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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-mouse CD3ε
catalog :
100330
quantity :
100 μg
price :
215 USD
clonality :
monoclonal
host :
hamsters
conjugate :
APC/Cy7
clone name :
145-2C11
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 55
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 2k
Mitroulis I, Ruppova K, Wang B, Chen L, Grzybek M, Grinenko T, et al. Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity. Cell. 2018;172:147-161.e12 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Moodley D, Yoshida H, Mostafavi S, Asinovski N, Ortiz Lopez A, Symanowicz P, et al. Network pharmacology of JAK inhibitors. Proc Natl Acad Sci U S A. 2016;113:9852-7 pubmed publisher
  • flow cytometry; mouse
Chen J, Miyanishi M, Wang S, Yamazaki S, Sinha R, Kao K, et al. Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche. Nature. 2016;530:223-7 pubmed publisher
Zeng Q, Song J, Wang D, Sun X, Xiao Y, Zhang H, et al. Identification of Sorafenib as a Treatment for Type 1 Diabetes. Front Immunol. 2022;13:740805 pubmed publisher
Yuan M, Zhai Y, Men Y, Zhao M, Sun X, Ma Z, et al. Anlotinib Enhances the Antitumor Activity of High-Dose Irradiation Combined with Anti-PD-L1 by Potentiating the Tumor Immune Microenvironment in Murine Lung Cancer. Oxid Med Cell Longev. 2022;2022:5479491 pubmed publisher
Anastasiou M, Newton G, Kaur K, Carrillo Salinas F, Smolgovsky S, Bayer A, et al. Endothelial STING controls T cell transmigration in an IFNI-dependent manner. JCI Insight. 2021;6: pubmed publisher
Mah Som A, Keppel M, Tobin J, Kolicheski A, Saucier N, Sexl V, et al. Reliance on Cox10 and oxidative metabolism for antigen-specific NK cell expansion. Cell Rep. 2021;35:109209 pubmed publisher
Zhang H, Dai D, Hu Q, Yang F, Xue Y, Li F, et al. Bach2 attenuates IL-2R signaling to control Treg homeostasis and Tfr development. Cell Rep. 2021;35:109096 pubmed publisher
Earnest J, Holmes A, Basore K, Mack M, Fremont D, Diamond M. The mechanistic basis of protection by non-neutralizing anti-alphavirus antibodies. Cell Rep. 2021;35:108962 pubmed publisher
Zhang C, Xu Z, Yang S, Sun G, Jia L, Zheng Z, et al. tagHi-C Reveals 3D Chromatin Architecture Dynamics during Mouse Hematopoiesis. Cell Rep. 2020;32:108206 pubmed publisher
Chikina A, Nadalin F, Maurin M, San Roman M, Thomas Bonafos T, Li X, et al. Macrophages Maintain Epithelium Integrity by Limiting Fungal Product Absorption. Cell. 2020;183:411-428.e16 pubmed publisher
Kong Y, Dreaden E, Morandell S, Zhou W, Dhara S, Sriram G, et al. Enhancing chemotherapy response through augmented synthetic lethality by co-targeting nucleotide excision repair and cell-cycle checkpoints. Nat Commun. 2020;11:4124 pubmed publisher
Ni J, Wang X, Stojanovic A, Zhang Q, Wincher M, Bühler L, et al. Single-Cell RNA Sequencing of Tumor-Infiltrating NK Cells Reveals that Inhibition of Transcription Factor HIF-1α Unleashes NK Cell Activity. Immunity. 2020;52:1075-1087.e8 pubmed publisher
Bayık D, Zhou Y, Park C, Hong C, Vail D, Silver D, et al. Myeloid-Derived Suppressor Cell Subsets Drive Glioblastoma Growth in a Sex-Specific Manner. Cancer Discov. 2020;10:1210-1225 pubmed publisher
Miao L, Qi J, Zhao Q, Wu Q, Wei D, Wei X, et al. Targeting the STING pathway in tumor-associated macrophages regulates innate immune sensing of gastric cancer cells. Theranostics. 2020;10:498-515 pubmed publisher
Ge Y, Gong M, Zadeh M, Li J, Abbott J, Li W, et al. Regulating colonic dendritic cells by commensal glycosylated large surface layer protein A to sustain gut homeostasis against pathogenic inflammation. Mucosal Immunol. 2020;13:34-46 pubmed publisher
Wang W, Cohen J, Wallrapp A, Trieu K, Barrios J, Shao F, et al. Age-Related Dopaminergic Innervation Augments T Helper 2-Type Allergic Inflammation in the Postnatal Lung. Immunity. 2019;51:1102-1118.e7 pubmed publisher
Yang X, Guo Y, Guo Z, Si T, Xing W, Yu W, et al. Cryoablation inhibition of distant untreated tumors (abscopal effect) is immune mediated. Oncotarget. 2019;10:4180-4191 pubmed publisher
Bommareddy P, Lowe D, Kaufman H, Rabkin S, Saha D. Multi-parametric flow cytometry staining procedure for analyzing tumor-infiltrating immune cells following oncolytic herpes simplex virus immunotherapy in intracranial glioblastoma. J Biol Methods. 2019;6: pubmed
Fachi J, Felipe J, Pral L, da Silva B, Corrêa R, de Andrade M, et al. Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism. Cell Rep. 2019;27:750-761.e7 pubmed publisher
Wang B, Lim J, Kajikawa T, Li X, Vallance B, Moutsopoulos N, et al. Macrophage β2-Integrins Regulate IL-22 by ILC3s and Protect from Lethal Citrobacter rodentium-Induced Colitis. Cell Rep. 2019;26:1614-1626.e5 pubmed publisher
Zhou J, Peng H, Li K, Qu K, Wang B, Wu Y, et al. Liver-Resident NK Cells Control Antiviral Activity of Hepatic T Cells via the PD-1-PD-L1 Axis. Immunity. 2019;50:403-417.e4 pubmed publisher
Uchil P, Pi R, Haugh K, Ladinsky M, Ventura J, Barrett B, et al. A Protective Role for the Lectin CD169/Siglec-1 against a Pathogenic Murine Retrovirus. Cell Host Microbe. 2019;25:87-100.e10 pubmed publisher
Calcinotto A, Brevi A, Chesi M, Ferrarese R, Garcia Perez L, Grioni M, et al. Microbiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression. Nat Commun. 2018;9:4832 pubmed publisher
Grzelak A, Polakova I, Smahelova J, Vackova J, Pekarčíková L, Tachezy R, et al. Experimental Combined Immunotherapy of Tumours with Major Histocompatibility Complex Class I Downregulation. Int J Mol Sci. 2018;19: pubmed publisher
Celik H, Koh W, Kramer A, Ostrander E, Mallaney C, Fisher D, et al. JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells. Cancer Cell. 2018;34:741-756.e8 pubmed publisher
Tsai S, Clemente Casares X, Zhou A, Lei H, Ahn J, Chan Y, et al. Insulin Receptor-Mediated Stimulation Boosts T Cell Immunity during Inflammation and Infection. Cell Metab. 2018;28:922-934.e4 pubmed publisher
Cong J, Wang X, Zheng X, Wang D, Fu B, Sun R, et al. Dysfunction of Natural Killer Cells by FBP1-Induced Inhibition of Glycolysis during Lung Cancer Progression. Cell Metab. 2018;28:243-255.e5 pubmed publisher
Kim M, Galan C, Hill A, Wu W, Fehlner Peach H, Song H, et al. Critical Role for the Microbiota in CX3CR1+ Intestinal Mononuclear Phagocyte Regulation of Intestinal T Cell Responses. Immunity. 2018;49:151-163.e5 pubmed publisher
Brown Z, Fu Q, Ma C, Kruhlak M, Zhang H, Luo J, et al. Carnitine palmitoyltransferase gene upregulation by linoleic acid induces CD4+ T cell apoptosis promoting HCC development. Cell Death Dis. 2018;9:620 pubmed publisher
Barbet G, Sander L, Geswell M, Leonardi I, Cerutti A, Iliev I, et al. Sensing Microbial Viability through Bacterial RNA Augments T Follicular Helper Cell and Antibody Responses. Immunity. 2018;48:584-598.e5 pubmed publisher
Colliou N, Ge Y, Gong M, Zadeh M, Li J, Alonzo F, et al. Regulation of Th17 cells by P. UF1 against systemic Listeria monocytogenes infection. Gut Microbes. 2018;9:279-287 pubmed publisher
Fu B, Zhou Y, Ni X, Tong X, Xu X, Dong Z, et al. Natural Killer Cells Promote Fetal Development through the Secretion of Growth-Promoting Factors. Immunity. 2017;47:1100-1113.e6 pubmed publisher
Palacios Arreola M, Nava Castro K, Río Araiza V, Pérez Sánchez N, Morales Montor J. A single neonatal administration of Bisphenol A induces higher tumour weight associated to changes in tumour microenvironment in the adulthood. Sci Rep. 2017;7:10573 pubmed publisher
Saha D, Martuza R, Rabkin S. Macrophage Polarization Contributes to Glioblastoma Eradication by Combination Immunovirotherapy and Immune Checkpoint Blockade. Cancer Cell. 2017;32:253-267.e5 pubmed publisher
Maston L, Jones D, Giermakowska W, Howard T, Cannon J, Wang W, et al. Central role of T helper 17 cells in chronic hypoxia-induced pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 2017;312:L609-L624 pubmed publisher
Rao S, Schieber A, O Connor C, Leblanc M, Michel D, Ayres J. Pathogen-Mediated Inhibition of Anorexia Promotes Host Survival and Transmission. Cell. 2017;168:503-516.e12 pubmed publisher
Qi S, Li H, Lu L, Qi Z, Liu L, Chen L, et al. Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy. elife. 2016;5: pubmed publisher
Campisi L, Barbet G, Ding Y, Esplugues E, Flavell R, Blander J. Apoptosis in response to microbial infection induces autoreactive TH17 cells. Nat Immunol. 2016;17:1084-92 pubmed publisher
Bonaccorsi Riani E, Danger R, Lozano J, Martinez Picola M, Kodela E, Mas Malavila R, et al. Iron Deficiency Impairs Intra-Hepatic Lymphocyte Mediated Immune Response. PLoS ONE. 2015;10:e0136106 pubmed publisher
Gutierrez D, Fu W, Schonefeldt S, Feyerabend T, Ortiz Lopez A, Lampi Y, et al. Type 1 diabetes in NOD mice unaffected by mast cell deficiency. Diabetes. 2014;63:3827-34 pubmed publisher
Yan J, Villarreal D, Racine T, Chu J, Walters J, Morrow M, et al. Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine. Vaccine. 2014;32:2833-42 pubmed publisher
Wu S, Zhang Z, Yu R, Zhang J, Liu Y, Song X, et al. Intramuscular delivery of adenovirus serotype 5 vector expressing humanized protective antigen induces rapid protection against anthrax that may bypass intranasally originated preexisting adenovirus immunity. Clin Vaccine Immunol. 2014;21:156-64 pubmed publisher
Helmersson S, Sundstedt A, Deronic A, Leanderson T, Ivars F. Amelioration of experimental autoimmune encephalomyelitis by the quinoline-3-carboxamide paquinimod: reduced priming of proinflammatory effector CD4(+) T cells. Am J Pathol. 2013;182:1671-80 pubmed publisher
Shoji M, Tachibana M, Katayama K, Tomita K, Tsuzuki S, Sakurai F, et al. Type-I IFN signaling is required for the induction of antigen-specific CD8(+) T cell responses by adenovirus vector vaccine in the gut-mucosa. Biochem Biophys Res Commun. 2012;425:89-93 pubmed publisher
Meng J, Greenlee A, Taub C, Braun R. Sertoli cell-specific deletion of the androgen receptor compromises testicular immune privilege in mice. Biol Reprod. 2011;85:254-60 pubmed publisher
Qi Q, Xia M, Hu J, Hicks E, Iyer A, Xiong N, et al. Enhanced development of CD4+ gammadelta T cells in the absence of Itk results in elevated IgE production. Blood. 2009;114:564-71 pubmed publisher
Schulteis R, Chu H, Dai X, Chen Y, Edwards B, Haribhai D, et al. Impaired survival of peripheral T cells, disrupted NK/NKT cell development, and liver failure in mice lacking Gimap5. Blood. 2008;112:4905-14 pubmed publisher
Kanaya T, Miyazawa K, Takakura I, Itani W, Watanabe K, Ohwada S, et al. Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage. Am J Physiol Gastrointest Liver Physiol. 2008;295:G273-84 pubmed publisher
Guo Y, Maillard I, Chakraborti S, Rothenberg E, Speck N. Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification. Blood. 2008;112:480-92 pubmed publisher
Chappaz S, Flueck L, Farr A, Rolink A, Finke D. Increased TSLP availability restores T- and B-cell compartments in adult IL-7 deficient mice. Blood. 2007;110:3862-70 pubmed
Perchonock C, Pajerowski A, Nguyen C, Shapiro M, Shapiro V. The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes. J Immunol. 2007;179:1768-75 pubmed
Perchonock C, Fernando M, Quinn W, Nguyen C, Sun J, Shapiro M, et al. Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006;26:6005-15 pubmed
de Koning B, van Dieren J, Lindenbergh Kortleve D, Van Der Sluis M, Matsumoto T, Yamaguchi K, et al. Contributions of mucosal immune cells to methotrexate-induced mucositis. Int Immunol. 2006;18:941-9 pubmed
Curtsinger J, Lins D, Johnson C, Mescher M. Signal 3 tolerant CD8 T cells degranulate in response to antigen but lack granzyme B to mediate cytolysis. J Immunol. 2005;175:4392-9 pubmed
product information
Antigen :
CD3epsilon
Apps. Abbrev. :
Flow Cytometry
Cat # :
100330
Clone :
145-2C11
Item :
APC/Cyanine7 anti-mouse CD3ε
Isotype :
Armenian Hamster IgG
Other Names :
CD3ε, T3, CD3
Size :
100 μg
Price (USD) :
215 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
H-2K b -specific mouse cytotoxic T lymphocyte clone BM10-37
Application Notes :
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis 1 , as well as T cell activation assays, inducing proliferation and cytokine production 1,2,7,12,16 . It also induces apoptosis in immature thymocytes 32 , and in vivo T cell depletion 8-10 . Additional reported applications (for relevant formats of this clone) include: immunoprecipitation 1 , immunohistochemical staining 14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting 4 , complement-mediated cytotoxicity 6 , in vitro and in vivo stimulation of T cells 1,2,7,12,16 , immunofluorescent staining 5 , and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells 11 . Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA