Brilliant Violet 711™ anti-mouse CD3ε | BioLegend 100349 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 711™ anti-mouse CD3ε

catalog :

100349

quantity :

50 μg

price :

262 USD

clonality :

monoclonal

host :

hamsters

conjugate :

BV711

clone name :

145-2C11

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 5

Reference
Seydoux E, Wan Y, Feng J, Wall A, Aljedani S, Homad L, et al. Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies. Cell Rep. 2021;35:109084 pubmed publisher
Mogilenko D, Shpynov O, Andhey P, Arthur L, Swain A, Esaulova E, et al. Comprehensive Profiling of an Aging Immune System Reveals Clonal GZMK+ CD8+ T Cells as Conserved Hallmark of Inflammaging. Immunity. 2021;54:99-115.e12 pubmed publisher
Zhu X, Chudnovskiy A, Baudrier L, Prizer B, Liu Y, Ostendorf B, et al. Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells. Cell Metab. 2021;33:211-221.e6 pubmed publisher
Annu K, Cline C, Yasuda K, Ganguly S, Pesch A, Cooper B, et al. Role of Vitamins A and D in BCR-ABL Arf-/- Acute Lymphoblastic Leukemia. Sci Rep. 2020;10:2359 pubmed publisher
Tan L, Sandrock I, Odak I, Aizenbud Y, Wilharm A, Barros Martins J, et al. Single-Cell Transcriptomics Identifies the Adaptation of Scart1+ Vγ6+ T Cells to Skin Residency as Activated Effector Cells. Cell Rep. 2019;27:3657-3671.e4 pubmed publisher

product information

Antigen :

CD3epsilon

Apps. Abbrev. :

Flow Cytometry

Cat # :

100349

Clone :

145-2C11

Item :

Brilliant Violet 711™ anti-mouse CD3ε

Isotype :

Armenian Hamster IgG

Other Names :

CD3ε, T3, CD3

Size :

50 μg

Price (USD) :

262 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

BV711

Immunogen :

H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37

Application Notes :

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).

more info or order :

BioLegend product webpage