
Aryal B, Rotllan N, Araldi E, Ramírez C, He S, Chousterman B, et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. 2016;7:12313 pubmed publisher
Koide S, Sigurdsson V, Radulović V, Saito K, Zheng Z, Lang S, et al. CD244 expression represents functional decline of murine hematopoietic stem cells after in vitro culture. iScience. 2022;25:103603 pubmed publisher
Synn C, Kim S, Lee H, Kim M, Kim J, Lee J, et al. SKI-G-801, an AXL kinase inhibitor, blocks metastasis through inducing anti-tumor immune responses and potentiates anti-PD-1 therapy in mouse cancer models. Clin Transl Immunology. 2022;11:e1364 pubmed publisher
Melo Silva C, Alves Peixoto P, Heath N, Tang L, Montoya B, Knudson C, et al. Resistance to lethal ectromelia virus infection requires Type I interferon receptor in natural killer cells and monocytes but not in adaptive immune or parenchymal cells. PLoS Pathog. 2021;17:e1009593 pubmed publisher
Gonzalez Figueroa P, Roco J, Papa I, Núñez Villacís L, Stanley M, Linterman M, et al. Follicular regulatory T cells produce neuritin to regulate B cells. Cell. 2021;184:1775-1789.e19 pubmed publisher
Westfall S, Caracci F, Zhao D, Wu Q, Frolinger T, Simon J, et al. Microbiota metabolites modulate the T helper 17 to regulatory T cell (Th17/Treg) imbalance promoting resilience to stress-induced anxiety- and depressive-like behaviors. Brain Behav Immun. 2021;91:350-368 pubmed publisher
Ringel A, Drijvers J, Baker G, Catozzi A, García Cañaveras J, Gassaway B, et al. Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity. Cell. 2020;183:1848-1866.e26 pubmed publisher
Ma F, Ding M, Lei Y, Luo L, Jiang S, Feng Y, et al. SKIL facilitates tumorigenesis and immune escape of NSCLC via upregulating TAZ/autophagy axis. Cell Death Dis. 2020;11:1028 pubmed publisher
León Letelier R, Castro Medina D, Badillo Godinez O, Tepale Segura A, Huanosta Murillo E, Aguilar Flores C, et al. Induction of Progenitor Exhausted Tissue-Resident Memory CD8+ T Cells Upon Salmonella Typhi Porins Adjuvant Immunization Correlates With Melanoma Control and Anti-PD-1 Immunotherapy Cooperation. Front Immunol. 2020;11:583382 pubmed publisher
Yun H, Yee M, Lathrop K, Kinchington P, Hendricks R, St Leger A. Production of the Cytokine VEGF-A by CD4+ T and Myeloid Cells Disrupts the Corneal Nerve Landscape and Promotes Herpes Stromal Keratitis. Immunity. 2020;53:1050-1062.e5 pubmed publisher
Acharya N, Madi A, Zhang H, Klapholz M, Escobar G, Dulberg S, et al. Endogenous Glucocorticoid Signaling Regulates CD8+ T Cell Differentiation and Development of Dysfunction in the Tumor Microenvironment. Immunity. 2020;53:658-671.e6 pubmed publisher
Peng L, Wang Y, Fei S, Wei C, Tong F, Wu G, et al. The effect of combining Endostar with radiotherapy on blood vessels, tumor-associated macrophages, and T cells in brain metastases of Lewis lung cancer. Transl Lung Cancer Res. 2020;9:745-760 pubmed publisher
Mender I, Zhang A, Ren Z, Han C, Deng Y, Siteni S, et al. Telomere Stress Potentiates STING-Dependent Anti-tumor Immunity. Cancer Cell. 2020;38:400-411.e6 pubmed publisher
Gladow N, Hollmann C, Ramos G, Frantz S, Kerkau T, Beyersdorf N, et al. Treatment of mice with a ligand binding blocking anti-CD28 monoclonal antibody improves healing after myocardial infarction. PLoS ONE. 2020;15:e0227734 pubmed publisher
Kumar R, Bunn P, Singh S, Ng S, Montes De Oca M, de Labastida Rivera F, et al. Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis. Cell Rep. 2020;30:2512-2525.e9 pubmed publisher
Boehm D, Wolf M, Hall J, Wong T, Sen Kilic E, Basinger H, et al. Intranasal acellular pertussis vaccine provides mucosal immunity and protects mice from Bordetella pertussis. NPJ Vaccines. 2019;4:40 pubmed publisher
Howie D, Ten Bokum A, Cobbold S, Yu Z, Kessler B, Waldmann H. A Novel Role for Triglyceride Metabolism in Foxp3 Expression. Front Immunol. 2019;10:1860 pubmed publisher
Kubota S, Tokunaga K, Umezu T, Yokomizo Nakano T, Sun Y, Oshima M, et al. Lineage-specific RUNX2 super-enhancer activates MYC and promotes the development of blastic plasmacytoid dendritic cell neoplasm. Nat Commun. 2019;10:1653 pubmed publisher
Denny J, Powers J, Castro H, Zhang J, Joshi Barve S, Campagna S, et al. Differential Sensitivity to Plasmodium yoelii Infection in C57BL/6 Mice Impacts Gut-Liver Axis Homeostasis. Sci Rep. 2019;9:3472 pubmed publisher
Weng Q, Hu F, Zhang M, Dong Y, Lv C, Wang Y, et al. A protocol for generating induced T cells by reprogramming B cells in vivo. Cell Regen (Lond). 2018;7:7-15 pubmed publisher
Cuburu N, Kim R, Guittard G, Thompson C, Day P, Hamm D, et al. A Prime-Pull-Amplify Vaccination Strategy To Maximize Induction of Circulating and Genital-Resident Intraepithelial CD8+ Memory T Cells. J Immunol. 2019;202:1250-1264 pubmed publisher
Turner J, Ke F, GRIGOROVA I. B Cell Receptor Crosslinking Augments Germinal Center B Cell Selection when T Cell Help Is Limiting. Cell Rep. 2018;25:1395-1403.e4 pubmed publisher
Biton M, Haber A, Rogel N, Burgin G, Beyaz S, Schnell A, et al. T Helper Cell Cytokines Modulate Intestinal Stem Cell Renewal and Differentiation. Cell. 2018;175:1307-1320.e22 pubmed publisher
Ogawa C, Bankoti R, Nguyen T, Hassanzadeh Kiabi N, Nadeau S, Porritt R, et al. Blimp-1 Functions as a Molecular Switch to Prevent Inflammatory Activity in Foxp3+RORγt+ Regulatory T Cells. Cell Rep. 2018;25:19-28.e5 pubmed publisher
Jarick K, Mokhtari Z, Scheller L, Hartweg J, Thusek S, Le D, et al. Photoconversion of Alloreactive T Cells in Murine Peyer's Patches During Acute Graft-Versus-Host Disease: Tracking the Homing Route of Highly Proliferative Cells In Vivo. Front Immunol. 2018;9:1468 pubmed publisher
Cobbold S, Adams E, Howie D, Waldmann H. CD4+ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development. Front Immunol. 2018;9:1381 pubmed publisher
Herndler Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, et al. KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity. 2018;48:716-729.e8 pubmed publisher
Saigusa R, Asano Y, Taniguchi T, Hirabayashi M, Nakamura K, Miura S, et al. Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells. Arthritis Res Ther. 2018;20:23 pubmed publisher
Ding Z, Habtetsion T, Cao Y, Li T, Liu C, Kuczma M, et al. Adjuvant IL-7 potentiates adoptive T cell therapy by amplifying and sustaining polyfunctional antitumor CD4+ T cells. Sci Rep. 2017;7:12168 pubmed publisher
Bankoti R, Ogawa C, Nguyen T, Emadi L, Couse M, Salehi S, et al. Differential regulation of Effector and Regulatory T cell function by Blimp1. Sci Rep. 2017;7:12078 pubmed publisher
Mencarelli A, Cipriani S, Francisci D, Santucci L, Baldelli F, Distrutti E, et al. Highly specific blockade of CCR5 inhibits leukocyte trafficking and reduces mucosal inflammation in murine colitis. Sci Rep. 2016;6:30802 pubmed publisher
Ulrich V, Rotllan N, Araldi E, Luciano A, Skroblin P, Abonnenc M, et al. Chronic miR-29 antagonism promotes favorable plaque remodeling in atherosclerotic mice. EMBO Mol Med. 2016;8:643-53 pubmed publisher
Lu X, Ding Z, Cao Y, Liu C, Habtetsion T, Yu M, et al. Alkylating agent melphalan augments the efficacy of adoptive immunotherapy using tumor-specific CD4+ T cells. J Immunol. 2015;194:2011-21 pubmed publisher
Wei M, Marino J, Trowell A, Zhang H, Stromp Peraino J, Rajasekera P, et al. Diphtheria toxin-based recombinant murine IL-2 fusion toxin for depleting murine regulatory T cells in vivo. Protein Eng Des Sel. 2014;27:289-95 pubmed publisher
Shiomi A, Usui T, Ishikawa Y, Shimizu M, Murakami K, Mimori T. GM-CSF but not IL-17 is critical for the development of severe interstitial lung disease in SKG mice. J Immunol. 2014;193:849-59 pubmed publisher
Brenndörfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol. 2014;192:1671-80 pubmed publisher
Rodriguez Manzanet R, Sanjuan M, Wu H, Quintana F, Xiao S, Anderson A, et al. T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice. Proc Natl Acad Sci U S A. 2010;107:8706-11 pubmed publisher
Zaborsky N, Brunner M, Wallner M, Himly M, Karl T, Schwarzenbacher R, et al. Antigen aggregation decides the fate of the allergic immune response. J Immunol. 2010;184:725-35 pubmed publisher
Shigeta A, Matsumoto M, Tedder T, Lowe J, Miyasaka M, Hirata T. An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation. Blood. 2008;112:4915-23 pubmed publisher
Matsumoto M, Shigeta A, Furukawa Y, Tanaka T, Miyasaka M, Hirata T. CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin. J Immunol. 2007;178:2499-506 pubmed
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
100526
Clone :
RM4-5
Item :
APC/Cyanine7 anti-mouse CD4
Isotype :
Rat IgG2a, κ
Other Names :
L3T4, T4
Size :
100 μg
Price (USD) :
198 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
BALB/c mouse thymocytes
Application Notes :
The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4 + T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4 + cells 1 , and immunohistochemistry of acetone-fixed frozen tissue sections 2,3,11 and paraffin-embedded sections 11 . Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576 ).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA