product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP/Cyanine5.5 anti-mouse CD4
catalog :
100434
quantity :
100 μg
price :
323 USD
clonality :
monoclonal
host :
rat
conjugate :
PerCP/Cy5.5
clone name :
GK1.5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 130
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4b
Zhao F, Zhang C, Li G, Zheng H, Gu L, Zhou H, et al. A role for whey acidic protein four-disulfide-core 12 (WFDC12) in the pathogenesis and development of psoriasis disease. Front Immunol. 2022;13:873720 pubmed publisher
  • flow cytometry; mouse; 1:800; loading ...; fig s2a
Clayer E, Frank D, Anderton H, Zhang S, Kueh A, Heim V, et al. ZC3H12C expression in dendritic cells is necessary to prevent lymphadenopathy of skin-draining lymph nodes. Immunol Cell Biol. 2022;100:160-173 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Yang M, Long D, Hu L, Zhao Z, Li Q, Guo Y, et al. AIM2 deficiency in B cells ameliorates systemic lupus erythematosus by regulating Blimp-1-Bcl-6 axis-mediated B-cell differentiation. Signal Transduct Target Ther. 2021;6:341 pubmed publisher
  • flow cytometry; mouse; 1:250; loading ...; fig s8b
Torretta S, Scagliola A, Ricci L, Mainini F, Di Marco S, Cuccovillo I, et al. D-mannose suppresses macrophage IL-1β production. Nat Commun. 2020;11:6343 pubmed publisher
  • flow cytometry; mouse; fig 2a
Ishizuka J, Manguso R, Cheruiyot C, Bi K, Panda A, Iracheta Vellve A, et al. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature. 2019;565:43-48 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Webster P, Dawes J, Dewchand H, Takacs K, Iadarola B, Bolt B, et al. Subclonal mutation selection in mouse lymphomagenesis identifies known cancer loci and suggests novel candidates. Nat Commun. 2018;9:2649 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Anderson A, Baranowska Hustad M, Braathen R, Grodeland G, Bogen B. Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza. J Immunol. 2018;200:2057-2066 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, et al. BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J Exp Med. 2017;214:1313-1331 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Fisher S, Aston W, Chee J, Khong A, Cleaver A, Solin J, et al. Transient Treg depletion enhances therapeutic anti-cancer vaccination. Immun Inflamm Dis. 2017;5:16-28 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Wei Y, Lu C, Chen J, Cui G, Wang L, Yu T, et al. High salt diet stimulates gut Th17 response and exacerbates TNBS-induced colitis in mice. Oncotarget. 2017;8:70-82 pubmed publisher
  • flow cytometry; mouse; fig 3
Li J, Chassaing B, Tyagi A, Vaccaro C, Luo T, Adams J, et al. Sex steroid deficiency-associated bone loss is microbiota dependent and prevented by probiotics. J Clin Invest. 2016;126:2049-63 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 9
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Zhao Q, Yu J, Zhou H, Wang X, Zhang C, Hu J, et al. Intestinal dysbiosis exacerbates the pathogenesis of psoriasis-like phenotype through changes in fatty acid metabolism. Signal Transduct Target Ther. 2023;8:40 pubmed publisher
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product information
Antigen :
CD4
Apps. Abbrev. :
FC
Cat # :
100434
Clone :
GK1.5
Item :
PerCP/Cyanine5.5 anti-mouse CD4
Isotype :
Rat IgG2b, κ
Other Names :
L3T4, T4
Size :
100 μg
Price (USD) :
323 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PERCP_CYANINE5.5
Immunogen :
Mouse CTL clone V4
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of CD4^+ T cell activation^1,4,11, thymocyte costimulation^3, in vitro and in vivo depletion^2,5-8, blocking of egg-sperm cell adhesion^1,4, immunohistochemical staining of acetone-fixed frozen sections^9,10, immunoprecipitation^1,2, and spatial biology (IBEX)^12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA