Brilliant Violet 421™ anti-mouse CD3ε | BioLegend 100336 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-mouse CD3ε

catalog :

100336

quantity :

500 μL

price :

385 USD

clonality :

monoclonal

host :

hamsters

conjugate :

BV421

clone name :

145-2C11

reactivity :

mouse

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 40

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; loading ...; fig s1a, s2g	LaFleur M, Nguyen T, Coxe M, Yates K, Trombley J, Weiss S, et al. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nat Commun. 2019;10:1668 pubmed publisher
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	Stegelmeier A, Santry L, Guilleman M, Matuszewska K, Minott J, Yates J, et al. AAV-Vectored Expression of the Vascular Normalizing Agents 3TSR and Fc3TSR, and the Anti-Angiogenic Bevacizumab Extends Survival in a Murine Model of End-Stage Epithelial Ovarian Carcinoma. Biomedicines. 2022;10: pubmed publisher
	Wilson A, Randall K, Pettitt J, Ellyard J, Blumenthal A, Enders A, et al. Neutrophil extracellular traps and their histones promote Th17 cell differentiation directly via TLR2. Nat Commun. 2022;13:528 pubmed publisher
	Ren Z, Pan L, Huang Y, Chen H, Liu Y, Liu H, et al. Gut microbiota-CRAMP axis shapes intestinal barrier function and immune responses in dietary gluten-induced enteropathy. EMBO Mol Med. 2021;13:e14059 pubmed publisher
	Mah Som A, Keppel M, Tobin J, Kolicheski A, Saucier N, Sexl V, et al. Reliance on Cox10 and oxidative metabolism for antigen-specific NK cell expansion. Cell Rep. 2021;35:109209 pubmed publisher
	Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
	Argüello R, Combes A, Char R, Gigan J, Baaziz A, Bousiquot E, et al. SCENITH: A Flow Cytometry-Based Method to Functionally Profile Energy Metabolism with Single-Cell Resolution. Cell Metab. 2020;32:1063-1075.e7 pubmed publisher
	Si Y, Tian Q, Zhao F, Kelly S, Shores L, Camacho D, et al. Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and TH17 responses. Sci Adv. 2020;6:eaba0995 pubmed publisher
	Pilones K, Charpentier M, García Martínez E, Daviaud C, Kraynak J, Aryankalayil J, et al. Radiotherapy Cooperates with IL15 to Induce Antitumor Immune Responses. Cancer Immunol Res. 2020;8:1054-1063 pubmed publisher
	Eckert E, Nace R, Tonne J, Evgin L, Vile R, Russell S. Generation of a Tumor-Specific Chemokine Gradient Using Oncolytic Vesicular Stomatitis Virus Encoding CXCL9. Mol Ther Oncolytics. 2020;16:63-74 pubmed publisher
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	Brown C, Gudjonson H, Pritykin Y, Deep D, Lavallée V, Mendoza A, et al. Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity. Cell. 2019;179:846-863.e24 pubmed publisher
	LaFleur M, Nguyen T, Coxe M, Miller B, Yates K, Gillis J, et al. PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nat Immunol. 2019;20:1335-1347 pubmed publisher
	Maluski M, Ghosh A, Herbst J, Scholl V, Baumann R, Huehn J, et al. Chimeric antigen receptor-induced BCL11B suppression propagates NK-like cell development. J Clin Invest. 2019;129:5108-5122 pubmed publisher
	Chow M, Ozga A, Servis R, Frederick D, Lo J, Fisher D, et al. Intratumoral Activity of the CXCR3 Chemokine System Is Required for the Efficacy of Anti-PD-1 Therapy. Immunity. 2019;: pubmed publisher
	Misumi I, Starmer J, Uchimura T, Beck M, Magnuson T, Whitmire J. Obesity Expands a Distinct Population of T Cells in Adipose Tissue and Increases Vulnerability to Infection. Cell Rep. 2019;27:514-524.e5 pubmed publisher
	van Vloten J, Santry L, McAusland T, Karimi K, McFadden G, Petrik J, et al. Quantifying Antigen-Specific T Cell Responses When Using Antigen-Agnostic Immunotherapies. Mol Ther Methods Clin Dev. 2019;13:154-166 pubmed publisher
	Kiyohara H, Sujino T, Teratani T, Miyamoto K, Arai M, Nomura E, et al. Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells. Cell Mol Gastroenterol Hepatol. 2019;7:135-156 pubmed publisher
	Kumar D, Rahman H, Tyagi E, Liu T, Li C, Lu R, et al. Aspirin Suppresses PGE2 and Activates AMP Kinase to Inhibit Melanoma Cell Motility, Pigmentation, and Selective Tumor Growth In Vivo. Cancer Prev Res (Phila). 2018;11:629-642 pubmed publisher
	Mrdjen D, Pavlovic A, Hartmann F, Schreiner B, Utz S, Leung B, et al. High-Dimensional Single-Cell Mapping of Central Nervous System Immune Cells Reveals Distinct Myeloid Subsets in Health, Aging, and Disease. Immunity. 2018;48:380-395.e6 pubmed publisher
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product information

Antigen :

CD3epsilon

Apps. Abbrev. :

Flow Cytometry, ICC

Cat # :

100336

Clone :

145-2C11

Item :

Brilliant Violet 421™ anti-mouse CD3ε

Isotype :

Armenian Hamster IgG

Other Names :

CD3ε, T3, CD3

Size :

500 μL

Price (USD) :

385 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

BV421

Immunogen :

H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37

Application Notes :

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).

more info or order :

BioLegend product webpage