product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Dazzle™ 594 anti-mouse CD4
catalog :
100455
quantity :
25 μg
price :
124 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
GK1.5
reactivity :
human, mouse
application :
immunohistochemistry, flow cytometry
more info or order :
citations: 17
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s1a, 4c
Aiken T, Erbe A, Zebertavage L, Komjathy D, Feils A, Rodriguez M, et al. Mechanism of effective combination radio-immunotherapy against 9464D-GD2, an immunologically cold murine neuroblastoma. J Immunother Cancer. 2022;10: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b, 4c
Seung H, Wröbel J, Wadle C, B xfc hler T, Chiang D, Rettkowski J, et al. P2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction. Basic Res Cardiol. 2022;117:16 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Petty A, Dai R, Lapalombella R, Baiocchi R, Benson D, Li Z, et al. Hedgehog-induced PD-L1 on tumor-associated macrophages is critical for suppression of tumor-infiltrating CD8+ T cell function. JCI Insight. 2021;6: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2f, s7c
Phan T, Schink L, Mann J, Merk V, Zwicky P, Mundt S, et al. Keratinocytes control skin immune homeostasis through de novo-synthesized glucocorticoids. Sci Adv. 2021;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3f
Li Z, Zhang H, Huang Y, Huang J, Sun P, Zhou N, et al. Autophagy deficiency promotes triple-negative breast cancer resistance to T cell-mediated cytotoxicity by blocking tenascin-C degradation. Nat Commun. 2020;11:3806 pubmed publisher
  • immunohistochemistry; mouse; fig 4h
Zheng D, Gao F, Cheng Q, Bao P, Dong X, Fan J, et al. A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11:1985 pubmed publisher
  • flow cytometry; human; loading ...; fig s7a
Binnewies M, Mujal A, Pollack J, Combes A, Hardison E, Barry K, et al. Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4+ T Cell Immunity. Cell. 2019;177:556-571.e16 pubmed publisher
  • flow cytometry; mouse; fig s1
Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Li Q, Zou J, Wang M, Ding X, Chepelev I, Zhou X, et al. Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T-cell differentiation. Nat Commun. 2014;5:5780 pubmed publisher
Lebratti T, Lim Y, Cofie A, Andhey P, Jiang X, Scott J, et al. A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection. elife. 2021;10: pubmed publisher
Fang H, Bonora G, Lewandowski J, Thakur J, Filippova G, Henikoff S, et al. Trans- and cis-acting effects of Firre on epigenetic features of the inactive X chromosome. Nat Commun. 2020;11:6053 pubmed publisher
Lewandowski J, Dumbovic G, Watson A, Hwang T, Jacobs Palmer E, Chang N, et al. The Tug1 lncRNA locus is essential for male fertility. Genome Biol. 2020;21:237 pubmed publisher
Iwanami N, Takeshita K, Lawir D, Suetake I, Tajima S, Sikora K, et al. Epigenetic Protection of Vertebrate Lymphoid Progenitor Cells by Dnmt1. iScience. 2020;23:101260 pubmed publisher
Siamishi I, Iwanami N, Clapes T, Trompouki E, O Meara C, Boehm T. Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles. Cell Rep. 2020;31:107756 pubmed publisher
Voeller J, Erbe A, Slowinski J, Rasmussen K, Carlson P, Hoefges A, et al. Combined innate and adaptive immunotherapy overcomes resistance of immunologically cold syngeneic murine neuroblastoma to checkpoint inhibition. J Immunother Cancer. 2019;7:344 pubmed publisher
Wu F, Xu P, Chow A, Man S, Kruger J, Khan K, et al. Pre- and post-operative anti-PD-L1 plus anti-angiogenic therapies in mouse breast or renal cancer models of micro- or macro-metastatic disease. Br J Cancer. 2019;120:196-206 pubmed publisher
van Nieuwenhuijze A, Burton O, Lemaitre P, Denton A, Cascalho A, Goodchild R, et al. Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol. 2018;9:2234 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
100455
Clone :
GK1.5
Item :
PE/Dazzle™ 594 anti-mouse CD4
Isotype :
Rat IgG2b, κ
Other Names :
L3T4, T4
Size :
25 μg
Price (USD) :
124 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE_DZL594
Immunogen :
Mouse CTL clone V4
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of CD4 + T cell activation 1,4,11 , thymocyte costimulation 3 , in vitro and in vivo depletion 2,5-8 , blocking of egg-sperm cell adhesion 1,4 , immunohistochemical staining of acetone-fixed frozen sections 9,10 , immunoprecipitation 1,2 , and spatial biology (IBEX) 12,13 . The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA