product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-mouse CD4
catalog :
100427
quantity :
25 μg
price :
94 USD
clonality :
monoclonal
host :
rat
conjugate :
Pacific Blue
clone name :
GK1.5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 56
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig 2s3e
Glassman C, Su L, Majri Morrison S, Winkelmann H, Mo F, Li P, et al. Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:500; fig 1j
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; 5 ug/ml; loading ...; fig 2j, 3a, s3a, s12r
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s16c
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Zhang S, Takaku M, Zou L, Gu A, Chou W, Zhang G, et al. Reversing SKI-SMAD4-mediated suppression is essential for TH17 cell differentiation. Nature. 2017;551:105-109 pubmed publisher
  • flow cytometry; mouse; loading ...; fig S2
Uhde A, Herder V, Akram Khan M, Ciurkiewicz M, Schaudien D, Teich R, et al. Viral Infection of the Central Nervous System Exacerbates Interleukin-10 Receptor Deficiency-Mediated Colitis in SJL Mice. PLoS ONE. 2016;11:e0161883 pubmed publisher
  • flow cytometry; mouse; fig s3
Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
Wang R, Chen J, Wang W, Zhao Z, Wang H, Liu S, et al. CD40L-armed oncolytic herpes simplex virus suppresses pancreatic ductal adenocarcinoma by facilitating the tumor microenvironment favorable to cytotoxic T cell response in the syngeneic mouse model. J Immunother Cancer. 2022;10: pubmed publisher
Lebratti T, Lim Y, Cofie A, Andhey P, Jiang X, Scott J, et al. A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection. elife. 2021;10: pubmed publisher
Lutes L, Steier Z, McIntyre L, Pandey S, Kaminski J, Hoover A, et al. T cell self-reactivity during thymic development dictates the timing of positive selection. elife. 2021;10: pubmed publisher
Daneshmandi S, Cassel T, Lin P, Higashi R, Wulf G, Boussiotis V, et al. Blockade of 6-phosphogluconate dehydrogenase generates CD8+ effector T cells with enhanced anti-tumor function. Cell Rep. 2021;34:108831 pubmed publisher
Heyde A, Rohde D, McAlpine C, Zhang S, Hoyer F, Gerold J, et al. Increased stem cell proliferation in atherosclerosis accelerates clonal hematopoiesis. Cell. 2021;184:1348-1361.e22 pubmed publisher
Nayak R, Alexander M, Deshpande I, Stapleton Gray K, Rimal B, Patterson A, et al. Methotrexate impacts conserved pathways in diverse human gut bacteria leading to decreased host immune activation. Cell Host Microbe. 2021;29:362-377.e11 pubmed publisher
Wu B, Zhang S, Guo Z, Bi Y, Zhou M, Li P, et al. The TGF-β superfamily cytokine Activin-A is induced during autoimmune neuroinflammation and drives pathogenic Th17 cell differentiation. Immunity. 2021;54:308-323.e6 pubmed publisher
Zhang L, Wang W, Wang R, Zhang N, Shang H, Bi Y, et al. Reshaping the Immune Microenvironment by Oncolytic Herpes Simplex Virus in Murine Pancreatic Ductal Adenocarcinoma. Mol Ther. 2021;29:744-761 pubmed publisher
Jiang L, Wang Y, Zhao J, Uehara M, Hou Q, Kasinath V, et al. Direct Tumor Killing and Immunotherapy through Anti-SerpinB9 Therapy. Cell. 2020;183:1219-1233.e18 pubmed publisher
Simoni L, Presumey J, van der Poel C, Castrillón C, Chang S, Utz P, et al. Complement C4A Regulates Autoreactive B Cells in Murine Lupus. Cell Rep. 2020;33:108330 pubmed publisher
Bogie J, Grajchen E, Wouters E, Broux B, Stinissen P, Van Wijmeersch B, et al. CNS delivery of anti-CD52 antibodies modestly reduces disease severity in an animal model for multiple sclerosis. Ther Adv Chronic Dis. 2020;11:2040622320947378 pubmed publisher
Alissafi T, Kalafati L, Lazari M, Filia A, Kloukina I, Manifava M, et al. Mitochondrial Oxidative Damage Underlies Regulatory T Cell Defects in Autoimmunity. Cell Metab. 2020;32:591-604.e7 pubmed publisher
Manion K, Baglaenko Y, Chang N, Talaei N, Wither J. Impaired B cell anergy is not sufficient to breach tolerance to nuclear antigen in Vκ8/3H9 lupus-prone mice. PLoS ONE. 2020;15:e0236664 pubmed publisher
Dumas A, Pomella N, Rosser G, Guglielmi L, Vinel C, Millner T, et al. Microglia promote glioblastoma via mTOR-mediated immunosuppression of the tumour microenvironment. EMBO J. 2020;39:e103790 pubmed publisher
Ang Q, Alexander M, Newman J, Tian Y, Cai J, Upadhyay V, et al. Ketogenic Diets Alter the Gut Microbiome Resulting in Decreased Intestinal Th17 Cells. Cell. 2020;181:1263-1275.e16 pubmed publisher
Ma Y, Yang Q, Fan M, Zhang L, Gu Y, Jia W, et al. Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation. Cell Prolif. 2020;53:e12802 pubmed publisher
Brenner E, Schörg B, Ahmetlić F, Wieder T, Hilke F, Simon N, et al. Cancer immune control needs senescence induction by interferon-dependent cell cycle regulator pathways in tumours. Nat Commun. 2020;11:1335 pubmed publisher
Tian D, Yang L, Wang S, Zhu Y, Shi W, Zhang C, et al. Double negative T cells mediate Lag3-dependent antigen-specific protection in allergic asthma. Nat Commun. 2019;10:4246 pubmed publisher
Zheng X, Oduro J, Boehme J, Borkner L, Ebensen T, Heise U, et al. Mucosal CD8+ T cell responses induced by an MCMV based vaccine vector confer protection against influenza challenge. PLoS Pathog. 2019;15:e1008036 pubmed publisher
Kobayashi S, Sakurai T, So T, Shiota Y, Asao A, Phung H, et al. TNF Receptor-Associated Factor 5 Limits Function of Plasmacytoid Dendritic Cells by Controlling IFN Regulatory Factor 5 Expression. J Immunol. 2019;203:1447-1456 pubmed publisher
Vono M, Eberhardt C, Auderset F, Mastelic Gavillet B, Lemeille S, Christensen D, et al. Maternal Antibodies Inhibit Neonatal and Infant Responses to Vaccination by Shaping the Early-Life B Cell Repertoire within Germinal Centers. Cell Rep. 2019;28:1773-1784.e5 pubmed publisher
Dangaj D, Bruand M, Grimm A, Ronet C, Barras D, Duttagupta P, et al. Cooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors. Cancer Cell. 2019;35:885-900.e10 pubmed publisher
Zhou J, Huang S, Wang Z, Huang J, Xu L, Tang X, et al. Targeting EZH2 histone methyltransferase activity alleviates experimental intestinal inflammation. Nat Commun. 2019;10:2427 pubmed publisher
Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
Zhao J, Wen X, Tian L, Li T, Xu C, Wen X, et al. Irreversible electroporation reverses resistance to immune checkpoint blockade in pancreatic cancer. Nat Commun. 2019;10:899 pubmed publisher
Wu B, Zhang S, Guo Z, Wang G, Zhang G, Xie L, et al. RAS P21 Protein Activator 3 (RASA3) Specifically Promotes Pathogenic T Helper 17 Cell Generation by Repressing T-Helper-2-Cell-Biased Programs. Immunity. 2018;49:886-898.e5 pubmed publisher
Wang Z, Yin W, Zhu L, Li J, Yao Y, Chen F, et al. Iron Drives T Helper Cell Pathogenicity by Promoting RNA-Binding Protein PCBP1-Mediated Proinflammatory Cytokine Production. Immunity. 2018;49:80-92.e7 pubmed publisher
Lu Y, Wang Q, Xue G, Bi E, Ma X, Wang A, et al. Th9 Cells Represent a Unique Subset of CD4+ T Cells Endowed with the Ability to Eradicate Advanced Tumors. Cancer Cell. 2018;33:1048-1060.e7 pubmed publisher
Liu S, Xia Q, Wu X, Sun F, Hu Q, Wu J, et al. Stimulator of Interferon Genes in Classical Dendritic Cells Controls Mucosal Th17 Responses to Cyclic Dinucleotides for Host Defenses Against Microbial Infections in Gut. Front Immunol. 2018;9:1085 pubmed publisher
Hsieh W, Hsu T, Chang Y, Lai M. IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells. Nat Commun. 2018;9:463 pubmed publisher
Vacca M, Böhme J, Zambetti L, Khameneh H, Paleja B, Laudisi F, et al. NLRP10 Enhances CD4+ T-Cell-Mediated IFN? Response via Regulation of Dendritic Cell-Derived IL-12 Release. Front Immunol. 2017;8:1462 pubmed publisher
Thwe P, Pelgrom L, Cooper R, Beauchamp S, Reisz J, D Alessandro A, et al. Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses. Cell Metab. 2017;26:558-567.e5 pubmed publisher
Borkner L, Sitnik K, Dekhtiarenko I, Pulm A, Tao R, Drexler I, et al. Immune Protection by a Cytomegalovirus Vaccine Vector Expressing a Single Low-Avidity Epitope. J Immunol. 2017;199:1737-1747 pubmed publisher
Dekhtiarenko I, Ratts R, Blatnik R, Lee L, Fischer S, Borkner L, et al. Peptide Processing Is Critical for T-Cell Memory Inflation and May Be Optimized to Improve Immune Protection by CMV-Based Vaccine Vectors. PLoS Pathog. 2016;12:e1006072 pubmed publisher
Miyauchi K, Sugimoto Ishige A, Harada Y, Adachi Y, Usami Y, Kaji T, et al. Protective neutralizing influenza antibody response in the absence of T follicular helper cells. Nat Immunol. 2016;17:1447-1458 pubmed publisher
Cerina M, Narayanan V, Göbel K, Bittner S, Ruck T, Meuth P, et al. The quality of cortical network function recovery depends on localization and degree of axonal demyelination. Brain Behav Immun. 2017;59:103-117 pubmed publisher
Nicolay N, Rühle A, Perez R, Trinh T, Sisombath S, Weber K, et al. Mesenchymal stem cells are sensitive to bleomycin treatment. Sci Rep. 2016;6:26645 pubmed publisher
Baglaenko Y, Manion K, Chang N, Gracey E, Loh C, Wither J. IL-10 Production Is Critical for Sustaining the Expansion of CD5+ B and NKT Cells and Restraining Autoantibody Production in Congenic Lupus-Prone Mice. PLoS ONE. 2016;11:e0150515 pubmed publisher
Guo Z, Kong Q, Liu C, Zhang S, Zou L, Yan F, et al. DCAF1 controls T-cell function via p53-dependent and -independent mechanisms. Nat Commun. 2016;7:10307 pubmed publisher
Maruyama T. TGF-β-induced IκB-ζ controls Foxp3 gene expression. Biochem Biophys Res Commun. 2015;464:586-9 pubmed publisher
Price J, Hotta Iwamura C, Zhao Y, Beauchamp N, Tarbell K. DCIR2+ cDC2 DCs and Zbtb32 Restore CD4+ T-Cell Tolerance and Inhibit Diabetes. Diabetes. 2015;64:3521-31 pubmed publisher
Maruyama T, Kobayashi S, Ogasawara K, Yoshimura A, Chen W, Muta T. Control of IFN-γ production and regulatory function by the inducible nuclear protein IκB-ζ in T cells. J Leukoc Biol. 2015;98:385-93 pubmed publisher
Madireddi S, Eun S, Lee S, Nemčovičová I, Mehta A, Zajonc D, et al. Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies. J Exp Med. 2014;211:1433-48 pubmed publisher
Sawant D, Gravano D, Vogel P, Giacomin P, Artis D, Vignali D. Regulatory T cells limit induction of protective immunity and promote immune pathology following intestinal helminth infection. J Immunol. 2014;192:2904-12 pubmed publisher
Eitas T, Chou W, Wen H, Gris D, Robbins G, Brickey J, et al. The nucleotide-binding leucine-rich repeat (NLR) family member NLRX1 mediates protection against experimental autoimmune encephalomyelitis and represses macrophage/microglia-induced inflammation. J Biol Chem. 2014;289:4173-9 pubmed publisher
Wong L, HATFIELD J, Brown M. Ikaros sets the potential for Th17 lineage gene expression through effects on chromatin state in early T cell development. J Biol Chem. 2013;288:35170-9 pubmed publisher
Morawski P, Mehra P, Chen C, Bhatti T, Wells A. Foxp3 protein stability is regulated by cyclin-dependent kinase 2. J Biol Chem. 2013;288:24494-502 pubmed publisher
Dekhtiarenko I, Jarvis M, Ruzsics Z, Cicin Sain L. The context of gene expression defines the immunodominance hierarchy of cytomegalovirus antigens. J Immunol. 2013;190:3399-409 pubmed publisher
Teo T, Lum F, Claser C, Lulla V, Lulla A, Merits A, et al. A pathogenic role for CD4+ T cells during Chikungunya virus infection in mice. J Immunol. 2013;190:259-69 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
100427
Clone :
GK1.5
Item :
Pacific Blue™ anti-mouse CD4
Isotype :
Rat IgG2b, κ
Other Names :
L3T4, T4
Size :
25 μg
Price (USD) :
94 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PB
Immunogen :
Mouse CTL clone V4
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of CD4 + T cell activation 1,4,11 , thymocyte costimulation 3 , in vitro and in vivo depletion 2,5-8 , blocking of egg-sperm cell adhesion 1,4 , immunohistochemical staining of acetone-fixed frozen sections 9,10 , immunoprecipitation 1,2 , and spatial biology (IBEX) 12,13 . The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA