product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-mouse CD3ε
catalog :
100372
quantity :
100 mg
price :
3383 USD
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
145-2C11
reactivity :
mouse
application :
western blot, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 111
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:300; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; loading ...
Gvozdeva O, Achasova K, Litvinova N, Kozhevnikova E, Litvinova E. Female Scent Activated Expression of Arginase1 and Inducible NO-Synthetase in Lung of BALB/c Male Mice. Animals (Basel). 2021;11: pubmed publisher
  • flow cytometry; mouse; loading ...
Yoon S, Song S, Shin J, Kang S, Kim H, You H. Protective Effects of Korean Herbal Remedy against Airway Inflammation in an Allergic Asthma by Suppressing Eosinophil Recruitment and Infiltration in Lung. Antioxidants (Basel). 2020;10: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Ray S, Chee L, Matson D, Palermo N, Bresnick E, Hewitt K. Sterile α-motif domain requirement for cellular signaling and survival. J Biol Chem. 2020;295:7113-7125 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Eastman A, Xu J, Bermik J, Potchen N, den Dekker A, Neal L, et al. Epigenetic stabilization of DC and DC precursor classical activation by TNFα contributes to protective T cell polarization. Sci Adv. 2019;5:eaaw9051 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Takagaki S, Yamashita R, Hashimoto N, Sugihara K, Kanari K, Tabata K, et al. Galactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow. Sci Rep. 2019;9:7133 pubmed publisher
Yao R, Zhao P, Li Z, Liu Y, Zheng L, Duan Y, et al. Single-cell transcriptome profiling of sepsis identifies HLA-DRlowS100Ahigh monocytes with immunosuppressive function. Mil Med Res. 2023;10:27 pubmed publisher
Roy S, Fitzgerald K, Lalani A, Lai C, Kim A, Kim J, et al. Autonomous IL-36R signaling in neutrophils activates potent antitumor effector functions. J Clin Invest. 2023;133: pubmed publisher
Wang T, Zhao R, Zhi J, Liu Z, Wu A, Yang Z, et al. Tox4 regulates transcriptional elongation and reinitiation during murine T cell development. Commun Biol. 2023;6:613 pubmed publisher
Vereertbrugghen A, Pizzano M, Sabbione F, Keitelman I, Shiromizu C, Aguilar D, et al. An ocular Th1 immune response promotes corneal nerve damage independently of the development of corneal epitheliopathy. J Neuroinflammation. 2023;20:120 pubmed publisher
Xiao X, Shi J, He C, Bu X, Sun Y, Gao M, et al. ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy. Nat Commun. 2023;14:2859 pubmed publisher
Wang Y, Qin H, Cai Y, Chen X, Li H, Montoya Durango D, et al. Natural γδT17 cell development and functional acquisition is governed by the mTORC2-c-Maf-controlled mitochondrial fission pathway. iScience. 2023;26:106630 pubmed publisher
Pandit M, Kil Y, Ahn J, Pokhrel R, Gu Y, Mishra S, et al. Methionine consumption by cancer cells drives a progressive upregulation of PD-1 expression in CD4 T cells. Nat Commun. 2023;14:2593 pubmed publisher
Mabire M, Hegde P, Hammoutene A, Wan J, Ca xeb r C, Sayegh R, et al. MAIT cell inhibition promotes liver fibrosis regression via macrophage phenotype reprogramming. Nat Commun. 2023;14:1830 pubmed publisher
Guo L, Wang W, Wang Q, Hao D, Ito M, Huang B, et al. The adrenal stress response is an essential host response against therapy-induced lethal immune activation. Sci Signal. 2023;16:eadd4900 pubmed publisher
Li X, Li Y, Dong L, Chang Y, Zhang X, Wang C, et al. Decitabine priming increases anti-PD-1 antitumor efficacy by promoting CD8+ progenitor exhausted T cell expansion in tumor models. J Clin Invest. 2023;133: pubmed publisher
Aso K, Kono M, Kanda M, Kudo Y, Sakiyama K, Hisada R, et al. Itaconate ameliorates autoimmunity by modulating T cell imbalance via metabolic and epigenetic reprogramming. Nat Commun. 2023;14:984 pubmed publisher
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Romine K, Macpherson K, Cho H, Kosaka Y, Flynn P, Byrd K, et al. BET inhibitors rescue anti-PD1 resistance by enhancing TCF7 accessibility in leukemia-derived terminally exhausted CD8+ T cells. Leukemia. 2023;37:580-592 pubmed publisher
Yang H, Zhang X, Lao M, Sun K, He L, Xu J, et al. Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer. Cell Death Differ. 2023;30:560-575 pubmed publisher
Wang Z, Qin X, Hu D, Huang J, Guo E, Xiao R, et al. Akkermansia supplementation reverses the tumor-promoting effect of the fecal microbiota transplantation in ovarian cancer. Cell Rep. 2022;41:111890 pubmed publisher
Sato A, Azuma M, Nagai H, Imai W, Kawaguchi K, Morita M, et al. OX40 Ligand-Mannose-Binding Lectin Fusion Protein Induces Potent OX40 Cosignaling in CD4+ T Cells. Biol Pharm Bull. 2022;45:1798-1804 pubmed publisher
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Denk D, Petrocelli V, Conche C, Drachsler M, Ziegler P, Braun A, et al. Expansion of T memory stem cells with superior anti-tumor immunity by Urolithin A-induced mitophagy. Immunity. 2022;55:2059-2073.e8 pubmed publisher
Li L, Ding P, Lv X, Xie S, Li L, Chen J, et al. CD59-Regulated Ras Compartmentalization Orchestrates Antitumor T-cell Immunity. Cancer Immunol Res. 2022;10:1475-1489 pubmed publisher
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Mirji G, Worth A, Bhat S, El Sayed M, Kannan T, Goldman A, et al. The microbiome-derived metabolite TMAO drives immune activation and boosts responses to immune checkpoint blockade in pancreatic cancer. Sci Immunol. 2022;7:eabn0704 pubmed publisher
Li Q, Sun X, Yu K, Lv J, Miao C, Yang J, et al. Enterobacter ludwigii protects DSS-induced colitis through choline-mediated immune tolerance. Cell Rep. 2022;40:111308 pubmed publisher
Smith K, Minns D, McHugh B, Holloway R, O CONNOR R, Williams A, et al. The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation. PLoS Biol. 2022;20:e3001554 pubmed publisher
Potluri H, Ferreira C, Grudzinski J, Massey C, Aluicio Sarduy E, Engle J, et al. Antitumor efficacy of 90Y-NM600 targeted radionuclide therapy and PD-1 blockade is limited by regulatory T cells in murine prostate tumors. J Immunother Cancer. 2022;10: pubmed publisher
Pandit M, Timilshina M, Gu Y, Acharya S, Chung Y, Seo S, et al. AMPK suppresses Th2 cell responses by repressing mTORC2. Exp Mol Med. 2022;54:1214-1224 pubmed publisher
Gudgeon N, Munford H, Bishop E, Hill J, Fulton Ward T, Bending D, et al. Succinate uptake by T cells suppresses their effector function via inhibition of mitochondrial glucose oxidation. Cell Rep. 2022;40:111193 pubmed publisher
Pfenninger P, Yerly L, Abe J. Naïve Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering. Front Immunol. 2022;13:777113 pubmed publisher
Guo Z, Zhang J, Liu X, Unsinger J, Hotchkiss R, Cao Y. Low-dose interleukin-2 reverses chronic migraine-related sensitizations through peripheral interleukin-10 and transforming growth factor beta-1 signaling. Neurobiol Pain. 2022;12:100096 pubmed publisher
Jou E, Rodriguez Rodriguez N, Ferreira A, Jolin H, Clark P, Sawmynaden K, et al. An innate IL-25-ILC2-MDSC axis creates a cancer-permissive microenvironment for Apc mutation-driven intestinal tumorigenesis. Sci Immunol. 2022;7:eabn0175 pubmed publisher
Li X, Yong T, Wei Z, Bie N, Zhang X, Zhan G, et al. Reversing insufficient photothermal therapy-induced tumor relapse and metastasis by regulating cancer-associated fibroblasts. Nat Commun. 2022;13:2794 pubmed publisher
Takahashi F, Zhang C, Hohjoh H, Raveney B, Yamamura T, Hayashi N, et al. Immune-mediated neurodegenerative trait provoked by multimodal derepression of long-interspersed nuclear element-1. iScience. 2022;25:104278 pubmed publisher
Zhang R, Wang Y, Liu D, Luo Q, Du P, Zhang H, et al. Sodium Tanshinone IIA Sulfonate as a Potent IDO1/TDO2 Dual Inhibitor Enhances Anti-PD1 Therapy for Colorectal Cancer in Mice. Front Pharmacol. 2022;13:870848 pubmed publisher
Kwon H, Schafer J, Song N, Kaneko S, Li A, Xiao T, et al. Androgen conspires with the CD8+ T cell exhaustion program and contributes to sex bias in cancer. Sci Immunol. 2022;7:eabq2630 pubmed publisher
Ye L, Park J, Peng L, Yang Q, Chow R, Lam S, et al. A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy. Cell Metab. 2022;34:595-614.e14 pubmed publisher
Wang D, Xiu J, Zhao J, Luo J. miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell level. EMBO Rep. 2022;23:e53691 pubmed publisher
Krone A, Fu Y, Schreiber S, Kotrba J, Borde L, Nötzold A, et al. Ionic mitigation of CD4+ T cell metabolic fitness, Th1 central nervous system autoimmunity and Th2 asthmatic airway inflammation by therapeutic zinc. Sci Rep. 2022;12:1943 pubmed publisher
Gandhi V, Cephus J, Norlander A, Chowdhury N, Zhang J, Ceneviva Z, et al. Androgen receptor signaling promotes Treg suppressive function during allergic airway inflammation. J Clin Invest. 2022;132: pubmed publisher
Du Y, Peng Q, Cheng D, Pan T, Sun W, Wang H, et al. Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells. Nat Commun. 2022;13:231 pubmed publisher
Qi Z, Xu Z, Zhang L, Zou Y, Li J, Yan W, et al. Overcoming resistance to immune checkpoint therapy in PTEN-null prostate cancer by intermittent anti-PI3Kα/β/δ treatment. Nat Commun. 2022;13:182 pubmed publisher
Cheng B, Ding K, Chen P, Ji J, Luo T, Guo X, et al. Anti-PD-L1/TGF-βR fusion protein (SHR-1701) overcomes disrupted lymphocyte recovery-induced resistance to PD-1/PD-L1 inhibitors in lung cancer. Cancer Commun (Lond). 2022;42:17-36 pubmed publisher
Li H, Xiao Y, Li Q, Yao J, Yuan X, Zhang Y, et al. The allergy mediator histamine confers resistance to immunotherapy in cancer patients via activation of the macrophage histamine receptor H1. Cancer Cell. 2022;40:36-52.e9 pubmed publisher
Hajjar S, Nathan N, Joseph J, Mottawea W, Ariana A, Pyatibrat S, et al. Foxo3a tempers excessive glutaminolysis in activated T cells to prevent fatal gut inflammation in the murine IL-10-/- model of colitis. Cell Death Differ. 2022;29:585-599 pubmed publisher
Herrera F, Ronet C, Ochoa de Olza M, Barras D, Crespo I, Andreatta M, et al. Low-Dose Radiotherapy Reverses Tumor Immune Desertification and Resistance to Immunotherapy. Cancer Discov. 2022;12:108-133 pubmed publisher
Okubo A, Uchida Y, Higashi Y, Sato T, Ogawa Y, Ryuge A, et al. CD147 Is Essential for the Development of Psoriasis via the Induction of Th17 Cell Differentiation. Int J Mol Sci. 2021;23: pubmed publisher
Xu Y, Liu E, Xie X, Wang J, Zheng H, Ju Y, et al. Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases. iScience. 2021;24:103445 pubmed publisher
Zhai X, Liu K, Fang H, Zhang Q, Gao X, Liu F, et al. Mitochondrial C1qbp promotes differentiation of effector CD8+ T cells via metabolic-epigenetic reprogramming. Sci Adv. 2021;7:eabk0490 pubmed publisher
Liu Y, Debo B, Li M, Shi Z, Sheng W, Shi Y. LSD1 inhibition sustains T cell invigoration with a durable response to PD-1 blockade. Nat Commun. 2021;12:6831 pubmed publisher
Gurusamy M, Tischner D, Shao J, Klatt S, Zukunft S, Bonnavion R, et al. G-protein-coupled receptor P2Y10 facilitates chemokine-induced CD4 T cell migration through autocrine/paracrine mediators. Nat Commun. 2021;12:6798 pubmed publisher
Stump C, Roehle K, Manjarrez Orduno N, Dougan S. Radiation combines with immune checkpoint blockade to enhance T cell priming in a murine model of poorly immunogenic pancreatic cancer. Open Biol. 2021;11:210245 pubmed publisher
Jairaman A, Cahalan M. Calcium Imaging in T Lymphocytes: a Protocol for Use with Genetically Encoded or Chemical Ca2+ Indicators. Bio Protoc. 2021;11:e4170 pubmed publisher
Zhang Z, Yang C, Li L, Zhu Y, Su K, Zhai L, et al. "γδT Cell-IL17A-Neutrophil" Axis Drives Immunosuppression and Confers Breast Cancer Resistance to High-Dose Anti-VEGFR2 Therapy. Front Immunol. 2021;12:699478 pubmed publisher
Daneshmandi S, Cassel T, Higashi R, Fan T, Seth P. 6-Phosphogluconate dehydrogenase (6PGD), a key checkpoint in reprogramming of regulatory T cells metabolism and function. elife. 2021;10: pubmed publisher
Menzel L, Zschummel M, Crowley T, Franke V, Grau M, Ulbricht C, et al. Lymphocyte access to lymphoma is impaired by high endothelial venule regression. Cell Rep. 2021;37:109878 pubmed publisher
Fujiwara Y, Torphy R, Sun Y, Miller E, Ho F, Borcherding N, et al. The GPR171 pathway suppresses T cell activation and limits antitumor immunity. Nat Commun. 2021;12:5857 pubmed publisher
Zhao X, Liu J, Zhang C, Yu N, Lu Z, Zhang S, et al. Porphyromonas gingivalis exacerbates ulcerative colitis via Porphyromonas gingivalis peptidylarginine deiminase. Int J Oral Sci. 2021;13:31 pubmed publisher
Innamarato P, Morse J, Mackay A, Asby S, Beatty M, Blauvelt J, et al. Intralesional injection of rose bengal augments the efficacy of gemcitabine chemotherapy against pancreatic tumors. BMC Cancer. 2021;21:756 pubmed publisher
Glassman C, Su L, Majri Morrison S, Winkelmann H, Mo F, Li P, et al. Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist. elife. 2021;10: pubmed publisher
Luo Z, Xia K, Liu Y, Liu J, Rao S, Hu X, et al. Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8+ T Cells and Macrophages. Int J Nanomedicine. 2021;16:2949-2963 pubmed publisher
Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
Li X, Dong L, Liu J, Wang C, Zhang Y, Mei Q, et al. Low-Dose Decitabine Augments the Activation and Anti-Tumor Immune Response of IFN-γ+ CD4+ T Cells Through Enhancing IκBα Degradation and NF-κB Activation. Front Cell Dev Biol. 2021;9:647713 pubmed publisher
Yang X, Zhou F, Yuan P, Dou G, Liu X, Liu S, et al. T cell-depleting nanoparticles ameliorate bone loss by reducing activated T cells and regulating the Treg/Th17 balance. Bioact Mater. 2021;6:3150-3163 pubmed publisher
He Y, Fu L, Li Y, Wang W, Gong M, Zhang J, et al. Gut microbial metabolites facilitate anticancer therapy efficacy by modulating cytotoxic CD8+ T cell immunity. Cell Metab. 2021;33:988-1000.e7 pubmed publisher
Han K, Singh K, Rodman M, Hassanzadeh S, Wu K, Nguyen A, et al. Fasting-induced FOXO4 blunts human CD4+ T helper cell responsiveness. Nat Metab. 2021;3:318-326 pubmed publisher
Daneshmandi S, Cassel T, Lin P, Higashi R, Wulf G, Boussiotis V, et al. Blockade of 6-phosphogluconate dehydrogenase generates CD8+ effector T cells with enhanced anti-tumor function. Cell Rep. 2021;34:108831 pubmed publisher
Frost J, Tan T, Abbas M, Wideman S, Bonadonna M, Stoffel N, et al. Hepcidin-Mediated Hypoferremia Disrupts Immune Responses to Vaccination and Infection. Med. 2021;2:164-179.e12 pubmed publisher
Woo M, Ufer F, Rothammer N, Di Liberto G, Binkle L, Haferkamp U, et al. Neuronal metabotropic glutamate receptor 8 protects against neurodegeneration in CNS inflammation. J Exp Med. 2021;218: pubmed publisher
Wang F, Chau B, West S, Kimberlin C, Cao F, Schwarz F, et al. Structures of mouse and human GITR-GITRL complexes reveal unique TNF superfamily interactions. Nat Commun. 2021;12:1378 pubmed publisher
Minns D, Smith K, Alessandrini V, Hardisty G, Melrose L, Jackson Jones L, et al. The neutrophil antimicrobial peptide cathelicidin promotes Th17 differentiation. Nat Commun. 2021;12:1285 pubmed publisher
Glassman C, Mathiharan Y, Jude K, Su L, Panova O, Lupardus P, et al. Structural basis for IL-12 and IL-23 receptor sharing reveals a gateway for shaping actions on T versus NK cells. Cell. 2021;184:983-999.e24 pubmed publisher
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Saragovi A, Abramovich I, Omar I, Arbib E, Toker O, Gottlieb E, et al. Systemic hypoxia inhibits T cell response by limiting mitobiogenesis via matrix substrate-level phosphorylation arrest. elife. 2020;9: pubmed publisher
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Wang W, Li Y, Hao J, He Y, Dong X, Fu Y, et al. The Interaction between Lymphoid Tissue Inducer-Like Cells and T Cells in the Mesenteric Lymph Node Restrains Intestinal Humoral Immunity. Cell Rep. 2020;32:107936 pubmed publisher
Donado C, Cao A, Simmons D, Croker B, Brennan P, Brenner M. A Two-Cell Model for IL-1β Release Mediated by Death-Receptor Signaling. Cell Rep. 2020;31:107466 pubmed publisher
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Huang Y, Li D, Zhang P, Liu M, Liang X, Yang X, et al. IL-18R-dependent and independent pathways account for IL-18-enhanced antitumor ability of CAR-T cells. FASEB J. 2020;34:1768-1782 pubmed publisher
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Gupta S, Sharp R, Hofferek C, Kuai L, Dorn G, Wang J, et al. NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells. Cell Rep. 2019;29:1862-1877.e7 pubmed publisher
Krovi S, Kappler J, Marrack P, Gapin L. Inherent reactivity of unselected TCR repertoires to peptide-MHC molecules. Proc Natl Acad Sci U S A. 2019;116:22252-22261 pubmed publisher
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Engler J, Kursawe N, Solano M, Patas K, Wehrmann S, Heckmann N, et al. Glucocorticoid receptor in T cells mediates protection from autoimmunity in pregnancy. Proc Natl Acad Sci U S A. 2017;114:E181-E190 pubmed publisher
Dong L, Helmke A, Waisman A, Haller H, Pich A, von Vietinghoff S. Surface-bound bovine serum albumin carrier protein as present in recombinant cytokine preparations amplifies T helper 17 cell polarization. Sci Rep. 2016;6:36598 pubmed publisher
product information
Antigen :
CD3epsilon
Apps. Abbrev. :
FC, IHC-F, IP, Activ, Block, WB, ICC
Cat # :
100372
Clone :
145-2C11
Item :
Ultra-LEAF™ Purified anti-mouse CD3ε
Isotype :
Armenian Hamster IgG
Other Names :
CD3ε, T3, CD3
Size :
100 mg
Price (USD) :
3383 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
ULEAF
Immunogen :
H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37
Application Notes :
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis^1, as well as T cell activation assays, inducing proliferation and cytokine production^1,2,7,12,16. It also induces apoptosis in immature thymocytes^32, and in vivo T cell depletion^8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^1, immunohistochemical staining^14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting^4, complement-mediated cytotoxicity^6, in vitro and in vivo stimulation of T cells^1,2,7,12,16, immunofluorescent staining^5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells^11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA