product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-mouse CD4
catalog :
100413
quantity :
25 μg
price :
94 USD
clonality :
monoclonal
host :
rat
conjugate :
APC/Cy7
clone name :
GK1.5
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 75
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 5 ug/ml; loading ...; fig 2j, 3a, s3a, s12r
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
Wilson A, Randall K, Pettitt J, Ellyard J, Blumenthal A, Enders A, et al. Neutrophil extracellular traps and their histones promote Th17 cell differentiation directly via TLR2. Nat Commun. 2022;13:528 pubmed publisher
Yu X, Bai Y, Han B, Ju M, Tang T, Shen L, et al. Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. J Extracell Vesicles. 2022;11:e12185 pubmed publisher
Jorapur A, Marshall L, Jacobson S, Xu M, Marubayashi S, Zibinsky M, et al. EBV+ tumors exploit tumor cell-intrinsic and -extrinsic mechanisms to produce regulatory T cell-recruiting chemokines CCL17 and CCL22. PLoS Pathog. 2022;18:e1010200 pubmed publisher
Shi R, Zhao K, Wang T, Yuan J, Zhang D, Xiang W, et al. 5-aza-2'-deoxycytidine potentiates anti-tumor immunity in colorectal peritoneal metastasis by modulating ABC A9-mediated cholesterol accumulation in macrophages. Theranostics. 2022;12:875-890 pubmed publisher
Fan L, Xu G, Cao J, Li M, Zhang H, Li F, et al. Type I Interferon Promotes Antitumor T Cell Response in CRPC by Regulating MDSC. Cancers (Basel). 2021;13: pubmed publisher
Molina M, Hoffman E, Stokes J, Kummet N, Smith K, Baker F, et al. Regulatory Dendritic Cells Induced by Bendamustine Are Associated With Enhanced Flt3 Expression and Alloreactive T-Cell Death. Front Immunol. 2021;12:699128 pubmed publisher
Dong L, Chen C, Zhang Y, Guo P, Wang Z, Li J, et al. The loss of RNA N6-adenosine methyltransferase Mettl14 in tumor-associated macrophages promotes CD8+ T cell dysfunction and tumor growth. Cancer Cell. 2021;39:945-957.e10 pubmed publisher
Zhang H, Dai D, Hu Q, Yang F, Xue Y, Li F, et al. Bach2 attenuates IL-2R signaling to control Treg homeostasis and Tfr development. Cell Rep. 2021;35:109096 pubmed publisher
Murdock B, Famie J, Piecuch C, Raue K, Mendelson F, Pieroni C, et al. NK cells associate with ALS in a sex- and age-dependent manner. JCI Insight. 2021;6: pubmed publisher
Vaena S, Chakraborty P, Lee H, Janneh A, Kassir M, Beeson G, et al. Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell response. Cell Rep. 2021;35:109076 pubmed publisher
Liu Y, Liang G, Xu H, Dong W, Dong Z, Qiu Z, et al. Tumors exploit FTO-mediated regulation of glycolytic metabolism to evade immune surveillance. Cell Metab. 2021;33:1221-1233.e11 pubmed publisher
Dallari S, Heaney T, Rosas Villegas A, Neil J, Wong S, Brown J, et al. Enteric viruses evoke broad host immune responses resembling those elicited by the bacterial microbiome. Cell Host Microbe. 2021;29:1014-1029.e8 pubmed publisher
Lutes L, Steier Z, McIntyre L, Pandey S, Kaminski J, Hoover A, et al. T cell self-reactivity during thymic development dictates the timing of positive selection. elife. 2021;10: pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Sheng J, Chen Q, Wu X, Dong Y, Mayer J, Zhang J, et al. Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population. elife. 2021;10: pubmed publisher
Chen J, Sivan U, Tan S, Lippo L, De Angelis J, Labella R, et al. High-resolution 3D imaging uncovers organ-specific vascular control of tissue aging. Sci Adv. 2021;7: pubmed publisher
Boyd D, Allen E, Randolph A, Guo X, Weng Y, Sanders C, et al. Exuberant fibroblast activity compromises lung function via ADAMTS4. Nature. 2020;587:466-471 pubmed publisher
Xiang W, Shi R, Zhang D, Kang X, Zhang L, Yuan J, et al. Dietary fats suppress the peritoneal seeding of colorectal cancer cells through the TLR4/Cxcl10 axis in adipose tissue macrophages. Signal Transduct Target Ther. 2020;5:239 pubmed publisher
Zhang C, Xu Z, Yang S, Sun G, Jia L, Zheng Z, et al. tagHi-C Reveals 3D Chromatin Architecture Dynamics during Mouse Hematopoiesis. Cell Rep. 2020;32:108206 pubmed publisher
Merlo L, Bowers J, Stefanoni T, Getts R, Mandik Nayak L. B-Cell-Targeted 3DNA Nanotherapy Against Indoleamine 2,3-Dioxygenase 2 (IDO2) Ameliorates Autoimmune Arthritis in a Preclinical Model. Clin Pathol. 2020;13:2632010X20951812 pubmed publisher
Liang J, Wang H, Ding W, Huang J, Zhou X, Wang H, et al. Nanoparticle-enhanced chemo-immunotherapy to trigger robust antitumor immunity. Sci Adv. 2020;6:eabc3646 pubmed publisher
Ma C, Yang D, Wang B, Wu C, Wu Y, Li S, et al. Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation. Sci Adv. 2020;6:eaaz6717 pubmed publisher
Venturutti L, Teater M, Zhai A, Chadburn A, Babiker L, Kim D, et al. TBL1XR1 Mutations Drive Extranodal Lymphoma by Inducing a Pro-tumorigenic Memory Fate. Cell. 2020;182:297-316.e27 pubmed publisher
Perry J, Tian S, Sengottuvel N, Harrison E, Gorentla B, Kapadia C, et al. Pulmonary Delivery of Nanoparticle-Bound Toll-like Receptor 9 Agonist for the Treatment of Metastatic Lung Cancer. ACS Nano. 2020;14:7200-7215 pubmed publisher
Yeung F, Chen Y, Lin J, Leung J, McCauley C, Devlin J, et al. Altered Immunity of Laboratory Mice in the Natural Environment Is Associated with Fungal Colonization. Cell Host Microbe. 2020;27:809-822.e6 pubmed publisher
Vasanthakumar A, Chisanga D, Blume J, Gloury R, Britt K, Henstridge D, et al. Sex-specific adipose tissue imprinting of regulatory T cells. Nature. 2020;579:581-585 pubmed publisher
Krautler N, Yermanos A, Pedrioli A, Welten S, Lorgé D, Greczmiel U, et al. Quantitative and Qualitative Analysis of Humoral Immunity Reveals Continued and Personalized Evolution in Chronic Viral Infection. Cell Rep. 2020;30:997-1012.e6 pubmed publisher
Aldon Y, Kratochvil S, Shattock R, McKay P. Chemokine-Adjuvanted Plasmid DNA Induces Homing of Antigen-Specific and Non-Antigen-Specific B and T Cells to the Intestinal and Genital Mucosae. J Immunol. 2020;204:903-913 pubmed publisher
Field C, Baixauli F, Kyle R, Puleston D, Cameron A, Sanin D, et al. Mitochondrial Integrity Regulated by Lipid Metabolism Is a Cell-Intrinsic Checkpoint for Treg Suppressive Function. Cell Metab. 2020;31:422-437.e5 pubmed publisher
Strickley J, Messerschmidt J, Awad M, Li T, Hasegawa T, Ha D, et al. Immunity to commensal papillomaviruses protects against skin cancer. Nature. 2019;: pubmed publisher
Schadt L, Sparano C, Schweiger N, Silina K, Cecconi V, Lucchiari G, et al. Cancer-Cell-Intrinsic cGAS Expression Mediates Tumor Immunogenicity. Cell Rep. 2019;29:1236-1248.e7 pubmed publisher
Pham D, Moseley C, Gao M, Savic D, Winstead C, Sun M, et al. Batf Pioneers the Reorganization of Chromatin in Developing Effector T Cells via Ets1-Dependent Recruitment of Ctcf. Cell Rep. 2019;29:1203-1220.e7 pubmed publisher
Chun E, Lavoie S, Fonseca Pereira D, Bae S, Michaud M, Hoveyda H, et al. Metabolite-Sensing Receptor Ffar2 Regulates Colonic Group 3 Innate Lymphoid Cells and Gut Immunity. Immunity. 2019;51:871-884.e6 pubmed publisher
Ershaid N, Sharon Y, Doron H, Raz Y, Shani O, Cohen N, et al. NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis. Nat Commun. 2019;10:4375 pubmed publisher
Wolf Y, Bartok O, Patkar S, Eli G, Cohen S, Litchfield K, et al. UVB-Induced Tumor Heterogeneity Diminishes Immune Response in Melanoma. Cell. 2019;179:219-235.e21 pubmed publisher
Viny A, Bowman R, Liu Y, Lavallée V, Eisman S, Xiao W, et al. Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation. Cell Stem Cell. 2019;25:682-696.e8 pubmed publisher
Wang G, Zhang L, Zhao X, Gao S, Qu L, Yu H, et al. The Aryl hydrocarbon receptor mediates tobacco-induced PD-L1 expression and is associated with response to immunotherapy. Nat Commun. 2019;10:1125 pubmed publisher
Farsakoglu Y, Palomino Segura M, Latino I, Zanaga S, Chatziandreou N, Pizzagalli D, et al. Influenza Vaccination Induces NK-Cell-Mediated Type-II IFN Response that Regulates Humoral Immunity in an IL-6-Dependent Manner. Cell Rep. 2019;26:2307-2315.e5 pubmed publisher
Kang Y, Biswas A, Field M, Snapper S. STAT1 signaling shields T cells from NK cell-mediated cytotoxicity. Nat Commun. 2019;10:912 pubmed publisher
Treger R, Pope S, Kong Y, Tokuyama M, Taura M, Iwasaki A. The Lupus Susceptibility Locus Sgp3 Encodes the Suppressor of Endogenous Retrovirus Expression SNERV. Immunity. 2019;50:334-347.e9 pubmed publisher
Ouyang S, Zeng Q, Tang N, Guo H, Tang R, Yin W, et al. Akt-1 and Akt-2 Differentially Regulate the Development of Experimental Autoimmune Encephalomyelitis by Controlling Proliferation of Thymus-Derived Regulatory T Cells. J Immunol. 2019;202:1441-1452 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Lavoie S, Conway K, Lassen K, Jijon H, Pan H, Chun E, et al. The Crohn's disease polymorphism, ATG16L1 T300A, alters the gut microbiota and enhances the local Th1/Th17 response. elife. 2019;8: pubmed publisher
Chen S, Fang L, Guo W, Zhou Y, Yu G, Li W, et al. Control of Treg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells. Nat Commun. 2018;9:5298 pubmed publisher
Cheng Y, Tang X, Li Y, Zhao D, Cao Q, Wu H, et al. Depression-Induced Neuropeptide Y Secretion Promotes Prostate Cancer Growth by Recruiting Myeloid Cells. Clin Cancer Res. 2019;25:2621-2632 pubmed publisher
Sade Feldman M, Yizhak K, Bjorgaard S, Ray J, de Boer C, Jenkins R, et al. Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma. Cell. 2018;175:998-1013.e20 pubmed publisher
Young J, Yin D, Vannier A, Alegre M, Chong A. Equal Expansion of Endogenous Transplant-Specific Regulatory T Cell and Recruitment Into the Allograft During Rejection and Tolerance. Front Immunol. 2018;9:1385 pubmed publisher
Tippimanchai D, Nolan K, Poczobutt J, Verzosa G, Li H, Scarborough H, et al. Adenoviral vectors transduce alveolar macrophages in lung cancer models. Oncoimmunology. 2018;7:e1438105 pubmed publisher
Shao B, Fu X, Yu Y, Yang D. Regulatory effects of miRNA‑181a on FasL expression in bone marrow mesenchymal stem cells and its effect on CD4+T lymphocyte apoptosis in estrogen deficiency‑induced osteoporosis. Mol Med Rep. 2018;18:920-930 pubmed publisher
Porrello A, Leslie P, Harrison E, Gorentla B, Kattula S, Ghosh S, et al. Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking. Nat Commun. 2018;9:1988 pubmed publisher
Li A, Barsoumian H, Schoenhals J, Cushman T, Caetano M, Wang X, et al. Indoleamine 2,3-dioxygenase 1 inhibition targets anti-PD1-resistant lung tumors by blocking myeloid-derived suppressor cells. Cancer Lett. 2018;431:54-63 pubmed publisher
Zhao N, Cao J, Xu L, Tang Q, Dobrolecki L, Lv X, et al. Pharmacological targeting of MYC-regulated IRE1/XBP1 pathway suppresses MYC-driven breast cancer. J Clin Invest. 2018;128:1283-1299 pubmed publisher
Hirata Y, Furuhashi K, Ishii H, Li H, Pinho S, Ding L, et al. CD150high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine. Cell Stem Cell. 2018;22:445-453.e5 pubmed publisher
Kunimoto H, Meydan C, Nazir A, Whitfield J, Shank K, Rapaport F, et al. Cooperative Epigenetic Remodeling by TET2 Loss and NRAS Mutation Drives Myeloid Transformation and MEK Inhibitor Sensitivity. Cancer Cell. 2018;33:44-59.e8 pubmed publisher
Petursdottir D, Nordlander S, Qazi K, Carvalho Queiroz C, Ahmed Osman O, Hell E, et al. Early-Life Human Microbiota Associated With Childhood Allergy Promotes the T Helper 17 Axis in Mice. Front Immunol. 2017;8:1699 pubmed publisher
Kleppe M, Koche R, Zou L, van Galen P, Hill C, Dong L, et al. Dual Targeting of Oncogenic Activation and Inflammatory Signaling Increases Therapeutic Efficacy in Myeloproliferative Neoplasms. Cancer Cell. 2018;33:29-43.e7 pubmed publisher
Chatterjee S, Daenthanasanmak A, Chakraborty P, Wyatt M, Dhar P, Selvam S, et al. CD38-NAD+Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response. Cell Metab. 2018;27:85-100.e8 pubmed publisher
Ma W, Liu Q, Yang J, Yang Y, Zhao Z, Ma H, et al. A Mouse Model of Autoimmune Cholangitis via Syngeneic Bile Duct Protein Immunization. Sci Rep. 2017;7:15246 pubmed publisher
Liang Z, Zhang L, Su H, Luan R, Na N, Sun L, et al. MTOR signaling is essential for the development of thymic epithelial cells and the induction of central immune tolerance. Autophagy. 2017;: pubmed publisher
Brown S, Smith C, Meuth A, Khan M, Aroor A, Cleeton H, et al. Dipeptidyl Peptidase-4 Inhibition With Saxagliptin Ameliorates Angiotensin II-Induced Cardiac Diastolic Dysfunction in Male Mice. Endocrinology. 2017;158:3592-3604 pubmed publisher
Perez Mazliah D, Nguyen M, Hosking C, McLaughlin S, Lewis M, Tumwine I, et al. Follicular Helper T Cells are Essential for the Elimination of Plasmodium Infection. EBioMedicine. 2017;24:216-230 pubmed publisher
St Clair J, Detanico T, Aviszus K, Kirchenbaum G, Christie M, Carpenter J, et al. Immunogenicity of Isogenic IgG in Aggregates and Immune Complexes. PLoS ONE. 2017;12:e0170556 pubmed publisher
Lee L, Ji M, Sinha M, Dong M, Ren X, Wang Y, et al. Determinants of Divergent Adaptive Immune Responses after Airway Sensitization with Ligands of Toll-Like Receptor 5 or Toll-Like Receptor 9. PLoS ONE. 2016;11:e0167693 pubmed publisher
Ciarlo E, Heinonen T, Herderschee J, Fenwick C, Mombelli M, Le Roy D, et al. Impact of the microbial derived short chain fatty acid propionate on host susceptibility to bacterial and fungal infections in vivo. Sci Rep. 2016;6:37944 pubmed publisher
Sen D, Jones S, Oswald E, Pinkard H, Corbin K, Krummel M. Tracking the Spatial and Functional Gradient of Monocyte-To-Macrophage Differentiation in Inflamed Lung. PLoS ONE. 2016;11:e0165064 pubmed publisher
Collinson Pautz M, Slawin K, Levitt J, Spencer D. MyD88/CD40 Genetic Adjuvant Function in Cutaneous Atypical Antigen-Presenting Cells Contributes to DNA Vaccine Immunogenicity. PLoS ONE. 2016;11:e0164547 pubmed publisher
Montes De Oca M, Kumar R, de Labastida Rivera F, Amante F, Sheel M, Faleiro R, et al. Blimp-1-Dependent IL-10 Production by Tr1 Cells Regulates TNF-Mediated Tissue Pathology. PLoS Pathog. 2016;12:e1005398 pubmed publisher
Poczobutt J, Nguyen T, Hanson D, Li H, Sippel T, Weiser Evans M, et al. Deletion of 5-Lipoxygenase in the Tumor Microenvironment Promotes Lung Cancer Progression and Metastasis through Regulating T Cell Recruitment. J Immunol. 2016;196:891-901 pubmed publisher
Holvoet B, Quattrocelli M, Belderbos S, Pollaris L, Wolfs E, Gheysens O, et al. Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice. Stem Cell Reports. 2015;5:1183-1195 pubmed publisher
Jeon Y, Na H, Ryu H, Chung Y. Modulation of Dendritic Cell Activation and Subsequent Th1 Cell Polarization by Lidocaine. PLoS ONE. 2015;10:e0139845 pubmed publisher
Chen S, Wang L, Fan J, Ye C, Dominguez D, Zhang Y, et al. Host miR155 promotes tumor growth through a myeloid-derived suppressor cell-dependent mechanism. Cancer Res. 2015;75:519-31 pubmed publisher
Mizraji G, Segev H, Wilensky A, Hovav A. Isolation, processing and analysis of murine gingival cells. J Vis Exp. 2013;:e50388 pubmed publisher
McNamee E, Masterson J, Jedlicka P, Collins C, Williams I, Rivera Nieves J. Ectopic lymphoid tissue alters the chemokine gradient, increases lymphocyte retention and exacerbates murine ileitis. Gut. 2013;62:53-62 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry
Cat # :
100413
Clone :
GK1.5
Item :
APC/Cyanine7 anti-mouse CD4
Isotype :
Rat IgG2b, κ
Other Names :
L3T4, T4
Size :
25 μg
Price (USD) :
94 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
Mouse CTL clone V4
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of CD4 + T cell activation 1,4,11 , thymocyte costimulation 3 , in vitro and in vivo depletion 2,5-8 , blocking of egg-sperm cell adhesion 1,4 , immunohistochemical staining of acetone-fixed frozen sections 9,10 , immunoprecipitation 1,2 , and spatial biology (IBEX) 12,13 . The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA