Brilliant Violet 510™ anti-mouse CD3ε | BioLegend 100353 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 510™ anti-mouse CD3ε

catalog :

100353

quantity :

50 μg

price :

242 USD

clonality :

monoclonal

host :

hamsters

conjugate :

BV510

clone name :

145-2C11

reactivity :

mouse, rat

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...	Hemmi T, Ainai A, Hashiguchi T, Tobiume M, Kanno T, Iwata Yoshikawa N, et al. Intranasal vaccination induced cross-protective secretory IgA antibodies against SARS-CoV-2 variants with reducing the potential risk of lung eosinophilic immunopathology. Vaccine. 2022;40:5892-5903 pubmed publisher
flow cytometry; rat; 1:150; loading ...; fig s4b	Helms T, Mullins R, Thomas Ahner J, Kulp S, Campbell M, Lucas F, et al. Inhibition of androgen/AR signaling inhibits diethylnitrosamine (DEN) induced tumour initiation and remodels liver immune cell networks. Sci Rep. 2021;11:3646 pubmed publisher
	Cortez M, Masrorpour F, Ivan C, Zhang J, Younes A, Lu Y, et al. Bone morphogenetic protein 7 promotes resistance to immunotherapy. Nat Commun. 2020;11:4840 pubmed publisher
	Schropp V, Rohde J, Rovituso D, Jabari S, Bharti R, Kuerten S. Contribution of LTi and TH17 cells to B cell aggregate formation in the central nervous system in a mouse model of multiple sclerosis. J Neuroinflammation. 2019;16:111 pubmed publisher
	Kumar M, Du J, Lagoudas G, Jiao Y, Sawyer A, Drummond D, et al. Analysis of Single-Cell RNA-Seq Identifies Cell-Cell Communication Associated with Tumor Characteristics. Cell Rep. 2018;25:1458-1468.e4 pubmed publisher
	Cong J, Wang X, Zheng X, Wang D, Fu B, Sun R, et al. Dysfunction of Natural Killer Cells by FBP1-Induced Inhibition of Glycolysis during Lung Cancer Progression. Cell Metab. 2018;28:243-255.e5 pubmed publisher
	Sheng W, LaFleur M, Nguyen T, Chen S, Chakravarthy A, Conway J, et al. LSD1 Ablation Stimulates Anti-tumor Immunity and Enables Checkpoint Blockade. Cell. 2018;174:549-563.e19 pubmed publisher
	Niknam S, Barsoumian H, Schoenhals J, Jackson H, Yanamandra N, Caetano M, et al. Radiation Followed by OX40 Stimulation Drives Local and Abscopal Antitumor Effects in an Anti-PD1-Resistant Lung Tumor Model. Clin Cancer Res. 2018;24:5735-5743 pubmed publisher

product information

Antigen :

CD3epsilon

Apps. Abbrev. :

Flow Cytometry

Cat # :

100353

Clone :

145-2C11

Item :

Brilliant Violet 510™ anti-mouse CD3ε

Isotype :

Armenian Hamster IgG

Other Names :

CD3ε, T3, CD3

Size :

50 μg

Price (USD) :

242 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

BV510

Immunogen :

H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37

Application Notes :

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).

more info or order :

BioLegend product webpage