product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Biotin anti-mouse CD4
catalog :
100404
quantity :
500 μg
price :
95 USD
clonality :
monoclonal
host :
rat
conjugate :
biotin
clone name :
GK1.5
reactivity :
mouse
application :
immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 25
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 3c
Yeh C, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018;9:928 pubmed publisher
  • immunocytochemistry; mouse; 1:800
Boulay A, Mazeraud A, Cisternino S, Saubaméa B, Mailly P, Jourdren L, et al. Immune quiescence of the brain is set by astroglial connexin 43. J Neurosci. 2015;35:4427-39 pubmed publisher
Khaw Y, Cunningham C, Tierney A, Sivaguru M, Inoue M. Neutrophil-selective deletion of Cxcr2 protects against CNS neurodegeneration in a mouse model of multiple sclerosis. J Neuroinflammation. 2020;17:49 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Cheng H, Onder L, Novković M, Soneson C, Lütge M, Pikor N, et al. Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp. Nat Commun. 2019;10:1739 pubmed publisher
Matsumura T, Ikebe T, Arikawa K, Hosokawa M, Aiko M, Iguchi A, et al. Sequential Sensing by TLR2 and Mincle Directs Immature Myeloid Cells to Protect against Invasive Group A Streptococcal Infection in Mice. Cell Rep. 2019;27:561-571.e6 pubmed publisher
Lin H, He C, Koprivsek J, Chen J, Zhou Z, Arulanandam B, et al. Antigen-Specific CD4+ T Cell-Derived Gamma Interferon Is Both Necessary and Sufficient for Clearing Chlamydia from the Small Intestine but Not the Large Intestine. Infect Immun. 2019;87: pubmed publisher
Zhang Z, Huang Z, Ong B, Sahu C, Zeng H, Ruan H. Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis. Haematologica. 2019;: pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Vasamsetti S, Florentin J, Coppin E, Stiekema L, Zheng K, Nisar M, et al. Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation. Immunity. 2018;49:93-106.e7 pubmed publisher
Wang Z, Yin W, Zhu L, Li J, Yao Y, Chen F, et al. Iron Drives T Helper Cell Pathogenicity by Promoting RNA-Binding Protein PCBP1-Mediated Proinflammatory Cytokine Production. Immunity. 2018;49:80-92.e7 pubmed publisher
Barry K, Hsu J, Broz M, Cueto F, Binnewies M, Combes A, et al. A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments. Nat Med. 2018;24:1178-1191 pubmed publisher
Sevin M, Kubovcakova L, Pernet N, Causse S, Vitte F, Villeval J, et al. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. Nat Commun. 2018;9:1431 pubmed publisher
Li Q, Li D, Zhang X, Wan Q, Zhang W, Zheng M, et al. E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor. Immunity. 2018;48:258-270.e5 pubmed publisher
Duarte D, Hawkins E, Akinduro O, Ang H, De Filippo K, Kong I, et al. Inhibition of Endosteal Vascular Niche Remodeling Rescues Hematopoietic Stem Cell Loss in AML. Cell Stem Cell. 2018;22:64-77.e6 pubmed publisher
Miyazaki M, Miyazaki K, Chen K, Jin Y, Turner J, Moore A, et al. The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development. Immunity. 2017;46:818-834.e4 pubmed publisher
Bian Y, Shang S, Siddiqui S, Zhao J, Joosten S, Ottenhoff T, et al. MHC Ib molecule Qa-1 presents Mycobacterium tuberculosis peptide antigens to CD8+ T cells and contributes to protection against infection. PLoS Pathog. 2017;13:e1006384 pubmed publisher
Han X, Xue X, Zhao Y, Li Y, Liu W, Zhang J, et al. Rutin-Enriched Extract from Coriandrum sativum L. Ameliorates Ionizing Radiation-Induced Hematopoietic Injury. Int J Mol Sci. 2017;18: pubmed publisher
Zhu J, Cifuentes H, Reynolds J, Lamba D. Immunosuppression via Loss of IL2rγ Enhances Long-Term Functional Integration of hESC-Derived Photoreceptors in the Mouse Retina. Cell Stem Cell. 2017;20:374-384.e5 pubmed publisher
Van De Velde L, Guo X, Barbaric L, Smith A, Oguin T, Thomas P, et al. Stress Kinase GCN2 Controls the Proliferative Fitness and Trafficking of Cytotoxic T Cells Independent of Environmental Amino Acid Sensing. Cell Rep. 2016;17:2247-2258 pubmed publisher
Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
Muro R, Nitta T, Okada T, Ideta H, Tsubata T, Suzuki H. The Ras GTPase-activating protein Rasal3 supports survival of naive T cells. PLoS ONE. 2015;10:e0119898 pubmed publisher
Ho K, Chiang Y, Lai A, Liao N, Chang Y, Yang Yen H, et al. CBAP promotes thymocyte negative selection by facilitating T-cell receptor proximal signaling. Cell Death Dis. 2014;5:e1518 pubmed publisher
Murakami R, Denda Nagai K, Hashimoto S, Nagai S, Hattori M, Irimura T. A unique dermal dendritic cell subset that skews the immune response toward Th2. PLoS ONE. 2013;8:e73270 pubmed publisher
Inamine A, Sakurai D, Horiguchi S, Yonekura S, Hanazawa T, Hosokawa H, et al. Sublingual administration of Lactobacillus paracasei KW3110 inhibits Th2-dependent allergic responses via upregulation of PD-L2 on dendritic cells. Clin Immunol. 2012;143:170-9 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
Flow Cytometry, IHC-F, ICC
Cat # :
100404
Clone :
GK1.5
Item :
Biotin anti-mouse CD4
Isotype :
Rat IgG2b, κ
Other Names :
L3T4, T4
Size :
500 μg
Price (USD) :
95 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BIOTIN
Immunogen :
Mouse CTL clone V4
Application Notes :
Additional reported applications (for the relevant formats) include: blocking of CD4 + T cell activation 1,4,11 , thymocyte costimulation 3 , in vitro and in vivo depletion 2,5-8 , blocking of egg-sperm cell adhesion 1,4 , immunohistochemical staining of acetone-fixed frozen sections 9,10 , and immunoprecipitation 1,2 . The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA