product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-mouse CD4
catalog :
100438
quantity :
500 μL
price :
374 USD
clonality :
monoclonal
host :
rat
conjugate :
BV421
clone name :
GK1.5
reactivity :
mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 72
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2b
Yang M, Long D, Hu L, Zhao Z, Li Q, Guo Y, et al. AIM2 deficiency in B cells ameliorates systemic lupus erythematosus by regulating Blimp-1-Bcl-6 axis-mediated B-cell differentiation. Signal Transduct Target Ther. 2021;6:341 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Yan C, Saleh N, Yang J, Nebhan C, Vilgelm A, Reddy E, et al. Novel induction of CD40 expression by tumor cells with RAS/RAF/PI3K pathway inhibition augments response to checkpoint blockade. Mol Cancer. 2021;20:85 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 5a
Li K, Yuan Z, Lyu J, Ahn E, Davis S, Ahmed R, et al. PD-1 suppresses TCR-CD8 cooperativity during T-cell antigen recognition. Nat Commun. 2021;12:2746 pubmed publisher
  • flow cytometry; mouse; fig 4f
Silva D, Yu S, Ulge U, Spangler J, Jude K, Labao Almeida C, et al. De novo design of potent and selective mimics of IL-2 and IL-15. Nature. 2019;565:186-191 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 3e
Rezende R, Lanser A, Rubino S, Kuhn C, Skillin N, Moreira T, et al. γδ T cells control humoral immune response by inducing T follicular helper cell differentiation. Nat Commun. 2018;9:3151 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s11
Foerster F, Boegel S, Heck R, Pickert G, R ssel N, Rosigkeit S, et al. Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells. Oncoimmunology. 2018;7:e1409929 pubmed publisher
Yi J, Tavana O, Li H, Wang D, Baer R, Gu W. Targeting USP2 regulation of VPRBP-mediated degradation of p53 and PD-L1 for cancer therapy. Nat Commun. 2023;14:1941 pubmed publisher
Liu P, Hou G, Kuang Y, Li L, Chen C, Yan B, et al. Lipidomic profiling reveals metabolic signatures in psoriatic skin lesions. Clin Immunol. 2023;246:109212 pubmed publisher
Quijano Rubio A, Bhuiyan A, Yang H, Leung I, Bello E, Ali L, et al. A split, conditionally active mimetic of IL-2 reduces the toxicity of systemic cytokine therapy. Nat Biotechnol. 2023;41:532-540 pubmed publisher
Whyte C, Tumes D, Liston A, Burton O. Do more with Less: Improving High Parameter Cytometry Through Overnight Staining. Curr Protoc. 2022;2:e589 pubmed publisher
Jeon Y, Kang H, Yang Y, Park D, Choi B, Kim J, et al. A Novel Selective Axl/Mer/CSF1R Kinase Inhibitor as a Cancer Immunotherapeutic Agent Targeting Both Immune and Tumor Cells in the Tumor Microenvironment. Cancers (Basel). 2022;14: pubmed publisher
Tajima M, Strober W. Evaluation of Glutaminolysis in T Cells. Curr Protoc. 2022;2:e540 pubmed publisher
Balakrishnan P, Ledezma D, Cano Mejia J, Andricovich J, Palmer E, Patel V, et al. CD137 agonist potentiates the abscopal efficacy of nanoparticle-based photothermal therapy for melanoma. Nano Res. 2022;15:2300-2314 pubmed publisher
Zhang J, Li F, Yin Y, Liu N, Zhu M, Zhang H, et al. Alpha radionuclide-chelated radioimmunotherapy promoters enable local radiotherapy/chemodynamic therapy to discourage cancer progression. Biomater Res. 2022;26:44 pubmed publisher
Liu T, Gonzalez De Los Santos F, Rinke A, Fang C, Flaherty K, Phan S. B7H3-dependent myeloid-derived suppressor cell recruitment and activation in pulmonary fibrosis. Front Immunol. 2022;13:901349 pubmed publisher
Li Y, Wang Z, Li F, Zhou L, Jiang Y, Yu Y, et al. Inhibition of myeloid-derived suppressor cell arginase-1 production enhances T-cell-based immunotherapy against Cryptococcus neoformans infection. Nat Commun. 2022;13:4074 pubmed publisher
Blanco L, Patiño Martínez E, Nakabo S, Zhang M, Pedersen H, Wang X, et al. Modulation of the Itaconate Pathway Attenuates Murine Lupus. Arthritis Rheumatol. 2022;74:1971-1983 pubmed publisher
Li H, Wang Z, Fang X, Zeng W, Yang Y, Jin L, et al. Poroptosis: A form of cell death depending on plasma membrane nanopores formation. iScience. 2022;25:104481 pubmed publisher
Tzeng T, Chai K, Shen K, Yu C, Yang S, Huang W, et al. A DNA vaccine candidate delivered by an electroacupuncture machine provides protective immunity against SARS-CoV-2 infection. NPJ Vaccines. 2022;7:60 pubmed publisher
Johansen A, Carretta M, Thorseth M, Khan S, Fj xe6 stad K, Br xf8 chner C, et al. Chitooligosaccharides Improve the Efficacy of Checkpoint Inhibitors in a Mouse Model of Lung Cancer. Pharmaceutics. 2022;14: pubmed publisher
Takahashi F, Zhang C, Hohjoh H, Raveney B, Yamamura T, Hayashi N, et al. Immune-mediated neurodegenerative trait provoked by multimodal derepression of long-interspersed nuclear element-1. iScience. 2022;25:104278 pubmed publisher
Luo J, Wang X, Shi Z, Zeng Y, He L, Cao J, et al. Enhancement of antitumor immunotherapy using mitochondria-targeted cancer cell membrane-biomimetic MOF-mediated sonodynamic therapy and checkpoint blockade immunotherapy. J Nanobiotechnology. 2022;20:228 pubmed publisher
Qi X, Wu F, Kim S, Kaifi J, Kimchi E, Snyder H, et al. Nanoliposome C6-Ceramide in combination with anti-CTLA4 antibody improves anti-tumor immunity in hepatocellular cancer. FASEB J. 2022;36:e22250 pubmed publisher
Yeh C, Finney J, Okada T, Kurosaki T, Kelsoe G. Primary germinal center-resident T follicular helper cells are a physiologically distinct subset of CXCR5hiPD-1hi T follicular helper cells. Immunity. 2022;55:272-289.e7 pubmed publisher
Yan C, Chen S, Ayers G, Nebhan C, Roland J, Weiss V, et al. Proximity of immune and tumor cells underlies response to BRAF/MEK-targeted therapies in metastatic melanoma patients. NPJ Precis Oncol. 2022;6:6 pubmed publisher
Georgiadou A, Dunican C, Soro Barrio P, Lee H, Kaforou M, Cunnington A. Comparative transcriptomic analysis reveals translationally relevant processes in mouse models of malaria. elife. 2022;11: pubmed publisher
Ercoli G, Ramos Sevillano E, Pearce E, Ragab S, Goldblatt D, Weckbecker G, et al. Maintained partial protection against Streptococcus pneumoniae despite B-cell depletion in mice vaccinated with a pneumococcal glycoconjugate vaccine. Clin Transl Immunology. 2022;11:e1366 pubmed publisher
Alberti D, Guarniero M, Maciola A, Dotta E, Pasqual G. Engineering Ligand and Receptor Pairs with LIPSTIC to Track Cell-Cell Interactions. Curr Protoc. 2021;1:e311 pubmed publisher
Heil J, Olsavszky V, Busch K, Klapproth K, de la Torre C, Sticht C, et al. Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation. Nat Commun. 2021;12:6963 pubmed publisher
Gabriely G, Ma D, Siddiqui S, Sun L, Skillin N, Abou El Hassan H, et al. Myeloid cell subsets that express latency-associated peptide promote cancer growth by modulating T cells. iScience. 2021;24:103347 pubmed publisher
Gómez Diaz C, Jönsson G, Schodl K, Deszcz L, Bestehorn A, Eislmayr K, et al. The ubiquitin ligase HOIL-1L regulates immune responses by interacting with linear ubiquitin chains. iScience. 2021;24:103241 pubmed publisher
Jütte B, Krollmann C, Cieslak K, Koerber R, Boor P, Graef C, et al. Intercellular cGAMP transmission induces innate immune activation and tissue inflammation in Trex1 deficiency. iScience. 2021;24:102833 pubmed publisher
Super M, Doherty E, Cartwright M, Seiler B, Langellotto F, Dimitrakakis N, et al. Biomaterial vaccines capturing pathogen-associated molecular patterns protect against bacterial infections and septic shock. Nat Biomed Eng. 2021;: pubmed publisher
Marinescu C, Preda M, Burlacu A. A procedure for in vitro evaluation of the immunosuppressive effect of mouse mesenchymal stem cells on activated T cell proliferation. Stem Cell Res Ther. 2021;12:319 pubmed publisher
Mittal A, Wang M, Vidyarthi A, Yanez D, Pizzurro G, Thakral D, et al. Topical arginase inhibition decreases growth of cutaneous squamous cell carcinoma. Sci Rep. 2021;11:10731 pubmed publisher
Lu Y, Li Y, Liu Q, Tian N, Du P, Zhu F, et al. MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment. Gastroenterology. 2021;161:575-591.e16 pubmed publisher
Bhatt D, Kang B, Sawant D, Zheng L, PEREZ K, Huang Z, et al. STARTRAC analyses of scRNAseq data from tumor models reveal T cell dynamics and therapeutic targets. J Exp Med. 2021;218: pubmed publisher
Garo L, Ajay A, Fujiwara M, Gabriely G, Raheja R, Kuhn C, et al. MicroRNA-146a limits tumorigenic inflammation in colorectal cancer. Nat Commun. 2021;12:2419 pubmed publisher
Uzhachenko R, Bharti V, Ouyang Z, Blevins A, Mont S, Saleh N, et al. Metabolic modulation by CDK4/6 inhibitor promotes chemokine-mediated recruitment of T cells into mammary tumors. Cell Rep. 2021;35:108944 pubmed publisher
Boulch M, Cazaux M, Loe Mie Y, Thibaut R, Corre B, Lemaitre F, et al. A cross-talk between CAR T cell subsets and the tumor microenvironment is essential for sustained cytotoxic activity. Sci Immunol. 2021;6: pubmed publisher
Kiuchi M, Onodera A, Kokubo K, Ichikawa T, Morimoto Y, Kawakami E, et al. The Cxxc1 subunit of the Trithorax complex directs epigenetic licensing of CD4+ T cell differentiation. J Exp Med. 2021;218: pubmed publisher
Li J, Zhang L, Zheng Y, Shao R, Liang Q, Yu W, et al. BAD inactivation exacerbates rheumatoid arthritis pathology by promoting survival of sublining macrophages. elife. 2020;9: pubmed publisher
Zhu X, Chudnovskiy A, Baudrier L, Prizer B, Liu Y, Ostendorf B, et al. Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells. Cell Metab. 2021;33:211-221.e6 pubmed publisher
Perner C, Flayer C, Zhu X, Aderhold P, Dewan Z, Voisin T, et al. Substance P Release by Sensory Neurons Triggers Dendritic Cell Migration and Initiates the Type-2 Immune Response to Allergens. Immunity. 2020;53:1063-1077.e7 pubmed publisher
Hassan A, Kafai N, Dmitriev I, Fox J, Smith B, Harvey I, et al. A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2. Cell. 2020;183:169-184.e13 pubmed publisher
Alam I, Simonetta F, Scheller L, Mayer A, Murty S, Vermesh O, et al. Visualization of Activated T Cells by OX40-ImmunoPET as a Strategy for Diagnosis of Acute Graft-versus-Host Disease. Cancer Res. 2020;80:4780-4790 pubmed publisher
Penkert R, Cortez V, Karlsson E, Livingston B, Surman S, Li Y, et al. Vitamin A Corrects Tissue Deficits in Diet-Induced Obese Mice and Reduces Influenza Infection After Vaccination and Challenge. Obesity (Silver Spring). 2020;28:1631-1636 pubmed publisher
Monaghan K, Farris B, Zheng W, Wan E. Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment. J Vis Exp. 2020;: pubmed publisher
Alam Z, Devalaraja S, Li M, To T, Folkert I, Mitchell Velasquez E, et al. Counter Regulation of Spic by NF-κB and STAT Signaling Controls Inflammation and Iron Metabolism in Macrophages. Cell Rep. 2020;31:107825 pubmed publisher
Runge E, Iyer A, Setter D, Kennedy F, Sanders V, Jones K. CD4+ T cell expression of the IL-10 receptor is necessary for facial motoneuron survival after axotomy. J Neuroinflammation. 2020;17:121 pubmed publisher
Han Y, Jia Q, Jahani P, Hurrell B, Pan C, Huang P, et al. Genome-wide analysis highlights contribution of immune system pathways to the genetic architecture of asthma. Nat Commun. 2020;11:1776 pubmed publisher
Devalaraja S, To T, Folkert I, Natesan R, Alam M, Li M, et al. Tumor-Derived Retinoic Acid Regulates Intratumoral Monocyte Differentiation to Promote Immune Suppression. Cell. 2020;180:1098-1114.e16 pubmed publisher
Flamar A, Klose C, Moeller J, Mahlakõiv T, Bessman N, Zhang W, et al. Interleukin-33 Induces the Enzyme Tryptophan Hydroxylase 1 to Promote Inflammatory Group 2 Innate Lymphoid Cell-Mediated Immunity. Immunity. 2020;52:606-619.e6 pubmed publisher
Annu K, Cline C, Yasuda K, Ganguly S, Pesch A, Cooper B, et al. Role of Vitamins A and D in BCR-ABL Arf-/- Acute Lymphoblastic Leukemia. Sci Rep. 2020;10:2359 pubmed publisher
Mulas F, Wang X, Song S, Nishanth G, Yi W, Brunn A, et al. The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13. Cell Mol Immunol. 2020;: pubmed publisher
Steinmann S, Schoedsack M, Heinrich F, Breda P, Ochel A, Tiegs G, et al. Hepatic ILC2 activity is regulated by liver inflammation-induced cytokines and effector CD4+ T cells. Sci Rep. 2020;10:1071 pubmed publisher
Fukushima T, Tanaka Y, Hamey F, Chang C, Oki T, Asada S, et al. Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium. Cell Rep. 2019;29:4144-4158.e7 pubmed publisher
Etxeberria I, Bolaños E, Quetglas J, Gros A, Villanueva A, Palomero J, et al. Intratumor Adoptive Transfer of IL-12 mRNA Transiently Engineered Antitumor CD8+ T Cells. Cancer Cell. 2019;36:613-629.e7 pubmed publisher
Maluski M, Ghosh A, Herbst J, Scholl V, Baumann R, Huehn J, et al. Chimeric antigen receptor-induced BCL11B suppression propagates NK-like cell development. J Clin Invest. 2019;129:5108-5122 pubmed publisher
Yue X, Lio C, Samaniego Castruita D, Li X, Rao A. Loss of TET2 and TET3 in regulatory T cells unleashes effector function. Nat Commun. 2019;10:2011 pubmed publisher
Huai W, Liu X, Wang C, Zhang Y, Chen X, Chen X, et al. KAT8 selectively inhibits antiviral immunity by acetylating IRF3. J Exp Med. 2019;216:772-785 pubmed publisher
Goswami M, Wang X, Zhang P, Xiao W, Karlen S, Li Y, et al. Novel window for cancer nanotheranostics: non-invasive ocular assessments of tumor growth and nanotherapeutic treatment efficacy in vivo. Biomed Opt Express. 2019;10:151-166 pubmed publisher
Garg G, Muschaweckh A, Moreno H, Vasanthakumar A, Floess S, Lepennetier G, et al. Blimp1 Prevents Methylation of Foxp3 and Loss of Regulatory T Cell Identity at Sites of Inflammation. Cell Rep. 2019;26:1854-1868.e5 pubmed publisher
Bennion B, Ingle H, Ai T, Miner C, Platt D, Smith A, et al. A Human Gain-of-Function STING Mutation Causes Immunodeficiency and Gammaherpesvirus-Induced Pulmonary Fibrosis in Mice. J Virol. 2019;93: pubmed publisher
Cignarella F, Cantoni C, Ghezzi L, Salter A, Dorsett Y, Chen L, et al. Intermittent Fasting Confers Protection in CNS Autoimmunity by Altering the Gut Microbiota. Cell Metab. 2018;27:1222-1235.e6 pubmed publisher
Xu J, Mathur J, Vessieres E, Hammack S, Nonomura K, Favre J, et al. GPR68 Senses Flow and Is Essential for Vascular Physiology. Cell. 2018;173:762-775.e16 pubmed publisher
Angela M, Endo Y, Asou H, Yamamoto T, Tumes D, Tokuyama H, et al. Fatty acid metabolic reprogramming via mTOR-mediated inductions of PPAR? directs early activation of T cells. Nat Commun. 2016;7:13683 pubmed publisher
Cabrera Perez J, Babcock J, Dileepan T, Murphy K, Kucaba T, Badovinac V, et al. Gut Microbial Membership Modulates CD4 T Cell Reconstitution and Function after Sepsis. J Immunol. 2016;197:1692-8 pubmed publisher
Gorman M, Poddar S, Farzan M, Diamond M. The Interferon-Stimulated Gene Ifitm3 Restricts West Nile Virus Infection and Pathogenesis. J Virol. 2016;90:8212-25 pubmed publisher
Inoue M, Shinohara M. Cutting edge: Role of osteopontin and integrin αv in T cell-mediated anti-inflammatory responses in endotoxemia. J Immunol. 2015;194:5595-8 pubmed publisher
Cabrera Perez J, Condotta S, James B, Kashem S, Brincks E, Rai D, et al. Alterations in antigen-specific naive CD4 T cell precursors after sepsis impairs their responsiveness to pathogen challenge. J Immunol. 2015;194:1609-20 pubmed publisher
Van Acker A, Filtjens J, Van Welden S, Taveirne S, Van Ammel E, Vanhees M, et al. Ly49E expression on CD8αα-expressing intestinal intraepithelial lymphocytes plays no detectable role in the development and progression of experimentally induced inflammatory bowel diseases. PLoS ONE. 2014;9:e110015 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
FC, ICC, IHC-F
Cat # :
100438
Clone :
GK1.5
Item :
Brilliant Violet 421™ anti-mouse CD4
Isotype :
Rat IgG2b, κ
Other Names :
L3T4, T4
Size :
500 μL
Price (USD) :
374 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV421
Immunogen :
Mouse CTL clone V4
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of CD4^+ T cell activation^1,4,11, thymocyte costimulation^3, in vitro and in vivo depletion^2,5-8, blocking of egg-sperm cell adhesion^1,4, immunohistochemical staining of acetone-fixed frozen sections^9,10, immunoprecipitation^1,2, and spatial biology (IBEX)^12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA