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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Biotin anti-mouse CD4
catalog :
100508
quantity :
500 μg
price :
114 USD
clonality :
monoclonal
host :
rat
conjugate :
biotin
clone name :
RM4-5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 36
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1G
Yu V, Yusuf R, Oki T, Wu J, Saez B, Wang X, et al. Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells. Cell. 2016;167:1310-1322.e17 pubmed publisher
Giannou A, Kempski J, Shiri A, L xfc cke J, Zhang T, Zhao L, et al. Tissue resident iNKT17 cells facilitate cancer cell extravasation in liver metastasis via interleukin-22. Immunity. 2023;56:125-142.e12 pubmed publisher
Rao X, Dong H, Zhang W, Sun H, Gu W, Zhang X, et al. MiR-493-5p inhibits Th9 cell differentiation in allergic asthma by targeting FOXO1. Respir Res. 2022;23:286 pubmed publisher
Mari xe9 I, Brambilla L, Levy D. Assessing the Presence of Hematopoietic Stem and Progenitor Cells in Mouse Spleen. Bio Protoc. 2022;12:e4438 pubmed publisher
Yang X, Ge L, Wang J. BECN1 modulates hematopoietic stem cells by targeting Caspase-3-GSDME-mediated pyroptosis. Blood Sci. 2020;2:89-99 pubmed publisher
Stofkova A, Zloh M, Andreanska D, Fiserova I, Kubovčiak J, Hejda J, et al. Depletion of Retinal Dopaminergic Activity in a Mouse Model of Rod Dysfunction Exacerbates Experimental Autoimmune Uveoretinitis: A Role for the Gateway Reflex. Int J Mol Sci. 2021;23: pubmed publisher
Lehrke M, Shapiro M, Rajcula M, Kennedy M, McCue S, Medina K, et al. The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in mice. elife. 2021;10: pubmed publisher
Sturmlechner I, Zhang C, Sine C, van Deursen E, Jeganathan K, Hamada N, et al. p21 produces a bioactive secretome that places stressed cells under immunosurveillance. Science. 2021;374:eabb3420 pubmed publisher
Li Z, Wu J, Zhou J, Yuan B, Chen J, Wu W, et al. A Vimentin-Targeting Oral Compound with Host-Directed Antiviral and Anti-Inflammatory Actions Addresses Multiple Features of COVID-19 and Related Diseases. MBio. 2021;12:e0254221 pubmed publisher
Bouchareychas L, Duong P, Phu T, Alsop E, Meechoovet B, Reiman R, et al. High glucose macrophage exosomes enhance atherosclerosis by driving cellular proliferation & hematopoiesis. iScience. 2021;24:102847 pubmed publisher
Gutiérrez Gutiérrez Ó, Felix D, Salvetti A, Amro E, Thems A, Pietsch S, et al. Regeneration in starved planarians depends on TRiC/CCT subunits modulating the unfolded protein response. EMBO Rep. 2021;22:e52905 pubmed publisher
Yi W, Zhang Y, Liu B, Zhou Y, Liao D, Qiao X, et al. Protein S-nitrosylation regulates proteostasis and viability of hematopoietic stem cell during regeneration. Cell Rep. 2021;34:108922 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Trefzer A, Kadam P, Wang S, Pennavaria S, Lober B, Akçabozan B, et al. Dynamic adoption of anergy by antigen-exhausted CD4+ T cells. Cell Rep. 2021;34:108748 pubmed publisher
Wang F, Trier A, Li F, Kim S, Chen Z, Chai J, et al. A basophil-neuronal axis promotes itch. Cell. 2021;184:422-440.e17 pubmed publisher
Wang L, Yu S, Chan E, Chen K, Liu C, Che D, et al. Notch-Regulated Dendritic Cells Restrain Inflammation-Associated Colorectal Carcinogenesis. Cancer Immunol Res. 2021;9:348-361 pubmed publisher
Rohrbeck L, Adori M, Wang S, He C, Tibbitt C, Chernyshev M, et al. GPR43 regulates marginal zone B-cell responses to foreign and endogenous antigens. Immunol Cell Biol. 2021;99:234-243 pubmed publisher
Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, et al. PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2. J Cell Biol. 2020;219: pubmed publisher
Chauveau A, Pirgova G, Cheng H, De Martin A, Zhou F, Wideman S, et al. Visualization of T Cell Migration in the Spleen Reveals a Network of Perivascular Pathways that Guide Entry into T Zones. Immunity. 2020;52:794-807.e7 pubmed publisher
Preglej T, Hamminger P, Luu M, Bulat T, Andersen L, Göschl L, et al. Histone deacetylases 1 and 2 restrain CD4+ cytotoxic T lymphocyte differentiation. JCI Insight. 2020;5: pubmed publisher
Hartmann W, Brunn M, Stetter N, Gagliani N, Muscate F, Stanelle Bertram S, et al. Helminth Infections Suppress the Efficacy of Vaccination against Seasonal Influenza. Cell Rep. 2019;29:2243-2256.e4 pubmed publisher
Huang B, Chen Z, Geng L, Wang J, Liang H, Cao Y, et al. Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways. Cell. 2019;179:1160-1176.e24 pubmed publisher
Miska J, Lee Chang C, Rashidi A, Muroski M, Chang A, Lopez Rosas A, et al. HIF-1α Is a Metabolic Switch between Glycolytic-Driven Migration and Oxidative Phosphorylation-Driven Immunosuppression of Tregs in Glioblastoma. Cell Rep. 2019;27:226-237.e4 pubmed publisher
Chen Z, Amro E, Becker F, Holzer M, Rasa S, Njeru S, et al. Cohesin-mediated NF-κB signaling limits hematopoietic stem cell self-renewal in aging and inflammation. J Exp Med. 2019;216:152-175 pubmed publisher
Zhu C, Xia L, Li F, Zhou L, Weng Q, Li Z, et al. mTOR complexes differentially orchestrates eosinophil development in allergy. Sci Rep. 2018;8:6883 pubmed publisher
Herndler Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, et al. KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity. 2018;48:716-729.e8 pubmed publisher
Zhang C, Yi W, Li F, Du X, Wang H, Wu P, et al. Eosinophil-derived CCL-6 impairs hematopoietic stem cell homeostasis. Cell Res. 2018;28:323-335 pubmed publisher
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Yu V, Yusuf R, Oki T, Wu J, Saez B, Wang X, et al. Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells. Cell. 2017;168:944-945 pubmed publisher
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Rodriguez Manzanet R, Sanjuan M, Wu H, Quintana F, Xiao S, Anderson A, et al. T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice. Proc Natl Acad Sci U S A. 2010;107:8706-11 pubmed publisher
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Matsumoto M, Shigeta A, Furukawa Y, Tanaka T, Miyasaka M, Hirata T. CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin. J Immunol. 2007;178:2499-506 pubmed
product information
Antigen :
CD4
Apps. Abbrev. :
FC
Cat # :
100508
Clone :
RM4-5
Item :
Biotin anti-mouse CD4
Isotype :
Rat IgG2a, κ
Other Names :
L3T4, T4
Size :
500 μg
Price (USD) :
114 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BIOTIN
Immunogen :
BALB/c mouse thymocytes
Application Notes :
The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4^+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4^+ cells^1, and immunohistochemistry of acetone-fixed frozen tissue sections^2,3,11 and paraffin-embedded sections^11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576;).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA