APC/Fire™ 750 anti-mouse CD4 | BioLegend 100459 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC/Fire™ 750 anti-mouse CD4

catalog :

100459

quantity :

25 μg

price :

85 USD

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

GK1.5

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:200; loading ...; fig s5a, s6a	Ansaldo E, Slayden L, Ching K, Koch M, Wolf N, Plichta D, et al. Akkermansia muciniphila induces intestinal adaptive immune responses during homeostasis. Science. 2019;364:1179-1184 pubmed publisher
flow cytometry; mouse; loading ...; fig 4b	Kirschbaum K, Sonner J, Zeller M, Deumelandt K, Bode J, Sharma R, et al. In vivo nanoparticle imaging of innate immune cells can serve as a marker of disease severity in a model of multiple sclerosis. Proc Natl Acad Sci U S A. 2016;113:13227-13232 pubmed
flow cytometry; mouse; fig 2	Huang M, Zhang W, Guo J, Wei X, Phiwpan K, Zhang J, et al. Improved Transgenic Mouse Model for Studying HLA Class I Antigen Presentation. Sci Rep. 2016;6:33612 pubmed publisher
flow cytometry; mouse; loading ...; fig 2a	Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
	Limon J, Tang J, Li D, Wolf A, Michelsen K, Funari V, et al. Malassezia Is Associated with Crohn's Disease and Exacerbates Colitis in Mouse Models. Cell Host Microbe. 2019;: pubmed publisher
	Abbott R, Lee J, Menis S, Skog P, Rossi M, Ota T, et al. Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens. Immunity. 2018;48:133-146.e6 pubmed publisher

product information

Antigen :

CD4

Apps. Abbrev. :

Flow Cytometry

Cat # :

100459

Clone :

GK1.5

Item :

APC/Fire™ 750 anti-mouse CD4

Isotype :

Rat IgG2b, κ

Other Names :

L3T4, T4

Size :

25 μg

Price (USD) :

85 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

APC_F750

Immunogen :

Mouse CTL clone V4

Application Notes :

Additional reported applications (for the relevant formats) include: blocking of CD4 + T cell activation 1,4,11 , thymocyte costimulation 3 , in vitro and in vivo depletion 2,5-8 , blocking of egg-sperm cell adhesion 1,4 , immunohistochemical staining of acetone-fixed frozen sections 9,10 , and immunoprecipitation 1,2 . The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).

more info or order :

BioLegend product webpage