product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-mouse CD4
catalog :
100423
quantity :
100 μg
price :
229 USD
clonality :
monoclonal
host :
rat
conjugate :
AF488
clone name :
GK1.5
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 112
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; fig 2b
Tanaka Y, Onozato M, Mikami T, Kohwi Shigematsu T, Fukushima T, Kondo M. Increased Indoleamine 2,3-Dioxygenase Levels at the Onset of Sjögren's Syndrome in SATB1-Conditional Knockout Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig s2d
Rizvi Z, Dalal R, Sadhu S, Kumar Y, Kumar S, Gupta S, et al. High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity. Sci Adv. 2021;7:eabg5016 pubmed publisher
  • flow cytometry; mouse; fig 3
Forman R, Logunova L, Smith H, Wemyss K, Mair I, Boon L, et al. Trichuris muris infection drives cell-intrinsic IL4R alpha independent colonic RELMα+ macrophages. PLoS Pathog. 2021;17:e1009768 pubmed publisher
  • flow cytometry; mouse; loading ...
Mathä L, Romera Hernandez M, Steer C, Yin Y, Orangi M, Shim H, et al. Migration of Lung Resident Group 2 Innate Lymphoid Cells Link Allergic Lung Inflammation and Liver Immunity. Front Immunol. 2021;12:679509 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a, s2b
Toyama S, Moniaga C, Nakae S, Kurosawa M, Ogawa H, Tominaga M, et al. Regulatory T Cells Exhibit Interleukin-33-Dependent Migratory Behavior during Skin Barrier Disruption. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Gvozdeva O, Achasova K, Litvinova N, Kozhevnikova E, Litvinova E. Female Scent Activated Expression of Arginase1 and Inducible NO-Synthetase in Lung of BALB/c Male Mice. Animals (Basel). 2021;11: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6a
Innamarato P, Morse J, Mackay A, Asby S, Beatty M, Blauvelt J, et al. Intralesional injection of rose bengal augments the efficacy of gemcitabine chemotherapy against pancreatic tumors. BMC Cancer. 2021;21:756 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s22b
Ho D, Tsui Y, Chan L, Sze K, Zhang X, Cheu J, et al. Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma. Nat Commun. 2021;12:3684 pubmed publisher
  • flow cytometry; mouse; fig 4b
Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1s2a
Lebratti T, Lim Y, Cofie A, Andhey P, Jiang X, Scott J, et al. A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 5a
Li K, Yuan Z, Lyu J, Ahn E, Davis S, Ahmed R, et al. PD-1 suppresses TCR-CD8 cooperativity during T-cell antigen recognition. Nat Commun. 2021;12:2746 pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig s4a
Lin Q, Rong L, Jia X, Li R, Yu B, Hu J, et al. IFN-γ-dependent NK cell activation is essential to metastasis suppression by engineered Salmonella. Nat Commun. 2021;12:2537 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a, 5b, 5f
Reis M, Willis G, Fernandez Gonzalez A, Yeung V, Taglauer E, Magaletta M, et al. Mesenchymal Stromal Cell-Derived Extracellular Vesicles Restore Thymic Architecture and T Cell Function Disrupted by Neonatal Hyperoxia. Front Immunol. 2021;12:640595 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 1b
Zhang S, Li L, Xie D, Reddy S, Sleasman J, Ma L, et al. Regulation of Intrinsic and Bystander T Follicular Helper Cell Differentiation and Autoimmunity by Tsc1. Front Immunol. 2021;12:620437 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 7b
Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Petty A, Dai R, Lapalombella R, Baiocchi R, Benson D, Li Z, et al. Hedgehog-induced PD-L1 on tumor-associated macrophages is critical for suppression of tumor-infiltrating CD8+ T cell function. JCI Insight. 2021;6: pubmed publisher
  • flow cytometry; mouse; 1:50; fig 7
Mpekris F, Panagi M, Voutouri C, Martin J, Samuel R, Takahashi S, et al. Normalizing the Microenvironment Overcomes Vessel Compression and Resistance to Nano-immunotherapy in Breast Cancer Lung Metastasis. Adv Sci (Weinh). 2021;8:2001917 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3e
Break T, Oikonomou V, Dutzan N, Desai J, Swidergall M, Freiwald T, et al. Aberrant type 1 immunity drives susceptibility to mucosal fungal infections. Science. 2021;371: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2g
Sanchez Felipe L, Vercruysse T, Sharma S, Ma J, Lemmens V, Van Looveren D, et al. A single-dose live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate. Nature. 2021;590:320-325 pubmed publisher
  • flow cytometry; mouse; 1:500; fig 1j
Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
  • flow cytometry; mouse; 1:250; loading ...; fig s8b
Torretta S, Scagliola A, Ricci L, Mainini F, Di Marco S, Cuccovillo I, et al. D-mannose suppresses macrophage IL-1β production. Nat Commun. 2020;11:6343 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 2b
Jensen I, Jensen S, Sjaastad F, Gibson Corley K, Dileepan T, Griffith T, et al. Sepsis impedes EAE disease development and diminishes autoantigen-specific naive CD4 T cells. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Fernandes R, Li C, Wang G, Yang X, Savvides C, Glassman C, et al. Discovery of surrogate agonists for visceral fat Treg cells that modulate metabolic indices in vivo. elife. 2020;9: pubmed publisher
  • immunohistochemistry; mouse; loading ...
Chitirala P, Chang H, Martzloff P, Harenberg C, Ravichandran K, Abdulreda M, et al. Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...
Ning Y, Ding J, Sun X, Xie Y, Su M, Ma C, et al. HDAC9 deficiency promotes tumor progression by decreasing the CD8+ dendritic cell infiltration of the tumor microenvironment. J Immunother Cancer. 2020;8: pubmed publisher
  • flow cytometry; human; 1:75; loading ...
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
  • immunohistochemistry; mouse; fig 4h
Zheng D, Gao F, Cheng Q, Bao P, Dong X, Fan J, et al. A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11:1985 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Zhu M, Ma Y, Tan K, Zhang L, Wang Z, Li Y, et al. Thalidomide with blockade of co-stimulatory molecules prolongs the survival of alloantigen-primed mice with cardiac allografts. BMC Immunol. 2020;21:19 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b, 3e
Tashita C, Hoshi M, Hirata A, Nakamoto K, Ando T, Hattori T, et al. Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice. World J Gastroenterol. 2020;26:918-932 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s3d
Wuggenig P, Kaya B, Melhem H, Ayata C, Hruz P, Sayan A, et al. Loss of the branched-chain amino acid transporter CD98hc alters the development of colonic macrophages in mice. Commun Biol. 2020;3:130 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3b
Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
  • flow cytometry; mouse; 5 ug/ml; loading ...; fig 2j, 3a, s3a, s12r
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Canel M, Taggart D, Sims A, Lonergan D, Waizenegger I, Serrels A. T-cell co-stimulation in combination with targeting FAK drives enhanced anti-tumor immunity. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s16c
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7b
Hayes M, Ward S, Crawford G, Seoane R, Jackson W, Kipling D, et al. Inflammation-induced IgE promotes epithelial hyperplasia and tumour growth. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a, s4e
Thiele Née Schrewe L, Guse K, Tietz S, Remlinger J, Demir S, Pedreiturria X, et al. Functional relevance of the multi-drug transporter abcg2 on teriflunomide therapy in an animal model of multiple sclerosis. J Neuroinflammation. 2020;17:9 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1
Eastman A, Xu J, Bermik J, Potchen N, den Dekker A, Neal L, et al. Epigenetic stabilization of DC and DC precursor classical activation by TNFα contributes to protective T cell polarization. Sci Adv. 2019;5:eaaw9051 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e10
Majer O, Liu B, Kreuk L, Krogan N, Barton G. UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity. Nature. 2019;575:366-370 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s5a, s6a
Ansaldo E, Slayden L, Ching K, Koch M, Wolf N, Plichta D, et al. Akkermansia muciniphila induces intestinal adaptive immune responses during homeostasis. Science. 2019;364:1179-1184 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5a
Palacio L, Goyer M, Maggiorani D, Espinosa A, Villeneuve N, Bourbonnais S, et al. Restored immune cell functions upon clearance of senescence in the irradiated splenic environment. Aging Cell. 2019;18:e12971 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Sharma N, Vacher J, Allison J. TLR1/2 ligand enhances antitumor efficacy of CTLA-4 blockade by increasing intratumoral Treg depletion. Proc Natl Acad Sci U S A. 2019;116:10453-10462 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Takagaki S, Yamashita R, Hashimoto N, Sugihara K, Kanari K, Tabata K, et al. Galactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow. Sci Rep. 2019;9:7133 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s7
Knox T, Sahakian E, Banik D, Hadley M, Palmer E, Noonepalle S, et al. Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells. Sci Rep. 2019;9:6136 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3e
Jin C, Lagoudas G, Zhao C, Bullman S, Bhutkar A, Hu B, et al. Commensal Microbiota Promote Lung Cancer Development via γδ T Cells. Cell. 2019;176:998-1013.e16 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3b
Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
  • flow cytometry; mouse; fig 4f
Silva D, Yu S, Ulge U, Spangler J, Jude K, Labao Almeida C, et al. De novo design of potent and selective mimics of IL-2 and IL-15. Nature. 2019;565:186-191 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c, 2d
Maseda D, Banerjee A, Johnson E, Washington M, Kim H, Lau K, et al. mPGES-1-Mediated Production of PGE2 and EP4 Receptor Sensing Regulate T Cell Colonic Inflammation. Front Immunol. 2018;9:2954 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Cornelissen L, Blanas A, van der Horst J, Kruijssen L, Zaal A, O Toole T, et al. Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis. Int J Cancer. 2019;144:2290-2302 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5b
Ruscetti M, Leibold J, Bott M, Fennell M, Kulick A, Salgado N, et al. NK cell-mediated cytotoxicity contributes to tumor control by a cytostatic drug combination. Science. 2018;362:1416-1422 pubmed publisher
  • flow cytometry; mouse; fig 2a
Ishizuka J, Manguso R, Cheruiyot C, Bi K, Panda A, Iracheta Vellve A, et al. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature. 2019;565:43-48 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Wiedemann G, Aithal C, Kraechan A, Heise C, Cadilha B, Zhang J, et al. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma. Transl Oncol. 2019;12:350-360 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Inoue T, Ito Y, Nishizawa N, Eshima K, Kojo K, Otaka F, et al. RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis. PLoS ONE. 2018;13:e0200432 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Williams G, Schonhoff A, Jurkuvenaite A, Thome A, Standaert D, Harms A. Targeting of the class II transactivator attenuates inflammation and neurodegeneration in an alpha-synuclein model of Parkinson's disease. J Neuroinflammation. 2018;15:244 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 3e
Rezende R, Lanser A, Rubino S, Kuhn C, Skillin N, Moreira T, et al. γδ T cells control humoral immune response by inducing T follicular helper cell differentiation. Nat Commun. 2018;9:3151 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s19
Vendetti F, Karukonda P, Clump D, Teo T, Lalonde R, Nugent K, et al. ATR kinase inhibitor AZD6738 potentiates CD8+ T cell-dependent antitumor activity following radiation. J Clin Invest. 2018;128:3926-3940 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3g
Jun H, Yu H, Gong J, Jiang J, Qiao X, Perkey E, et al. An immune-beige adipocyte communication via nicotinic acetylcholine receptor signaling. Nat Med. 2018;24:814-822 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Crosby E, Wei J, Yang X, Lei G, Wang T, Liu C, et al. Complimentary mechanisms of dual checkpoint blockade expand unique T-cell repertoires and activate adaptive anti-tumor immunity in triple-negative breast tumors. Oncoimmunology. 2018;7:e1421891 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s11
Foerster F, Boegel S, Heck R, Pickert G, R ssel N, Rosigkeit S, et al. Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells. Oncoimmunology. 2018;7:e1409929 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Prado C, Gaiazzi M, Gonzalez H, Ugalde V, Figueroa A, Osorio Barrios F, et al. Dopaminergic Stimulation of Myeloid Antigen-Presenting Cells Attenuates Signal Transducer and Activator of Transcription 3-Activation Favouring the Development of Experimental Autoimmune Encephalomyelitis. Front Immunol. 2018;9:571 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s12a
Sui P, Wiesner D, Xu J, Zhang Y, Lee J, Van Dyken S, et al. Pulmonary neuroendocrine cells amplify allergic asthma responses. Science. 2018;360: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s5c
Sun H, Lagarrigue F, Gingras A, Fan Z, Ley K, Ginsberg M. Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development. J Cell Biol. 2018;217:1453-1465 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 3c
Yeh C, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018;9:928 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Tinoco R, Carrette F, Henriquez M, Fujita Y, Bradley L. Fucosyltransferase Induction during Influenza Virus Infection Is Required for the Generation of Functional Memory CD4+ T Cells. J Immunol. 2018;200:2690-2702 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 9a
Kim I, Kim K, Lee E, Oh D, Park C, Park S, et al. Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality. J Exp Med. 2018;215:963-983 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 1a
Zemmour D, Zilionis R, Kiner E, Klein A, Mathis D, Benoist C. Single-cell gene expression reveals a landscape of regulatory T cell phenotypes shaped by the TCR. Nat Immunol. 2018;19:291-301 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Fahl S, Coffey F, Kain L, Zarin P, Dunbrack R, Teyton L, et al. Role of a selecting ligand in shaping the murine γδ-TCR repertoire. Proc Natl Acad Sci U S A. 2018;115:1889-1894 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
King E, Mazor R, Cuburu N, Pastan I. Low-Dose Methotrexate Prevents Primary and Secondary Humoral Immune Responses and Induces Immune Tolerance to a Recombinant Immunotoxin. J Immunol. 2018;200:2038-2045 pubmed publisher
  • flow cytometry; mouse; fig 9f
Capucha T, Koren N, Nassar M, Heyman O, Nir T, Levy M, et al. Sequential BMP7/TGF-β1 signaling and microbiota instruct mucosal Langerhans cell differentiation. J Exp Med. 2018;215:481-500 pubmed publisher
  • flow cytometry; mouse; fig 1a
Xiao X, Fan Y, Li J, Zhang X, Lou X, Dou Y, et al. Guidance of super-enhancers in regulation of IL-9 induction and airway inflammation. J Exp Med. 2018;215:559-574 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5b
Mazor R, King E, Onda M, Cuburu N, Addissie S, Crown D, et al. Tolerogenic nanoparticles restore the antitumor activity of recombinant immunotoxins by mitigating immunogenicity. Proc Natl Acad Sci U S A. 2018;115:E733-E742 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Medaglia C, Giladi A, Stoler Barak L, De Giovanni M, Salame T, Biram A, et al. Spatial reconstruction of immune niches by combining photoactivatable reporters and scRNA-seq. Science. 2017;358:1622-1626 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Ernszt D, Banfai K, Kellermayer Z, Pap A, Lord J, Pongracz J, et al. PPARgamma Deficiency Counteracts Thymic Senescence. Front Immunol. 2017;8:1515 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2
Harms A, Thome A, Yan Z, Schonhoff A, Williams G, Li X, et al. Peripheral monocyte entry is required for alpha-Synuclein induced inflammation and Neurodegeneration in a model of Parkinson disease. Exp Neurol. 2018;300:179-187 pubmed publisher
  • flow cytometry; mouse; fig s2
Wasiuk A, Testa J, Weidlick J, Sisson C, Vitale L, Widger J, et al. CD27-Mediated Regulatory T Cell Depletion and Effector T Cell Costimulation Both Contribute to Antitumor Efficacy. J Immunol. 2017;199:4110-4123 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Zhang S, Takaku M, Zou L, Gu A, Chou W, Zhang G, et al. Reversing SKI-SMAD4-mediated suppression is essential for TH17 cell differentiation. Nature. 2017;551:105-109 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Akiel M, Guo C, Li X, Rajasekaran D, Mendoza R, Robertson C, et al. IGFBP7 Deletion Promotes Hepatocellular Carcinoma. Cancer Res. 2017;77:4014-4025 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Xie M, Koh B, Hollister K, Wu H, Sun J, Kaplan M, et al. Bcl6 promotes follicular helper T-cell differentiation and PD-1 expression in a Blimp1-independent manner in mice. Eur J Immunol. 2017;47:1136-1141 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s3a
Hasan Z, Koizumi S, Sasaki D, Yamada H, Arakaki N, Fujihara Y, et al. JunB is essential for IL-23-dependent pathogenicity of Th17 cells. Nat Commun. 2017;8:15628 pubmed publisher
  • flow cytometry; mouse; fig 1a
Taylor S, Huang Y, Mallett G, Stathopoulou C, Felizardo T, Sun M, et al. PD-1 regulates KLRG1+ group 2 innate lymphoid cells. J Exp Med. 2017;214:1663-1678 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, et al. BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J Exp Med. 2017;214:1313-1331 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3d
Sektioglu I, Carretero R, Bulbuc N, Bald T, Tüting T, Rudensky A, et al. Basophils Promote Tumor Rejection via Chemotaxis and Infiltration of CD8+ T Cells. Cancer Res. 2017;77:291-302 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 8c
Chopra M, Biehl M, Steinfatt T, Brandl A, Kums J, Amich J, et al. Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med. 2016;213:1881-900 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Damle S, Martin R, Cross J, Conrad D. Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis. Mucosal Immunol. 2017;10:205-214 pubmed publisher
Park J, Lee D, Yang S, Jeong H, Na H, Cho K, et al. Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells. J Transl Med. 2022;20:85 pubmed publisher
Almutairi F, Sarr D, Tucker S, Fantone K, Lee J, Rada B. RGS10 Reduces Lethal Influenza Infection and Associated Lung Inflammation in Mice. Front Immunol. 2021;12:772288 pubmed publisher
Li H, Wang Z, Chen Z, Ci T, Chen G, Wen D, et al. Disrupting tumour vasculature and recruitment of aPDL1-loaded platelets control tumour metastasis. Nat Commun. 2021;12:2773 pubmed publisher
Joseph R, Soundararajan R, Vasaikar S, Yang F, Allton K, Tian L, et al. CD8+ T cells inhibit metastasis and CXCL4 regulates its function. Br J Cancer. 2021;125:176-189 pubmed publisher
Huang W, Zhou S, He X, Chiem K, Mabrouk M, Nissly R, et al. SARS-CoV-2 RBD Neutralizing Antibody Induction is Enhanced by Particulate Vaccination. Adv Mater. 2020;32:e2005637 pubmed publisher
Namai F, Shigemori S, Ogita T, Sato T, Shimosato T. Microbial therapeutics for acute colitis based on genetically modified Lactococcus lactis hypersecreting IL-1Ra in mice. Exp Mol Med. 2020;52:1627-1636 pubmed publisher
Barberio A, Smith S, Correa S, Nguyen C, Nhan B, Melo M, et al. Cancer Cell Coating Nanoparticles for Optimal Tumor-Specific Cytokine Delivery. ACS Nano. 2020;14:11238-11253 pubmed publisher
Runge E, Iyer A, Setter D, Kennedy F, Sanders V, Jones K. CD4+ T cell expression of the IL-10 receptor is necessary for facial motoneuron survival after axotomy. J Neuroinflammation. 2020;17:121 pubmed publisher
von Roemeling C, Wang Y, Qie Y, Yuan H, Zhao H, Liu X, et al. Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity. Nat Commun. 2020;11:1508 pubmed publisher
Biram A, Winter E, Denton A, Zaretsky I, Dassa B, Bemark M, et al. B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches. Cell Rep. 2020;30:1910-1922.e5 pubmed publisher
Horková V, Drobek A, Mueller D, Gubser C, Niederlova V, Wyss L, et al. Dynamics of the Coreceptor-LCK Interactions during T Cell Development Shape the Self-Reactivity of Peripheral CD4 and CD8 T Cells. Cell Rep. 2020;30:1504-1514.e7 pubmed publisher
Meyer L, Huang B, Delgado Martín C, Roy R, Hechmer A, Wandler A, et al. Glucocorticoids paradoxically facilitate steroid resistance in T cell acute lymphoblastic leukemias and thymocytes. J Clin Invest. 2020;130:863-876 pubmed publisher
Sendler M, van den Brandt C, Glaubitz J, Wilden A, Golchert J, Weiss F, et al. NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis. Gastroenterology. 2020;158:253-269.e14 pubmed publisher
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product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry, IHC-F, ICC
Cat # :
100423
Clone :
GK1.5
Item :
Alexa Fluor® 488 anti-mouse CD4
Isotype :
Rat IgG2b, κ
Other Names :
L3T4, T4
Size :
100 μg
Price (USD) :
229 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A488
Immunogen :
Mouse CTL clone V4
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of CD4 + T cell activation 1,4,11 , thymocyte costimulation 3 , in vitro and in vivo depletion 2,5-8 , blocking of egg-sperm cell adhesion 1,4 , immunohistochemical staining of acetone-fixed frozen sections 9,10 , immunoprecipitation 1,2 , and spatial biology (IBEX) 12,13 . The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA