product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-mouse CD4
catalog :
100405
quantity :
50 μg
price :
28 USD
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
GK1.5
reactivity :
mouse
application :
immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 114
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 3c, 4a
Lu J, Wang W, Li P, Wang X, Gao C, Zhang B, et al. MiR-146a regulates regulatory T cells to suppress heart transplant rejection in mice. Cell Death Discov. 2021;7:165 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Gvozdeva O, Achasova K, Litvinova N, Kozhevnikova E, Litvinova E. Female Scent Activated Expression of Arginase1 and Inducible NO-Synthetase in Lung of BALB/c Male Mice. Animals (Basel). 2021;11: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Zhu M, Ma Y, Tan K, Zhang L, Wang Z, Li Y, et al. Thalidomide with blockade of co-stimulatory molecules prolongs the survival of alloantigen-primed mice with cardiac allografts. BMC Immunol. 2020;21:19 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b, 3e
Tashita C, Hoshi M, Hirata A, Nakamoto K, Ando T, Hattori T, et al. Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice. World J Gastroenterol. 2020;26:918-932 pubmed publisher
  • flow cytometry; mouse; 5 ug/ml; loading ...; fig 2j, 3a, s3a, s12r
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c, 2d
Maseda D, Banerjee A, Johnson E, Washington M, Kim H, Lau K, et al. mPGES-1-Mediated Production of PGE2 and EP4 Receptor Sensing Regulate T Cell Colonic Inflammation. Front Immunol. 2018;9:2954 pubmed publisher
  • flow cytometry; mouse
Meng Y, Zhou W, Jin L, Liu L, Chang K, Mei J, et al. RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization. Cell Death Dis. 2017;8:e3105 pubmed publisher
  • flow cytometry; mouse; loading ...
He W, Wang C, Mu R, Liang P, Huang Z, Zhang J, et al. MiR-21 is required for anti-tumor immune response in mice: an implication for its bi-directional roles. Oncogene. 2017;36:4212-4223 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Zan H, Tat C, Qiu Z, Taylor J, Guerrero J, Shen T, et al. Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination. Nat Commun. 2017;8:14244 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Cecchinato V, Bernasconi E, Speck R, Proietti M, Sauermann U, D Agostino G, et al. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization. J Immunol. 2017;198:184-195 pubmed
  • flow cytometry; mouse; 1:200; loading ...
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
  • flow cytometry; mouse; fig 5
Li Y, Nishikawa T, Kaneda Y. Platelet-cytokine Complex Suppresses Tumour Growth by Exploiting Intratumoural Thrombin-dependent Platelet Aggregation. Sci Rep. 2016;6:25077 pubmed publisher
  • flow cytometry; mouse; fig s1
Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
  • flow cytometry; mouse; fig 5
Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, et al. Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection. Eur J Immunol. 2016;46:464-79 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6a
Cui Z, Han D, Sun X, Zhang M, Feng X, Sun C, et al. Mannose-modified chitosan microspheres enhance OprF-OprI-mediated protection of mice against Pseudomonas aeruginosa infection via induction of mucosal immunity. Appl Microbiol Biotechnol. 2015;99:667-80 pubmed publisher
Zeng Q, Song J, Wang D, Sun X, Xiao Y, Zhang H, et al. Identification of Sorafenib as a Treatment for Type 1 Diabetes. Front Immunol. 2022;13:740805 pubmed publisher
Volberding P, Xin G, Kasmani M, Khatun A, Brown A, Nguyen C, et al. Suppressive neutrophils require PIM1 for metabolic fitness and survival during chronic viral infection. Cell Rep. 2021;35:109160 pubmed publisher
Dá Mesquita S, Herz J, Wall M, Dykstra T, de Lima K, Norris G, et al. Aging-associated deficit in CCR7 is linked to worsened glymphatic function, cognition, neuroinflammation, and β-amyloid pathology. Sci Adv. 2021;7: pubmed publisher
Altshuler A, Amitai Lange A, Tarazi N, Dey S, Strinkovsky L, Hadad Porat S, et al. Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing. Cell Stem Cell. 2021;28:1248-1261.e8 pubmed publisher
Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
Zhang Y, Qin Y, Chen Z. Neuromedin U Suppresses Collagen-Induced Arthritis through ILC2-Th2 Activation. J Immunol Res. 2021;2021:5599439 pubmed publisher
Ma X, Xiao L, Liu L, Ye L, Su P, Bi E, et al. CD36-mediated ferroptosis dampens intratumoral CD8+ T cell effector function and impairs their antitumor ability. Cell Metab. 2021;33:1001-1012.e5 pubmed publisher
Rosenkranz S, Shaposhnykov A, Träger S, Engler J, Witte M, Roth V, et al. Enhancing mitochondrial activity in neurons protects against neurodegeneration in a mouse model of multiple sclerosis. elife. 2021;10: pubmed publisher
Wei Z, Zhang X, Yong T, Bie N, Zhan G, Li X, et al. Boosting anti-PD-1 therapy with metformin-loaded macrophage-derived microparticles. Nat Commun. 2021;12:440 pubmed publisher
Shannon J, Vrba S, Reynoso G, Wynne Jones E, Kamenyeva O, Malo C, et al. Group 1 innate lymphoid-cell-derived interferon-γ maintains anti-viral vigilance in the mucosal epithelium. Immunity. 2021;54:276-290.e5 pubmed publisher
Martomo S, Lu D, Polonskaya Z, Luna X, Zhang Z, Feldstein S, et al. Single-Dose Anti-PD-L1/IL-15 Fusion Protein KD033 Generates Synergistic Antitumor Immunity with Robust Tumor-Immune Gene Signatures and Memory Responses. Mol Cancer Ther. 2021;20:347-356 pubmed publisher
Guo S, Smeltz R, Nanajian A, Heller R. IL-15/IL-15Rα Heterodimeric Complex as Cancer Immunotherapy in Murine Breast Cancer Models. Front Immunol. 2020;11:614667 pubmed publisher
Tang Y, Xiao Z, Pan L, Zhuang D, Cho K, Robert K, et al. Therapeutic Targeting of Retinal Immune Microenvironment With CSF-1 Receptor Antibody Promotes Visual Function Recovery After Ischemic Optic Neuropathy. Front Immunol. 2020;11:585918 pubmed publisher
Wang X, Wilhelm J, Li W, Li S, Wang Z, Huang G, et al. Polycarbonate-based ultra-pH sensitive nanoparticles improve therapeutic window. Nat Commun. 2020;11:5828 pubmed publisher
Simoni L, Presumey J, van der Poel C, Castrillón C, Chang S, Utz P, et al. Complement C4A Regulates Autoreactive B Cells in Murine Lupus. Cell Rep. 2020;33:108330 pubmed publisher
Jaeger N, McDonough R, Rosen A, Hernandez Leyva A, Wilson N, Lint M, et al. Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell Rep. 2020;33:108331 pubmed publisher
Cortez M, Masrorpour F, Ivan C, Zhang J, Younes A, Lu Y, et al. Bone morphogenetic protein 7 promotes resistance to immunotherapy. Nat Commun. 2020;11:4840 pubmed publisher
Faust H, Zhang H, Han J, Wolf M, Jeon O, Sadtler K, et al. IL-17 and immunologically induced senescence regulate response to injury in osteoarthritis. J Clin Invest. 2020;130:5493-5507 pubmed publisher
Cervera Carrascon V, Quixabeira D, Santos J, Havunen R, Zafar S, Hemminki O, et al. Tumor microenvironment remodeling by an engineered oncolytic adenovirus results in improved outcome from PD-L1 inhibition. Oncoimmunology. 2020;9:1761229 pubmed publisher
Si J, Shi X, Sun S, Zou B, Li Y, An D, et al. Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies. Cancer Cell. 2020;38:551-566.e11 pubmed publisher
Blaszczak A, Bernier M, Wright V, Gebhardt G, Anandani K, Liu J, et al. Obesogenic Memory Maintains Adipose Tissue Inflammation and Insulin Resistance. Immunometabolism. 2020;2: pubmed publisher
Wu J, Weisshaar N, Hotz Wagenblatt A, Madi A, Ma S, Mieg A, et al. Skeletal muscle antagonizes antiviral CD8+ T cell exhaustion. Sci Adv. 2020;6:eaba3458 pubmed publisher
Kamata T, So T, Ahmed Q, Giblett S, Patel B, Luo J, et al. Fibroblast-Derived STC-1 Modulates Tumor-Associated Macrophages and Lung Adenocarcinoma Development. Cell Rep. 2020;31:107802 pubmed publisher
He C, Huang X, Zhang Y, Lin X, Li S. T-cell activation and immune memory enhancement induced by irreversible electroporation in pancreatic cancer. Clin Transl Med. 2020;10:e39 pubmed publisher
Ramanan D, Sefik E, Galván Peña S, Wu M, Yang L, Yang Z, et al. An Immunologic Mode of Multigenerational Transmission Governs a Gut Treg Setpoint. Cell. 2020;181:1276-1290.e13 pubmed publisher
Sarapulov A, Petrov P, Hernández Pérez S, Sustar V, Kuokkanen E, Cords L, et al. Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses. Front Immunol. 2020;11:599 pubmed publisher
Ligon M, Wang C, DeJong E, Schulz C, Bowdish D, Mysorekar I. Single cell and tissue-transcriptomic analysis of murine bladders reveals age- and TNFα-dependent but microbiota-independent tertiary lymphoid tissue formation. Mucosal Immunol. 2020;13:908-918 pubmed publisher
Chang Y, Ko B, Ju J, Chang H, Huang S, Lin C. SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation. Int J Mol Sci. 2020;21: pubmed publisher
Wang Z, Zhao M, Yin J, Liu L, Hu L, Huang Y, et al. E4BP4-mediated inhibition of T follicular helper cell differentiation is compromised in autoimmune diseases. J Clin Invest. 2020;130:3717-3733 pubmed publisher
Aguilera T, Elghonaimy E, Shehade H, Rafat M, Castellini L, Jiang D, et al. Induced Tumor Heterogeneity Reveals Factors Informing Radiation and Immunotherapy Combinations. Clin Cancer Res. 2020;26:2972-2985 pubmed publisher
Wu N, Cernysiov V, Davidson D, Song H, Tang J, Luo S, et al. Critical Role of Lipid Scramblase TMEM16F in Phosphatidylserine Exposure and Repair of Plasma Membrane after Pore Formation. Cell Rep. 2020;30:1129-1140.e5 pubmed publisher
Hidalgo San Jose L, Sunshine M, Dillingham C, Chua B, Krutá M, Hong Y, et al. Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal. Cell Rep. 2020;30:69-80.e6 pubmed publisher
Zhao Y, Lu F, Ye J, Ji M, Pang Y, Wang Y, et al. Myeloid-Derived Suppressor Cells and γδT17 Cells Contribute to the Development of Gastric MALT Lymphoma in H. felis-Infected Mice. Front Immunol. 2019;10:3104 pubmed publisher
Hong Y, Kim Y, Kim G, Nam G, Kim S, Park Y, et al. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity. J Extracell Vesicles. 2019;8:1670893 pubmed publisher
Sivapatham S, Ficht X, Barreto de Albuquerque J, Page N, Merkler D, Stein J. Initial Viral Inoculum Determines Kinapse-and Synapse-Like T Cell Motility in Reactive Lymph Nodes. Front Immunol. 2019;10:2086 pubmed publisher
Wang X, Sun G, Feng T, Zhang J, Huang X, Wang T, et al. Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression. Cell Res. 2019;29:787-803 pubmed publisher
Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
Ji L, Zhao X, Zhang B, Kang L, Song W, Zhao B, et al. Slc6a8-Mediated Creatine Uptake and Accumulation Reprogram Macrophage Polarization via Regulating Cytokine Responses. Immunity. 2019;51:272-284.e7 pubmed publisher
Thomas S, Kuncheria L, Roulstone V, Kyula J, Mansfield D, Bommareddy P, et al. Development of a new fusion-enhanced oncolytic immunotherapy platform based on herpes simplex virus type 1. J Immunother Cancer. 2019;7:214 pubmed publisher
Kim J, Lim I, Joo H, Park C, Choi S, Jeong H, et al. Fimasartan reduces neointimal formation and inflammation after carotid arterial injury in apolipoprotein E knockout mice. Mol Med. 2019;25:33 pubmed publisher
Yang B, Wang K, Wan S, Liang Y, Yuan X, Dong Y, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27:3629-3645.e6 pubmed publisher
Timilshina M, You Z, Lacher S, Acharya S, Jiang L, Kang Y, et al. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep. 2019;27:2948-2961.e7 pubmed publisher
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Blaszczak A, Wright V, Anandani K, Liu J, Jalilvand A, Bergin S, et al. Loss of Antigen Presentation in Adipose Tissue Macrophages or in Adipocytes, but Not Both, Improves Glucose Metabolism. J Immunol. 2019;202:2451-2459 pubmed publisher
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Katsumura K, Mehta C, Hewitt K, Soukup A, Fraga de Andrade I, Ranheim E, et al. Human leukemia mutations corrupt but do not abrogate GATA-2 function. Proc Natl Acad Sci U S A. 2018;115:E10109-E10118 pubmed publisher
Xia Y, Xie Y, Yu Z, Xiao H, Jiang G, Zhou X, et al. The Mevalonate Pathway Is a Druggable Target for Vaccine Adjuvant Discovery. Cell. 2018;175:1059-1073.e21 pubmed publisher
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Habtetsion T, Ding Z, Pi W, Li T, Lu C, Chen T, et al. Alteration of Tumor Metabolism by CD4+ T Cells Leads to TNF-α-Dependent Intensification of Oxidative Stress and Tumor Cell Death. Cell Metab. 2018;28:228-242.e6 pubmed publisher
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Khosravi N, Caetano M, Cumpian A, Unver N, De la Garza Ramos C, Noble O, et al. IL22 Promotes Kras-Mutant Lung Cancer by Induction of a Protumor Immune Response and Protection of Stemness Properties. Cancer Immunol Res. 2018;6:788-797 pubmed publisher
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Nechama M, Kwon J, Wei S, Kyi A, Welner R, Ben Dov I, et al. The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation. Nat Commun. 2018;9:1603 pubmed publisher
Habib S, El Andaloussi A, Elmasry K, Handoussa A, Azab M, Elsawey A, et al. PDL-1 Blockade Prevents T Cell Exhaustion, Inhibits Autophagy, and Promotes Clearance of Leishmania donovani. Infect Immun. 2018;86: pubmed publisher
Zhou T, Zhu M, Liang Z. (-)-Epigallocatechin-3-gallate modulates peripheral immunity in the MPTP-induced mouse model of Parkinson's disease. Mol Med Rep. 2018;17:4883-4888 pubmed publisher
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Zongyi Y, Funian Z, Hao L, Xin W, Ying C, Jialin Z, et al. Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio. PLoS ONE. 2017;12:e0189617 pubmed publisher
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Valanparambil R, Tam M, Gros P, Auger J, Segura M, Gros P, et al. IRF-8 regulates expansion of myeloid-derived suppressor cells and Foxp3+ regulatory T cells and modulates Th2 immune responses to gastrointestinal nematode infection. PLoS Pathog. 2017;13:e1006647 pubmed publisher
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Timilshina M, Kang Y, Dahal I, You Z, Nam T, Kim K, et al. BJ-3105, a 6-Alkoxypyridin-3-ol Analog, Impairs T Cell Differentiation and Prevents Experimental Autoimmune Encephalomyelitis Disease Progression. PLoS ONE. 2017;12:e0168942 pubmed publisher
Aguilera T, Rafat M, Castellini L, Shehade H, Kariolis M, Hui A, et al. Reprogramming the immunological microenvironment through radiation and targeting Axl. Nat Commun. 2016;7:13898 pubmed publisher
Hart B, Lee S. Overexpression of a Mycobacterium ulcerans Ag85B-EsxH Fusion Protein in Recombinant BCG Improves Experimental Buruli Ulcer Vaccine Efficacy. PLoS Negl Trop Dis. 2016;10:e0005229 pubmed publisher
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Kim D, Lee S, Jun H. Impact of T-cell-specific Smad4 deficiency on the development of autoimmune diabetes in NOD mice. Immunol Cell Biol. 2017;95:287-296 pubmed publisher
Vigeland C, Collins S, Chan Li Y, Hughes A, Oh M, Powell J, et al. Deletion of mTORC1 Activity in CD4+ T Cells Is Associated with Lung Fibrosis and Increased γδ T Cells. PLoS ONE. 2016;11:e0163288 pubmed publisher
Ahmari N, Schmidt J, Krane G, Malphurs W, Cunningham B, Owen J, et al. Loss of bone marrow adrenergic beta 1 and 2 receptors modifies transcriptional networks, reduces circulating inflammatory factors, and regulates blood pressure. Physiol Genomics. 2016;48:526-36 pubmed publisher
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Uddback I, Pedersen L, Pedersen S, Steffensen M, Holst P, Thomsen A, et al. Combined local and systemic immunization is essential for durable T-cell mediated heterosubtypic immunity against influenza A virus. Sci Rep. 2016;6:20137 pubmed publisher
Ito C, Okuyama Dobashi K, Miyasaka T, Masuda C, Sato M, Kawano T, et al. CD8+ T Cells Mediate Female-Dominant IL-4 Production and Airway Inflammation in Allergic Asthma. PLoS ONE. 2015;10:e0140808 pubmed publisher
Hart B, Hale L, Lee S. Recombinant BCG Expressing Mycobacterium ulcerans Ag85A Imparts Enhanced Protection against Experimental Buruli ulcer. PLoS Negl Trop Dis. 2015;9:e0004046 pubmed publisher
White C, Pone E, Lam T, Tat C, Hayama K, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50 pubmed publisher
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product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry, IHC-F, ICC
Cat # :
100405
Clone :
GK1.5
Item :
FITC anti-mouse CD4
Isotype :
Rat IgG2b, κ
Other Names :
L3T4, T4
Size :
50 μg
Price (USD) :
28 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
FITC
Immunogen :
Mouse CTL clone V4
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of CD4 + T cell activation 1,4,11 , thymocyte costimulation 3 , in vitro and in vivo depletion 2,5-8 , blocking of egg-sperm cell adhesion 1,4 , immunohistochemical staining of acetone-fixed frozen sections 9,10 , immunoprecipitation 1,2 , and spatial biology (IBEX) 12,13 . The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA