Spark Blue™ 574 anti-mouse CD4 | BioLegend 100489 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Spark Blue™ 574 anti-mouse CD4

catalog :

100489

quantity :

25 μg

price :

132 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

GK1.5

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:200; loading ...	Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
flow cytometry; mouse; fig 3	Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig s22b	Ho D, Tsui Y, Chan L, Sze K, Zhang X, Cheu J, et al. Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma. Nat Commun. 2021;12:3684 pubmed publisher
flow cytometry; mouse; fig 4b	Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
flow cytometry; mouse; loading ...; fig 1s2a	Lebratti T, Lim Y, Cofie A, Andhey P, Jiang X, Scott J, et al. A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection. elife. 2021;10: pubmed publisher
flow cytometry; mouse; loading ...; fig s2a, 5b, 5f	Reis M, Willis G, Fernandez Gonzalez A, Yeung V, Taglauer E, Magaletta M, et al. Mesenchymal Stromal Cell-Derived Extracellular Vesicles Restore Thymic Architecture and T Cell Function Disrupted by Neonatal Hyperoxia. Front Immunol. 2021;12:640595 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 7b	Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed publisher

product information

Antigen :

CD4

Apps. Abbrev. :

Flow Cytometry

Cat # :

100489

Clone :

GK1.5

Item :

Spark Blue™ 574 anti-mouse CD4

Isotype :

Rat IgG2b, κ

Other Names :

L3T4, T4

Size :

25 μg

Price (USD) :

132 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

SPKBL_574

Immunogen :

Mouse CTL clone V4

Application Notes :

Additional reported applications (for the relevant formats) include: blocking of CD4 + T cell activation 1,4,11 , thymocyte costimulation 3 , in vitro and in vivo depletion 2,5-8 , blocking of egg-sperm cell adhesion 1,4 , immunohistochemical staining of acetone-fixed frozen sections 9,10 , immunoprecipitation 1,2 , and spatial biology (IBEX) 12,13 . The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).

more info or order :

BioLegend product webpage