Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

PerCP/Fire™ 780 anti-mouse CD3ε

100375

25 μg

141 USD

monoclonal

hamsters

nonconjugated

145-2C11

FC

100375

145-2C11

PerCP/Fire™ 780 anti-mouse CD3ε

Armenian Hamster IgG

CD3ε, T3, CD3

25 μg

141 USD

Mouse

Monoclonal

Armenian Hamster

PERCP_F780

H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis^1, as well as T cell activation assays, inducing proliferation and cytokine production^1,2,7,12,16. It also induces apoptosis in immature thymocytes^32, and in vivo T cell depletion^8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^1, immunohistochemical staining^14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting^4, complement-mediated cytotoxicity^6, in vitro and in vivo stimulation of T cells^1,2,7,12,16, immunofluorescent staining^5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells^11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;