Brilliant Violet 605™ anti-mouse CD3ε | BioLegend 100351 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 605™ anti-mouse CD3ε

catalog :

100351

quantity :

50 μg

price :

262 USD

clonality :

monoclonal

host :

hamsters

conjugate :

BV605

clone name :

145-2C11

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:200; loading ...; fig 1s2a	Lebratti T, Lim Y, Cofie A, Andhey P, Jiang X, Scott J, et al. A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection. elife. 2021;10: pubmed publisher
flow cytometry; mouse; fig s3g	Linehan J, Harrison O, Han S, Byrd A, Vujkovic Cvijin I, Villarino A, et al. Non-classical Immunity Controls Microbiota Impact on Skin Immunity and Tissue Repair. Cell. 2018;172:784-796.e18 pubmed publisher
	Lima Júnior D, Krishnamurthy S, Bouladoux N, Collins N, Han S, Chen E, et al. Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota. Cell. 2021;184:3794-3811.e19 pubmed publisher
	Schiller M, Azulay Debby H, Boshnak N, Elyahu Y, Korin B, Ben Shaanan T, et al. Optogenetic activation of local colonic sympathetic innervations attenuates colitis by limiting immune cell extravasation. Immunity. 2021;54:1022-1036.e8 pubmed publisher
	Zhang X, SABIO E, Krishna C, Ma X, Wang J, Jiang H, et al. Qa-1b Modulates Resistance to Anti-PD-1 Immune Checkpoint Blockade in Tumors with Defects in Antigen Processing. Mol Cancer Res. 2021;19:1076-1084 pubmed publisher
	Perner C, Flayer C, Zhu X, Aderhold P, Dewan Z, Voisin T, et al. Substance P Release by Sensory Neurons Triggers Dendritic Cell Migration and Initiates the Type-2 Immune Response to Allergens. Immunity. 2020;53:1063-1077.e7 pubmed publisher
	Shapiro M, Yeh W, Longfield J, Gallagher J, Infante C, Wellford S, et al. CD226 Deletion Reduces Type 1 Diabetes in the NOD Mouse by Impairing Thymocyte Development and Peripheral T Cell Activation. Front Immunol. 2020;11:2180 pubmed publisher
	Wang J, Li Y, Wang P, Han G, Zhang T, Chang J, et al. Leukemogenic Chromatin Alterations Promote AML Leukemia Stem Cells via a KDM4C-ALKBH5-AXL Signaling Axis. Cell Stem Cell. 2020;27:81-97.e8 pubmed publisher
	Nagai M, Noguchi R, Takahashi D, Morikawa T, Koshida K, Komiyama S, et al. Fasting-Refeeding Impacts Immune Cell Dynamics and Mucosal Immune Responses. Cell. 2019;178:1072-1087.e14 pubmed publisher
	Tan L, Sandrock I, Odak I, Aizenbud Y, Wilharm A, Barros Martins J, et al. Single-Cell Transcriptomics Identifies the Adaptation of Scart1+ Vγ6+ T Cells to Skin Residency as Activated Effector Cells. Cell Rep. 2019;27:3657-3671.e4 pubmed publisher
	Sade Feldman M, Yizhak K, Bjorgaard S, Ray J, de Boer C, Jenkins R, et al. Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma. Cell. 2018;175:998-1013.e20 pubmed publisher
	Clement M, Marsden M, Stacey M, Abdul Karim J, Gimeno Brias S, Costa Bento D, et al. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016;12:e1006050 pubmed publisher
	Baglaenko Y, Manion K, Chang N, Gracey E, Loh C, Wither J. IL-10 Production Is Critical for Sustaining the Expansion of CD5+ B and NKT Cells and Restraining Autoantibody Production in Congenic Lupus-Prone Mice. PLoS ONE. 2016;11:e0150515 pubmed publisher
	Gutierrez D, Fu W, Schonefeldt S, Feyerabend T, Ortiz Lopez A, Lampi Y, et al. Type 1 diabetes in NOD mice unaffected by mast cell deficiency. Diabetes. 2014;63:3827-34 pubmed publisher
	Yan J, Villarreal D, Racine T, Chu J, Walters J, Morrow M, et al. Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine. Vaccine. 2014;32:2833-42 pubmed publisher
	Wu S, Zhang Z, Yu R, Zhang J, Liu Y, Song X, et al. Intramuscular delivery of adenovirus serotype 5 vector expressing humanized protective antigen induces rapid protection against anthrax that may bypass intranasally originated preexisting adenovirus immunity. Clin Vaccine Immunol. 2014;21:156-64 pubmed publisher
	Helmersson S, Sundstedt A, Deronic A, Leanderson T, Ivars F. Amelioration of experimental autoimmune encephalomyelitis by the quinoline-3-carboxamide paquinimod: reduced priming of proinflammatory effector CD4(+) T cells. Am J Pathol. 2013;182:1671-80 pubmed publisher
	Qi Q, Xia M, Hu J, Hicks E, Iyer A, Xiong N, et al. Enhanced development of CD4+ gammadelta T cells in the absence of Itk results in elevated IgE production. Blood. 2009;114:564-71 pubmed publisher
	Schulteis R, Chu H, Dai X, Chen Y, Edwards B, Haribhai D, et al. Impaired survival of peripheral T cells, disrupted NK/NKT cell development, and liver failure in mice lacking Gimap5. Blood. 2008;112:4905-14 pubmed publisher
	Kanaya T, Miyazawa K, Takakura I, Itani W, Watanabe K, Ohwada S, et al. Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage. Am J Physiol Gastrointest Liver Physiol. 2008;295:G273-84 pubmed publisher
	Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
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product information

Antigen :

CD3epsilon

Apps. Abbrev. :

Flow Cytometry

Cat # :

100351

Clone :

145-2C11

Item :

Brilliant Violet 605™ anti-mouse CD3ε

Isotype :

Armenian Hamster IgG

Other Names :

CD3ε, T3, CD3

Size :

50 μg

Price (USD) :

262 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

BV605

Immunogen :

H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37

Application Notes :

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).

more info or order :

BioLegend product webpage